Himalaya Research Articles

Investigation of radon and thoron exhalation rates in soils: Manali-Leh Highway region of Himalayas

This study analyses the natural radionuclides concentration (226Ra and 232Th) along with the radon and thoron exhalation rates in 50 soil samples collected from the Manali-Leh Highway region of the Himalayas. The specific activity of 226Ra and 232Th radionuclides was determined using a NaI(Tl) detector, revealing that the concentration values of these radionuclides are lower than the global average. The radon and thoron exhalation rates in the soil samples were measured utilizing a SMART RnDuo detector. The radon mass exhalation rates range from 6 to 87 mBq kg−1 h−1, with average values of 25 mBq kg−1 h−1. The thoron surface exhalation rates vary from 110 to 757 Bq m−2 h−1, with a mean value of 385 Bq m−2 h−1. Additionally, the emanation coefficient and alpha dose equivalent for radon were calculated and the correlation between parent and daughter radionuclides was also explored.

Comparing traditional and automated conservation assessments for Himalayan species of Buddleja

Abstract To compare the benefits and drawbacks of traditional and automated conservation assessments, we used a field-based study and automated conservation assessments using GeoCAT, red and ConR to assess four species of Buddleja (Scrophulariaceae), a cosmopolitan genus of flowering plants. Buddleja colvilei, Buddleja sessilifolia, Buddleja delavayi and Buddleja yunnanensis are endemic to the Himalayan region. They have not yet been assessed for the IUCN Red List of Threatened Species but are facing elevated risks of extinction because of various anthropogenic and environmental pressures. Buddleja sessilifolia and B. delavayi are listed as Plant Species with Extremely Small Populations in Yunnan, China, where they are known to be threatened. Although automated assessments evaluated B. delavayi and B. yunnanensis as Endangered and B. sessilifolia and B. colvilei as Vulnerable, our field studies indicated a different categorization for three of the species: B. delavayi and B. yunnanensis as Critically Endangered and B. sessilifolia as Endangered. Our findings indicate that the accuracy and reliability of assessment methods can differ and that field surveys remain important for conservation assessments. We recommend an integrated approach addressing these limitations, to safeguard the future of other species endemic to the Himalayan region.

Understory growth of Paris polyphylla accumulates a reservoir of secondary metabolites of plants.

Paris polyphylla is an important traditional medicinal plant of the Himalayan region. It is extensively used for the production of natural steroidal saponins and flavonoids. Although seed dormancy of wild plants can be broken to be artificially maintained and regenerated through micropropagation in the laboratory, the success of secondary metabolite production in higher quantities and the synthesis of superior plant metabolites have been very limited. In this study, we present differential metabolic profiling of P. polyphylla plants maintained for 8 years in natural and greenhouse conditions. Untargeted profiling of the metabolites through ultra-performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS), followed by statistical analysis, identified secondary metabolites that were enriched in the naturally occurring plant roots compared with the greenhouse plant roots. A multivariate statistical analysis revealed the differential distribution of the compounds between the two groups. Overall, we identified 1,182 secondary metabolites, with 116 metabolites being differentially upregulated and 256 metabolites being downregulated. Moreover, 810 metabolites showed no significant variation under both growing conditions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the naturally forest-grown P. polyphylla plants were significantly enriched in steroidal saponins, lipids, vitamins, flavonoids, and flavonols. An analysis of the top 10 differentially upregulated secondary metabolites indicated a significantly enriched quantity of spirost-5-en-3,12-diol and kaempferol synthesis pathways, which are known to reduce the effect of free radicals scavengers inside the cell. In addition, veratramine alkaloids were also enriched under natural conditions. Our findings indicated that naturally maintained P. polyphylla plants are suitable for the extraction of medicinally important compounds. Our study established a causal relationship between the metabolic composition of the roots and their natural growth condition. This study highlighted the importance of environmental conditions in the biosynthesis of secondary metabolites of plants.

Spatial heterogeneity in unintended pregnancy and its determinants in India

BackgroundUnderstanding the geographic variation of unintended pregnancy is crucial for informing tailored policies and programs to improve maternal and child health outcomes. Although spatial analyses of unintended pregnancy have been conducted in several developing countries, such research is lacking in India. This study addresses this gap by investigating the geographic distribution and determinants of unintended pregnancy in India.MethodsWe analysed data from the National Family Health Survey-5 encompassing 232,920 pregnancies occurring between 2014 and 2021 in India. We conducted a spatial analysis to investigate the distribution of unintended pregnancies at both state and district levels using choropleth maps. To assess spatial autocorrelation, Global Moran’s I statistic was employed. Cluster and outlier analysis techniques were then utilized to identify significant clusters of unintended pregnancies across India. Furthermore, we employed Spatial Lag Model (SLM) and Spatial Error Model (SEM) to investigate the factors influencing the occurrence of unintended pregnancies within districts.ResultsThe national rate of unintended pregnancy in India is approximately 9.1%, but this rate varies significantly between different states and districts of India. The rate exceeded 10% in the states situated in the northern plain such as Haryana, Delhi, Uttar Pradesh, Bihar, and West Bengal, as well as in the Himalayan states of Himachal Pradesh, Uttarakhand, Sikkim, and Arunachal Pradesh. Moreover, within these states, numerous districts reported rates exceeding 15%. The results of Global Moran’s I indicated a statistically significant geographical clustering of unintended pregnancy rates at the district level, with a coefficient of 0.47 (p < 0.01). Cluster and outlier analysis further identified three major high-high clusters, predominantly located in the districts of Arunachal Pradesh, northern West Bengal, Bihar, western Uttar Pradesh, Haryana, Delhi, alongside a few smaller clusters in Odisha, Madhya Pradesh, Uttarakhand, and Himachal Pradesh. This geographic clustering of unintended pregnancy may be attributed to factors such as unmet needs for family planning, preferences for smaller family sizes, or the desire for male children. Results from the SEM underscored that parity and use of modern contraceptive were statistically significant predictors of unintended pregnancy at the district level.ConclusionOur analysis of comprehensive, nationally representative data from NFHS-5 in India reveals significant geographical disparities in unintended pregnancies, evident at both state and district levels. These findings underscore the critical importance of targeted policy interventions, particularly in geographical hotspots, to effectively reduce unintended pregnancy rates and can contribute significantly to improving reproductive health outcomes across the country.

Analyzing the Spatiotemporal Changes in Climatic Extremes in Cold and Mountainous Environment: Insights from the Himalayan Mountains of Pakistan

This study assessed the past changes in extreme precipitation and temperature events across the Himalayan Mountains of Pakistan. This cold and mountainous environmental region has witnessed a significant increase in climate-related disasters over the past few decades. Spatiotemporal changes in extreme temperature and precipitation events were analyzed using 24 indices developed by the Expert Team on Climate Change Detection and Indices (ETCCDI). For this study, in situ data of 16 national meteorological stations were obtained from the Pakistan Meteorological Department (PMD) for the past three decades (1991–2020). The significance of the trends was assessed using the modified Mann–Kendall (MMK) test, and the Theil–Sen (TS) slope estimator was used to estimate the slope of the trend. The results showed that there has been a consistent decline in the total precipitation amount across the Himalayan Mountains of Pakistan. The trend exhibited a decrease in the annual average precipitation at a rate of −6.56 mm/year. Simultaneously, there was an increasing trend in the annual average minimum and maximum temperatures at rates of 0.02 °C/year and 0.07 °C/year, respectively. The frequencies of consecutive wet days (CWDs) and maximum 5-day precipitation (RX5day) have decreased significantly, with decreasing rates of −0.40 days/year and −1.18 mm/year, respectively. The amount of precipitation during very wet days (R95p) and extremely wet days was decreased by −19.20 and −13.60 mm/decade, respectively. The warm spell duration (WSDI) and the frequency of warm days (TX90p) across the Himalayan Range both increased by 1.5 and 1.4 days/decade. The number of cold days (TX10p) and cold nights (TN10p) decreased by 2.9 and 3.4 days/decade. The average temperature of the hottest nights (TXn) and the diurnal temperature range (DTR) were increased by 0.10 and 0.30 °C/decade. The results indicated an increasing tendency of dry and warm weather in the Himalayan region of Pakistan, which could have adverse consequences for water resources, agriculture, and disaster management in the country. Therefore, it is essential to prioritize the implementation of localized adaptation techniques in order to enhance sustainable climate resilience and effectively address the emerging climate challenges faced by these mountainous regions.

Shifting distribution patterns of an endemic conifer species in the Himalayan region under climate change: Past, present, and future

Species with narrow ranges, particularly rare and endangered ones, are more vulnerable to rapid climate change. The endangered Pinaceae species Picea smithiana (Wall.) Boiss. (P. smithiana), endemic to the Himalayan region, is of significant economic and ecological importance. Understanding how its distribution responds to climate change is crucial for effective biodiversity conservation. Our research integrates three species distribution models, i.e. MaxEnt, Random Forest, and Support Vector Machine, to predict the suitable habitat of P. smithiana in the past, present, and future, and to analyze changes in their spatial distribution patterns. Our study also interprets its geographical distribution patterns through an analysis of multiple environmental factors within its suitable habitat. Our results indicate that, since the last glacial maximum, the suitable habitat of P. smithiana has been shifting towards high latitudes and altitudes, corresponding with its cold tolerance traits. Besides the Himalayan region, suitable habitat of P. smithiana were also predicted in Southwest China, despite a lack of observed occurrences, suggesting possible speciation events linked to the uplift of the Qinghai−Tibetan Plateau. Our study warns that the actual distribution of P. smithiana might be more pessimistic than predictions indicate, with protected areas encompassing less than 27 % of its suitable habitat. This highlights the critical need for intensified conservation and management measures to secure the long-term persistence of P. smithiana.

Impact of regional climate change on apple yield in Himachal Pradesh, India

Climate change has become a major global as well as regional challenge because of its adverse effects on biodiversity and agriculture. Himachal Pradesh, a state in India, is situated in the western Indian Himalayan region, which predominantly features an agricultural economy, with apple being the major cash crop of the region. This paper presents the trend analysis of climatic parameters to delineate the effect of climate change on the apple yield in the region from 1991 to 2018. The temperature and rainfall data were obtained from the Indian metrological department, while snowfall data and ambient air quality data were collected from the database of NASA. The chill unit was calculated using the UTAH model. The climatic parameters were compared with apple yield to identify the impact of climate change on the crop. Therefore, apple yield reduction risk was calculated. The trend analysis indicated a temperature decrease during the monsoon months and a temperature increase during winters. The same analysis also indicated decreased snowfall by 0.375 kg/m2 and increased rainfall during the spring season by 23.2 mm, 19.6 mm, 10.4 mm (between mid-February and mid-April from 1985 to 2020). The chill units and apple production showed a positive correlation. In summary, our results indicated that climate change has reduced the apple yield by 11.48%.

Impact of bulky manures and fermented liquid formulations in enhancing the quality parameters of sweet pepper

Indigenous fermented liquid formulations (Panchagavya, Jeevamrut and Amritpani) are rich sources of beneficial microflora and fauna, vitamins, minerals and growth-promoting hormones. Small and marginal farmers of North-West Himalayan region popularly grow sweet pepper as commercial crop. Poultry manure and vermicompost are two cheap nutrient sources, popular among these growers. So, inorder to investigate the combined effect of these organic inputs, two-year field experiment with seven treatments and three replications; with randomized block design; comprised of 100, 90, 80, 70, 60, 50, 40 per cent recommended dose of nutrients (RDN) which was applied through vermicompost and poultry manure along with Panchagavya (5%), Jeevamrut (5%), Amritpani (5%) and plant growth promoting rhizobacteria which was isolated from experimental location, applied to all treatments, except T1; was carried out to study the response of these inputs on quality parameters of sweet pepper. Results revealed that treatment T2 that comprised of 90% RDN including Panchagavya, Jeevamrut, Amritpani and PGPR led to noticeably improvement in growth and quality parameters viz. chlorophyll-a (0.52 mg g−1), chlorophyll-b (0.73 mg g−1), ascorbic acid (29.47 mg 100 g−1), protein content (28.90%), capsaicin content (0.23 mg g−1), number of primary branches (8.20), average fruit weight (62 g), fruit yield (290.58 quintals ha−1), number of flowers per plant (24.97), leaf area index (4.73) and shelf life (6.03 days). Hence, it is concluded that the combined use of these inputs has the potential to enhance growth and quality characteristics of sweet pepper in achieving sustainable crop productivity.

Black-necked crane as border object: interspecies politics in Himalayan borderlands

ABSTRACT Legacies of imperial frontier-making, ongoing border conflicts between nuclear armed nations, changing livelihoods, and the impact of anthropogenic climate change have created complicated claims on Himalayan borderland areas in the name of conservation, security, ethnic identity and development. Combining historical research with ethnography, this article traces the role of the Black-necked crane (grus nigricollis) in the formation of mid-nineteenth century imperial frontiers in the Himalayas and, subsequently, its present-day position in the political ecology of two Himalayan regions along the Sino-India border – Ladakh and Arunachal Pradesh. We introduce the concept of ‘border object’ and demonstrate how it can serve as a useful concept to understand how non-humans become participants in changing configurations of territoriality, biopolitics and science, which underpin both a past imperial frontier and present-day border regimes.

Notes on Günther’s Oriental Slender Snake (&lt;i&gt;Trachischium guentheri&lt;/i&gt;) from the Himalayan Realm of North Sikkim, India

The Indian Himalayan Region (IHR) is known for its biodiversity, yet it faces substantial threats fromanthropogenic activities and climate change. Despite the ecological importance of the IHR, herpetofaunal studies,especially in the eastern Himalayan State of Sikkim, remain limited. We herein address aspects of natural history ofa fossorial snake, Günther’s Oriental Slender Snake (Trachischium guentheri) in North Sikkim. We encountered 25snakes during field surveys from 30 September to 12 October 2023 in the Lachen region at elevations of 2,549–2,918m asl. Responses of snakes to unusually high rainfall led to increased road mortality during the peak tourist season. Inresponse, we propose two specialized structures, featuring elevated barriers and elaborate hollow, trapezoidal concreteslabs, to mitigate roadkills. We also emphasize the importance of monitoring to identify areas with high traffic wheremeasures to mitigate road mortality should be employed.

Integrative taxonomic review of the genus Rhodostrophia Hübner, 1823 and its allied genus Tanaotrichia Warren, 1893 (Lepidoptera: Geometridae) from the Western Himalaya

The genus Rhodostrophia Hübner, 1823 and its close ally Tanaotrichia Warren, 1893 (Geometridae: Sterrhinae) are reviewed from the North Western and Trans Himalayan regions of the Indian Himalaya along with some specimens from the Pakistan. In total, eight species were studied—seven Rhodostrophia spp. and one species of Tanaotrichia—with detailed morphological and genital characteristics. Rhodostrophia borealis stat. n. is raised from subspecies of R. cinerascens to species level after studying external morphology and distinct genitalia-based diagnostic features. However, this warrants further validation following the barcode sequencing of R. cinerascens. Illustrations of habitus, including type specimens, are provided along with images of genitalia and the sequence data of the mitochondrial cytochrome c oxidase subunit I (COI 5′ terminus) gene. A checklist of Indian Rhodostrophia and Tanaotrichia species is included with information on their documented distributional range.

Influence of bark chemistry on distribution of epiphytic mosses on basal trunk of &lt;i&gt;Cryptomeria japonica&lt;/i&gt;

Epiphytic mosses are integral parts of forest community structure in the Darjeeling Hills of the Eastern Himalayan region with remarkable contributions to the ecosystem functionality. The study was framed to assess the richness and spatial distribution of epiphytic mosses growing on the basal trunk of Cryptomeria japonica (Thunb. ex. L.) D.Don, and also to evaluate the explanatory host traits for shaping the moss assemblage. Field measurements and sampling were performed near Lamahatta village within Darjeeling district on 270 microplots placed on tree trunks. A total of twelve mosses represented by the members of Dicranales and Hypnales were recorded. Low species diversity was observed with dominance and maximum cover of Syrrhopodon confertus. Canonical correspondence analysis predicted a distinct combination of chemical requirements for local colonization of each moss. The results also demonstrated influence of bark acidic inputs on abundance and co-existence of bryophytes. The outcome can be potentially helpful in depicting the community structure of non-vascular epiphytes, which may further be considered while developing forest management strategies.

Geochemistry of Mafic Rocks From the Nagrota–Kathindi Section, Himachal Pradesh, Northwestern Himalaya: A Probable Example of Plume–Lithosphere Interaction

ABSTRACTThe Northwest Himalayan region has a record of several phases of mafic magmatic activity spanning from Precambrian to Cenozoic in a dynamic tectonic setting. Here, we studied detailed petrography and new whole‐rock geochemistry of mafic volcanic and dykes from the Nagrota–Kathindi Section (NKS), Himachal region of the NW Himalaya, to understand the petrogenesis and possible tectonic setting. Both rock types have comparable mineralogical compositions (clinopyroxene + plagioclase + actinolite‐tremolite + chlorite + iron oxides ± hornblende ± epidote ± quartz ± carbonates) overprinted by greenschist to lower amphibolite facies metamorphism. The mafic volcanic and dykes of NKS exhibit subalkaline basalts to basaltic andesites and a typical tholeiite compositional character. The chondrite‐normalized rare earth element pattern exhibits similar LREE‐enrichment and strong HREE‐fractionation, whereas primitive mantle‐normalized multi‐element patterns show pronounced LILE‐enrichment of Rb, Ba, Th, LREE, and HFSE depletion of Nb, K, P, and Ti. The Zr–Y–Nb–Th relationships indicate that both rock types were derived from the plume source, whereas low Nb/La (&lt; 1), similar high large‐ion lithophile element concentrations, and pronounced negative Nb, Zr, P, and Ti anomalies suggest that components other than mantle plume must have been involved in the generation and evolution of both rock types, that is, most likely plume and subcontinental lithosphere mantle (SCLM) interaction. The genesis of parent magma for the NKS volcanic and dykes was derived by 4%–6% and 10%–20% partial melting from the spinel + garnet lherzolite stability field. The majority of the studied samples correspond to spinel + garnet peridotite melting on (Gd/Yb)N versus CaO/Al2O3 diagram, thereby corroborating residual garnet in the mantle restite. All the basalts and dykes from the NK section did erupt/intrude in an intracontinental rift setting based on geochemical discrimination. The key petro‐tectonic processes attributed to the formation of these rocks are as follows: (i) the melting of the ascending plume by adiabatic decompression; (ii) the partial melting of this plume–SCLM source in the melting regime, which produces basaltic magma with a tholeiitic composition; and (iii) the release of heat that provides the thermal condition for melting of SCLM and interaction between upwelling mantle plume and subduction metasomatized SCLM.

Geospatial Analysis of Soil Erosion and Subsequent Geomorphic Hazards in the Eastern Himalayan Region, India

Geospatial Analysis of Soil Erosion and Subsequent Geomorphic Hazards in the Eastern Himalayan Region, India

Experimental and Reliability Study of Nano-Silica Stabilized Problematic Soil in Lesser Himalayan Region

Experimental and Reliability Study of Nano-Silica Stabilized Problematic Soil in Lesser Himalayan Region

Inter–Intraseasonality of Meteorological Drivers of Chorabari Glacier, Central Himalaya: Implications for Mass Fluctuations and Associated Hazards

Abstract High-altitude meteorological data are scarce in the Himalayan region, particularly for glacierized catchments. In-depth knowledge of meteorological variables is crucial to describe numerous cryospheric processes and associated hazards. The present study comprehensively assesses time series data (2013–16) of meteorological parameters in the Chorabari Glacier catchment (CGC) using an automatic weather station (∼4270 m MSL). Results revealed that average near-surface air temperature varied seasonally, ranging from 6.4°C in the summer monsoon (June–September) to −6.3°C in the winter season (December–February). The minimum temperature (Tmin) remained positive throughout the monsoon seasons in the higher elevations (∼5050 m MSL), indicating the melting of glaciers and their reduced mass. The daily relative humidity in different seasons fluctuated from 39% to 90%, whereas the highest seasonal average (∼63%) was observed in the monsoon season. The average wind speed was high (∼1.7 m s−1) during winter and dropped to 1.1 m s−1 in the monsoon season. The average daily influx of solar radiation ranged from 19 to 374 W m−2, with the highest value recorded during the premonsoon (March–May ∼ 212 W m−2) and the lowest during the winter (∼149 W m−2) season. The daily outflux solar radiation varied from 18 to 186 W m−2, with the maximum value in premonsoon (∼145 W m−2) and the minimum value in the monsoon season (∼30 W m−2). The mean zero-degree isotherm was found above the equilibrium line altitude (ELA ∼ 5120 m MSL) during the monsoon season, indicating a considerable mass depletion of the Chorabari Glacier. The abnormal variations in climatic variables on 16–17 June 2013 were signs of the Kedarnath disaster. Significance Statement The main objective of this study is to understand the variability of meteorological drivers at daily, monthly, and seasonal scales and how these changes may impact glacier mass and downstream hydrology. Our research revealed considerable variations in maximum temperature throughout the year and positive minimum temperature at higher elevations during monsoon months, which significantly control the glacier mass changes. Meteorological variables showing cyclic behavior and indicators of the “monsoon lowering phenomenon” are important in determining the timing and intensity of the seasonal shift. Investigation of abnormal changes in the climatic drivers in the 2013 Kedarnath disaster provides valuable insight into the glacier-related hazards and draws attention to decision-makers to create robust strategies to monitor the dynamic response of Himalayan glaciers.

Influence of the Western Disturbance on Winter Precipitation: Observations over the Central Himalayas using VHF Radar

This study investigates the influence of the Western Disturbance (WD) on atmospheric wind patterns and associated precipitation over the Himalayan region utilizing observations carried out using the ARIES ST Radar (ASTRAD) at Nainital [29.36° N, 79.46° E, 1800 m] during March 2024. The observations reveal that during the WD events, the wind direction was predominantly south westerly/westerly with varying speeds, reaching up to 67 m/s. The HYSPLIT model back-trajectory analysis confirmed that the moisture-laden air masses originated from the Atlantic Ocean. Vertical wind measurements indicated significant atmospheric instability and convection, characterized by updraft and downdraft motions in three distinct height regions between 8 km and 10 km, an updraft between 6 km and 7 km, and another downdraft between 4.5 km and 5 km. The core height region of the subtropical westerly jet (SWJ) embedded in WD and shift in height with maximum wind speed are also identified.

Unveiling the microbiome and metabolites of traditional dairy and alcoholic products from North-western Himalayan region

The people of the Indian North-western Himalayas consume various traditional fermented foods, which may contribute to their healthy lifestyle. However, the probiotic diversity of the region is largely unknown. In the current study, indigenous Himalayan traditional foods (dairy products and alcoholic beverages) were investigated for probiotic diversity via culture-based and culture-independent methods and metabolites study. The culture-based taxonomic classification revealed the prevalence of probiotic genera Levilactobacillus, Latilactobacillus, Limosilactobacillus, Lactiplantibacillus, Lacticaseibacillus, and Companilacobacillus, which differed from the genera identified through amplicon sequencing. Alpha diversity analysis revealed higher probiotic diversity in fermented dairy products (FDPs) than in the alcoholic beverages samples. Redundancy analysis revealed that food’s properties, like fat, ash, & moisture content, temperature, and electrical conductivity, influence the probiotic diversity in the samples. Additionally, Gas chromatography-mass spectrometry (GC-MS) analysis identified high levels of methyl hexadecanoate and methyl linoleate in the food products. Metabolic functional predictions showed the dominance of anabolic pathways in dairy samples and catabolic pathways in alcoholic beverage samples. Overall, FDPs with superior nutritional properties and probiotic diversity could be considered a better choice as functional food.

First Comprehensive Evaluation of Genetic Variability for Bacterial Blight Resistance in Wild Punica granatum L. Populations from the North-Western Himalayas

Identifying and selecting disease-resistant sources from wild germplasm pools is pivotal due to their ecological benefits and cost-effectiveness. This study encompassed the survey and collection of wild pomegranate genotypes alongside commercial cultivars, followed by screening against native pathogens. Additionally, biochemical analyses were conducted to elucidate defense mechanisms in both resistant and susceptible genotypes. Cuttings from selected germplasm across four districts were obtained, grown for one year, and assessed for pathogenicity and biochemical responses. Microscopic and molecular analyses confirmed the pathogen as Xanthomonas axonopodis pv. punicae (Xap), identified by its distinctive morphology and a 1500 bp amplicon along with phylogeny analysis. Germplasm screening via detached and attached leaf assays identified genotypes with minimal disease severity, highlighting SH-14 and SH-16 as highly resistant in the attached leaf assay. Principal component analysis categorized genotypes based on disease parameters, with PC1 explaining approximately 75% of the variability. Agglomerative hierarchical clustering further grouped genotypes into ten clusters, emphasizing clusters IX and X for their significant resistance traits. Biochemical studies revealed that the resistant genotype SH-16 exhibited higher levels of constitutive defense components like chlorophyll and vitamin C, as well as induced defense components such as phenols, flavonoids, and antioxidants, compared to the susceptible cultivar Bhagwa. These findings underscore the critical roles of both constitutive and induced defense mechanisms in conferring pomegranate resistance to Xap. The study's comprehensive approach in evaluating genetic and biochemical resistance provides valuable insights for breeding disease-resistant pomegranate varieties, marking a significant advancement in exploring pomegranate wild relatives from the Himalayan region for their resistance to bacterial blight.

Effectiveness of NGOs in mountainous solid waste management: A case study from Healing Himalayas in Rakchham, Himachal Pradesh, India.

Non-governmental organizations (NGOs) play a critical role in addressing solid waste management (SWM) challenges in remote mountain communities, including the ecologically fragile Himalayan region. This study evaluates the impact of Healing Himalayas, an NGO, in Rakchham village, Himachal Pradesh, India. The objectives were to evaluate the effectiveness of Healing Himalayas' decentralized SWM model in promoting stakeholder engagement and resource recovery, assess the role of collaborations between local authorities and the NGO in financing waste management practices, investigate the influence of tourism and seasonal variations on solid waste generation patterns and waste management practices in Rakchham, and material recovery facilities, followed by glass (36.7%), paper/cardboard (18.4%) and metal (4.1%). A fee-based system involving the local village council funded waste operations. Waste generation exhibited significant seasonal fluctuations, with tourism influxes driving increased volumes. Healing Himalayas' initiatives promoted community participation, with over 15 awareness workshops conducted. Key challenges included limited financial resources, inadequate infrastructure, lack of advanced treatment facilities and need for context-specific solutions like efficient wet waste management in cold climates. The study highlights Healing Himalayas' decentralized model's success in fostering stakeholder engagement, behavioural change and resource recovery. The findings inform effective strategies for NGO-led waste management initiatives tailored to remote Himalayan communities.