Key Control Point Research Articles

Establishment and application of soil hydraulic erosion model based on GIS and USLE model

Soil erosion is the most common form of soil degradation, which is the main problem of soil environmental destruction worldwide. Based on the USLE and GIS remote sensing inversion and superposition analysis technology, this study simulated and calculated soil hydraulic erosion in Fangshan World Geological Park, China in 2011, 2012 and 2013, analyzed its spatial-temporal dynamic changes, and explored the key points of soil erosion control in the study area. The results show that (1) The main erosion occurred at 25°-35° steep slope and 400-800m height, which accounted for more than 30% of the total soil erosion in all the three years. (2) The average erosion modulus and erosion amount in 2012 were significant higher than that in 2011 and 2013 at each slope grade, shown that the soil erosion of 2012 is influenced by natural disaster.With the increase of slope grade, the average modulus also increased.

Analysis on the Maintainability of Smart Meter Software for Full Lifetime

With the deepening of Internet concept and electricity marketization reform, more requirements are put forward for metering equipment, and the scale and complexity of software also increase. The new generation of intelligent electricity meters meet the IR46 standard and adopt the multi-core modular design scheme, which also puts forward higher requirements for the maintainability of software. This paper starts from the key control points of the whole life cycle of intelligent electricity meters and combines the characteristics of software testing, so as to analyze and evaluate the maintainability of the software. Firstly, the factors that influence the maintainability of watt-hour meter products are analyzed, and the software key data is considered from the perspective of software development and design. Then, the index system of the maintainability of the intelligent software is established, and the key points of the maintainability test and the weight of each index are analyzed in combination with the testing technology of the intelligent meter software and the analytic hierarchy process.

1918 год: «нулевая» германская оккупация российского Дона

The internal situation in the Don region in 1918, during the intervention of armed units of the Austrian and German armies, has been overshadowed in the scholarship by two key phenomena: fates of the Volunteer Movement and formation of the quasi-state, All-Great Don Host. It is important to reconstruct the events that took place in the Don towns and villages in May–November 1918. Historical sources are scattered throughout archives and libraries. The author has used fonds of the State Archive of the Russian Federation, the State Archive of the Rostov Region, and the Center for Documentation on the Contemporary History of the Rostov Region. Austrian and German units that appeared on the borders of the Don region in early May 1918 engaged in combat only with the Red Army detachments. Cossacks and foreign troops fought together from the very beginning. In future, the German administration strove to organize uninterrupted supplies of industrial raw materials and products, food and fodder from the Don territory. In order to do this, the Germans occupied key control points and transport communications in the Western part of the region. A double government was introduced in the villages: alongside atamans there appeared German commandants. Re-election of Ataman P. N. Krasnov in August 1918 was ensured by the Germans; his most influential opponents were neutralized; censorship for the press was introduced. The Germans held a neutral position towards Russian officers and the Volunteer Army. The experience of intervention in the South of Russia influenced the fate of Germany, as German soldiers received a practical lesson in revolutionary action. Presence of the Central Powers’ troops in Russia forced the Entente countries to intervene more actively in the affairs of their former ally. Germany assumed that successful results of the armistice on the Eastern Front could be replicated on the Western Front.

Occupational Dust Hazards and Risk Assessment of Coal-Fired Thermal Power Plants of Different Capacities - China, 2017-2019.

What is already known about this topic?Silica dust and coal dust are the main occupational hazards in coal-fired thermal power plants, which mainly exist in coal transportation workplaces, combustion milling workplaces, and ash removal workplaces.What is added by this report?The overall environmental and personal dust exposure levels decrease with an increase in the capacity of coal-fired thermal power plants, the overall dust hazard risk level of the workforce in coal-fired is Medium.What are the implications for public health practice?Dust management should be conducted in the coal-fired thermal power plant in 300 million watt units because it has the highest dust exposure level, and ash removal workplaces and combustion milling workplaces are key control points for dust hazards.

Lactate Limits T Cell Proliferation via the NAD(H) Redox State

SUMMARYImmune cell function is influenced by metabolic conditions. Low-glucose, high-lactate environments, such as the placenta, gastrointestinal tract, and the tumor microenvironment, are immunosuppressive, especially for glycolysis-dependent effector T cells. We report that nicotinamide adenine dinucleotide (NAD+), which is reduced to NADH by lactate dehydrogenase in lactate-rich conditions, is a key point of metabolic control in T cells. Reduced NADH is not available for NAD+-dependent enzymatic reactions involving glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate dehydrogenase (PGDH). We show that increased lactate leads to a block at GAPDH and PGDH, leading to the depletion of post-GAPDH glycolytic intermediates, as well as the 3-phosphoglycerate derivative serine that is known to be important for T cell proliferation. Supplementing serine rescues the ability of T cells to proliferate in the presence of lactate-induced reductive stress. Directly targeting the redox state may be a useful approach for developing novel immunotherapies in cancer and therapeutic immunosuppression.

Application of Big Data Analysis in Flood Control of Small Reservoirs -A Case study of Liaoning Province, China

Small reservoirs play an important role in the safety of flood control and economic development. Their operation and management are characterized by a large number of objects and a large amount of information, which requires effective data analysis means support. Based on this, big data analysis technology has been gradually applied and popularized in the operation and management of small reservoirs. In this paper, we first set up a small reservoir flood reporting system based on the data collection and analysis of various aspects such as reservoir capacity, characteristic water level, real-time data of water and rain situation, meteorological data, and presented the results in the paper. Secondly, we analyzed the under-reporting situation during the flood control period of the reservoir, and explored the under-reporting factors and the time when the under-reporting occurred. Finally, we conduct a time series analysis on the situation of water level exceeding the limit of 50 reservoirs during the flood control period, so that we can find the key reservoirs and important time points of flood control more intuitively and provide reference for the smooth development of the management of small reservoirs.

A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia

Intratumoral heterogeneity is a common feature of many myeloid leukemias and a significant reason for treatment failure and relapse. Thus, identifying the cells responsible for residual disease and leukemia re-growth is critical to better understanding how they are regulated. Here, we show that a knock-in reporter mouse for the stem cell gene Musashi 2 (Msi2) allows identification of leukemia stem cells in aggressive myeloid malignancies, and provides a strategy for defining their core dependencies. Specifically, we carry out a high throughput screen using Msi2-reporter blast crisis chronic myeloid leukemia (bcCML) and identify several adhesion molecules that are preferentially expressed in therapy resistant bcCML cells and play a key role in bcCML. In particular, we focus on syndecan-1, whose deletion triggers defects in bcCML growth and propagation and markedly improves survival of transplanted mice. Further, live imaging reveals that the spatiotemporal dynamics of leukemia cells are critically dependent on syndecan signaling, as loss of this signal impairs their localization, migration and dissemination to distant sites. Finally, at a molecular level, syndecan loss directly impairs integrin β7 function, suggesting that syndecan exerts its influence, at least in part, by coordinating integrin activity in bcCML. These data present a platform for delineating the biological underpinnings of leukemia stem cell function, and highlight the Sdc1-Itgβ7 signaling axis as a key regulatory control point for bcCML growth and dissemination.

Key Points of Quality Control of Landscape Greening Construction

Key Points of Quality Control of Landscape Greening Construction

Laboratory Management and Quality Control Practice of SARS-CoV-2 Nucleic Acid Detection.

ObjectiveA positive result of SARS-CoV-2 nucleic acid detection provides critical laboratory evidence for clinical confirmed diagnosis, pandemic status evaluation, a pandemic prevention plan, treatment of infected people with symptoms, and protection of uninfected people. This study aims to provide a practical reference for SARS-CoV-2 nucleic acid–related research and detection.MethodsOur laboratory has established policies combining personnel management and quality control practices for SARS-CoV-2 nucleic acid detection during the pandemic.ResultsIn this article, we describe cross-department personnel management and key points of personal protection and quality control in the testing process. We also report on the differences in detection and the compatibility between different brand kits.ConclusionIt is critical to maintain a standard and accurate laboratory operation for nucleic acid testing.

Dynamic Control Method and Application of Coastal Engineering Project Construction Cost Based on Rough Sets

Zhang, D. and Liu, X., 2020. Dynamic control method and application of coastal engineering project construction cost based on rough sets. In: Bai, X. and Zhou, H. (eds.), Advances in Water Resources, Environmental Protection, and Sustainable Development. Journal of Coastal Research, Special Issue No. 115, pp. 7-9. Coconut Creek (Florida), ISSN 0749-0208.The traditional cost analysis method of construction engineering has low operation efficiency and poor convergence performance, which leads to inaccurate cost analysis. With the increasingly fierce competition in the construction market, construction enterprises must strengthen the control and management of project costs in terms of systems and measures in order to continuously find profit space in low-cost and low-profit projects. Due to the complexity of the construction process and the uncertainty of many influencing factors, it is difficult to formulate a scientific cost control strategy, predict the degree of influence of various cost factors and reveal the correlation between them and the total cost before construction. Construction cost management of engineering project is the most concerned topic in coastal engineering project, and also the basis and key point of cost control of coastal engineering project. In order to judge the importance of cost factors and find the relationship between cost factors in coastal engineering projects so as to realize scientific cost control decision-making, it is necessary to objectively judge the main influencing factors of cost based on as many cost data as possible.

QUALITY ANALYSIS AND IMPROVEMENT OF FUNDAMENTAL GEOGRAPHIC NATIONAL CONDITIONS MONITORING RESULTS

Abstract. With the integration and unification of natural resources, the application fields and application levels of geographic national condition monitoring results have also expanded and improved, and the data quality requirements for national condition monitoring have become higher and higher, especially the results of fundamental geographic national conditions monitoring, the quality of the results is the basis of monitoring and the life of monitoring. Based on the recent practice in fundamental geographic national conditions monitoring, this paper summarizes the quality requirements of monitoring results, analyzes typical quality problems and quality influencing factors, and three measures for improving the quality of the results are proposed: one is a problem-oriented quality inspection method; the other is the key points of quality control in the fundamental national condition monitoring process; the third is the preliminary exploration of the application of big data in monitoring.

Cytosolic Invertase-Mediated Root Growth Is Feedback Regulated by a Glucose-Dependent Signaling Loop.

The disaccharide Suc cannot be utilized directly; rather, it is irreversibly hydrolyzed by invertase to the hexoses Glc and Fru to shape plant growth. In this context, Glc controls the stability of the transcription factor Ethylene-Insensitive3 (EIN3) via the function of Hexokinase1 (HXK1), a Glc sensor. Thus, invertase, especially the major neutral cytosolic invertase (CINV), constitutes a key point of control for plant growth. However, the cognate regulatory mechanisms that modulate CINV activity remain unclear. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), EIN3 binds directly to both the promoters of Production of Anthocyanin Pigment1 (PAP1) and Phosphatidylinositol Monophosphate 5-Kinase 9 (PIP5K9), repressing and enhancing, respectively, their expression. Subsequently, PAP1 binds directly to and promotes transcription of the Cytosolic Invertase1 (CINV1) promoter, while PIP5K9 interacts with and negatively regulates CINV1. The accumulated CINV1 subsequently hydrolyzes Suc, releasing the sequestered signaling cue, Glc, which has been shown to negatively regulate the stability of EIN3 via HXK1. We conclude that a CINV1-Glc-HXK1-EIN3-PAP1/PIP5K9-CINV1 loop contributes to the modulation of CINV1 activity regulating root growth by Glc signaling.

Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.

The phytohormone abscisic acid (ABA) is the best‐known stress signaling molecule in plants. ABA protects sessile land plants from biotic and abiotic stresses. The conserved pyrabactin resistance/pyrabactin resistance‐like/regulatory component of ABA receptors (PYR/PYL/RCAR) perceives ABA and triggers a cascade of signaling events. A thorough knowledge of the sequential steps of ABA signaling will be necessary for the development of chemicals that control plant stress responses. The core components of the ABA signaling pathway have been identified with adequate characterization. The information available concerning ABA biosynthesis, transport, perception, and metabolism has enabled detailed functional studies on how the protective ability of ABA in plants might be modified to increase plant resistance to stress. Some of the significant contributions to chemical manipulation include ABA biosynthesis inhibitors, and ABA receptor agonists and antagonists. Chemical manipulation of key control points in ABA signaling is important for abiotic and biotic stress management in agriculture. However, a comprehensive review of the current knowledge of chemical manipulation of ABA signaling is lacking. Here, a thorough analysis of recent reports on small‐molecule modulation of ABA signaling is provided. The challenges and prospects in the chemical manipulation of ABA signaling for the development of ABA‐based agrochemicals are also discussed.

Research on service quality evaluation method of cold chain logistics of agricultural products based on key control points

Based on the idea of key control points, the evaluation model of cold chain logistics service quality of fresh agricultural products is constructed. Based on the analysis of the characteristics of cold chain logistics of fresh agricultural products, combined with SERVQUAL model and LSQ model, four key control points of service quality of cold chain logistics of fresh agricultural products are selected, and the fuzzy comprehensive evaluation method is used to analyze the evaluation examples. The results show that the method is effective.

Algorithm for predicting of the transition of a key point of geodynamic control to the risk zone

The article discusses the algorithm for assessing changes in the level of risk of karst hazard of the territory based on the forecasting of the number of holes depending on the water level. Based on the results of the analysis, the values of bifurcation parameters are determined, the transition through which sharply increases the formation of new holes. The article provides the developed block diagram of the neural network for assessing the dynamics of the occurrence of holes, as well as an algorithm for generating a predictive estimate of the number of holes. An analysis of the occurrence of the number of holes is carried out on the basis of water level data in the Oka River. The results of spline interpolation of the data are presented in the dependence on the number of holes on the dynamics of the water level in the river for the current and previous year. Practical verification of the developed algorithm was carried out on the basis of the new set of data on the water level in the river and the number of holes. The developed algorithm can be used in predicting the spread (leaching from the soil) of pollutants.

Effects of reducing chemical fertilizer on the quality components of Tieguanyin tea leaves

Fertilizer plays an important role in agricultural development. However, the soil environment of tea gardens has been deteriorated and the growth of tea trees has been hindered by the long-term excessive chemical fertilizer used in tea gardens. To study effects of reducing chemical fertilizer on the quality components of Tieguanyin tea leaves, Tieguanyin tea garden located in yellow-red soil was treated with three consecutive times and five recipes of chemical fertilizer. The results showed that appropriate reduction of chemical fertilizer applied in tea gardens can increase the contents of potassium and organic matter in soil, polyphenols and water extract in tea leaves. Most of the contents reach their peaks in T3 treatment, which compound fertilizer that the contents of NPK are all 15% was 412.50 kg/ha and urea was 206.25 kg/ha applied in the tea gardens, that 55% of the conventional usage by local farmers. At the same time, with the reducing chemical fertilizer applied in tea gardens, the caffeine in tea leaves was significantly decreased. Therefore, T3 treatment can improve the soil environment of tea gardens and the concentration of Oolong tea soup. Besides, it infers that the amount of chemical fertilizer in T3 treatment is a key control point for the quality of tea. The indirect influence coefficients that the chemical fertilizer effects on the contents of tea water extract, free amino acid and caffeine are all at their peaks of 5.249, 4.245 and 8.594 respectively through the changing of the soil nutrient contents. It is benefit for improving the overall quality of Oolong tea.

The effectiveness of payroll system in the public sector to prevent fraud

PurposeThe purpose of this paper is as follows: first, to analyze the opinion of the civil servant or payroll personnel towards the payroll system that currently used by the government. Second, to investigate the control measures currently exist in the payroll system and third, to examine any loopholes that may create opportunities for fraud to occur in that payroll system.Design/methodology/approachThis study involves a survey of questionnaires to the department’s personnel that act as respondents. One government department has been selected as a case study in this research. This study was focussing on the payroll administration unit that responsible for all personnel payroll operation and administration.FindingsThe study concludes that payroll system has equipped with the security and control characteristic. However, there is still a risk of fraud due to some internal control weaknesses in the system. Thus, the awareness about the control and security of the system should be given priority so that the staffs will understand and aware about it.Research limitations/implicationsThe study provides some evidence to indicate a weak and ineffective control measure in the payroll system that may lead to the fraud. Thus, by detail examination of the problems, the prevention and corrective action can be done in combating fraud. However, this study suffered from the small number of samples and limited access to the relevant documents due to confidentiality of the data.Practical implicationsThis study reveals that there are a few key internal control points that can be enhanced to facilitate better protection of payroll expenses in combating fraud in the government department. These include clear segregation of duties, stricter approval process and highly protected personnel information. In addition, adoption of whistleblowing channel, established internal audit function and severe punishment to the wrongdoers also can be implemented.Originality/valueThis study is original as it examines fraud and malpractices issues that specific for payroll department in the government department that rare in literature.

Allosteric Mechanism of the Hsp90 Chaperone Interactions with Cochaperones and Client Proteins by Modulating Communication Spines of Coupled Regulatory Switches: Integrative Atomistic Modeling of Hsp90 Signaling in Dynamic Interaction Networks.

Conformational landscapes of the Hsp90 chaperones have revealed that the intrinsic plasticity and functional adaptability of these molecular chaperones to a large cohort of cochaperones and a vast protein clientele can be regulated by a number of single switch points broadly dispersed in the chaperone structure. A detailed dynamic view of the allosteric changes mediated by conformational switches and the mechanism of their coupling during the ATPase cycle remains poorly understood and presents an important area of investigation. In this work, we employed integrative computational modeling that included evolutionary and coevolutionary analyses, experiment-guided protein docking and structure modeling, molecular simulations, energetic analysis, and network modeling to perform a systematic characterization of molecular and network signatures of conformational switch points and dissect their allosteric cross-talk in the Hsp90 complexes with cochaperones and client proteins. Using a hierarchical modeling of dynamic interaction networks, we show that the allosteric regulation of the Hsp90 interactions with p23 and Aha1 cochaperones and p53 client protein may be determined by the intramolecular communication "spines" of spatially separated and allosterically coupled regulatory switches. Using a battery of computational approaches, we examined how p23, Aha1, and p53 proteins can modulate signal transmission in the Hsp90 by exploiting communication spines of regulatory switches in the global allosteric network. This study proposes a community-chain mechanism of allosteric coupling between conformational switch centers and identifies key regulatory control points that mediate long-range interactions and communications in the Hsp90 chaperone. The results of this investigation provide novel insights into the nature of allosteric regulation mechanisms in the Hsp90 chaperones and offer a simple mechanistic model of the Hsp90 communications and adaptation to binding partners during the functional cycle.

The selection of parameters and control points in the geotechnical monitoring system

This article proposes an approach to the organization of the monitoring of geotechnical systems, which allows to increase the effectiveness of the automated control of destructive processes in geotechnical systems. The effectiveness of control is achieved by optimizing economic costs and technical constraints. The technical parameters of an automated geotechnical monitoring system are determined by the cost and importance of controlled objects. The criterion of optimality is the criterion of minimizing costs when implementing a geotechnical monitoring system with a constant value of possible damage. Possible damage in the geotechnical system during the development of destructive processes is expressed in monetary terms. The costs of implementing a geotechnical monitoring system are determined based on its cost, fixed costs for its maintenance, as well as technical parameters. The technical parameters in assessing the cost of implementing a geotechnical monitoring system are: the cost of measuring one controlled parameter, the number of measurement points, the cost of skipping the development of destructive geotechnical processes, the cost of a false alarm system. The proposed approach to optimizing the cost of implementing a geotechnical monitoring system is based on the monitoring method at key control points with a choice of bifurcation parameters. The proposed approach for optimizing the geotechnical monitoring system was tested in the city of Murom, Vladimir Region, and the Russian Federation while observing the development of suffusion processes of technogenic origin. The results of practical testing showed the possibility of reducing the cost of monitoring organization while maintaining control accuracy.

Hallmarks of Splicing Defects in Cancer: Clinical Applications in the Era of Personalized Medicine.

Alternative splicing promotes proteome diversity by using limited number of genes, a key control point of gene expression. Splicing is carried out by large macromolecular machineries, called spliceosome, composed of small RNAs and proteins. Alternative splicing is regulated by splicing regulatory cis-elements in RNA and trans-acting splicing factors that are often tightly regulated in a tissue-specific and developmental stage-specific manner. The biogenesis of ribonucleoprotein (RNP) complexes is strictly regulated to ensure that correct complements of RNA and proteins are coordinated in the right cell at the right time to support physiological functions. Any perturbations that impair formation of functional spliceosomes by disrupting the cis-elements, or by compromising RNA-binding or function of trans-factors can be deleterious to cells and result in pathological consequences. The recent discovery of oncogenic mutations in splicing factors, and growing evidence of the perturbed splicing in multiple types of cancer, underscores RNA processing defects as a critical driver of oncogenesis. These findings have resulted in a growing interest in targeting RNA splicing as a therapeutic approach for cancer treatment. This review summarizes our current understanding of splicing alterations in cancer, recent therapeutic efforts targeting splicing defects in cancer, and future potentials to develop novel cancer therapies.