Objective: The aim of this study to assess the sensitivity of P. aeruginosa for ATM, AML, E, CAR, NOR, CL, CN and AZM after exposure to radiation emitted from radioactive sources, Lasers and exposure to magnetized water. Study design: Cross-sectional in descriptive study design with case–control in analytical study design Backgrounds: P. aeruginosa gram negative bacteria occasion distinct kinds of contagion including epidermis, optics, ears, respiratory tract, urinary tract, intestines derived sepsis, soft tissues, skeleton and joint contagion. Radiation including ionizing and non-ionization depending on the energy of the radiated particles emitted Alpha, Beta and Gamma rays from different radioactive sources. Methodology: Study populations and bacterial deposition and identification of P. aeruginosa. Exposure P. aeruginosa to radiation at different times and doses. Antimicrobial susceptibility test for ATM, AML, E, CAR, NOR, CL, CN and AZM after exposure to radiation emitted from radioactive sources, Lasers and magnetized water. Results: After exposing P. aeruginosa to the Na23, CO60, Cs137 and Sr90 radioactive source without or without aluminum for (1,2,3) hr., the bacteria were examined for antibiotics and the results are high sensitivity to NOR, ATM, CAR. As well as, exposure to Nd: YAG laser in (10, 20) min. with exposure to magnetized water which show high sensitive to NOR and ATM antibiotics. Conclusions: The radioactive sources, Nd: YAG laser and magnetic water have a strong effect on bacteria and their sensitivity to antibiotics, so that their sensitivity rate increased very significantly compared to before exposure. The most effective antibiotics in killing bacteria after exposure are Aztreonam, Norfloxacin, Carbenicillin and Gentamycin (ATM, NOR, CAR and CN).