Lamb Brain Research Articles

Experiments in Border disease: VII. The disease in goats

Border disease of sheep has been reproduced in low incidence in the kid by injection of the pregnant goat with affected new born lamb brain tissue. The disease was characterized by tremor, hypomyelinogenesis and hypergliosis of the C.N.S., but abnormalities of the birth coat and granular aggregations of myelin-like lipid in the C.N.S. which also characterize the disease in newborn lambs were not seen. The rate and severity of the disease process, particularly in the placenta, appears to be greater than in sheep and this may account for the high level of abortion encountered in this and previous experiments in goats.

Calorimetric properties of lamb and other meats

Summary. A description is given of an automatic adiabatic calorimeter which was used to obtain enthalpy‐temperature data for lamb brains and kidneys, calf veal and five selected lamb cuts. Water content of the cuts correlated well with fat content and with ‘latent heats’ of freezing. Calculations indicated 11% of the total water content of low fat meat was not frozen at ‐20°F and there appeared to be a tendency for this non‐frozen portion to increase with meat of higher fat content.

Characterization of a phosphodiesterase from lamb brain

An endonuclease which hydrolyzes thermally denatured DNA has been detected in lamb brain homogenates. A unique assay which depends on the ability of the enzyme to decrease the antigenic competence of thermally denatured DNA facilitated its detection and was used during its purification. Evidence for thermally denatured DNA being the preferred substrate was obtained by: (a) release of acid-soluble P 32, (b) loss of residual transforming activity of denatured DNA, (c) disappearance of only the denatured DNA band when partially denatured DNA was treated with the endonuclease and the mixture subjected to CsCl centrifugation, and (d) loss of serologic activity. Oligonucleotides varying in chain length from 5 to 14 residues are the products of the enzymatic digestion. By analysis of the oligonucleotide products after treatment with snake venom diesterase, the site of hydrolysis was identified as the 3′ hydroxyl side of the diester bond.

Influence of phenothiazine administration on cytochrome oxidase activity in lamb brain tissue.

Influence of Phenothiazine Administration on Cytochrome Oxidase Activity in Lamb Brain Tissue

Fructose and Glucose Utilization by Lamb and Sheep Brain

Fructose can be utilized by the mammalian brain in vitro as judged by oxygen uptake and by lactate production, although with some preparations lactate production was slower from fruotose than from gluoose (LoebeI1925; Diokens and Greville 1933; Edson and Leloir 1936; Geiger 1940; Klein 1944; Meyerhof and Wilson 1948). An increase in oxygen uptake by brain slices in vitro, during application of electrical pulses to the tissue, occurs when glucose is present in the medium; this also occurs when fructose is present in the medium, but higher concentrations of fructose than gluoose are needed for the same effeot (McIlwain 1953).

Coenurosis in New Zealand

Abstract Extract Sir,—During the survey on the cestode problems of food animals and dogs in New Zealand, Multiceps multi-cops (Leske, 1780) and its metacestode Coenurus cerebralis have been observed in the dog and sheep respectively in mid-Canterbury. An unconfirmed case has also been reported recently in a lamb's brain exported to Australia.