Botulinum Toxin Type A (BoNT-A) injections are widely used for facial rejuvenation, but their effects on facial expressions remain unclear. This study aims to objectively measure the impact of BoNT-A injections on facial expressions using deep learning techniques. 180 patients aged 25-60 years who underwent BoNT-A application to the upper face were included. Patients were photographed with neutral, happy, surprised, and angry expressions before and 14 days after the procedure. A Convolutional Neural Network (CNN)-based Facial Emotion Recognition (FER) system analyzed 1440 photographs using a hybrid dataset of clinical images and the Karolinska Directed Emotional Faces (KDEF) dataset. The CNN model accurately predicted 90.15% of the test images. Significant decreases in the recognition of angry and surprised expressions were observed post-injection (p<0.05), with no significant changes in happy and neutral expressions (p>0.05). Angry expressions were often misclassified as neutral or happy (p<0.05), and surprised expressions were more likely to be perceived as neutral (p<0.05). Deep learning can effectively assess the impact of BoNT-A injections on facial expressions, providing more standardized data than traditional surveys. BoNT-A may reduce the expression of anger and surprise, potentially leading to a more positive facial appearance and emotional state. Further studies are needed to understand the broader implications of these changes.