Dynamic Translation Research Articles

Dynamic ranking and translation synchronization

Abstract In many applications, such as sport tournaments or recommendation systems, we have at our disposal data consisting of pairwise comparisons between a set of $n$ items (or players). The objective is to use these data to infer the latent strength of each item and/or their ranking. Existing results for this problem predominantly focus on the setting consisting of a single comparison graph $G$. However, there exist scenarios (e.g. sports tournaments) where the pairwise comparison data evolve with time. Theoretical results for this dynamic setting are relatively limited, and are the focus of this paper. We study an extension of the translation synchronization problem, to the dynamic setting. In this set-up, we are given a sequence of comparison graphs $(G_t)_{t\in{{\mathscr{T}}}}$, where $ {{\mathscr{T}}} \subset [0,1]$ is a grid representing the time domain, and for each item $i$ and time $t\in{{\mathscr{T}}}$ there is an associated unknown strength parameter $z^*_{t,i}\in{{\mathbb{R}}}$. We aim to recover, for $t\in{{\mathscr{T}}}$, the strength vector $z^*_t=(z^*_{t,1},\dots ,z^*_{t,n})$ from noisy measurements of $z^*_{t,i}-z^*_{t,j}$, where $\left \{{i,j}\right \}$ is an edge in $G_t$. Assuming that $z^*_t$ evolves smoothly in $t$, we propose two estimators—one based on a smoothness-penalized least squares approach and the other based on projection onto the low-frequency eigenspace of a suitable smoothness operator. For both estimators, we provide finite sample bounds for the $\ell _2$ estimation error under the assumption that $G_t$ is connected for all $t\in{{\mathscr{T}}}$, thus proving the consistency of the proposed methods in terms of the grid size $|\mathscr{T}|$. We complement our theoretical findings with experiments on synthetic and real data.

Post-transcriptional regulation of erythropoiesis.

Erythropoiesis is a complex, precise, and lifelong process that is essential for maintaining normal body functions. Its strict regulation is necessary to prevent a variety of blood diseases. Normal erythropoiesis is precisely regulated by an intricate network that involves transcription levels, signal transduction, and various epigenetic modifications. In recent years, research on post-transcriptional levels in erythropoiesis has expanded significantly. The dynamic regulation of splicing transitions is responsible for changes in protein isoform expression that add new functions beneficial for erythropoiesis. RNA-binding proteins adapt the translation of transcripts to the protein requirements of the cell, yielding mRNA with dynamic translation efficiency. Noncoding RNAs, such as microRNAs and lncRNAs, are indispensable for changing the translational efficiency and/or stability of targeted mRNAs to maintain the normal expression of genes related to erythropoiesis. N6-methyladenosine-dependent regulation of mRNA translation plays an important role in maintaining the expression programs of erythroid-related genes and promoting erythroid lineage determination. This review aims to describe our current understanding of the role of post-transcriptional regulation in erythropoiesis and erythroid-associated diseases, and to shed light on the physiological and pathological implications of the post-transcriptional regulation machinery in erythropoiesis. These may help to further enrich our understanding of the regulatory network of erythropoiesis and provide new strategies for the diagnosis and treatment of erythroid-related diseases.

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis

Germinal center (GC) B cells undergo proliferation at very high rates in a hypoxic microenvironment but the cellular processes driving this are incompletely understood. Here we show that the mitochondria of GC B cells are highly dynamic, with significantly upregulated transcription and translation rates associated with the activity of transcription factor A, mitochondrial (TFAM). TFAM, while also necessary for normal B cell development, is required for entry of activated GC precursor B cells into the germinal center reaction; deletion of Tfam significantly impairs GC formation, function and output. Loss of TFAM in B cells compromises the actin cytoskeleton and impairs cellular motility of GC B cells in response to chemokine signaling, leading to their spatial disorganization. We show that B cell lymphoma substantially increases mitochondrial translation and that deletion of Tfam in B cells is protective against the development of lymphoma in a c-Myc transgenic mouse model. Finally, we show that pharmacological inhibition of mitochondrial transcription and translation inhibits growth of GC-derived human lymphoma cells and induces similar defects in the actin cytoskeleton.

A Burglar of the Better Sort: Poems, Dramatic Works, Theoretical Writings

A Burglar of the Better Sort: Poems, Dramatic Works, Theoretical Writings

180 FDA Trial of Decompression and Dynamic Sagittal Tether for Degenerative Spondylolisthesis: 24-Months Clinical and Radiographic Follow-Up

INTRODUCTION: Degenerative spondylolisthesis (DS) with lumbar spinal stenosis (LSS) is commonly treated with decompression and fusion. The LimiFlex Dynamic Sagittal Tether (DST) is an investigational stabilization device for patients with DS and LSS. METHODS: Patients with single-level Grade 1 DS with LSS were enrolled in FDA-IDE study (NCT03115983) comparing decompression with DST and decompression with TLIF. Clinical and radiographic outcomes were assessed at baseline through 24mo postop. All propensity score (PS) selected patients who had 24mo follow-up were included in this interim analysis. RESULTS: At 24 mo, 224 PS-selected patients (125DST/99TLIF) had clinical follow-up. Mean characteristics of DST/TLIF groups were: age 65.8/64.1 yrs; BMI 28.1/30.0; CCI 2.5/2.4; and 57.9%/70.1% female. Mean perioperative outcomes for DST/TLIF were: procedure time 112/187 min; EBL 52/240mL; LOS 0.6/2.9 d. Mean improvements at 24mo for the DST/TLIF groups were -58.0/-57.5 mm VAS-leg/hip, -48.3/-42.2 VAS-back and -40.0/-33.4 ODI with 91%/83% achieving 15pt ODI improvement. Within 24 months, 6.5% of DST and 7.6% of TLIF patients had additional surgery at the index or adjacent level. Radiographically, at 24mo, mean reductions for the DST/TLIF groups were -1.7°/-3.7° in flexion/extension ROM and -0.4 mm/-1.0 mm in dynamic translation. At baseline/24mo, the index segment accounted for 30%/27% of index+adjacent segments ROM in the DST group and 29%/12% in the TLIF group. CONCLUSIONS: These results suggest that decompression+DST stabilization for DS can achieve significant clinical improvement like fusion, without an increase in reoperations within 24 mo. Imaging demonstrated no increased instability in the DST group with maintained distribution of motion between the index+adjacent segments. Further follow-up should include PS-adjusted analysis of the study composite clinical success and longer follow-up to demonstrate clinical durability of this treatment.

Facet Articular Irregularity Is the Most Relevant Risk Factor for Rapidly Progressive Degenerative Cervical Myelopathy.

Facet articular irregularity is associated with rapidly progressive degenerative cervical myelopathy (DCM). However, its significance compared with other known risk factors remains unknown. Therefore, this retrospective study aimed to clarify the potential impact of facet articular irregularity as a risk factor for rapid DCM progression. This study included 141 consecutive patients with DCM who underwent surgical treatment at our institution. Clinical variables and radiological findings related to DCM progression were collected. Imaging findings were analyzed at the segmental level of myelopathy in each case. The patients were divided into 2 groups based on the presence or absence of rapid DCM progression, and independent risk factors were determined using logistic regression analyses. Overall, 131 patients with a mean age of 63.9 ± 12.6 years were analyzed; 27 patients (20.6%) were classified into the rapid DCM progression group. The mean age was significantly higher in the rapid progression group than in the slow progression group (72.4 ± 9.6 vs. 61.7 ± 12.4, p < 0.001). According to univariate analysis, facet articular irregularity, dynamic segmental translation (≥ 1.6 mm), upper cervical spine involvement above C4-5, history of cerebrovascular events, preceding minor trauma, local lordotic angle (≥ 4.5°), diabetes, hypertension, ligamentum flavum hypertrophy, and age were independent risk factors. Additionally, multivariate analysis showed that facet articular irregularity was the highest risk factor for rapid DCM progression (p = 0.001). Facet articular irregularity is the most clinically significant finding among the known risk factors in patients with rapid DCM progression.

Aminoacyl-tRNA Synthetase Evolution within the Dynamic Tripartite Translation System of Plant Cells.

Eukaryotes maintain separate protein translation systems for nuclear and organellar genes, including distinct sets of tRNAs and aminoacyl-tRNA synthetases (aaRSs). In animals, mitochondrial-targeted aaRSs are expressed at lower levels and are less conserved in sequence than cytosolic aaRSs involved in translation of nuclear mRNAs, likely reflecting lower translational demands in mitochondria. In plants, translation is further complicated by the presence of plastids, which share most aaRSs with mitochondria. In addition, plant mitochondrial tRNA pools have a dynamic history of gene loss and functional replacement by tRNAs from other compartments. To investigate the consequences of these distinctive features of translation in plants, we analyzed sequence evolution in angiosperm aaRSs. In contrast to previously studied eukaryotic systems, we found that plant organellar and cytosolic aaRSs exhibit only a small difference in expression levels, and organellar aaRSs are slightly more conserved than cytosolic aaRSs. We hypothesize that these patterns result from high translational demands associated with photosynthesis in mature chloroplasts. We also investigated aaRS evolution in Sileneae, an angiosperm lineage with extensive mitochondrial tRNA replacement and aaRS retargeting. We predicted positive selection for changes in aaRS sequence resulting from these recent changes in subcellular localization and tRNA substrates but found little evidence for accelerated sequence divergence. Overall, the complex tripartite translation system in plant cells appears to have imposed more constraints on the long-term evolutionary rates of organellar aaRSs compared with other eukaryotic lineages, and plant aaRS protein sequences appear largely robust to more recent perturbations in subcellular localization and tRNA interactions.

Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.

Translation is a key determinant of gene expression and an important biotechnological engineering target. In bacteria, 5'-untranslated region (5'-UTR) and coding sequence (CDS) are well-known mRNA parts controlling translation and thus cellular protein levels. However, the complex interaction of 5'-UTR and CDS has so far only been studied for few sequences leading to non-generalisable and partly contradictory conclusions. Herein, we systematically assess the dynamic translation from over 1.2 million 5'-UTR-CDS pairs in Escherichia coli to investigate their collective effect using a new method for ultradeep sequence-function mapping. This allows us to disentangle and precisely quantify effects of various sequence determinants of translation. We find that 5'-UTR and CDS individually account for 53% and 20% of variance in translation, respectively, and show conclusively that, contrary to a common hypothesis, tRNA abundance does not explain expression changes between CDSs with different synonymous codons. Moreover, the obtained large-scale data provide clear experimental evidence for a base-pairing interaction between initiator tRNA and mRNA beyond the anticodon-codon interaction, an effect that is often masked for individual sequences and therefore inaccessible to low-throughput approaches. Our study highlights the indispensability of ultradeep sequence-function mapping to accurately determine the contribution of parts and phenomena involved in gene regulation.

The Reliability of Acromioclavicular Joint Dislocation Classification Systems: A Comparison Between the Rockwood and Kraus Classifications.

The Rockwood system for the classification of acute acromioclavicular (AC) joint dislocations has been associated with a lack of reliability. A novel system has been proposed (Kraus classification) that is based on dynamic posterior translation of these injuries. To assess the interobserver and intraobserver reliability of the Rockwood and Kraus classification systems and also to examine the impact of surgeon experience on the assessments. Cohort study (diagnosis); Level of evidence, 3. Included were 45 patients with acute AC joint injuries who underwent a radiographic examination using standard bilateral AP and modified Alexander views. For interobserver reliability, 6 shoulder surgeons (expert group) and 6 orthopaedic residents (novice group) reviewed the radiographs to classify injuries according to the Rockwood and Kraus classifications; for each categorization, the participants chose between nonoperative management and surgical treatment. The evaluation was repeated 6 weeks later to determine intraobserver reliability. Kappa (κ) coefficients and their 95% CIs were used to compare interobserver and intraobserver reliability for each classification system according to assessor experience. Statistical differences were considered significant when the upper and lower boundaries of the 95% CI did not overlap. The overall interobserver agreement for diagnosis (both novice and expert groups) was moderate (κ = 0.52 [95% CI, 0.51-0.54]) for the Rockwood classification and substantial (κ = 0.62 [95% CI, 0.53-0.65]) for the Kraus classification; however, no significant differences were observed between the κ values. The overall interobserver agreement for treatment selection was substantial when using both the Rockwood (κ = 0.78 [95% CI, 0.72-0.81]) and Kraus (κ = 0.77 [95% CI, 0.66-0.87]) classifications. The overall intraobserver agreement for diagnosis was substantial using both the Rockwood (κ = 0.65 [95% CI, 0.61-0.67]) and Kraus (κ = 0.73 [95% CI, 0.69-0.75]) classifications. There were no significant differences between the novice and expert groups on any of the evaluations. The Kraus system was slightly more reliable than the Rockwood system for classifying AC joint injuries both between assessor groups and overall. The level of surgeon experience had no impact on the evaluations.

Formally Verified Native Code Generation in an Effectful JIT: Turning the CompCert Backend into a Formally Verified JIT Compiler

Modern Just-in-Time compilers (or JITs) typically interleave several mechanisms to execute a program. For faster startup times and to observe the initial behavior of an execution, interpretation can be initially used. But after a while, JITs dynamically produce native code for parts of the program they execute often. Although some time is spent compiling dynamically, this mechanism makes for much faster times for the remaining of the program execution. Such compilers are complex pieces of software with various components, and greatly rely on a precise interplay between the different languages being executed, including on-stack-replacement. Traditional static compilers like CompCert have been mechanized in proof assistants, but JITs have been scarcely formalized so far, partly due to their impure nature and their numerous components. This work presents a model JIT with dynamic generation of native code, implemented and formally verified in Coq. Although some parts of a JIT cannot be written in Coq, we propose a proof methodology to delimit, specify and reason on the impure effects of a JIT. We argue that the daunting task of formally verifying a complete JIT should draw on existing proofs of native code generation. To this end, our work successfully reuses CompCert and its correctness proofs during dynamic compilation. Finally, our prototype can be extracted and executed.

Mitigating International Norms Through Contestation: Peaceful Purposes in Space Law and the Security Dilemma

The present contribution proposes to analyse the evolution of the meaning of ‘peaceful’ in space law as an example of how international norms can be emptied of their substance without putting their legality into question. By highlighting different phases of the evolution of the ‘peaceful purposes’ principle and linking it to the peculiar context and security dilemmas of the time, the motivations and the form in which the principle was contested are fleshed out. Drawing on this case study of legislative contestation, it concludes that in matters related to international security, security dilemmas can shape subsequent State practice, allow for a translation of the power dynamics of the international community into international law, and lead to the erosion of norms. In these circumstances, the importance given to the subsequent practice of the states should be reduced in relation to the other interpretive tools.

A non-weight bearing protocol after ACL reconstruction improves static anterior tibial translation in patients with elevated slope and increased weight bearing tibial anterior translation

PurposeAim of this study is to evaluate the impact of a non-weight bearing (NWB) protocol within 21 post-operative days after anterior cruciate ligament (ACL) reconstruction on static and dynamic anterior tibial translations (SATT and DATT, respectively). The hypothesis is that delayed WB would improve ATT at 9 months follow-up.MethodsA series of patients treated with ACL reconstruction was retrospectively reviewed, comparing a group with immediate post-operative weight bearing (WB group) and a group without post-operative weight bearing (NWB group). The NWB protocol was applied to patients with posterior tibial slope (PTS) ≥ 12°, pre-operative SATT ≥ 5 mm, and/or meniscal lesions of root or radial type. SATT, and PTS were measured on 20° flexion monopodal lateral x-rays, while DATT on Telos™ x-rays at pre-operative and 9-months follow-up.ResultsOne hundred seventy-nine patients were included (50 NWB group, 129 WB group). The SATT worsened in the WB group with a mean increase of 0.7 mm (SD 3.1 mm), while in the NWB group, the SATT improved with a mean decrease of 1.4 mm (SD 3.1 mm) from the pre-operative to 9 months’ follow-up (p < 0.001). The side-to-side Telos™ evaluation showed a significant improvement in DATT within both the groups (p < 0.001), but there was no difference between the two groups (p = 0.99).ConclusionThe post-operative protocol of 21 days without WB led to an improvement in SATT at 9 months without an influence on DATT, and it is recommended for patients with a SATT ≥ 5 mm and/or a PTS ≥ 12° as part of an “à la carte” approach to ACL reconstruction.Level of evidenceLevel IV, Retrospective case series

Stemness‐related genes revealed by single‐cell profiling of naïve and stimulated human CD34+ cells from CB and mPB

Hematopoietic stem cells (HSCs) from different sources show varied repopulating capacity, and HSCs lose their stemness after long-time ex vivo culture. A deep understanding of these phenomena may provide helpful insights for HSCs. Here, we applied single-cell RNA-seq (scRNA-seq) to analyse the naïve and stimulated human CD34+ cells from cord blood (CB) and mobilised peripheral blood (mPB). We collected over 16 000 high-quality single-cell data to construct a comprehensive inference map and characterised the HSCs under a quiescent state on the hierarchy top. Then, we compared HSCs in CB with those in mPB and HSCs of naïve samples to those of cultured samples, and identified stemness-related genes (SRGs) associated with cell source (CS-SRGs) and culture time (CT-SRGs), respectively. Interestingly, CS-SRGs and CT-SRGs share genes enriched in the signalling pathways such as mRNA catabolic process, translational initiation, ribonucleoprotein complex biogenesis and cotranslational protein targeting to membrane, suggesting dynamic protein translation and processing may be a common requirement for stemness maintenance. Meanwhile, CT-SRGs are enriched in pathways involved in glucocorticoid and corticosteroid response that affect HSCs homing and engraftment. In contrast, CS-SRGs specifically contain genes related to purine and ATP metabolic process, which is crucial for HSC homeostasis in the stress settings. Particularly, when CT-SRGs are used as reference genes for the construction of the development trajectory of CD34+ cells, lymphoid and myeloid lineages are clearly separated after HSCs/MPPs. Finally, we presented an application through a small-scale drug screening using Connectivity Map (CMap) against CT-SRGs. A small molecule, cucurbitacin I, was found to efficiently expand HSCs ex vivo while maintaining its stemness. Our findings provide new perspectives for understanding HSCs, and the strategy to identify candidate molecules through SRGs may be applicable to study other stem cells.

RegCPython: A Register-based Python Interpreter for Better Performance

Interpreters are widely used in the implementation of many programming languages, such as Python, Perl, and Java. Even though various JIT compilers emerge in an endless stream, interpretation efficiency still plays a critical role in program performance. Does a stack-based interpreter or a register-based interpreter perform better? The pros and cons of the pair of architectures have long been discussed. The stack architecture is attractive for its concise model and compact bytecode, but our study finds that the register-based interpreter can also be implemented easily and that its bytecode size only grows by a small margin. Moreover, the latter turns out to be appreciably faster. Specifically, we implemented an open source Python interpreter named RegCPython based on CPython v3.10.1. The former is register based, while the latter is stack based. Without changes in syntax, Application Programming Interface, and Application Binary Interface, RegCPython is excellently compatible with CPython, as it does not break existing syntax or interfaces. It achieves a speedup of 1.287 on the most favorable benchmark and 0.977 even on the most unfavorable benchmark. For all Python-intensive benchmarks, the average speedup reaches 1.120 on x86 and 1.130 on ARM. Our evaluation work, which also serves as an empirical study, provides a detailed performance survey of both interpreters on modern hardware. It points out that the register-based interpreters are more efficient mainly due to the elimination of machine instructions needed, while changes in branch mispredictions and cache misses have a limited impact on performance. Additionally, it confirms that the register-based implementation is also satisfactory in terms of memory footprint, compilation cost, and implementation complexity.

LibRoadRunner 2.0: a high performance SBML simulation and analysis library.

This article presents libRoadRunner 2.0, an extensible, high-performance, cross-platform, open-source software library for the simulation and analysis of models expressed using the systems biology markup language (SBML). libRoadRunner is a self-contained library, able to run either as a component inside other tools via its C++, C and Python APIs, or interactively through its Python or Julia interface. libRoadRunner uses a custom just-in-time (JIT) compiler built on the widely used LLVM JIT compiler framework. It compiles SBML-specified models directly into native machine code for a large variety of processors, making it fast enough to simulate extremely large models or repeated runs in reasonable timeframes. libRoadRunner is flexible, supporting the bulk of the SBML specification (except for delay and non-linear algebraic equations) as well as several SBML extensions such as hierarchical composition and probability distributions. It offers multiple deterministic and stochastic integrators, as well as tools for steady-state, sensitivity, stability and structural analyses. libRoadRunner binary distributions for Windows, Mac OS and Linux, Julia and Python bindings, source code and documentation are all available at https://github.com/sys-bio/roadrunner, and Python bindings are also available via pip. The source code can be compiled for the supported systems as well as in principle any system supported by LLVM-13, such as ARM-based computers like the Raspberry Pi. The library is licensed under the Apache License Version 2.0.

Relevant mathematical modelling efforts for understanding COVID-19 dynamics: an educational challenge.

The online version contains supplementary material available at 10.1007/s11858-022-01447-2.

Excalibur

In recent years, hardware has become increasingly diverse, in terms of features as well as performance. This poses a problem for complex software in general and database systems in particular. To achieve top-notch performance, we need to exploit hardware features, but do not fully know which behave best on the current, and more-so future, machines. Specializing query execution methods for many diverse hardware platforms will significantly increase database software complexity and also poses a physical query optimization problem that cannot be solved robustly with static cost models. In this paper, we propose a new query execution architecture addressing these problems. Based on the flexible domain-specific language VOILA, it can generate thousands of different flavors from a single code-base. As an abstraction, a virtual machine (VM) allows hiding physical execution details, such that the VM can transparently switch between different execution tactics within each query, applied at a fine granularity. We show rules to describe a search space for good tactics, and describe efficient search strategies, that limit the overhead of adaptive JIT code generation and compilation. The VM starts executing each query in full vectorized code style, but adaptively replaces (parts of) query pipelines by code fragments compiled using different execution flavors, exploring this search space and exploiting the best tactics found, casting adaptive query execution into a Multi-Armed Bandit (MAB) problem. Excalibur, our prototype, outperforms open-source systems by up to 28X and the state-of-the-art system Umbra by up to 1.8X. In specific queries Excalibur performs up to 2X faster than static flavors.

LuisaRender

The advancements in hardware have drawn more attention than ever to high-quality offline rendering with modern stream processors, both in the industry and in research fields. However, the graphics APIs are fragmented and existing shading languages lack high-level constructs such as polymorphism, which adds complexity to developing and maintaining cross-platform high-performance renderers. We present LuisaRender 1 , a high-performance rendering framework for modern stream-architecture hardware. Our main contribution is an expressive C++-embedded DSL for kernel programming with JIT code generation and compilation. We also implement a unified runtime layer with resource wrappers and an optimized Monte Carlo renderer. Experiments on test scenes show that LuisaRender achieves much higher performance than existing research renderers on modern graphics hardware, e.g., 5--11× faster than PBRT-v4 and 4--16× faster than Mitsuba 3.

Rendering of the Verbs and Verbals of Saying in the Georgian Versions of the Books of the Minor Prophets

It is well known that the Septuagint&nbsp; Greek is characterized by the use of more than one (as a rule, two) verbs and verbals (participles) of saying before the direct speech, which is a Hebraism,&nbsp; for instance: καὶ ἀπεκρίθη Αμως καὶ εἶπεν πρὸς Αμασιαν …] Then answered Amos, and said to Amaziah… [Amos 7:14]. &nbsp;The aim of the given paper is to analyze the issue of rendering of the verbs and participles of saying into Georgian. With this aim, I have compared the old Georgian translation/edition (X-XI cc.) of all the twelve books of Minor Prophets with the Georgian version of the Hellenophile period (XII –XIII cc.). Observation of the Georgian versions of the Bible has proved that the earlier translator takes more account of the nature of the Georgian language. Therefore, the old versions of the Bible occupy a somewhat intermediate position between the formal and dynamic translations, whereas the translator-revisor of the Hellenophile period tries hard to preserve formal-structural or semantic equivalence with the Greek language.

The Influence of Surgical Realignment Procedures on Dynamic Patellar Tracking: A Dynamic Magnetic Resonance Imaging-Controlled Feasibility Study.

Persisting patellar maltracking following surgical realignment often remains unseen. The aim of this study was to analyze the effects of realignment procedures on patellofemoral kinematics in patients with patellofemoral instability (PFI) and patellofemoral maltracking (PM) by using dynamic magnetic resonance imaging (MRI). Patients planned for surgical patellar realignment due to PFI and a clinically and radiologically apparent PM between December 2019 and May 2022 were included. Patients without PM, limited range of motion, joint effusion, or concomitant injuries were excluded. Dynamic mediolateral translation (dMPT) and patella tilt (dPT) were measured preoperatively and three months postoperatively. In 24 patients (7 men, 17 women; mean age 23.0 years), 10 tibial tubercle transfers, 5 soft tissue patella tendon transfers, 6 trochleoplasties, 3 lateral lengthenings, 1 varizating distal femoral osteotomy (DFO), and 1 torsional DFO were performed. At final follow-up, dMPT (from 10.95 ± 5.93 mm to 4.89 ± 0.40 mm, p &lt; 0.001) and dPT (from 14.50° ± 10.33° to 8.44° ± 7.46°, p = 0.026) were significantly improved. All static radiological parameters were corrected to physiological values. Surgical patellar realignment contributed to the significant improvement of patellofemoral kinematics, with an approximation to normal values. The postoperative application of dynamic MRI allowed for a quantification of the performed correction, allowing for a postoperative control of success.