Laboratory Parameters In Patients Research Articles

Застосування методів консервативної терапії в дітей з післяопіковими рубцевими деформаціями та їх вплив на метаболізм сполучної тканини

One of the factors of scarring is changes in the connective tissue (CT). Defeat of CT is accompanied by changes in the content of glycosaminoglycans (GAG), activity of lysosomal enzymes, levels of mineral metabolism. To eliminate the influence of inflammatory phenomena and disorders of CT metabolism on the formation of scar tissue, it is urgent to use conservative rehabilitation measures. Purpose - to determine the effectiveness of measures of conservative therapy of post-burn scars in children for the clinical course and metabolism of connective tissue. Material and methods. 27 children aged 5 to 18 were supervised. The group of healthy children without clinical manifestations of inflammatory reactions consisted of 17 people. Conservative treatment of children with scarring included local, drug, physiotherapy, balneological and efferent therapy methods. The impact of treatment measures was assessed on a modified scar score scale, which consisted of a patient (parent) scale and a researcher scale. Laboratory criteria of effectiveness were the amount of ceruloplasmin (CP), lactic (MC) and sialic (SC) acids, GAG content, lysosomal enzyme activity (bone and alkaline phosphatase), mineral metabolism (total calcium). Laboratory parameters in patients were studied in the period from 150 to 170 and 310 to 340 days after injury. SPSS v was used for statistical analysis. 17.0. Results. Improvements in clinical signs of scar tissue were 1.7-fold according to objective estimates and 1.6-fold according to patient estimates. Laboratory monitoring showed the effectiveness of conservative treatment of convalescents, which was manifested in a reduction in GAG levels 1.6 times after 6 months and 2.0 times after a year. There was a decrease in the activity of acid phosphatase in 1.6 times, alkaline phosphatase and its bone fraction in 1.4 times. In addition, after treatment, there was an increase in ceruloplasmin activity by 1.5 times at all times. A 1.5-fold increase in the number of MCs was determined at all observation times. After treatment, the amount of MC decreased by 1.2 times (P<0.05). Significant confirmation of an increase in the level of SC in 1.4 times (P<0.05) was observed only on the 160th day of the study. At other times, the increase in the IC was unreliable. Conclusions. Long-term studies have shown that in children after thermal trauma in the process of productive scarring there are deviations in the metabolism of CT in the direction of increasing its synthetic function, metabolic disorders and inflammation continue. The use of complex conservative therapy in children with post-burn scars during convalescence allowed to reduce the severity of inflammatory reactions, improve CT metabolism and reduce scarring. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Key words: post-burn scarring in children, connective tissue, metabolism, inflammation, conservative therapy.

Differential gene expression of ABCG2, SLC22A12, IL-1\u03b2, and ALPK1 in peripheral blood leukocytes of primary gout patients with hyperuricemia and their comorbidities: a case\u2013control study

BackgroundThe ABCG2, SLC22A12, and ALPK1 genes have been strongly associated with dysfunction of urate metabolism in patients with gout, but it is unknown how these transporters are expressed in patients with acute or chronic gout. Our objectives were to: (a) analyze the gene expression of urate transporters and of inflammation genes in peripheral blood from gout patients and controls; (b) determine whether the metabolic profile of gout patients can influence the gene expression profile and the expression of urate transporters, ABCG2 and SLC22A12, and inflammation molecules, ALPK1 and IL-1β, in peripheral blood leukocytes from gout patients; (c) compare them with their metabolic profile and the gene expression of people without gout and without hyperuricemia.MethodsA total of 36 chronic and acute patients and 52 controls were recruited, and ABCG2, SLC22A12, IL-1β, and ALPK1 gene expression was evaluated by quantitative real-time PCR. Correlations of gene expression with clinical and laboratory parameters of patients were also analyzed.ResultsIL-1β was significantly increased in peripheral blood mononuclear cells (PBMCs) of patients compared with their polymorphonuclear leukocytes white blood cells (PMNLs, p < 0.05). A significant increase in ABCG2 and IL-1β was found in PMNLs from patients compared to controls (p < 0.05). Correlations of gene expression in patients were found with levels of serum uric acid (sUA), serum creatinine, C-reactive protein (CRP), triglycerides, body mass index (BMI), kidney disease, hypertension, and metabolic syndrome.ConclusionsOur data suggest that leukocytes of patients respond to the presence of hyperuricemia and comorbidities, expressing ABCG2 and IL-1β genes differentially compared to normouricemic and nondisease states. Hyperuricemia, dyslipidemia, and obesity probably stimulate the differential gene expression of peripheral blood leukocytes (neutrophils and monocytes), even in an asymptomatic state.

W145 Relationships between stress hyperglycemia, mortality prevalence and some laboratory parameters in patients with acute ischemic stroke

W145 Relationships between stress hyperglycemia, mortality prevalence and some laboratory parameters in patients with acute ischemic stroke

Prediction of Stroke After the COVID-19 Infection.

Although several studies have been published about COVID-19, ischemic stroke is known yet as a complicated problem for COVID-19 patients. Scientific reports have indicated that in many cases, the incidence of stroke in patients with COVID-19 leads to death. The obtained mathematical equation in this study can help physicians' decision-making about treatment and identification of influential clinical factors for early diagnosis. In this retrospective study, data from 128 patients between March and September 2020, including their demographic information, clinical characteristics, and laboratory parameters were collected and analyzed statistically. A logistic regression model was developed to identify the significant variables in predicting stroke incidence in patients with COVID-19. Clinical characteristics and laboratory parameters for 128 patients (including 76 males and 52 females; with a mean age of 57.109±15.97 years) were considered as the inputs that included ventilator dependence, comorbidities, and laboratory tests, including WBC, neutrophil, lymphocyte, platelet count, C-reactive protein, blood urea nitrogen, alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH). Receiver operating characteristic-area under the curve (ROC-AUC), accuracy, sensitivity, and specificity were considered indices to determine the model capability. The accuracy of the model classification was also addressed by 93.8%. The area under the curve was 97.5% with a 95% CI. The findings showed that ventilator dependence, cardiac ejection fraction, and LDH are associated with the occurrence of stroke and the proposed model can predict the stroke effectively.

Association of Neutrophils and Lymphocytes Ratio with Respect to Severity of Coronary Artery Disease

Background: Cardiovascular diseases caused an estimated 17.3 million deaths in a year. Around 40% of these cardiovascular deaths are due to coronary heart disease. It is projected that coronary heart diseases will remain a leading cause of morbidity and mortality for many years to come. Atherosclerosis, the main underlying pathologic process for coronary heart diseases, is a chronic disease state of the coronary arteries that slowly develops over decades before becoming clinically significant. Its pathophysiology is complex, including inflammatory and immunological events which are considered to be of central importance in the initiation and progression of atherosclerotic plaques. Gradual chronic progression of coronary atherosclerosis may result in luminal narrowing causing symptoms of angina. Aim: To study neutrophils to lymphocyte as inflammatory markers in long standing (chronic) coronary artery disease. Material and methods: A Cross sectional Observational study was conducted at outpatient department of Medicine in Krishna Hospital and research centre. For a period October 2019- October 2021. Registration of patients was from October 2019. Such 72 cases were studied. They were registered when admitted under Medicine department. At the time of registration, the patients with exclusion criteria were not enrolled for study. The main objective of this study was to study clinical and laboratory parameters of patients with coronary artery disease, to do Coronary angiographic evaluation of cardiac functions. Results: Mean Age in Years Was 56.72+5.5. Majority 77.77% were in age group of 50 to 60 years and 70.83% were males. Patients mainly complained of chest pain 97.22%, followed by breathlessness 86.11%, palpitation 15.27%, retrosternal burning 13.88%, sweating 15.27%, tightness in chest 13.88% and tremors 13.88%. Conclusion: Study concluded that there is a strong association between NLR and severity of coronary artery disease. Among Cases having positive ..........

Role of routine laboratory markers in the diagnosis of rotavirus and adenovirus gastroenteritis

Purpose: The present study aimed to evaluate and estimate the additional and auxiliary diagnostic value of routine laboratory parameters in patients with acute diarrhea caused by rotavirus and adenovirus. Methods/ patients: A total of 6784 patients diagnosed with acute gastroenteritis were evaluated. Rotavirus and adenovirus infection was diagnosed via a Qualitative immunochromatographic combo rapid cassette antigen test. Complete blood count and biochemical blood tests were performed in all the patients and were compared between the groups according to the positivity or negativity of the virus. Results: Rotavirus diarrhea was diagnosed in 16.8% and adenovirus diarrhea in 3.2% of patients. Hemoglobin, hematocrit, and mean cell volume (MCV) levels were lower, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were significantly higher in rotavirus positive cases. Neutrophil to lymphocyte ratio was significantly higher, and lymphocyte to monocyte ratio was significantly lower in positive rotavirus cases than negative ones 2.96 and 2.56, respectively (p&lt;0.001). Conclusions: Hematological and biochemical parameters may assist in diagnosing and distinguishing rotaviral and adenoviral gastroenteritis, especially in low-resource environments. Keywords: adenovirus; children; diarrhea; laboratory markers; rotavirus

Relationship between myalgia and laboratory parameters in hospitalized patients with COVID-19

Aim: Myalgia is among the first and most common symptoms in patients with COVID-19. A limited number of studies have been found evaluating the frequency of myalgia and the laboratory findings associated with this condition. In this study, we aimed to evaluate the prevalence of myalgia and the relationship between myalgia and laboratory parameters in patients who were hospitalized due to COVID-19.&#x0D; Material and Methods: Three hundred fifty-eight patients with confirmed diagnoses of COVID-19 who were hospitalized between March 2020 and January 2021 were included in the study. The patients were divided into two groups according to the presence and absence of myalgia. Demographic characteristics, medical history, symptoms, clinical findings, and laboratory findings were evaluated retrospectively.&#x0D; Results: A total of 358 patients, 192 (42.9%) females and 166 males, were included in the study. The mean age of the patients was 60.3 ± 15.2 years. When the laboratory findings of the 166 patients with myalgia and 192 patients with no myalgia were compared, no difference was found between the groups in terms of white blood cell, neutrophil, lymphocyte, monocyte, and platelet counts, C-reactive protein, ferritin D-dimer, and troponin levels. However, creatine kinase (CK) levels were found to be significantly higher in the group with myalgia compared with the group without myalgia (p

De Ritis Ratio, Neutrophil-to-Lymphocyte Ratio, and Albumin Are Significant Prognostic Factors for Survival Even After Adjusted by the Treatment Duration in Metastatic Kidney and Bladder Cancer Treated With Immune-Checkpoint Inhibitors

Purpose: This study aimed to determine the prognostic roles of several immune-related laboratory parameters in patients with metastatic kidney and bladder cancer treated with immune checkpoint inhibitors (ICIs).Materials and Methods: Overall, 36 patients with either metastatic bladder (N=18) or kidney cancer (N=18) were enrolled retrospectively. Progression-free survival (PFS) and overall survival according to the pretherapeutic serum De Ritis ratio (DRR), neutrophil-to-lymphocyte ratio (NLR), and albumin level after ICI treatment, were analyzed. Treatment duration was adjusted using Contal and O’Quigley’s method to explore the cutoff and maximize the log-rank test statistic. Cox proportional hazards model was used to analyze the laboratory parameters.Results: A total of 9 patients received a combination therapy of multiple ICIs (N=9) and targeted agents (N=7). The median NLR, DRR, and albumin level at baseline were 1.7, 1.2, and 4.2 mg/dL, respectively. In the univariable analysis, combination of immunotherapies, total ICI cycles, baseline DRR, and albumin level were significant for PFS. Sex ratio, total ICI cycles, and baseline NLR and DRR were significant for cancer-specific survival (CSS). DRR and albumin levels, which were measured for up to 10 cycles, were significant in PFS and CSS. NLR was additionally significant in CSS. After adjusting total ICI cycles, DRR was significant in PFS and CSS, albumin level was significant only in PFS, and NLR was significant only in CSS in the multivariable analysis.Conclusions: NLR, DRR, and albumin level are significant factors associated with the survival of patients with metastatic kidney and bladder cancer treated with ICI.

Comparison of laboratory parameters in patients with COVID-19 (Preprint)

Comparison of laboratory parameters in patients with COVID-19 (Preprint)

Використання прогностичної моделі післяопераційних ускладнень та протокол оцінки ефективності анестезії під час реконструктивних та естетичних хірургічних втручань на молочних залозах

при розробці рекомендацій стосовно вибору методу обезболення при реконструктивних та естетичних хірургічних втручаннях на молочних залозах важливою є оцінка ефективності та безпеки анестезіологічного забезпечення. Окрім впливу на центральну нервову систему, загальна анестезія чинить комплексний вплив на організм, у тому числі викликаючи зміни у різних ланках метаболізму. Метаболічні зміни, пов’язані з хірургічним стресом, включають активацію катаболічних процесів і збільшення протеолізу. Метою даної роботи було розробити модель оцінки ефективності анестезії під час реконструктивних та естетичних хірургічних втручань на молочних залозах, а також розробити відповідний протокол. В роботі проведено покрокову оцінку ефективності анестезіологічного забезпечення при реконструктивних операціях на молочних залозах. Інструментом оцінки ефективності для створенням прогностичної моделі ризику в нашій роботі був категоріальний аналіз частоти хірургічних ускладнень, а також гемодинамічних та метоболічних змін залежно від фізичного статусу пацієнтів за ASA. Для створення прогностичної моделі ризику в нашій роботі використовувався метод бінарної логістичної регресії з використанням пакету спеціального призначення SPSS. За дихотомічну перемінну прийнято вірогідність післяопераційних ускладнень у відповідності до незалежних перемінних. Серед незалежних перемінних в модель були включені такі числові параметри як вік, індекс маси тіла, вміст глюкози, креатиніну, BIS внутрішньоопераційний, внутрішньоопераційний систолічний артеріальних тиск, тривалість втручання, больовий синдром через 24 години після втручання, когнітивні порушення через 24 години після втручання. Серед категоріальних перемінних відібрані вид анестезії, клас пацієнта за ASA. У запропонованому протоколі оцінки ефективності анестезії під час реконструктивних та естетичних хірургічних втручань на молочних залозах за досягнення порогової суми коефіцієнтів з використанням шкали визначено групу ризику: якщо сума прогностичних коефіцієнтів дорівнює або нижча, ніж -19,8, ризик мінімальний; більша -19,8 і нижча 19,8, ризик невизначений; якщо дорівнює або вища, ніж 19,8, ризик високий. Чутливість протоколу склала 95,7%, специфічність — 93,5%, позитивна прогностична цінність — 91,8%, негативна прогностична цінність — 92,9%. Факторний аналіз клініко-анамнестичних, апаратних та лабораторних показників у пацієнтів, яким було проведено реконструктивні та естетичні хірургічні втручання на молочних залозах, дозволив встановити групи факторів, які визначають варіативність ознак. Розроблений протокол оцінки ефективності анестезії під час реконструктивних та естетичних хірургічних втручань на молочних залозах характеризується параметрами клінічної цінності, достатніми для застосування у анестезіологічному забезпеченні реконструктивних та естетичних хірургічних втручань на молочних залозах.

Clinical status of patients with coronary artery disease post COVID-19

The main goal of our study was to identify the activity of cardiac dysfunction based on the analysis of the main cardiological methods of research, such as ECG, echocardiography, 24-hour ECG monitoring in conjunction with laboratory parameters in patients with coronary artery disease (CAD) who underwent mild and moderate COVID-19, without signs of residual effects of lung tissue damage (fibrotization). 52 patients with coronary artery disease were examined, which were divided into 2 groups depending on the past infection in history: 1 group without COVID-19 in history (n=26) (based on history and results of SARS-CoV-2 antibody titer), 2 a group with a history of COVID-19 (n=26), confirmed by relevant documents (tests), but without oxygen therapy and steroids, in order to avoid the influence of a serious illness and drug exposure. Conclusions: dynamic monitoring of hemostasis parameters after the hospital stage in patients with CAD should be carried out in order to prevent adverse cardiovascular outcomes, even with a history of moderate and mild coronavirus infection. One of the aspects of therapeutic rehabilitation in the post-COVID period in patients with IHD is the use of vitamin D preparations.

POS-666 The Impact of a Single Hemodialysis Session on Key Echocardiographic and Laboratory Parameters in Patients with Heart Failure and Preserved Left Ventricular Ejection Fraction

Heart failure with preserved ejection fraction (HFpEF) in the patients on maintenance hemodialysis (HD) is a widely discussed problem. The aim of the study is to evaluate the impact of a single HD session on a number of hemodynamic parameters and NT-pro-BNP concentration.

Exposure to valproic acid is associated with less pulmonary infiltrates and improvements in diverse clinical outcomes and laboratory parameters in patients hospitalized with COVID-19.

BackgroundValproic acid (VPA) has shown beneficial effects in vitro against SARS-CoV-2 infection, but no study has analyzed its efficacy in the clinical setting.MethodsThis multicenter, retrospective study included 165 adult patients receiving VPA at the time of admission to hospital, and 330 controls matched for sex, age and date of admission. A number of clinical, outcome and laboratory parameters were recorded to evaluate differences between the two groups. Four major clinical endpoints were considered: development of lung infiltrates, in-hospital respiratory worsening, ICU admissions and death.ResultsVPA-treated patients had higher lymphocyte (P<0.0001) and monocyte (P = 0.0002) counts, and lower levels of diverse inflammatory parameters, including a composite biochemical severity score (P = 0.016). VPA patients had shorter duration of symptoms (P<0.0001), were more commonly asymptomatic (P = 0.016), and developed less commonly lung infiltrates (65.8%/88.2%, P<0.0001), respiratory worsening (20.6%/30.6%, P = 0.019) and ICU admissions (6.1%/13.0%, P = 0.018). There was no difference in survival (84.8%/88.8%, P = 0.2), although death was more commonly related to non-COVID-19 causes in the VPA group (36.0%/10.8%, P = 0.017). The cumulative hazard for developing adverse clinical endpoints was higher in controls than in the VPA group for infiltrates (P<0.0001), respiratory worsening (P<0.0001), and ICU admissions (P = 0.001), but not for death (0.6). Multivariate analysis revealed that VPA treatment was independently protective for the development of the first three clinical endpoints (P = 0.0002, P = 0.03, and P = 0.025, respectively), but not for death (P = 0.2).ConclusionsVPA-treated patients seem to develop less serious COVID-19 than control patients, according to diverse clinical endpoints and laboratory markers.

Routine laboratory parameters in patients with necrotizing pancreatitis by the time of operative pancreatic debridement: Food for thought.

BACKGROUNDTiming of invasive intervention such as operative pancreatic debridement (OPD) in patients with acute necrotizing pancreatitis (ANP) is linked to the degree of encapsulation in necrotic collections and controlled inflammation. Additional markers of these processes might assist decision-making on the timing of surgical intervention. In our opinion, it is logical to search for such markers among routine laboratory parameters traditionally used in ANP patients, considering simplicity and cost-efficacy of routine laboratory methodologies.AIMTo evaluate laboratory variables in ANP patients in the preoperative period for the purpose of their use in the timing of surgery.METHODSA retrospective analysis of routine laboratory parameters in 53 ANP patients undergoing OPD between 2017 and 2020 was performed. Dynamic changes of routine hematological and biochemical indices were examined in the preoperative period. Patients were divided into survivors and non-survivors. Survivors were divided into subgroups with short and long post-surgery length of stay (LOS) in hospital. Correlation analysis was used to evaluate association of laboratory variables with LOS. Logistic regression was used to assess risk factors for patient mortality.RESULTSSeven patients (15%) with severe acute pancreatitis (SAP) and 46 patients (85%) with moderately SAP (MSAP) were included in the study. Median age of participants was 43.2 years; 33 (62.3%) were male. Pancreatitis etiology included biliary (15%), alcohol (80%), and idiopathic/other (5%). Median time from diagnosis to OPD was ≥ 4 wk. Median postoperative LOS was at the average of 53 d. Mortality was 19%. Progressive increase of platelet count in preoperative period was associated with shortened LOS. Increased aspartate aminotransferase and direct bilirubin (DB) levels the day before the OPD along with weak progressive decrease of DB in preoperative period were reliable predictors for ANP patient mortality.CONCLUSIONMultifactorial analysis of dynamic changes of routine laboratory variables can be useful for a person-tailored timing of surgical intervention in ANP patients.

Antiinflammatory potential of nano-curcumin as an alternative therapeutic agent for the treatment of mild-to-moderate hospitalized COVID-19 patients in a placebo-controlled clinical trial.

The present study conducted a placebo-controlled clinical trial to evaluate the impact of nano-curcumin on the inflammatory cytokines in mild-to-moderate hospitalized COVID-19 patients. A total of 60 COVID-19 patients were randomly divided into nano-curcumin and control groups, and then they received 240 mg/day nano-curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real-time PCR and ELISA techniques were implicated in assessing the mRNA expression of IFN-γ, IL-1β, IL-6, MCP-1, and TNF-α and the serum levels of IL-1β, IL-6, and TNF-α inflammatory mediators, respectively. Although the clinical manifestations and laboratory parameters improved via the nano-curcumin treatment, the mRNA expression of IFN-γ (p= 0.006) and TNF-α (p= 0.04) were significantly reduced. Besides, a considerable difference was observed between the nano-curcumin and control groups in the expression of IFN-γ (p= 0.001), IL-1β (p= 0.0002), and IL-6 (p= 0.008). In addition, there was a significant difference between the nano-curcumin and control groups in the serum levels of IL-1β (p= 0.042). The evidence demonstrated that nano-curcumin could be implicated as a complementary medication to act as an antiinflammatory agent and inhibit inflammatory complications.

Показники лейкоцитарної та тромбоцитарної ланки крові в динаміці госпітального періоду у хворих з гострим інфарктом міокарда: можливість використання для оцінювання ризику розвитку віддалених ускладнень

The aim – to create a new method of assessing the development of long-term complications in STEMI patients by studying blood cell composition and its adaptation to practical application in general clinical practice.Materials and methods. The study was involved 148 patients with acute myocardial infarction (AMI) who was admitted from January 2014 to June 2020 to the intensive care unit. Some patients were evaluated retrospectively and were in group 1 (n=92). Group 2 – 56 patients, who were studied prospectively. The groups of patients did not differ in clinical and anamnestic characteristics and treatment. The study provided an annual observation period. The endpoint in group 1 was: death, stroke, exacerbation of coronary heart disease – including the need for revascularization, the developement or decompensation for heart failure, which led to hospitalization (in addition, group 2 was analyzed for onset of cardiac death).Results and discussion. There complex indicators were built, based on the analysis of the clinical profile and dynamics of laboratory parameters in patients with the onset of the endpoint – a modified leukocyte index (mLI), which contains the values ​​of the number and percentage of granulocytes, lymphocytes and monocytes on days 1, 3 and 10 of STEMI and leukocyte-platelet index (mLPI), which additionally includes indicators of platelet inhomogeneity in size (PDWc and P-LCR). These indices with their limit values ​​(mLI &gt; 140 units and mLPI &gt; 242 units) were more informative in predicting distant cardiovascular events than other laboratory markers (including neutrophil-leukocyte ratio, NLR). In a prospective study branch (group 2), the mLI and mLPI indicators also turned out to be more informative than other markers (in particular, the NLR indicator) in determining the propensity to occur as a combined endpoint (area under the curve 0.71 for both; p&lt;0.0001), so and death (areas under the curve 0.78 and 0.84, respectively; p&lt;0.0001). Based on the data obtained, a computer algorithm has been created that simplifies the risk assessment in AMI patients using the developed indicators.Conclusions. Created leukocyte and leukocyte-platelet indices are highly informative in predicting the risk of complications in patients within a year after AMI.

Mid-regional pro-adrenomedullin as apredictor of in-hospital mortality in adult patients with COVID-19: asingle-centre prospective study.

To determine the predictive value of mid-regional pro-adrenomedullin (MR-proADM) compared to routine clinical and laboratory parameters in patients with COVID-19. A total of 135 adult patients hospitalized with COVID-19 were included in a prospective single-centre study. In addition to routine parameters, the levels of MR-proADM in blood plasma were measured on the day of hospitalization. The patients were divided into 2 groups: those who survived and were discharged (n = 115, 85%) and those who did not survive (n = 20, 15%). Data are presented as median and interquartile range. The non-survivors had a statistically significantly greater age (73.4 [63.5-84.8] vs. 62.2 [50.3-71.4] years, P = 0.001), a lower level of haemoglobin oxygen saturation (91 [87-92] vs. 92 [92-93]%, P &lt; 0.001), lower lymphocyte level (13 [7-30] vs. 21 [15-27]%, P = 0.03), higher lactate dehydrogenase (338 [273-480] vs. 280 [233-383] EU L-1, P = 0.04) and aspartate aminotransferase levels (49 [28-72] vs. 33 [23-47] EU L-1, P = 0.03), a higher National Early Warning (NEWS) score (7 [7- 8] vs. 6 [5-7] points, P &lt; 0.001), and higher procalcitonin (0.16 [0.11-0.32] vs. 0.1 [0.07-0.18] ng mL-1, P = 0.006) and MR-proADM levels (1.288 [0.886-1.847] vs. 0.769 [0.6-0.955] nmol L-1, P &lt; 0.001). MR-proADM had the highest predictive value for death during hospital stay (cut-off: 0.895 nmol L-1, AUC ROC 0.78 [95% CI: 0.66-0.90], sensitivity 75%, specificity 69%, OR 6.58 [95% CI: 2.22-19.51]). Compared with other indicators, MR-proADM has the highest predictive value for in-hospital mortality in patients with COVID-19.

CORRELATIONS OF BODY MASS INDEX WITH CLINICAL AND LABORATORY INDICATORS IN PATIENTS WITH ESSENTIAL ARTERIAL HYPERTENSION DEPENDING ON AGT (RS699) GENE POLYMORPHISM

The aim. To analyze the correlations between body mass index (BMI) and clinical and laboratory parameters in patients with essential hypertension (EAH) depending on AGT gene (rs699) polymorphism. Materials and methods. The case-control study involved 72 patients with stage II EAH, 1–3 degrees of blood pressure (BP), high and very high cardiovascular risk (CVD) (29.16% (21) men, 70.84% ​​(51) women). The mean age of patients was 59.87 ± 7.98 y.o. The control group consisted of 48 healthy individuals comparable in age and gender. Polymorphism of the AGT gene (rs699) was detected by polymerase chain reaction. BMI was defined as the ratio of weight to square of height (kg/m2). Results. The correlation matrix showed a direct relationship between BMI and anthropometric parameters of waist circumference (WC), hip circumference (HC) and WC/HC ratio (r = 0.70–0.81; p ≤ 0.022), vitamin D level (r = 0,65; p = 0.043) and the reverse with the level of ionized Ca2+ in the blood (r = -0.71; p = 0.02) among TT-genotype carriers; in TC-genotype carriers BMI was directly related to WC and HC (r = 0.68; p &lt; 0.001 and r = 0.84; p &lt; 0.001), as well as the level of parathyroid hormone (PTH) (r = 0,43; p = 0.004), with a negative correlation with the level of vitamin D (r = -0.38; p = 0.011); in CC-genotype carriers, a direct link was found between BMI and WC and HC (r = 0.73; p &lt; 0.001 and r = 0.78; p &lt; 0.001). Conclusions. Thus, the correlation matrix showed that BMI directly depended on the anthropometric parameters of WC, HC and/or WC/HC (r = 0.68-0.84; p ≤ 0.022-0.001) regardless of the AGT gene genotypes (r = 0,52-0.86; p ≤ 0.02-0.001) and inversely correlated with the ionized Ca2+ level in the blood (r = -0.71; p = 0.02), but only in TT-genotype carriers of the AGT gene (rs699).

Correlation between imaging morphological findings and laboratory biomarkers in patients with retinal vein occlusion

AbstractPurposeTo investigate the potential association between peripheral blood biomarkers and morphological characteristics of retinal imaging in patients with retinal vein occlusion (RVO).MethodsParticipants in this cross‐sectional observational study were 65 consecutive patients (65 eyes) with treatment naïve RVO, who underwent spectral‐domain optical coherence tomography (SD‐OCT) and fundus fluorescein angiography (FFA). In addition, peripheral blood samples were taken to evaluate full blood count and biochemical parameters. Correlation between imaging characteristics and laboratory parameters was examined.ResultsEyes with subretinal fluid presented significantly higher neutrophil‐to‐lymphocyte ratio (p = 0.028). Hyperreflective foci on SD‐OCT were found to be associated with higher triglycerides (p = 0.024). Presence of cysts on SD‐OCT was associated with significantly higher triglycerides (p = 0.010). Central subfield thickness (CST) higher than 464 μm was associated with higher lymphocyte count (p = 0.016) and higher urea (p = 0.015). No significant associations were found between laboratory parameters and intraretinal fluid, ellipsoid zone and external limiting membrane condition, epiretinal membrane and macular ischemia.ConclusionsSpecific imaging morphological characteristics were found to be associated with laboratory parameters in patients with RVO. These findings may scrutinize the pathophysiology of RVO and its correlation with the development of specific clinical signs, while they could guide individualized treatment.

Real-life antimicrobial therapy in hospitalized patients with COVID-19 (preliminary results and recommendations)

Objective. Development of local clinical protocols for antibacterial therapy of COVID-19-associated bacterial pneumonia in the therapeutic department of the city clinical hospital based on an analysis of the treatment process in patients with COVID-19-associated pneumonia. Materials and Methods. A retrospective analysis of 1382 cases of hospitalization in the therapeutic department of patients with COVID-19-associated pneumonia for the period from 2020 to 2021 was carried out. The structure of etiotropic therapy, the frequency and timing of microbiological studies of the biomaterial, the manifestations of the main markers of bacterial infection during dynamic monitoring of clinical and laboratory parameters in patients prescribed antibiotic therapy, as well as statistics of the stay of patients in the therapeutic department of the hospital were assessed. Based on the results obtained in the course of microbiological studies, an assessment was made of the microbial landscape of the lower respiratory tract of patients with an analysis of the sensitivity of strains of the leading microflora to a wide range of antibiotics. Results. The study found that the dominant flora in COVID-19-associated pneumonia in hospitalized patients was gram-negative bacteria – K. pneumoniae, P. aeruginosa and A. baumannii – their proportion was more than 50%. Among K. pneumoniae strains, 89.4% were ESBL producers, 63.5% of the strains were resistant to carbapenems, which with a high probability allows them to be considered carbapenemaseproducing strains. Among the strains of P. aeruginosa, the proportion of strains resistant to carbapenems and with a high degree of probability being strains – producers of carbapenemase was 41.1%. Among strains of Acinetobacter spp. these were 76.4%, and associated resistance to fluoroquinolones and aminoglycosides was also demonstrated. Gram-positive microorganisms were found in 34.3% of cases and were mainly represented by strains of S. aureus (74.9%), only 26.4% of strains of this pathogen were methicillin-resistant. Conclusions. Microbiological monitoring conducted in 2020–2021 revealed the presence, among the pathogens of viral-bacterial pneumonia, at an early stage of hospitalization, a significant proportion of gram-negative bacteria with resistance of the MDR and XDR types. Based on the obtained microbiological data, starting empirical schemes for antibacterial therapy of secondary viral and bacterial pneumonia, which complicated the course of a new coronavirus infection COVID-19 caused by the SARS-CoV-2 virus, were developed and proposed.