Juice Color Research Articles

Clarification of pomegranate and strawberry juices: Effects of various clarification agents on turbidity, anthocyanins, colour, phenolics and antioxidant activity

Effects of clarification on turbidity, anthocyanins (ACNs), colour, phenolics, ascorbic acid and antioxidant activity (AA) of pomegranate (PJ) and strawberry juices (SJ) were investigated. Cold-clarification was applied to PJ using only gelatine. For hot-clarification of both juices, bentonite, gelatine and kieselsol were used. Moreover, pectolytic enzyme was added to SJ that contains pectin. Depectinization caused an increase in colour density of SJ and bentonite treatment resulted in low turbidity (9.5 NTU). However, gelatine and kieselsol significantly reduced ACN content (15%) and increased turbidity (70.1 NTU). In PJ, no step during hot-clarification provided low turbidity (93.2–162.0 NTU), while relatively low turbidity (28.7 NTU) and anthocyanin loss (9.8%) was achieved during cold-clarification. Hot-clarification of SJ showed no effect on AA and phenolics, while cold-clarification of PJ reduced both to a limited extent. Therefore, depectinization and bentonite treatment are recommended for hot-clarification of SJ and gelatine for cold-clarification of PJ.

Different Treatments for Sugarcane Juice Preservation.

This investigation aimed to optimize the time, pH, pressure, and temperature of sugarcane juice pasteurization and to develop a "ready to serve" bottled sugarcane juice with a high preservation efficiency. Fresh sugarcane juice was extracted from sugarcane genotype Co 89003, and beverage samples were collected using three different treatments: sulphitation of juice with the addition of potassium metabisulphite (KMS-25, 50, 100, and 150 ppm), acidification of juice (addition of citric acid, to reduce the pH of the juice to 4.8, 4.5, and 4.25), and steam treatment of the canes (5 min, 10, and 15 min at 7 psi). In all treatments, the juice was pasteurized in glass bottles @ 65 °C for 25 min and stored at low temperature (5 °C) in pre-sterilized glass bottles. Juice properties such as the ˚Brix, total sugar, pH, and total phenolic content decreased with storage, whereas the microbial count, titrable acidity, and reducing sugar content significantly increased during storage. The addition of KMS, citric acid, and the steam treatment reduced the browning of juice and maintained the color of juice during storage, by inhibiting the polyphenol oxidase enzyme activity, from 0.571 unit/mL to 0.1 unit/mL. Among the selected treatments, sugarcane juice with KMS (100 and 150 ppm) and steam treatment of the canes for 5 and 10 min at 7 psi showed the minimum changes in physico-chemical properties, sensory qualities, and restricted microbial growth. Thesulphitation treatment with pasteurization proved best for increasing the shelf life of sugarcane juice upto 90 days with refrigeration. Similarly, the steam-subjected cane juice (10 and 15 min at 7 psi) could be effectively preserved for upto 30 days with refrigeration, without any preservative.

Componentes de rendimento e atributos físicos da videira ‘BRS Magna’ sobre diferentes porta-enxertos

ABSTRACT ‘BRS Magna’ was released with the aim of improving the colour, sweetness, and flavour of Brazilian grape juice. Therefore, it is necessary to test this cultivar on different rootstocks in order to identify the canopy/rootstock combinations that best contribute to the purpose of the enterprise under cultivation conditions. Thus, the objective of this study was to evaluate the influence of different rootstocks on the yield components and physical characteristics of the bunch and berry of the ‘BRS Magna’ grapevine. The research was conducted in the vineyard established in the experimental area of the Universidade Tecnológica Federal do Paraná (UTFPR), located in the municipality of Pato Branco, Paraná. The ‘BRS Magna’ was grafted onto 10 rootstocks: ‘Kober 5BB’, ‘SO4’, ‘IAC 766 Campinas’, ‘Harmony’, ‘Paulsen 1103’, ‘420A’, ‘IAC 572 Jales’, ‘Freedom’, ‘IAC 313 Tropical’, and ‘101-14 MGT’. For three agricultural years, production, productivity, vegetative vigor, number of bunches, and average bunch weight per plant, length and width of bunch and berry, as well as average berry weight were evaluated. Analysis of variance showed a interaction between rootstocks and production years for all variables. ‘BRS Magna’ showed alternation in yield components, vegetative vigor and physical characteristics of bunch and berry between the evaluated crops. Under the cultivation conditions studied, the recommended rootstock for the ‘BRS Magna’ cultivar is ‘IAC 572 Jales’. On the other hand, ‘101-14 MGT’ and ‘420 A’ are not recommended as rootstock for this cultivar.

Industry-Scale Microfluidizer: a Novel Technology to Improve Physiochemical Qualities and Volatile Flavor of Whole Mango Juice

Industry-scale microfluidizer system (ISMS) is a novel equipment for producing filtered-free whole beverages. This study investigated the color, nutrition, and flavor changes of whole mango juice (WMJ) treated by ISMS under various pressure (0–120 MPa). Results showed that ISMS well maintained the total soluble solid content, pH value, and yellow color of WMJ. The carotenoids were not destroyed with treatment pressure below 90 MPa, and the carotenoids bioaccessibility of all the WMJ samples was no change. More importantly, ISMS promoted the release of ascorbic acid and total polyphenols by disrupting cellular integrity, thus improving antioxidant activities of WMJ. Volatile compounds analysis found that WMJ samples had more terpenes and aldehydes when treated at 90 MPa, and possessed more esters at 120 MPa. These results demonstrated that ISMS might offer new opportunities to produce WMJ with excellent qualities at an industrial level.

Characterization of fruit juices and effect of pasteurization and storage conditions on their microbial, physicochemical, and nutritional quality

Characterization, pasteurization and storage are essential steps in fruit juice processing. Watermelon, pineapple, and mango juices were pasteurized at 80 ± 2 °C and held at different treatment times (1, 2.5, 5, 10, and 15 min). Juice yield, pH, proximate composition, total soluble solids, color, vitamin C, microbial quality, mineral content, enzyme activity (polyphenol oxidase (PPO), and peroxidase (POD)), total phenolic content, and antioxidant capacity were measured during pasteurization and cold storage (4 °C). Results showed that watermelon juice had the highest crude protein, pH, and moisture content, pineapple juice had the highest titratable acidity, vitamin C and mineral content (potassium, calcium, magnesium, manganese, and zinc) and mango juice had the highest juice yield, and total soluble solids. Regardless of the holding time, pasteurization reduced total plate counts and yeast and molds to below detectable limits (1 log CFU/mL). Vitamin C was undetectable in watermelon juice after 10 min of pasteurization compared to mango juice with a 27% reduction. Pasteurization preserved mango juice color, but watermelon juice became less red and more yellow with increasing treatment time. POD was more thermoresistant than PPO and needed a treatment time of at least 5 min to obtain 80% reduction. Storage of more than 9 days negatively affected the watermelon color, total phenolic content and antioxidant capacities of watermelon juice pasteurized at 15 min and vitamin C content of unpasteurized mango juice. Thus, pasteurization and storage affect fruit juice quality depending on the type of fruit and their composition.

Physicochemical, antioxidant capacity, and sensory properties of bligo juice (Benicasa hispida) with bligo peel extract addition

This study aimed to develop bligo juice as functional food by adding Bligo Peel Extract (BPE) with concentrations of 1%, 2%,and 3%. The characteristics of bligo juice before and after BPE addition were investigated by proximate analysis, examination of total solids, viscosity, vitamin C, and antioxidant capacity, and sensory evaluation. Results showed that BPE addition increased the vitamin C levels, antioxidant activity, carbohydrates, fats, and acceptance of color, flavor, and viscosity of bligo juice but decreased its acceptance of taste and acceptability. The addition of 1% BPE provided the highest acceptance rate compared with other variations. In summary, BPE addition increases the vitamin C, antioxidant activity, and carbohydrate content of bligo juice, thus improving its health benefits.

Study on the mechanism of interaction between mulberry anthocyanins and yeast mannoprotein

To solve the problem of mulberry juice colour instability caused by ultraviolet radiation, we proposed the addition of mannoprotein (MP) to improve the stabilization of mulberry juice colour. First, the purified mulberry anthocyanins extracted (MAE) were identified by UFLC–MS, and their purity was determined by liquid-phase peak area normalization. The interaction mechanism between MP and MAE at different pH values was systematically investigated by Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, and molecular docking. After conjugation with MAE, the secondary structure of MP changed slightly. The fluorescence quenching of MP by MAE was dose dependent. Thermodynamic analysis showed that the main interaction forces at pH 3.2 were hydrogen bonding and van der Waals forces, while the main force at pH 7.4 was hydrophobic forces. The molecular docking results verified the good binding properties of MP with cyanidin-3-O-glucoside (C3G) and cyanidin-3-O-rutinoside (C3R).

Impact of the Anodic and Cathodic Electro-Activation Treatment on the Physico-Chemical and Antioxidant Capacity of Red Beetroot Juice

The aim of the present work was to study the feasibility of using electro-activation as a nonthermal treatment to produce stable beetroot juice. Specifically, red beetroot juice was electro-activated under two different reactor configurations by using three electric current intensities (100, 200, and 300 mA) during 120 min. Different parameters of the juice were measured such as the pH, redox potential, juice titratable acidity, Brix degree and total dry matter, color, betalain and polyphenolic contents, and antioxidant capacity of the electro-activated juice. By using the reactor Configuration A in which the targeted juice was electro-activated in the anodic compartment of the used reactor, acidic juice with pH 4 and 5 as well as a redox potential close to +300 mV was obtained. The Brix degree, color, dry matter, and phenolic content were not significantly influenced by this electro-activation. However, the treatment permitted increasing the antioxidant capacity of the juice as measured by the DPPH and ABTS assays. By using the reactor Configuration B in which the targeted beet juice was electro-activated in the cathodic compartment of the used compartment, a juice with an alkaline pH of approximately pH 9 and a reducing redox potential of -697 mV was obtained. With this reactor configuration, the Brix degree and total dry matter were not affected, but the color and total polyphenolic content changed. The betalains and polyphenolic compounds were degraded under the alkaline conditions of this electro-activation treatment, which had a negative consequence on the juice quality by decreasing its antioxidant capacity. In conclusion, this study demonstrated that anodic electro-activation of a beet juice can be technologically feasible since this treatment permitted producing stable juice as well as maintaining the main physico-chemical properties of the juice, enhancing its antioxidant capacity, and keeping the juice color at high level.

THE EFFECT OF SULFITATION TREATMENT OF BEET-SUGAR PRODUCTION SEMI-PRODUCTS ON THE PROCESS OF INTENSIVELY COLORED HIGH MOLECULAR COMPOUNDS INHIBITION

Studies have been carried out to identify the effect of sulfitation treatment of sugar-beet processing semi-products on their color when it is carried out at one or several processing stages, as well as in its complete absence. At the first stage, the influence of the process of sulfitation treatment of the extractant and thin juice on the color of the thin juice and thick juice obtained from the thin juice by evaporation was studied. It was established that the sulfitation treatment of only the extractant provided a decrease in the color of the thick juice by 9.21%, while the sulfitation treatment of only thin juice – by 55.00%, and the sulfitation treatment of extractant and thin juice – by 58.16%. In addition, an increase in the purity of the thick juice by 0.45% due to the sulfitation treatment of the extractant and thin juice reduced the sucrose content in molasses by 0.11% and, consequently, reduced the overall loss of sugar in production. At the second stage, the effect of sulfitation treatment of a B+C remelt syrups with the use of sulfur dioxide and sodium bisulfite was studied. It was established that the amount of blocked aldehyde and ketone groups when thick juice was treated with sulfur dioxide was higher than when treated with sodium bisulfite due to the divalent anion SO 3 2– , and not the monovalent HSO 3– . At the third stage, under production conditions, the effect of sulfitation treatment of massecuite I on the boiling time and color of white sugar was studied. It was established that the average value of the color of white sugar in the presence of sulfitation treatment of massecuite I was 0.44 ICUMSA units, while without treatment – 0.50 ICUMSA units, i.e. under production conditions, the sulfitation treatment of massecuite A made it possible to reduce the color of the finished product – white sugar by 12.0%.

Effects of different maceration techniques on the colour, polyphenols and antioxidant capacity of grape juice

This study investigated the effects of different maceration techniques on the colour parameters, phenolic content and antioxidant activity of grape juice. Maceration techniques influenced colour parameters, and a* and Hue ranged from −0.77 to 0.55 and 60.90 to 104.40, respectively. The microwave and microwave and sonication combination increased the total monomeric anthocyanin, phenolic and flavonoid contents. Malvidin 3-O-glucoside increased more than twofold, and delphinidin 3-O-glucoside and cyanidin 3-O-glucoside increased one fold according to the enzymatic method in the microwave treatments. The microwave technique was the most effective technique for antioxidant capacity, but sonication, cold and thermosonication results were lower than enzymatic treatment. The microwave and microwave and sonication enhanced the polyphenols with strong antioxidant power, such as catechin from 0.87 to 37.40 and trans-resveratrol from 0.09 to 0.23 mg/100 g, by comparison with the enzymatic technique. The findings suggested these two techniques were the most effective techniques for maceration.

Possibility to use the different sizes and colors of carrots for the production of juices - comparison of bioactive compounds, nutritional quality, pro-health properties, and sensory evaluation.

Carrot is a popular vegetable consumed by people of all age groups and is used in various food products because of its high nutritional content, especially vitamin A. In the present study, colorful fresh carrot juices of 12 carrot varieties were investigated for in vitro antidiabetic, anti-aging, and anti-obesity activities with antioxidant potential by ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric reducing ability of plasma) assays. The studied juices were also compared for physicochemical characteristics: titratable acidity, pH, pectin content, total soluble solids, dry mass, ash, viscosity, turbidity, osmolality, and color. The results of the study showed that normal purple carrot juice exhibited the best activities in all biological and antioxidant tests, except for anti-α-glucosidase activity. Normal purple carrot juice also had the highest total mineral content with elevated results for titratable acidity, pH, total soluble solids, dry mass, ash, viscosity, and osmolality. Purple carrot juices demonstrated elevated health-promoting activities and could be used in blended beverage recipes to attract children's attention. The results of sensorial characteristics (appearance, color, and taste) of juices, however, showed that people are more familiar with orange carrot products. © 2022 Society of Chemical Industry.

Sonication and thermal treatment of pineapple juice: Comparative assessment of the physicochemical properties, antioxidant activities and microbial inactivation.

Sonication is a novel processing method that is perceived as a substitute for heat treatment to ensure the safety of juice while reducing the loss of nutrients during processing. The impacts of sonication and thermal processing on pineapple juice, including physicochemical properties, antioxidant activities, and microbial inactivation, were studied. Pineapple juice was sonicated at 100 W and 140 W (for 5, 10 and 15 min) and heat-treated at 70 °C, 80 °C and 90 °C (for 1 min). Results indicated minor alterations in physicochemical characteristics after sonication and thermal processing, while sonication treatment showed better retention of the color, clarity, and browning index of pineapple juice. Moreover, sonication significantly increased the bioactive compounds (total carotenoids, ascorbic acid, phenolics, and flavonoids) and boosted the antioxidant activity of pineapple juice compared to the heat-processed juice. Although sonication reduced the microbial load significantly with the increasing exposure time, it could not thoroughly inactivate microorganisms like thermal treatment. Therefore, optimal sonication processing conditions are required to be determined. This study will help the food processors utilize sonication treatment in pineapple juice processing plants to inactivate microorganisms thoroughly while also boosting the nutritional value.

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Cladosporium parasphaerospermum, Cladosporium chlamydosporigenum, and Cladosporium compactisporum have all been discovered and characterized as new Cladosporium species. The three new species seemed to generate cold-active pectinases with high activity at pH 6.0 and 10 °C, pH 6.0 and 15 °C, and pH 5.0 and 15 °C, respectively, with the most active being C. parasphaerospermum pectinase. In submerged fermentation (SmF), C. parasphaerospermum produced the most cold-active pectinase with the highest activity and specific activity (28.84 U/mL and 3797 U/mg) after 8 days. C. parasphaerospermum cold-active pectinase was isolated using DEAE-Cellulose anion exchange resin and a Sephadex G 100 gel filtration column. The enzyme was purified 214.4-fold and 406.4-fold greater than the fermentation medium using DEAE-cellulose and Sephadex G 100, respectively. At pH 7.0 and 10 °C, pure pectinase had the highest activity (6684 U/mg), with Km and Vmax determined to be 26.625 mg/mL and 312.5 U/min, respectively. At 5 mM/mL, EDTA, MgCl2, and SDS inhibited the activity of pure pectinase by 99.21, 96.03, and 94.45%, respectively. The addition of 10 U/mL pure pectinase enhanced the yield of apple, orange, apricot, and peach juice by 17, 20, 13, and 24%, respectively, and improved the clarity and colour of orange juice by 194 and 339%, respectively. We can now add cold-active pectinase production to the long list of Cladosporium species that have been identified. We also report three new species that can be used in biotechnological solutions as active microbial pectinase producers. Although further research is needed, these distinct species might be used to decompose difficult and resistant pectinacious wastes as well as clear fruit juices.

Effect of β-glucosidase on the aroma of liquid-fermented black tea juice as an ingredient for tea-based beverages

The effects of β-glucosidase on the volatile profiles and aroma stability of black tea juice were evaluated using gas-chromatography-mass spectrometry coupled with sensory analysis. During liquid fermentation of tea leaves, the addition of β-glucosidase increased the concentration of aldehydes, strengthening the undesirable “green grassy” odour. However, the “green grassy” odour was counteracted by adding green tea extract during fermentation. At the same time, “flowery” flavour notes were enhanced, improving the overall aroma quality and strengthening the characteristic “sweet” aroma of black tea. Increased addition of β-glucosidase released more free aroma alcohols from their glucosides. Two “fruity” and “floral” aroma components, benzyl alcohol and phenylethyl alcohol, were not significantly affected by heat treatment (95 °C water bath) and the overall aroma stability was not significantly affected by β-glucosidase treatment. β-Glucosidase treatment improved the aroma, colour and overall suitability of fermented black tea juice as an ingredient for tea-based beverages.

Quality Changes in Black Chokeberry Juice Treated by Thermal-Assisted High Hydrostatic Pressure during Cold Storage.

The effects of thermal-assisted high hydrostatic pressure (TAHHP), high hydrostatic pressure (HHP), and thermal pasteurization (TP) treatments on the quality of aronia juice were evaluated in this study. The results showed that TAHHP and HHP significantly decreased the aerobic plate counts of aronia juice. No significant differences in terms of physicochemical properties, such as pH and total soluble solids, were observed between aronia juice treated with high pressure or thermal pasteurization treatment after 28 days of storage. TAHHP and HHP affected the colour and antioxidant characteristics of aronia juice, though to a significantly lower extent than TP. This result demonstrates that TAHHP and HHP can better maintain the original quality of aronia juice than TP. In summary, both TAHHP and HHP can maintain the microbiological safety and original quality characteristics of aronia juice. TAHHP can effectively increase the safety and duration of cold storage of aronia juice, and hence is highly useful for the juice industry.

Pengaruh Penambahan Pektin Kulit Pisang Kepok (Musa paradisiaca l.) Terhadap Karakteristik Fisiko-Kimia Sari Buah Naga Merah (hylocereus polyrhizus.)

The research aims to determine the effect of pectin from kepok banana peel as a stabilizer in red dragon fruit juice. The research applies Completely Randomized Design (CRD) with one factor, namely concentration of addition of pectin from kepok banana peel. The research factor comprises 4 levels, namely the concentration of addition of pectin from kepok banana peel (S0-banana peel pectin for 0 g. S1-banana peel pectin for 0.5 g. S2-banana peel pectin for 1 g, and S3-banana peel pectin for 1.5 g) and each treatment is repeated three times. Observation parameters consist of total dissolved solid, viscosity, pH, stability, and organoleptic. As a result, a significant effect is observable in the parameters of total dissolved solid, viscosity. and pH. On average, the panelists prefer the color of fruit juice with the addition of 1.5 g of banana peel pectin, which is 5,77 (like). In the meantime, an increase in the concentration of the addition of kepok banana peel pectin also increases the parameter values for total soluble solid ranging from 21.40 Brix, viscosity for 3550cp, and pH for 7.27%.

Study of polygalacturonase production by an Antarctic yeast and obtention of dragon fruit juice by maceration at mild temperature

The objectives of the present survey were to study the production and the biochemical characterization of an enzymatic cold-active extract with polygalacturonase activity, to perform a first insight into its possible application in fruit-juice obtention process, and to use this juice for food products preparation. The enzymatic extract was produced by the Antarctic yeast Mrakia sp. LP January 7, 2016 in a bioreactor with citric pectin as substrate. The polygalacturonase activity showed optimum conditions for reaction at 45 °C and pH 5.0. This activity was stable until 40 °C but deactivated at higher temperatures. Inhibition by Hg+2 suggested the presence of a thiol-dependent enzyme. A high-molecular polygalacturonase was detected in an SDS-PAGE and zymography analysis. Reaction-products were observed by thin-layer chromatography and suggested the endo-mode of action. The enzymatic extract had some accessory enzymes, such as pectin methylesterase, β-glucosidase, cellulase and inulinase. As a first insight into the potential use of this enzymatic extract for fruit maceration at mild temperatures, red and white dragon fruits were selected as models. At 23 °C, juice yields of 15%–23% higher than control were obtained after treatments, with a decrease of remanent solid of 25%–35%. The color of red dragon fruit juice increased with enzymatic treatment. Alginate balls that were prepared with the juices demonstrated a hardness of ∼10 N. The compression force of low-calorie sugar gels was 3.8 N. This work provided a complete study of the production, characterization, application and food product production using a new cold-active polygalacturonase extract of Antarctic origin.

Effect of ultrasound, centrifugation, and enzymatic pretreatments on phytochemical properties and membrane fouling during microfiltration of Sohiong (Prunus nepalensis L.) juice.

The study aimed to investigate the effects of various pretreatment methods on sohiong fruit juice during the microfiltration process. The ultrasound, centrifugation, and enzymatic pretreatment processes were used before the microfiltration process of the juice. The highest total phenolic content, anthocyanin content, and antioxidant activity of sohiong fruit juice were observed after enzymatic pretreatment. However, the browning index and color of the sohiong juice deteriorated with enzyme pretreatment. The fouling behavior of the microfiltration process for the pretreated sohiong juice was well explained by the complete pore-blocking model as the coefficient of determination (R 2 ) varied from 0.821 to 0.971. All pretreatment methods significantly (p ≤ 0.05) increased the initial flux of the microfiltration process, and the highest flux was observed for the enzyme pretreated juice. Furthermore, as compared to alternative pretreatments following microfiltration, the enzyme pretreated fruit juice had a high sensory acceptance. The work manifested a way to develop high-quality sohiong fruit juice with a low fouling tendency.

Deciphering the antibacterial activity and mechanism of p-coumaric acid against Alicyclobacillus acidoterrestris and its application in apple juice

Alicyclobacillus acidoterrestris has been regarded as the main hazardous factor causing the spoilage of commercially pasteurized fruit juice due to its unique thermo-acidophilic properties. p-Coumaric acid, primarily isolated from plants and having high biological activity, is supposed to be a viable food additive. Herein, the antibacterial activity and mechanism of p-coumaric acid against A. acidoterrestris and its application in apple juice were investigated. The results showed that p-coumaric acid had an active inhibition against A. acidoterrestris vegetative cells, and both minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were 0.2 mg/mL. MIC of p-coumaric acid against spores was also 0.2 mg/mL, while MBC was >1.6 mg/mL. Treatment of p-coumaric acid caused significant damage of cell integrity, decrease of intracellular ATP, hyperpolarization of cell membrane, degradation of whole cell protein, and malformation of cell morphology. Agarose gel retardation and fluorescence spectroscopy assays revealed that p-coumaric acid could interact with DNA and intercalate into the groove of DNA double helix to disturb normal cellular functions. Furthermore, the addition of p-coumaric acid at MIC caused 2.07 log drop in A. acidoterrestris vegetative cells and effectively inhibited the germination of spores in apple juice storage at 20 °C for 7 days. The low-temperature storage (4 °C) could inactivate A. acidoterrestris cells in apple juice, but the addition of p-coumaric acid further accelerated degradation of vegetative cells and the inactivation time was shortened from 5 days to 3 days compared with control. Importantly, the addition of p-coumaric acid could increase total phenolic content and ABTS radical scavenging activity and had no remarkable influence on pH, total soluble solids, color and sensory qualities of apple juice, regardless of storage temperature. These results revealed that p-coumaric acid could be expected to serve as an alternative or supplement agent for inhibiting the development of A. acidoterrestris in fruit juice.

Effect of ascorbic acid and acerola juice on some quality properties of aseptic filled apple juice

Abstract Quality and storability are crucial factors in production of apple juice. The main goal of this study was investigation of the effects of ascorbic acid and acerola juice on the changes of some sensorial parameters and bioactive compounds of aseptically filled and industrial scale produced apple juice during storage for 12 months. While the viscosity and pH of apple juice did not change significantly, the ΔE* peaked (20–30) at month 6 of the storage period. The colour of apple juice was lighter than at the beginning of storage. Maximum total phenolic contents were 1,100, 1,400, and 1,250 mg L−1 in the control, ascorbic acid supplemented, and acerola added samples, respectively. Other parameters (antioxidant capacity, ascorbic acid, browning index, etc.) peaked in month 4. Acerola was a good alternative anti-browning and antioxidant agent for the treatment of apple juice in the processing. The antioxidant capacity of apple juice treated with acerola was higher than with ascorbic acid. The results were obtained with industrial samples, thus, they can serve as a very good base for the optimisation process and industrial production without the need for scale-up.