Kidney Tonifying Research Articles

Isolation and Identification of the Kidney Tonic Constituents of Gu-Sui-Bu (Drynaria fortunei J. Sm.)

We studied the kidney tonic components of Rhizoma Drynariae. The herbal material was extracted with 50% ethanol. Silica gel and Sephadex LH-20 column chromatography were used to isolate the chemical components using the Opossum Kidney cell as the living model. The results show that six monocases were isolated from the EtOH extract of the rhizomes of Drynaria fortunei J. Sm. Four structures were identified as naringin(1), neoeriocitrin(2), kaempferol(3), and naringenin-7-O-β-D-glucoside(4), respectively, on the basis of its spectral and physicochemical evidence. Kaempferol may promote the proliferation of the Opossum Kidney cell tested by Promega's CellTiter96 assay in vitro. Further compounds are currently being identified. Compounds 3 and 4 were isolated from this plant for the first time. Acknowledgements: Thanks go to the “Tenth Five year” Key Technologies R & D Program supported by the Ministry of Science and Technology of the People's Republic of China (2001BA701A43), and supported by Hunan Provincial Key Laboratory Plan (05FJ4049)

Effect of Kidney Tonifying Migu Tablet ("Equation missing" ) on osteoporosis rats) on osteoporosis rats

Objective: To observe the effect of Kidney tonic, the Migu Tablet ( ) on bone and endocrine of osteoporosis rats.Methods: Two stages were divided, stage 1: Using ovariectomy to establish the osteoporosis rat model; stage 2: Treating the model;Results: After 3 months treatment with MGT, which can prevent the lowering of femur bone density, tibia trabecular volume and femur bending resistant strength, but was unable to raise those lowered parameters. Serum corticosterone determination showed that the Kidney tonic could regulate the secretion of estrogen and testosterone, adjust the imbalance of E2/T ratio.Conclusions: MGT, a Kidney tonic could prevent and treat experimental osteoporosis, its imbalance of E2/T ratio adjustment is possibly one of the mechanisms to lessen the patient’s Kidney deficiency.

Study of different patterns of immuno-modulatory mechanism by tonifying Kidney and Invigorating Spleen drugs

Objective: To observe the acting pattern on the immune system by Kidney tonifying (KT) drugs.Methods: Three composite recipes were used on the 7- and 14-day corticosterone rat model to study the effect of recipes on the different patterns of immuno-modulatory and neuro-endocrinological systems.Results: The immune system of Spleen Invigorating (SI) group was well protected in either 7- or 14-day experiments, with the SI recipe showing no effect on the neuroendocrine system. It suggested that SI recipe might have direct action on immune system. On the other hand, KT group with no effect on neuroendocrine-immune (NEI) system in 7-day experiment, but showed a remarkable protective effect of the whole NEI system in 14-day experiment.Conclusion: The results indicated that KT recipe acts on the neuroendocrine system first, and then influence the immune system. It means that the action took place through down pathway of NEI network.

Anti-aging effects of Bao-Chun-Wan on rats: a morphological ultrastructure study.

Bao-Chun-Wan, a Chinese prescription, is formulated according to the principle of tonifying the kidney for treatment of aging. Morphological study on the ultrastructure of laboratory albino rats treated with this formula showed an increase in thymic lymphocytes, which play an important role in the production of T cells. As to the liver cells, the formula has a protective effect and may induce enzymes enhancing oxidation and excretion of toxic substances. An increase in the Leydig's cell, which promotes reproductive hormone secretion, was also observed, but there was no obvious change in germinal cells. The above findings reported in this article have not been cited in any previous studies and are suggesting that the kidney tonifying formula may act through the mechanism of promoting body immune function, metabolism and biotransformation, and, therefore, result in anti-aging.

Effects of Chinese Kidney-Tonifying herbal medicine on M-cholinergic receptor and acetylcholinesterase activity in dementia mimetic mice

Objective: In order to study the effects of Chinese Kidney-tonifying (KT) herbal medicine on the M-cholinergic receptor(M-R) and the acetylcholinesterase(AchE) activity in the models’ cerebral cortex.Methods: Dementia mimetic mice models were formed with aluminum chloride.Results: The Muscarine receptor total receptor concentration (M-Rt) lowered and the AchE increased in the model significantly compared with healthy young mice. KT could markedly reduce the increase of AchE and elevate the lowered M-Rt in cerebral cortex of the dementia mimetic mice and improve the memory.Conclusions: KT is effective in preventing the degeneration of cerebral function and presenile dementia.