Diospyros Kaki Research Articles

Secondary metabolites and transcriptomic analysis of novel pulcherrimin producer Metschnikowia persimmonesis KIOM G15050: A potent and safe food biocontrol agent

Metschnikowia persimmonesis, a novel endophytic yeast strain isolated from Diospyros kaki calyx, possesses strong antimicrobial activity. We investigated its potential use as an environmentally safe food biocontrol agent through genomics, transcriptomics, and metabolomics. Secondary metabolites were isolated from M. persimmonesis, followed by chemical structure elucidation, PUL gene cluster identification, and RNA sequencing. Pulcherrimin was isolated using 2 M NaOH, its structure was confirmed, and the yield was quantified. Biocontrol efficacy of M. persimmonesis on persimmon fruits and calyx was evaluated by assessing lesion diameter and disease incidence. Following compounds were isolated from M. persimmonesis co-culture with Botrytis cinerea and Fusarium oxysporum: fusaric acid, benzoic acid, benzeneacetic acid, 4-hydroxybenzeneacetic acid, 4-(-2-hydoxyethyl)-benzoic acid, cyclo (Leu-Leu), benzenemethanol, 4-hydroxy-benzaldehide, 2-hydroxy-4-methoxy-benzoic acid, 4-hydroxy-benzoic acid, lumichrome, heptadecanoic acid, and nonadecanoic acid. Exposing M. persimmonesis to different growth media conditions (with or without sugar) resulted in the isolation of five compounds: Tyrosol, Cyclo (Pro-Val), cyclo(L-Pro-L-Tyr), cyclo(Leu-Leu), and cyclo(l-tyrosilylicine). Differentially expressed gene analysis revealed 3264 genes that were significantly expressed (fold change ≥2 and p-value ≤0.05) during M. persimmonesis growth in different media, of which only 270 (8.27%) showed altered expression in all sample combinations with Luria–Bertani Agar as control. Minimal media with ferric ions and tween-80 triggered the most gene expression changes, with the highest levels of PUL gene expression and pulcherrimin yield (262.166 mg/L) among all media treatments. M. persimmonesis also produced a higher amount of pulcherrimin (209.733 mg/L) than Metschnikowia pulcherrima (152.8 mg/L). M. persimmonesis inhibited the growth of Fusarium oxysporum in persimmon fruit and calyx. Toxicity evaluation of M. persimmonesis extracts showed no harmful effects on the liver and mitochondria of zebrafish, and no potential risk of cardiotoxicity in hERG-HEK293 cell lines. Thus, M. persimmonesis can be commercialized as a potent and safe biocontrol agent for preserving food products.

Quantitative genotyping of CPCNA locus advances genetic strategies in PCNA persimmon breeding

Persimmon (Diospyros kaki Thunb., 2n = 6x = 90) is a major fruit crop in eastern Asia, known for its unique in accumulating large amounts of proanthocyanidins (PAs) in the fruit flesh, resulting in strong astringency. The Pollination constant and non-astringent (PCNA) mutation, characterized by reduced PAs, allows for consumption before softening, akin to an apple. Two distinct types of PCNA persimmons, Japanese PCNA (J-PCNA) and Chinese PCNA (C-PCNA), exhibit different genetic regulatory mechanisms. The J-PCNA type is conferred by homozygous recessive alleles (ast), while C-PCNA possesses at least one dominant allele (CPCNA) at the CPCNA locus. The probability of generating PCNA individuals depends on the presence of ast or CPCNA alleles in parental plants. Previous research has quantified dosages of AST alleles in major Japanese cultivars, the status of AST alleles, especially CPCNA alleles, in Chinese cultivars remains undisclosed. This lack of understanding creates a significant obstacle in PCNA persimmon breeding efforts, particularly for the C-PCNA type. In this study, we developed a method to quantify the CPCNA locus by detecting the allele dosage of RO2, a marker strongly associated with CPCNA alleles across cultivars, using quantitative real-time PCR (qPCR). Our findings reveal that most C-PCNA cultivars and androecious germplasm exhibiting the PCNA trait contain only one CPCNA allele (Bbbbbb). Furthermore, we observed that the average ast allele dosage in Chinese genotypes was lower than that in Japanese non-PCNA genotypes. Quantifying the CPCNA locus assists in selecting PCNA candidate and also cultivating parents with sufficient CPCNA alleles (more than quadruple dose), ensuring that the offspring crossed with non-PCNA types inherit the PCNA trait. This approach will undoubtedly accelerate the breeding of novel PCNA cultivars and could also prevent inbreeding depression in PCNA breeding practices when crosses between J-PCNA cultivars. Our method provides crucial insights for enhancing PCNA persimmon breeding programs and advancing the development of improved cultivars.

UHPLC-MS/MS analysis and protective effects on neurodegenerative diseases of phenolic compounds in different parts of Diospyros kaki L. cv. Mopan

Persimmon (Diospyros kaki L. cv. Mopan.), an important commercial crop belonging to the genus of Diospyros in the Ebenaceae family, is rich in bioactive phenolic compounds. In this study, the phenolic compounds from fruits, leaves, and calyces of persimmon were qualitatively and quantitatively determined by UPLC-Q-Exactive-Orbitrap/MS and UPLC-QqQ-MS/MS, respectively. Furthermore, the role of phenolic extract from different parts of persimmon on neuroprotective activity in vitro, through against oxidative stress and anti-neuroinflammation effect was firstly evaluated. The results showed that 75 phenolic compounds, and 3 other kinds of compounds were identified, among which 44 of phenolic compounds were quantified from different parts of persimmon. It is the first time that epicatechin-epigallocatechin, catechin-epigallocatechin, catechin-epigallocatechin (A-type), and glycoside derivatives of laricitrin were identified in persimmon extract. The dominated phenolic compounds in three parts of persimmon were significantly different. All phenolic extracts from each part of persimmon showed strong neuroprotective activities against H2O2-induced oxidative stress in PC-12 cells and LPS-induced BV2 cells. The fruit extract presented the strongest activity, followed by calyx and leaf extract. The systematic knowledge on the phytochemical composition along with activity evaluation of different parts of persimmon could contribute to their targeted selection and development.

Investigation of Chemical Composition, Antioxidant and Alpha-Amylase Inhibitory Properties of Pulp and Peel of Tunisian Diospyros kaki L. Fruit

Investigation of Chemical Composition, Antioxidant and Alpha-Amylase Inhibitory Properties of Pulp and Peel of Tunisian Diospyros kaki L. Fruit

Pulp or Peel? Comparative Analysis of the Phytochemical Content and Selected Cosmetic-Related Properties of Annona cherimola L., Diospyros kaki Thumb., Cydonia oblonga Mill. and Fortunella margarita Swingle Pulp and Peel Extracts.

Fruit peels might be a valuable source of active ingredients for cosmetics, leading to more sustainable usage of plant by-products. The aim of the study was to evaluate the phytochemical content and selected biological properties of hydroglycolic extracts from peels and pulps of Annona cherimola, Diospyros kaki, Cydonia oblonga, and Fortunella margarita as potential cosmetic ingredients. Peel and pulp extracts were compared for their antiradical activity (using DPPH and ABTS radical scavenging assays), skin-lightening potential (tyrosinase inhibitory assay), sun protection factor (SPF), and cytotoxicity toward human fibroblast, keratinocyte, and melanoma cell lines. The total content of polyphenols and/or flavonoids was significantly higher in peel than in pulp extracts, and the composition of particular active compounds was also markedly different. The HPLC-MS fingerprinting revealed the presence of catechin, epicatechin and rutoside in the peel of D. kaki, whereas kaempferol glucoside and procyanidin A were present only in the pulp. In A. cherimola, catechin, epicatechin and rutoside were identified only in the peel of the fruit, whereas procyanidins were traced only in the pulp extracts. Quercetin and luteolinidin were found to be characteristic compounds of F. margarita peel extract. Naringenin and hesperidin were found only in the pulp of F. margarita. The most significant compositional variety between the peel and pulp extracts was observed for C. oblonga: Peel extracts contained a higher number of active components (e.g., vicenin-2, kaempferol rutinoside, or kaempferol galactoside) than pulp extract. The radical scavenging potential of peel extracts was higher than of the pulp extracts. D. kaki and F. margarita peel and pulp extracts inhibited mushroom and murine tyrosinases at comparable levels. The C. oblonga pulp extract was a more potent mushroom tyrosinase inhibitor than the peel extract. Peel extract of A. cherimola inhibited mushroom tyrosinase but activated the murine enzyme. F. margarita pulp and peel extracts showed the highest in vitro SPF. A. cherimola, D. kaki, and F. margarita extracts were not cytotoxic for fibroblasts and keratinocytes up to a concentration of 2% (v/v) and the peel extracts were cytotoxic for A375 melanoma cells. To summarize, peel extracts from all analyzed fruit showed comparable or better cosmetic-related properties than pulp extracts and might be considered multifunctional active ingredients of skin lightening, anti-aging, and protective cosmetics.

Identification of phytoplasmas in stone fruit (Prunus sp.) and persimmon (Diospyros kaki L.) trees exhibiting leaf alterations and witches'‐broom in Jordan

AbstractDuring field surveys conducted in 2020 in Jordanian orchards, phytoplasma‐like symptoms (leaf yellowing/reddening and rolling, and witches'‐broom) were observed in three stone fruit species (peach, European plum, sweet cherry) and persimmon. Molecular analyses identified phytoplasma strains belonging to the species ‘Candidatus Phytoplasma solani’ (subgroup 16SrXII‐A) as largely prevalent in stone fruit and persimmon symptomatic plants. Moreover, ‘Ca. Phytoplasma omanense’ (16SrXXIX‐B) was found in few European plum symptomatic plants. In previous studies, such phytoplasma strains were identified in other important crops (almond, pomegranate, and grapevine) and in several putative insect vectors, suggesting their complex ecology in Jordan. Further studies are needed to in‐depth investigate the diffusion of phytoplasma‐associated diseases of stone fruits throughout the Country, to clarify their aetiology, and to study their epidemiological pattern(s).

Determination of the Boundary between Juvenile–Mature Wood of Diospyros kaki and Their Wood Anatomical Variations

Determination of the Boundary between Juvenile–Mature Wood of Diospyros kaki and Their Wood Anatomical Variations

À propos d’un cas pédiatrique d’allergie au kaki

Food allergy to persimmon (Diospyros kaki) is exceedingly rare. We report a case involving a 3.33-year-old child who experienced an episode of palpebral edema with pruritus and facial erythema following the first ingestion of this fruit. Allergological investigation reveals a biological sensitivity to persimmon, with specific immunoglobulin E levels at 0,79 kU/L, as well as a positive skin prick tests to persimmon fresh extract. An oral provocation test was also conducted, demonstrating no reaction to the ingestion of 1 then 2 grams of persimmon, but a similar reaction to the initial episode when ingesting 5 grams (resulting in a total cumulative dose of 8 grams). Our observation describes the pediatric manifestations of this allergy along with a literature review.

Physiological Characteristics and Transcriptional Differences of Growth Traits of Persimmon with Different Ploidy

Ploidy breeding is one of the important approaches for persimmon (Diospyros kaki Thunb.) genetic improvement, and vegetative growth of seedlings is the basis for subsequent fruit development. Therefore, the physiological characteristics and transcriptional differences of seedling growth traits in different ploidy persimmon germplasm were studied in this study, which provided a theoretical basis for fruit evaluation and breeding of new polyploid persimmon varieties. Nonuploid and its full-sib hexaploid seedlings obtained from endosperm culture were used as materials. By observing plant phenotype, leaf tissue section, endogenous hormone content, and transcriptome sequencing, the phenotype and physiological characteristics of different ploidy Persimmon seedlings were compared, as well as the differences in transcription levels. (1) Compared with hexaploid seedlings, the nonuploid were more robust and compact, and the leaves were obviously thicker. The cell size of leaf veins and parenchyma were significantly different between the different ploidy plants. (2) The contents of Salicylic Acid (SA), Jasmonic Acid (JA), gibberellin A1 (GA1), gibberellin A3 (GA3) and Indole-3-acetic acid (IAA) in nonuploid leaves were significantly higher than those in hexaploid leaves, while the contents of cytokinin trans-zeanoside (Tzt) and dihydrozeanoside (DZR), N6-isopentenyladenine (iP) and Jasmonoyl-L-isoleucine (JA-ILE) in nonuploid leaves were significantly lower than those in hexaploid leaves. (3) A total of 5796 differentially expressed genes were identified in nonuploid and hexaploid leaves. These differentially expressed genes were mainly related to photosynthesis, plant-pathogen interaction, etc. Among them, YUCCA genes, GA3ox genes, and IPT genes related to hormone synthesis were significantly differentially expressed in the nonuploid and hexaploid leaves. It is speculated that it may be the key regulatory gene that leads to the difference in IAA, gibberellin (GA), and indolepropionic acid (IPA) levels in the nonuploid and the hexaploid. The growth traits of the new Persimmon germplasms with different ploidy were significantly different. The nonuploid plants were shorter and more compact, and the leaves were larger and thicker. These traits were closely related to the content of endogenous hormones, and the balance of endogenous hormones was affected by gene expression. In addition, based on the biological processes involved in hormones and differentially expressed genes, it is speculated that the nonuploid may be superior to the hexaploid in terms of resistance.

Identification of main metabolites correlated with the sensory attributes of Diospyros kaki. cv. “Taishuu” through a large-scale comprehensive analysis by sensory evaluation, electronic tongue and metabolomics

Diospyros kaki. cv. “Taishuu” is currently the most popular sweet persimmon variety globally. However, the metabolic causes for the differences in taste are unknown due to the lack of a comprehensive investigation of the metabolites in sweet persimmon fruit. Therefore, we selected more than 2500 fruits of Taishuu sweet persimmon from 35 orchards across China, established sensory evaluation standards for the taste quality of Taishuu sweet persimmon, clarified that flavor was the main factor affecting its comprehensive taste, and further concluded that richness was the main factor affecting its flavor. Metabolomics technology was used to find the differential metabolites affecting the taste of Taishuu sweet persimmon, to find the differential metabolites of each index of the electronic tongue, and to identify 50 differential metabolites affecting the flavor, mainly amino acids. All metabolites were analyzed using WGCNA and 23 common metabolites were found. This study provides a basis for the quality improvement of Taishuu sweet persimmon.

Extraction, purification, and evaluation of bioactivities of total triterpenoids from Persimmon (Diospyros kaki L.f.) Leaves

The current research focused on enhancing the extraction and purification processes of triterpenoids found in persimmon leaves, as well as assessing their biological effects. The extraction yield of triterpenoids was 8.26%. Activated carbon was used to purify the extracts, resulting in an increase in the content of triterpenoids in the final product from 24.43% to 79.66%. The recovery yield of triterpenoids from the ethanol extract was 73.57%. Ethanol was recovered and reused as a solvent. The total triterpenoids exhibited cytotoxic activities against several cancer cell lines, with IC50 values of approximately 32.49–55.02 μg/mL, as well as anti-inflammatory activities (IC50 = 29.55 ± 1.67 μg/mL). The total extract and total triterpenoids demonstrated significant inhibition against α-glucosidase, having IC50 values of 0.18 ± 0.05 μg/mL and 2.12 ± 0.15 μg/mL, respectively. Additionally, for antioxidant activity, the IC50 values of the total extract and total triterpenoids were 12.53 ± 0.20 and 13.92 ± 0.18 μg/mL, respectively. The triterpenoids showed higher inhibition of acetylcholinesterase compared with the total extract (IC50: 40.07 ± 2.54 and 112.22 ± 9.68 μg/mL, respectively). However, they did not show inhibitory activity against bacteria or fungi. In conclusion, information about the extraction, purification and biological activities of total triterpenoids from persimmon leaves was fully provided for the first time.

Persimmon fruit downstream prospects: a promising solution to increase farmer income in Malang Regency, East Java, Indonesia

This study explores the potential and benefits of persimmon fruit (Diospyros kaki) for human health and the prospects for producing and downstream this fruit in Indonesia. Persimmons have rich nutritional content, including vitamin A, vitamin B6, vitamin C, and vitamin K, as well as essential minerals such as potassium. This content has been known to have various benefits, including its ability to lower blood cholesterol levels, maintain eye health, and even potentially as an antiviral agent. In Indonesia, persimmons are less popular, but some regions such as Malang (East Java) and Toba (North Sumatra) produce them to increase farmers’ income. This research also explores the downstream potential of persimmon fruit products. This step is part of the community’s economic empowerment strategy by utilizing the potential of environmentally sound natural resources in East Java, Indonesia. This study uses the SWOT analysis method to determine internal and external factors that can see the prospects of persimmon farmers and factors that affect the downstream development of persimmon fruit products. This study aims to identify the prospects of persimmon farmers and components that affect farmers’ income through sustainable downstream of persimmon fruit products.

Genetic diversity of apple fruit crinkle viroid populations in Japanese persimmons and the infectivity of a predominant sequence variant to tomato plants

Genetic diversity of apple fruit crinkle viroid populations in Japanese persimmons and the infectivity of a predominant sequence variant to tomato plants

Comparative transcriptome analysis of persimmon somatic mutants (Diospyros kaki) identifies regulatory networks for fruit maturation and size.

Bud sports in fruit crops often result in new cultivars with unique traits, such as distinct fruit size and color, compared to their parent plants. This study investigates the phenotypic differences and gene expression patterns in Tonewase and Ohtanenashi persimmon bud sports compared to those in their parent, Hiratanenashi, based on RNA-seq data. Tonewase is characterized by early maturation, whereas Ohtanenashi is noted for its larger fruit size. Despite the importance of these traits in determining fruit quality, their molecular bases in persimmons have been understudied. We compared transcriptome-level differences during fruit development between the bud sport samples and their original cultivar. Comprehensive transcriptome analyses identified 15,814 differentially expressed genes and 26 modules via weighted gene co-expression network analysis. Certain modules exhibited unique expression patterns specific to the different cultivars during fruit development, likely contributing to the phenotypic differences observed. Specifically, M11, M16, M22, and M23 were uniquely expressed in Tonewase, whereas M13 and M24 showed distinct patterns in Ohtanenashi. By focusing on genes with distinct expression profiles, we aimed to uncover the genetic basis of cultivar-specific traits. Our findings suggest that changes in the expression of genes associated with ethylene and cell wall pathways may drive Tonewase's earlier maturation, whereas genes related to the cell cycle within the M24 module appear crucial for Ohtanenashi's larger fruit size. Additionally, ethylene and transcription factor genes within this module may contribute to the increased fruit size observed. This study elucidates the differences in transcriptomic changes during fruit development between the two bud sport samples and their original cultivar, enhancing our understanding of the genetic determinants influencing fruit size and maturation.

Phyto-fabrication of copper oxide nanoparticles (NPs) utilizing the green approach exhibits antioxidant, antimicrobial, and antifungal activity in <i>Diospyros kaki</i> fruit

Phyto-fabrication of copper oxide nanoparticles (NPs) utilizing the green approach exhibits antioxidant, antimicrobial, and antifungal activity in <i>Diospyros kaki</i> fruit

Identification of EDNRA as the Key Biomarker for Hypercholesterolemia and Colorectal Cancer.

Some studies have investigated the role of cholesterol in the progression of colorectal cancer (CRC). However, the underlying mechanism of action is not clear. In this study, we used bioinformatics tools to elucidate the molecular mechanisms involved. We initially obtained CRC datasets from the Gene Expression Omnibus (GEO) database and hypercholesterolemia data from GeneCards and DisGeNE. Common differentially expressed genes (DEGs) were determined by using Venn diagram web tools. Next, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses using the Database for Annotation, Visualization, and Integrated Discovery (DAVID). The hub gene was identified through common expression pattern analysis and survival analysis. Finally, we conducted an immune regulatory point analysis and predicted target drugs based on the hub gene. The results of our analysis revealed 13 common DEGs, with endothelin receptor type A (EDNRA) identified as the hub gene linking hypercholesterolemia and CRC. The results of the GO analysis showed that the common DEGs were primarily associated with the G-protein coupled receptor signaling pathway, extracellular space, and receptor binding. The results of the KEGG pathway enrichment analysis indicated enrichment in pathways related to cancer and the phospholipase D signaling pathway. Additionally, we identified potential target drugs, including Podocarpus montanus, Diospyros kaki, Herba Salviae japoniae, sitaxentan, and ambrisentan. We found that EDNRA might be an underlying biomarker for both hypercholesterolemia and CRC. The predicted target drugs provide new strategies for treating CRC.

Conservation of biodiversity of subtropical crops (Citrus L., Diospyros kaki L., and Feijoa sellowiana B.) in the collections of the Federal Research Center SSC of RAS

The article examines the biodiversity of subtropical plants in the collections of the Subtropical Scientific Center of the Russian Academy of Sciences (Citrus L., Diospyros kaki L. and Feijoa sellowiana B.). Citrus fruits are represented by 144 taxa, persimmon – 27 varieties, feijoa – 13 specimens. The purpose of the study is to preserve biodiversity and replenish collections with new introduction and selection varieties. Collectible specimens are preserved alive and serve as the object of comprehensive research. Sources of economically valuable traits - early ripening, dwarfism, winter hardiness, early ripening, fruit size and productivity - have been identified and included in various breeding programs for the creation and improvement of varieties. As a result of hybridization, a diverse range of hybrid forms was obtained, including 350 forms. Two forms of Feijoa sellowiana (SHW-1: 13-11), two hybrids of C. paradise (GA-1; G-A-2) and four forms of Diospyros kaki are currently being tested. In 2023, the biodiversity of the collections was replenished with four new varieties bred at the Center. Three varieties of tangerine (‘Academichesky’, ‘Solnechny’, ‘Prince Vladimir’) and a variety of eastern persimmon (‘Zukhra’) are included in the State Register of Breeding Achievements of the Russian Federation.

Effect of Persimmon (Diospyros kaki Thunb.) Powder and Quince (Cydonia oblonga) Seed Mucilage on Physical, Chemical, Textural and Sensory Properties of Turkish Noodles

In this study, persimmon powder (PP) was substituted in concentrations of 0, 5 and 10% per 100 g of einkorn flour (EF) in Turkish noodle production. Quince seed mucilage (QSM) was used as an egg replacer at levels of 20, 30 and 40%. The cooking properties, chemical composition, color values, texture characteristics, and sensory properties of Turkish noodles were determined. The lowest cooking time (8.33 min) was found in Turkish noodles substituted with 10% PP. When 40% QSM was added to the formulation, both volume increase and water absorption values increased. Turkish noodles produced with a higher concentration of PP resulted in increased ash, crude fiber, mineral contents, especially in potassium, and total phenolic content. The lowest firmness and work of shear were obtained when PP and QSM substitutions were increased up to 10% and 40%, respectively. The sensory evaluation indicated that Turkish noodles substituted with 10% PP were greatly appreciated by the panelists in terms of taste, odor, and overall acceptability.

Pulse electric field pretreatment delays the texture deterioration of sweet persimmon (Diospyros kaki L.) during postharvest storage

The food industry is highly concerned with food processing and waste reduction sustainability. Therefore, it is essential to develop new technologies to minimize waste and extend the shelf life of products. In this study, pulsed electric field (PEF) treatments were used at 25, 50, and 100 kV/m to investigate the potential for reducing the postharvest softening of persimmons. The results showed that after storing for 28 days, the firmness loss under 50 kV/m was maintained at 58.40 ± 0.36%, whereas that in the untreated group was 87.6 ± 0.14 %. The mobility of bound water was higher in the 50 kV/m group than in the 25 kV/m and untreated groups. PEF treatment also effectively reduced electrolyte leakage. This research also showed that PEF treatment could help reduce respiration by 12.5–31.22 CO2 mL/h/kg—an effective delay of 50 kV/m for soft rot and texture deterioration. In summary, applying PEF technology was found capable of extending the shelf life of persimmons under refrigeration by up to 14 days compared with the untreated group.

A Review: Pharmacological Effect of Natural Compounds in Diospyros kaki Leaves from the Perspective of Oxidative Stress.

Oxidative stress is caused by an imbalance between reactive oxygen species and antioxidant levels. Current research suggests that oxidative stress is one of the key factors in the development of many chronic diseases, and it has been a concern for many years. Many natural compounds have been studied for their special free-radical-scavenging properties. The major chemical constituents of the leaves of Diospyros kaki are flavonoids and triterpenoids, both of which are potential antioxidants that can prevent damage caused by reactive oxygen species or reactive nitrogen species and ameliorate diseases associated with oxidative stress. In addition to the major constituents such as flavonoids and triterpenoids, the leaves of Diospyros kaki include compounds such as phenylpropanoids, alkaloids, phenolic acids, and terpenes. Studies have shown these compounds have certain antioxidant and neuroprotective activities. Experiments have shown that flavonoids or the extracts from the leaves of Diospyros kaki have a variety of good pharmacological activities, which could activate oxidative stress and mitochondrial apoptosis, inhibit the proliferation of human prostate cancer cells and induce apoptosis. It also could achieve the effect of anti-cancer cell proliferation and induce apoptosis by regulating oxidative stress. The main chemical substance of the leaves of Diospyros kaki regulating oxidative stress may be these multi-hydroxyl structure compounds. These natural products exhibit significant antioxidant activity and are an important basis for the leaves of Diospyros kaki to treat human diseases by regulating oxidative stress. This review summarizes the structural types of natural products in the leaves of Diospyros kaki and elaborates the mechanism of the leaves of Diospyros kaki in neuroprotection, anti-diabetes, renal protection, retinal degenerative diseases, and anti-cancer from a new perspective of oxidative stress, including how it supplements other pharmacological effects. The chemical constituents and pharmacological effects of the leaves of Diospyros kaki are summarized in this paper. The relationship between the chemical components in the leaves of Diospyros kaki and their pharmacological effects is summarized from the perspective of oxidative stress. This review provides a reference for the study of natural anti-oxidative stress drugs.