Journal Of Allergy Research Articles

Occupational Asthma

MOC as well as CME credit can now be obtained, free for a limited time, by reviewing this Difficult Cases presentation. Please note the following instructions. 1.Review the target audience, learning objectives, and author disclosures. 2.Complete the pre-test online at the JACI: In Practice homepage at www.jaci-inpractice.org (click on the Online CME heading). 3.Follow the online instructions to review the full online version of the presentation. 4.Complete the post-test. At this time, you will have earned 1.0 AMA PRA Category 1 CME Credit™. 5.Approximately 4 weeks later you will receive an online assessment regarding your application of this article to your practice. Once you have completed this assessment, you will be eligible to receive 2 MOC Part II Self-Assessment credits from the American Board of Allergy and Immunology. CME Exam: Occupational AsthmaThe Journal of Allergy and Clinical Immunology: In PracticeVol. 4Issue 4Preview Full-Text PDF

Anaphyl Actic Reaction After the Intravenous Administration of Omeprazole: A Case Report

SESSION TITLE: Critical Care SESSION TYPE: Case Report Poster PRESENTED ON: Sunday, April 17, 2016 at 11:45 AM - 12:45 PM INTRODUCTION: Omeprazole, one of proton pump inhibitors, is widely used and a rare cause of anaphylactic reactions such as dyspnea and shock, which could be extremely life threatening. This is an observational descriptive case report. CASE PRESENTATION: A 64-year-old non-atopic man with lung cancer was admitted in our unit for chemotherapy. He had two similar allergic episodes with good outcomes after active treatment in the past month. For the first time, allergic reaction, including urticaria, dyspnea, appeared within a few minutes after the intravenous administration of 800mg pemetrexed disodium for injection followed by 40mg omeprazole. In the second preparation we adjusted the medication order. Omeprazole (40mg) was infused firstly, unfortunately, severe allergic event occurred within 10 minutes. The patient experienced urticaria, wheeze, decline of oxygen saturation (less than 90%), low systolic blood pressure (less than 90 mmHg), and tachycardia (more than 130 beats per min). Serum IgE measurement was significantly higher than that in normal subjects within 24 h after the second event and intradermal skin test with omepazole was positive (the patients had signed written informed consent before treatment and check). He received immediate treatment with oxygen inhalation followed by intravenous dexamethasone and promethazine, and the symptoms disappeared in less than 2 hours. Under strict medical supervision, no adverse reaction was found induced by lansoprazole for replacement therapy after skin test came back negative. DISCUSSION: It was reported that omeprazole would lead to allergic reactions. In this case, intradermal skin test with omepazole was positive. Several of these reports described anaphylactic symptoms occurring within few minutes of ingestion of omeprazole, which was suggestive of an IgE-mediated event. In our patient, the level of serum IgE was significantly higher than that in normal subjects after exposure to omeprazole. Although more studies would be needed to confirm this result, it is highly suggestive that omeprazole is capable of triggering a specific IgE mediated response. Some reports showed lansoprazole caused anaphylactic reaction during oral or intravenous infusion. It had been proved that skin test had high sensitivity and specificity. In this case, skin test with lansoprazole was negative, therefore we used lansoprazole instead of omeprazole to combate the potential GI side effects of chemotherapy. The patient showed good tolerance. CONCLUSIONS: It is highly suggestive that omeprazole is capable of triggering anaphylactic reaction mediated by IgE. Reference #1: Ottervanger JP, Phaff RA, Vermeulen EG, Stricker BH: Anaphylaxis to omeprazole. The Journal of allergy and clinical immunology 1996, 97(6):1413-1414. [PMID: 8648040] DISCLOSURE: The following authors have nothing to disclose: Kun Xiao, Yi Hu, Lixin Xie No Product/Research Disclosure Information

Editorial introductions

Editorial introductions

Editorial introductions

Editorial introductions

Retraction Note: Meeting Abstract: A post-hoc qualitative analysis of real time heads-up pollen counting versus traditional microscopy counting in the environmental exposure unit (EEU)

Retraction This meeting abstract [1], published in the Canadian Society of Allergy and Clinical Immunology and AllerGen Abstracts 2014 supplement, has been retracted from Allergy, Asthma & Clinical Immunology by the authors. The meeting abstract has previously been published in Journal of Allergy and Clinical Immunology [2] and was inadvertently submitted to Allergy, Asthma & Clinical Immunology as part of the supplement. The authors apologise for any inconvenience that this may have caused.

Editorial introductions

Current Opinion in Allergy and Clinical Immunology was launched in 2001. It is one of a successful series of review journals whose unique format is designed to provide a systematic and critical assessment of the literature as presented in the many primary journals. The fields of allergy and clinical immunology are divided into 14 sections that are reviewed once a year. Each section is assigned a Section Editor, a leading authority in the area, who identifies the most important topics at that time. Here we are pleased to introduce the Journal's Section Editors for this issue. SECTION EDITORS Susan M. TarloSusan M. TarloDr Susan M. Tarlo, MBBS, FRCP(C) is a Professor in the Department of Medicine and in the Dalla Lana School of Public Health at the University of Toronto, Canada. She is a respiratory physician at the University Health Network, Toronto Western Hospital, Canada and at the Gage Occupational and Environmental Health Unit and Centre of Research Excellence in Occupational Diseases of St Michael's Hospital in Toronto, Canada. Her main areas of clinical practice and research are in occupational and environmental lung diseases and allergic responses, especially occupational asthma. She initially graduated from medical school at London University, England, and underwent postgraduate training in England for four years before coming to Queen's University, Kingston, Ontario, Canada for a year as a fellow in allergy and clinical immunology, followed by a respiratory medicine residency for two years at McMaster University, Hamilton, Canada. She has been in Toronto as a staff physician at the Toronto General Hospital and then at Toronto Western Hospital as well as at the Gage Occupational and Environmental Health Unit since 1976 as well as holding academic positions at the University of Toronto since that time. Piero MaestrelliPiero MaestrelliDr Maestrelli, MD, completed his medical degree at University of Padova, Italy, and then specialised in occupational medicine and in pulmonology. He completed a two year post doctoral fellowship at the Institute of Occupational Medicine, University of Padova, and then a two year research fellowship at the Department of Allergy and Clinical Immunology of Cardiothoracic Institute, London, UK. Dr Maestrelli has written over 150 scientific papers and book chapters, and has lectured nationally and internationally. He has been Chairman of the Interest Group on Occupational Allergy of European Academy of Allergology and Clinical Immunology for three years. He is currently Professor of Occupational Medicine at University of Padova, Department of Cardiologic, Thoracic and Vascular Sciences, Italy, and Head of the Laboratory of Lung Pathophysiology of the Unit of Occupational Medicine at the University Hospital of Padova. Leonard B. BacharierLeonard B. BacharierDr Bacharier is Professor of Pediatrics and Medicine at Washington University School of Medicine, USA and Clinical Director of the Division of Pediatric Allergy, Immunology and Pulmonary Medicine, and Unit co-Leader for the Pediatric Patient Orientated Research Unit. He received his undergraduate degree in biophysics from The Johns Hopkins University, USA and his medical degree from Washington University School of Medicine. Dr Bacharier was an intern and resident in pediatrics at St. Louis Children's Hospital and a fellow in pediatric allergy and immunology at Children's Hospital, Boston and Harvard Medical School, USA. He was a recipient of the 2005 Samuel R. Goldstein Leadership Award in Medical Student Education from Washington University School of Medicine and is a member of several organizations including the American Academy of Allergy, Asthma and Immunology and the American Thoracic Society. Dr Bacharier's research focuses on childhood asthma. Specifically, he is a principal investigator in the NHLBI-funded AsthmaNet, a multi-centered network examining therapeutic approaches to childhood asthma. He is an Investigator in the NHLBI's Severe Asthma Research Program (SARP) and the Inner City Asthma Consortium's URECA study examining the influence of urban environments on the development of asthma and allergic disorders. He is also an investigator in the American Lung Association's Asthma Clinical Research Center Network. Dr Bacharier is also currently investigating the role of early infection with Respiratory Syncytial Virus upon the subsequent development of asthma. These research projects have been published in numerous journals, including the New England Journal of Medicine, The Journal of Allergy and Clinical Immunology, Pediatrics, and The American Journal of Respiratory and Critical Care Medicine. Dr Bacharier serves on the Editorial Boards of The Journal of Allergy and Clinical Immunology and The Journal of Allergy and Clinical Immunology: In Practice, and has been a guest editor for Immunology and Allergy Clinics of North America.

Hypereosinophilic Disorders

Credit can now be obtained, free for a limited time, by reading the review articles in this issue. Please note the following instructions. CME Exam: Difficult Cases: Hypereosinophilic DisordersThe Journal of Allergy and Clinical Immunology: In PracticeVol. 3Issue 2Preview Full-Text PDF

ATA Practice Guidelines for Live, On-Demand Primary and Urgent Care

ATA Practice Guidelines for Live, On-Demand Primary and Urgent Care

Strategy support for the Post‐Market Monitoring (PMM) of GM plants: Review of existing PMM strategies developed for the safety assessment of human and animal health

Strategy support for the Post‐Market Monitoring (PMM) of GM plants: Review of existing PMM strategies developed for the safety assessment of human and animal health

10th anniversary of allergy, asthma & clinical immunology.

10th anniversary of allergy, asthma & clinical immunology.

Editorial introductions

Editorial introductions

Editorial introductions

Editorial introductions

The expression of cannabinoid receptor 1 is significantly increased in atopic patients

The expression of cannabinoid receptor 1 is significantly increased in atopic patients

Hereditary Angioedema with Normal C1-INH (HAE Type III)

Hereditary angioedema (HAE) with normal C1 inhibitor (C1-INH), also known as HAE type III, is a familial condition only clinically recognized within the past three decades. Similar to HAE from C1-INH deficiency (HAE types I and II), affected individuals experience unpredictable angioedema episodes of the skin, gastrointestinal tract, and airway. Unique clinical features of HAE with normal C1-INH include the predominance of affected women, frequent exacerbation by estrogen, and a prominence of angioedema that involves the face and oropharynx. The underlying pathophysiology of HAE with normal C1-INH is poorly understood, but indirect evidence points to contact pathway dysregulation with bradykinin-mediated angioedema. Currently, evaluation is complicated by a lack of confirmatory laboratory testing such that clinical criteria must often be used to make the diagnosis of HAE with normal C1-INH. Factor XII mutations have been identified in only a minority of persons affected by HAE with normal C1-INH, limiting the utility of such analysis. To date, no controlled clinical studies have examined the efficacy of therapeutic agents for HAE with normal C1-INH, although published evidence supports frequent clinical benefit with medications shown effective in HAE due to C1-INH deficiency.

Crowdsourcing to explore views about asthma severity among asthma experts

Defining severe asthma and degrees of severity of asthma has been complicated, with classifications changing over time and also in different publications. The aim of the current investigation was therefore to start explore views about asthma severity among a wide group of asthma experts. We defined an asthma expert as an individual that since January 2005 has been an author for correspondence on a scientific publication on asthma (search term hit) in any of the top seven respiratory or allergy journals (impact factor). A brief questionnaire was distributed to 4791 e-mail addresses manually curated from the journals. Nine days after the first e-mail was circulated, and four days after a reminder e-mail, 725 individuals had responded. Of the respondents, 84.5% stated that they are either “very knowledgeable” or “knowledgeable” in asthma, and 14% state that they understand some aspects of asthma. A large majority (80.8%) of respondents suggested that reaching out to a large number of experts to crowdsource on topics related to asthma is “reasonable”, whereas 8.9% did not think so. Respondents were also asked which crucial factors contribute to asthma severity, and the responses were: 1) exacerbation frequency (84%), 2) exacerbation severity (78.4%, 3) need of extensive medication (72.2%), 4) low lung function (57.7%), 5) night-time awakenings (50.1%), 6) degree of drop in lung function when attacks occur (44.5%), 7) number of asthma symptoms (40.3%), 8) presence of any comorbidity (39.6%), 9) degree of bronchial hyperresponsiveness (39.2%), 10) lack of control according to brief questionnaire (36.8%), 11) degree of variability of lung function (36%). A small majority (57%) voted that the definition of “severe asthma” should be given to those that are uncontrolled with current conventional therapy, but an important minority (38.3%) disagreed with this definition. Outreach to a wide group of experts in the field of asthma resulted in a significant response rate, where a vast majority acknowledge themselves as experts in the field, and also thought that this approach is reasonable. Crowdsourcing may be efficient to develop wide consensus on different aspects of asthma, including a definition of severe asthma. However, additional crowdsourcing surveys, involving comments from current respondents, will be needed. This work was financed by EAACI.

Editorial introductions

Editorial introductions

Construction of a naive human phage scFv library and selection, identification of human scFvs

Event Abstract Back to Event Construction of a naive human phage scFv library and selection, identification of human scFvs Qing Yuan1, Siji Nian1*, Xiyuan Guo1, Yan Gao1 and Jiajia Liu1 1 Luzhou medcal college, department of Inmmunology, China The IL-4, IL-13 and IL-33 are key components in the development of allergic disease such as asthma. To construction of human non-immune library and selection of human scFvs against the three cytokines, the mRNA of human peripheral blood cells (PBMC) was extracted from healthy volunteer, and then the cDNA library was constructed. The variable region of heavy chain (VH) and light chain (VL, including Vκ and Vλ) were amplified from full length cDNA library. The VH and VL were ligated with linker and the DNA library of single-chain Fv fragments (scFvs) was developed. After transforming into E.coli TG1, the phage library was constructed. The antigen IL-4, IL-13 and IL-33 were cloned, expressed and identified. The scFv library was enriched for three rounds by phage display for selection of human scFvs against IL-4, IL-13 and IL-33. By PCR, forty two bands of VH, sixteen bands of Vκ and eighteen bands of Vλ were got. And the scFv library was constructed successfully with 2.5x108 development clones with full length scFv inserts. Through three rounds of phage display, 30% clones showed positive using ELISA for detection. Four to five scFvs with high affinity against IL-4, IL-13 and IL-33 respectively were screened, expressed, and identified by Biacore, western blot and sequencing. The results showed the size of proteins of identified scFvs were about 32 kDa and the sequences were correct. The human non-immune library was constructed and the scFvs specific to IL-4, IL-13 and IL-33 respectively were selected successfully. Acknowledgements This work was supported by the Natural Science Foundation of China (No. 31000415) and the project of Science and Technology Department of Sichuan Province (No.2012JQ0018). References ［1］. Moulin, D., Donze, O., Talabot-Ayer, D., Mezin, F., Palmer, G., & Gabay, C. (2007). Cytokine, 40, 216–225. ［2］. Komai-Koma, M., Xu, D., Li, Y., McKenzie, A. N., McInnes, I. B., & Liew, F. Y. (2007). European Journal of Immunology, 37, 2779–2786. ［3］. Suzukawa, M., Iikura, M., Koketsu, R., Nagase, H., Tamura, C., Komiya, A., et al. (2008). Journal of Immunology, 181, 5981–5989. ［4］. Cherry, W. B., Yoon, J., Bartemes, K. R., Iijima, K., & Kita, H. (2008). The Journal of Allergy and Clinical Immunology, 121, 1484–1490. ［5］. Suzukawa, M., Koketsu, R., Iikura, M., Nakae, S., Matsumoto, K., Nagase, H., et al. (2008). Laboratory Investigation, 88, 1245–1253. ［6］. Kondo, Y., Yoshimoto, T., Yasuda, K., Futatsugi-Yumikura, S., Morimoto, M., Hayashi, N., et al. (2008). International Immunology, 20, 791–800. ［7］. Qing, Y., Zhongkang,W., Siji, N., Youping, Y., Gang, C., & Yuxian, X. (2009). Applied Biochemistry and Biotechnology, 152, 224–234. ［8］. Kurowska-Stolarska, M., Kewin, P., Murphy, G., Russo, R. C., Stolarski, B., Garcia, C. C., et al. (2008). Journal of Immunology, 181, 4780–4790. Keywords: Human scFvs, phage display, IL-4, IL-13, IL-33 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Yuan Q, Nian S, Guo X, Gao Y and Liu J (2013). Construction of a naive human phage scFv library and selection, identification of human scFvs. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00521 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Siji Nian, Luzhou medcal college, department of Inmmunology, Luzhou, Sichuan province, 646000, China, sijinian@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Qing Yuan Siji Nian Xiyuan Guo Yan Gao Jiajia Liu Google Qing Yuan Siji Nian Xiyuan Guo Yan Gao Jiajia Liu Google Scholar Qing Yuan Siji Nian Xiyuan Guo Yan Gao Jiajia Liu PubMed Qing Yuan Siji Nian Xiyuan Guo Yan Gao Jiajia Liu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Peptide Microarrays with Near Zero background enable high quality and massive data production from seroscreening of autoimmune diseases

Peptide Microarrays with Near Zero background enable high quality and massive data production from seroscreening of autoimmune diseases

News in the pathophysiology of asthma

nema

Brazilian Journal of Allergy and Immunology: on its way to internationalization

Brazilian Journal of Allergy and Immunology: on its way to internationalization