Laboratory Experimental Method Research Articles

The Effect of the Ratio of Fly Ash to Alkaline Activator and the Molarity of NaOH on the Mechanical Properties of Fly Ash-Based Aggregates

The global demand for aggregates has escalated, leading to a decline in the availability of quality natural aggregates. In response, the cold bond pelletization (CBP) process emerged in the early 2000s as a novel approach to artificial aggregate production, utilizing dry powdered fly ash. This method involves agglomerating fly ash particles at room temperature in an inclined rotating pan to form pellets. However, the abstract lacks clarity in elucidating the significance of this approach. Our study investigates the mechanical properties of aggregates produced through the CBP method via experimental laboratory methods, focusing on variations in the ratio of fly ash to alkaline activator (FA/AA) and the molarity of Sodium Hydroxide (NaOH). Notably, we found that an effective molarity of 15 resulted in the lowest Aggregate Impact Value (AIV) percentage, indicating improved mechanical properties. Furthermore, we observed fluctuations in AIV values across different FA/AA ratios and NaOH molarities, suggesting nuanced effects on aggregate quality. Our findings underscore the importance of optimizing parameters in the CBP process for enhanced aggregate performance.

FORMULATION AND EVALUATION OF SACHA INCHI OIL LIP BALM PREPARATIONS WITH CONCENTRATION VARIATIONS OF CERA ALBA AND LANOLIN AS LIP BALM

Lip balm is used on lips that require protection, such as when humidity is low, to prevent the evaporation of water and epithelial cells on the lip mucosa. The active substance used in making this lip balm is Sancha inchi oil, which is a cold-pressed oil from the seeds of Plukenetia volubilista. This oil contains omega 3 (40–50%) and omega 6 (30–40%), as well as lower oleic acid (10%) than olive oil. Natural antioxidants, including tocopherols and polyphenols, have also been found in Sacha Inchi oil. Sacha inchi oil has a fatty acid ratio and phytochemical composition, it can be used as a cosmetic ingredient as an emollient and skin protector. An experimental laboratory method was used for this study. The research was carried out by preparing lip balm from various ingredients containing the active ingredient Sacha inchi oil (Plukenetia volubilis L) at a concentration of 15%, as well as varying concentrations of cera alba (range 5-25%) and lanolin (range 2-10%) as bases and additional ingredients for preparations, whose optimization had been determined using the simplex lattice design method. The aim of this study was to identify the lip balm formulation from Sacha inchi oil and identify the effect of changes in the concentration of cera alba and lanolin based on the results of the preparation evaluation. The results of this test show that sacha inchi oil can be used as an active lip-moisturizing agent in lip balm preparations ...

Optimization of Complete Blood Count Results with Variations in Specimen Handling and Whole Blood Secondary Homogenization Techniques

Specimen homogenization and the addition of anticoagulants are pre-analytical steps that influence the accuracy of the test results. Complete blood count (CBC) is a screening test that supports disease diagnosis and aids in determining the appropriate therapy for patients. Inadequate specimens are inappropriate for subsequent testing, as they can lead to inaccurate results in the CBC examination. This study aims to determine the optimization of complete blood count results with variations in specimen handling and secondary homogenization techniques for whole blood. The type of research used is quantitative analysis using laboratory experimental methods. The study was conducted in May 2024 in the Clinical Pathology Laboratory of the Diploma-4 Medical Laboratory Technology Study Program, Faculty of Health Sciences, Muhammadiyah University of Sidoarjo, using 48 blood samples. The results of the MANOVA test indicate that the type of anticoagulant has a significant effect on the erythrocyte count (p=0.041) but does not have a significant impact on the leukocyte count (p=0.844) and platelet count (p=0.920). Meanwhile, the homogenization technique does not significantly affect the erythrocyte count (p=0.959), leukocyte count (p=0.991), or platelet count (p=0.867). This study concludes that the secondary homogenization technique has no significant effect. In contrast, the type of anticoagulant significantly impacts the results of the Complete Blood Count (CBC) test. This research suggests collecting whole blood specimens using Ethylene Diamine Tetra Acetate (EDTA) vacutainers as the anticoagulant and applying a secondary homogenization technique before performing the CBC analysis.

Antioxidant activity of Eucheuma cottonii Seaweed Extracts from Central Mawasangka Waters

Eucheuma cottonii is one type of seaweed that has the potential to be an antioxidant. This seaweed grows and is distributed in various waters of Indonesia, especially in Southeast Sulawesi. However, information regarding the antioxidant activity and the compound content of E. cottonii in this area is still limited. Therefore, this research aims to determine the compound and antioxidant activity profile in E. cottonii. The study used laboratory experimental methods. The research stages were preparation (seaweed sampling), extraction, qualitative phytochemical screening, and antioxidant assay. The initial identification of compounds using qualitative phytochemical screening methods with three extraction solvents: ethanol, ethyl acetate, and n-hexane. Meanwhile, the antioxidant test employed the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results of qualitative phytochemical screening revealed that the seaweed extract of E. cottonii contains saponins, tannins, and terpenoids. The antioxidant assay using the DPPH method indicated that among the three extraction solvents used, ethanol extract exhibited a very strong antioxidant activity (with an IC50 of 30,036 ppm), compared to ethyl acetate extract (IC50 of 182,179 ppm) and n-hexane extract (IC50 of 641,454 ppm). This study concluded that E. cottonii seaweed has the potential to be developed as a source of natural antioxidants or raw material for the discovery of antioxidant medicines in the future.

Effect of Saga Leaf Extract (Abrus precatorius Linn) in Inhibiting Enterococcus faecalis Bacteria Growth as an Alternative Root Canal Irrigation Material

Abstract Objective This study aims to examine the antibacterial effect of saga leaf extract on Enterococcus faecalis bacteria, which causes root canal treatment failure. Materials and Methods This research was conducted using a laboratory experimental method with saga leaf extract at 3.125, 6.25, 12.5, 25, 50, and 100% concentrations. Two percent chlorhexidine acts as the positive control, and 100% dimethyl sulfoxide as the negative control. The diameter of the inhibition zones was measured using the well diffusion test method. Statistical Analysis Data from the measurement of inhibition zone diameter were obtained and tested statistically using the normality test (Shapiro–Wilk), homogeneity test (Levene), parametric test (one-way analysis of variance), and further test (post hoc test). Results The largest inhibition zone diameter observed in this study was 9.46 mm at 100% concentration; however, it was not bigger than the positive control, which was measured at 16.55 mm. The research data were analyzed based on the classification of Davis and Stout inhibition zones. Conclusion This study concludes that saga leaf extract has an antibacterial effect on the growth of E. faecalis bacteria.

The effect of nano propolis on surface color changes of nanohybrid composite resins

Background: Nanohybrid composite resins have many advantages, but one disadvantage is their absorbent nature which can lead to discoloration. Phenols and flavonoids in nano propolis cause diffusion and affect the matrix and pigments of nanohybrid composite resins. The accumulation of reduction-oxidation reactions causes new compounds of nanohybrid composite resins to occur so that they have different colors. Objective: The study was conducted to determine the changes in surface color of nanohybrid composite resin after immersion in nano propolis at 5% concentration. Methods: Research experimental laboratory method with pre and post test design was applied to 25 nanohybrid composite resin fruit samples of size (5x5x1.5) mm embedded in bovine incisive, then immersed in 5% nano propolis and 25 ml of distilled water at 37oC for five days. The color change (ΔE) was measured with a DSLR camera and processed with Adobe Lightroom Classic software in CIE L*a*b* format. Results: The anova test on ΔE in the data showed the p-value of sphericity assumed was smaller than 0.05. There is a difference in the average measurement results on the first day to the fifth day in the treatment group. Independent t-test of control and treatment data showed a significant difference in measurement results. The results of the post hoc test with the Tukey method showed that the treatment groups at 4x24 hours and 5x24 hours did not have significant differences. P-value at 4x24 hours and 5x24 hours was 0.957. Conclusion: There was a change in the surface color of the nanohybrid composite resin after being immersed in a nano-solution of propolis with a concentration of 5%.

Performance of The White-Rumped Shama (Copysychus malabaricus) Reproduction by Providing Eurycoma longifolia at The Captivity

This research aims to determine the reproductive capacity of the White-Rumped Shama provided by Eurycoma longifolia in terms of duration of couple introduction or adjustment period, duration of mating period until laying eggs, number of eggs, number of hatched eggs, and number of survived baby animals until 5 days of living. The laboratory experimental method is performed in the White-Rumped Shama captivity in Bogor. Before the treatment, the birds are matched in each bird cage. The White-Rumped Shamas are divided randomly into 2 groups, each of which consists of 5 pairs (5 repetitions). The first group (K1) as a control, male magpie parents were given the treatment of drinking distilled water after fasting for one hour every morning, Group 2 (K2) as the Eurycoma longifolia treatment group, male White-Rumped Shama parents were given the optimal dose of Eurycoma longifolia, 9 mg/100 of the body weight every morning for 5 days which was converted from the results of research on the treatment of giving Eurycoma longifolia to white rats. Statistical analysis of two tailed T test, Levene's test on data between the control group (K1) and the Eurycoma longifolia group (K2) showed that; Behavior: Jumping, flying, perching on the perchs, approaching the female, chirping, singing, crouching while near to the female while making a distinctive chirp, eating and drinking, duration of time until laying eggs in the mating cage (days), number of eggs in the nest (items ). The number of hatched eggs from those hatched (grains), the number of offspring that survived to the age of 5 days (tails), there were no significant differences between the control group (K1) and the Eurycoma longifolia treatment group (K2).

Uji Daya Hambat Ekstrak Lidah Buaya (Aloe barbadensis miller) terhadap Pertumbuhan Bakteri Propionibacterium Acnes

Aloe vera (Aloe barbadensis miller) contains antibacterial compounds such as tannins and saponins, which have the potential to combat Propionibacterium acnes, the bacteria responsible for acne. This study aims to evaluate the inhibitory effect of aloe vera extract on P. acnes through a laboratory experimental method. The extract was obtained through maceration using various concentrations: 20%, 40%, 60%, 80%, and 100%. Testing was conducted in triplicate, with clindamycin as the positive control and distilled water as the negative control. The well diffusion method was used for testing, followed by analysis with a One-Way ANOVA test. Results showed p = 0.000, indicating that aloe vera extract significantly inhibits P. acnes. Post hoc LSD analysis revealed significant differences between concentrations, except between clindamycin and the 100% concentration (p > 0.05). The highest mean inhibition zone was found at the 100% concentration (16.6 mm) and the lowest at 20% (9.3 mm). This study suggests that aloe vera extract has potential as a natural antibacterial agent for acne treatment, providing an alternative to reduce synthetic antibiotic use and the risk of bacterial resistance.

Effect of kopyor coconut water on early-onset preeclampsia-like impairments in rats induced by L-nitro-arginine methyl ester

Preeclampsia (PE) is a severe pregnancy disorder posing significant health risks to both the mother and the fetus without available preventive measures or treatments. More specifically, the main pathological feature of early-onset PE (EO-PE) is the incomplete transformation of the spiral artery. Meanwhile, aspirin has proven effective for the treatment and prevention of PE. Previous studies indicated a relationship between the phytochemical compound of kopyor coconut water (KCW) and nutritional treatment for PE. Therefore, the objective of this study was to determine the efficacy of KCW as a potential preventive treatment for PE. An experimental laboratory method was used with pre-test and post-test control group designs. The samples comprised 35 pregnant Wistar rats, divided into five groups with seven members each. Rats were then randomized into control and groups exposed to L-NAME, L-NAME and aspirin, L-NAME and KCW 2 ml/200 gBW, as well as L-Name and KCW 2 ml/200 gBW for GD4 to 19. The results showed that after administering L-NAME to induce EO-PE, mean arterial pressure (MAP), proteinuria, placental hypoxia, oxidative stress, and endothelial dysfunction decreased due to KCW nutritional treatment. Specifically, KCW nutrition prevented the uterine spiral artery from expanding and reduced the number of neutrophils. The decreased survival rate caused by L-NAME-induced PE was reversed by providing KCW nutrition. Moreover, results indicated that KCW was a potential alternative for the prevention and treatment of EO-PE even at doses of 2 or 3 ml/200 gBW, offering insights for the community and clinical practitioners in treating PE as a therapeutic option.

FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF RAMBUTAN HONEY TOOTHPASTE AGAINST Streptococcus mutans

Dental caries and periodontal diseases are the most common diseases affecting the oral cavity. This can be prevented by maintaining oral hygiene. Toothpaste plays an important role in the reduction of plaque bacteria and inflammatory conditions. Rambutan honey has potential as a natural ingredient to make toothpaste with antibacterial and antioxidant properties owing to the presence of hydrogen peroxide and catalase enzymes, pH, osmolarity, and flavonoid content. The purpose of this study was to obtain a rambutan honey toothpaste formulation that effectively inhibits Streptococcus mutans growth. The laboratory experimental research method was used to combine CaCO3, glycerin, Na CMC, SLS, sodium benzoate, saccharin, menthol, and aquadest as the toothpaste base, which served as the control group. Subsequently, natural ingredients of rambutan honey at concentrations of 20%, 40%, and 60% were added to the toothpaste base to create rambutan honey toothpaste. The formulation of the rambutan honey toothpaste was followed by an antibacterial activity test using the Kirby Bauer method of disc diffusion, where the inhibition zones formed on Mueller Hinton Agar (MHA) medium were calculated. Data were analyzed using one-way and post hoc Tukey’s tests (p<0.05). The results showed that the formulation of rambutan honey toothpaste with a concentration of 60% had the greatest inhibition with 13.88±0.13 mm with a significant difference in each group (p = 0.000), so it can be concluded that rambutan honey toothpaste can inhibit the formation of Streptococcus mutans. Keywords: antibacterial, formulation, rambutan honey, toothpaste, Streptococcus mutans

Development of methods for generating examples that train a neural network to detect and classify interference in the structure of a useful signal

The main purpose of the information transmission system is to transfer useful information between its users reliably and accurately. At the same time, it would be advisable to supplement the functionality of the information transmission system with the ability to analyze interference in the communication channel during the transmission of a message. In the case of analysis of damage to the useful signal, which may occur due to interference of various types in the propagation medium, the information transmission system can use the results of such analysis to improve noise immunity, to collect statistics, to control electromagnetic compatibility. It is known that optimal processing of noise-like signals is carried out using a correlator or a matched filter, but these classical devices might be complemented with a neural network, and then in addition to determining the correlation function, it is the neural network that is able to analyze a signal damaged by interference, as shown by the authors in their previous works. In this case, for the correct training of a neural network, samples of a useful signal damaged by various types of jamming are needed. This article discusses methods for the formation of training images for a neural network designed to determine the period of pulse interference acting on an M-sequence as a known signal. Two techniques are suggested. They are digital modelling and experimental (laboratory) method. The first one is based on the inverting some elements (chips) of the original M-sequence located in certain segments whose lengths are random. The inversion is performed by the program written in C++ language. The second approach involves a special installation that consists of a personal computer, an Arduino microcontroller, a useful signal transmitter, a receiver and a periodic interference generator. The objective of each method is to obtain a matrix of damaged chips in a chain of M-sequences. Such matrix was called an “error profile” by the authors. The characteristics of the error profile obtained for certain interference parameters (jamming pulse period and duration) according to suggested methods can be used as training data for the neural network designed for jamming classification.

Physicochemical And Organoleptic Quality Testing Of Millet Microgreen Juice (Panicum Miliaceum L.) On Different Milet Types And Plant Age

This research has taken place using experimental laboratory methods of quantitative analysis. The variables used include varieties consisting of yellow millet and red millet, and plant life ranging from 6 days, 9 days, 12 days, and 15 days. The data obtained was analyzed based on parametric statistics using ANSIRA, using statistical product and service solution (SPSS) with version 24 and determining best treatment using effectiveness tests and determining antioxidant levels of best treatment. Different types and ages of plants have very real effects on reduced sugars, phenols, vitamin C, flavonoids and total soluble solids. Significant treatment between different plant types and ages resulted in a 6-day-old ryellow millet (MG1) being the best treatment with a result value (NH) of 0.73 with the parameter criterion reduction sugar level of 1.48%, total phenol content of 27.26 mg GAE/100mg, vitamin C 2.05 mg/100 mg, total flavonoid content of 4.98 mg QE/100 Mg, TPT 11.36 mg/L, color 4.81 (neutral), flavor 5.16 (few likes) and aroma 5.4 (few likes).

Isolasi Dan Identifikasi Bakteri Proteolitik Dari Tanah Mangrove Di Pantai Pandaratan Kecamatan Sarudik Kabupaten Tapanuli Tengah

Proteolytic bacteria are bacteria that can degrade proteins by producing extracellular protease enzymes. Proteolytic bacteria can be found in mangrove soil. This is because the dry weight of mangrove leaf litter contains 61% protein. The purpose of this study was to determine the presence of proteolytic bacterial isolates from mangrove soil in Pandaratan Beach, Sarudik District, Central Tapanuli Regency and to characterize them. This study used a laboratory experimental method which was analyzed descriptively. Soil sampling was carried out at two location points I (sandy) and location II (muddy) which were isolated using SMA media with the streak plate method. Characterization includes macroscopic and microscopic observations as well as biochemical tests. The results of the study found that there were 15 isolates of proteolytic bacteria isolated from the mangrove soil of Pandaratan Beach, Central Tapanuli. The highest activity test of the proteolytic index (IP) was obtained 0.82 mm by the Micrococcus (L1) and 0.09 mm lowest by Brevibacillus (L2). A total of 11 genera of proteolytic bacteria were found, including Curtobacterium (P1 and L7), Bacillus (P2, P4, and P7), Enterococcus (P3), Brevibacillus (P5 and L2), Carnobacterium (P6), Paenibacillus (P8), Micrococcus (L1), Vagococcus (L3), Marinococcus (L4), Halobacillus (L5), Sporosarcina (L6).

Peningkatan Pengetahuan Siswa Sekolah Dasar tentang Bahan Tambahan Pangan Berbahaya dengan Metode Eksperimen Laboratorium

Food Additives in general are to increase the nutritional value of food, improve the aesthetic and sensory value of food and extend the shelf life of food. There is abuse of food additives which can endanger consumers, such as adding borax and formaldehyde to food. This community service activity is an effort to increase elementary school students' knowledge of dangerous BTP using laboratory experimental methods. The results show that there is an increase in students' knowledge of dangerous food additives.

Analysis of precursor response characteristics and time-frequency vibration parameters of rock failure: a laboratory experimental method

ABSTRACT To explore the vibration characteristics of rock failure precursors, a vibration optical force combined monitoring method was adopted. In this study, quasi-sandstone and granite samples were selected for uniaxial loading experiments to monitor the surface crack propagation and vibration. The variations in acceleration, displacement, energy, and waveform characteristics of quasi-sandstone and granite were studied from the perspective of the time domain. Additionally, a warning parameter SKE (Skewness, Kurtosis and Energy) based on waveform characteristics and energy was proposed. From the perspective of time-frequency analysis, a warning parameter CFEFD (Center Frequency Energy and Frequency Difference) is proposed by comprehensively utilising short-time Fourier analysis and statistical methods. The prediction effectiveness of both parameters was evaluated. It was observed that the vibration acceleration, energy, and displacement of rocks can all reflect the propagation of rock cracks. Prior to reaching the peak stress, the vibration acceleration and energy of rocks undergo sudden changes. The development of rock fractures releases energy, leading to high-energy frequency diffusion. Before reaching the peak stress, the range and energy level of high-energy frequency diffusion show significant improvement. Finally, it was found that both the SKE and CFEFD warning indicators can provide a certain degree of warning effect.

The effect of changing vertical irradiation angle of periapical radiography bisecting technique on the length dimension of mandibular teeth

Objectives: This research is aimed to determine the effect of changing the vertical irradiation angle with a change in angle of +10°, +5°, -5°, -10° in bisecting technique periapical radiography on the length dimension of mandibular teeth. Materials and Methods: This study used a laboratory experimental method with a post test only control group design. The samples used in this study were teeth I1, I2, C, P1, P2, M1, and M2 region 4. All teeth were treated by changing the angle from the standard angle recommended by White and Pharoah with changes of +10°, +5°, -5°, and -10° in each tooth using a Morita Veraview iX 70 Kv, 7mA dental X-ray unit. Results: The results showed that the angle change of -10°, -5° causes foreshortening and +10°, +5° causes elongation. Based on the results of the Saphiro-Wilk and Kruskal-Wallis test, it is known that there are significant differences in the dimensions of tooth length at +10°, +5°, -5°, -10° against changes in the angle 0° or angulation form White and Pharoah (p < 0.05) . Conclusion: There is a significant effect on the change of vertical irradiation angle with angle changes of +10°, +5°, -5°, -10° in bisecting technique periapical radiography on the length dimension of mandibular teeth.

Antibacterial Activity Test of Kersen Fruit Kombucha ( Muntinga Calabura L. ) To Bacteria Staphylococcus Aureus and Escherichia Coli By In Vitro

Bacterial infections of Staphylococcus aureus and Escherichia coli cause diarrhea. Kersen fruit can be used as the basis of kombucha, which has antibacterial benefits. This experimental activity aims to determine the antibacterial activity against Escherichia coli and Staphylococcus aureus. This study used a laboratory experimental method to test the antibacterial activity of Kersen Fruit Kombucha (KFK) at concentrations of 15%, 25%, and 35%, as well as positive control of cotrimoxazole and normal control of aqua pro injection. Antibacterial activity was measured in vitro using the disc diffusion method to compare affected and unaffected areas. The results of the study on Escherichia coli and Staphylococcus aureus bacteria showed that the diameter of the inhibition zone in 15% KFK was 7.07-8.63 mm, 25% KFK showed an inhibition zone diameter of 8.11-9.57 mm; and 35% KFK inhibition zone diameter was 12.07-13.07 mm; for the positive control inhibition zone diameter was 13.07-14.72 mm. 35% KFK has a larger inhibition zone diameter than 15% and 25% KFK. Based on the Kruskal-Wallis statistical test, there was a significant difference. This study aims to determine the antibacterial activity of Kersen fruit kombucha against Staphylococcus aureus and Escherichia coli by measuring the diameter of the inhibition zone

Analysis of Environmentally Friendly Concrete (Green Concrete) Using Coal Fly Ash as a Substitute Material for Portland Cement in Road Pavement

The development of technology in the field of construction, especially in concrete technology, is now becoming more rapid, which requires concrete materials that can adapt to the times. As well as for energy savings from normal concrete materials from a mixture of fine, coarse, cement and water can now be added to other materials. In this era, the use of cement for concrete making materials is very identical, therefore to reduce the use of cement, it is necessary to mix other materials. The research method that researchers use is the experimental-laboratory method by making test objects in the form of concrete cylinders with a size of 15x30 cm. Analysis of concrete testing using Fly Ash mixtures in this study there are several variations of fly ash mixtures, namely fly ash 50%, 60%, and 70%. In testing several Fly Ash mixtures produced a compressive strength value of 50% Fly Ash percentage and 2% Accelerator of 161.630 kg/cm2, 60% Fly Ash and 2% Accelerator of 154.189 kg/cm2, 70% Fly Ash and 2% Accelerator of 92.670 kg/cm2. In the results of this study with several variations of fly ash mixtures, the highest compressive strength value was obtained at a percentage of Fly Ash 50% and Accelerator 2%, which amounted to 161.630 kg/cm2 with the first variation test specimen sample at the age of 28 days of concrete.

The Duracy of Fermentation Effect on the Chemical Quality of Kimchi Krai (Cucumis sativus L.) as Probiotics

This study aims to describe the effect of fermentation duration on the chemical quality of kimchi krai as a food containing probiotics, including total LAB, total titratable acid, pH, antioxidant activity, and level of liking. This research used laboratory experimental methods, and data analysis was done descriptively. Several stages were carried out, including sample making, sample preparation, sample extraction, total LAB test, pH test, total titratable acid test, antioxidant activity test with DPPH method to obtain IC50 value, and organoleptic test. This study used fermentation durations of 8 hours, 16 hours, and 24 hours. The best chemical quality of kimchi krai was obtained in 24-hour fermentation with a total LAB of 6.1x107, pH value decreased from pH 4.75 in 8-hour fermentation to 3.85 in 24-hour fermentation, while for total titratable acid with the highest value of 0.62%, the strongest antioxidant activity with IC50 429.86 ppm. Based on the results, the very weak category includes antioxidant activity in kimchi krai (Cucumis Sativus L.). However, the longer the fermentation process of kimchi krai (Cucumis Sativus L.), the greater the ability to inhibit free radicals.

Investigation of antioxidant activity from beetroot juice (Beta vulgaris L) as a healthy drink for the prevention of non-communicable disease

Non-communicable diseases can cause oxidative stress, which affects their pathophysiology. One of the recommended diets for preventing complications of non-communicable diseases (NCD) is a rich antioxidant diet. One of the foods rich in antioxidants is beetroots. This study aimed to assess the antioxidant content of locally obtained beetroot juice. Laboratory experimental methods were used to test the antioxidant activity of beetroot juice. Before the laboratory test, the panelists carried out a hedonic test. The beets were then juiced and checked for DPPH′ (2,2-diphenyl-1-picrylhydrazyl). The beetroot was then juiced and checked for DPPH′ (2,2-diphenyl-1-picrylhydrazyl). Statistical analysis was performed using the Kruskal–Wallis test. Results: The hedonic test showed that panelists preferred beetroot juice with lemon juice, with a 'like' range for the aroma and viscosity and a 'neutral-like' range for the taste and color of beetroot juice. The antioxidant content in 300 ml of beetroot juice was 53,34 ± 0,13%. In conclusion, beetroot juice has a high antioxidant content and health benefits in preventing NCD.