Jackfruit Leaf Research Articles

Effects of Fermented Jackfruit Leaf and Pulp Beverages on Gut Microbiota and Faecal Short Chain Fatty Acids Content in Sprague-Dawley Rats

Yun-Shin Sew1, Sarah Sabidi2, Shazwan Abdul Shukor1 and Soo Peng Koh2* Author Affiliations 1Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research Development Institute, Malaysia 2Food Science and Technology Research Centre, Malaysian Agricultural Research Development Institute, Malaysia Received: August 03, 2020 | Published: August 13, 2020 Corresponding author: Soo Peng Koh, Food Science & Technology Research Centre, Malaysian Agricultural Research and Development Institute, Malaysia DOI: 10.26717/BJSTR.2020.29.004817

Characterization and antifungal activity of jackfruit (Artocarpus heterophyllus Lam.) leaf extract obtained using conventional and emerging technologies.

With the current agro-food industry demands for more environmentally-friendly production, the use of natural antifungal compounds extracted by emerging technologies led to a sustainable alternative to control microorganisms. Therefore, the present work aimed to: (i) determine antifungal activity against Colletotrichum gloeosporioides and Penicillium italicum of jackfruit leaf extracts obtained by different methods, and (ii) identify compounds by HPLC-DAD-ESI-MS. Microwave (MAE), high-hydrostatic pressure (HHP) and ultrasound (UAE) assisted extractions were tested, followed by fractionation with different polarity solvents. The concentration of total soluble phenols (TSP), tannins (TT) and flavonoids (TF) were determined. Differences not only in the amounts of extracted phytochemicals were found but in the antifungal properties (MAE against P. italicum and HHP against C. gloeosporioides at 5 mg/ml) as well as in the chemical composition determined by HPLC-MS. These results suggest the possible application of jackfruit leaf extracts as a suitable postharvest antifungal treatment.

Effectivity of the jackfruit leaves infusion (Artocarpus heterophyllus l.) as a larvacide to the mortality rate of Aedes aegypti larva

Larval stage control using temephos, a chemical larvicide powder, was not completely safe. WHO recommended to use biological controls. Jackfruit leaves which contain saponins, flavonoids, and tannins can be used as alternate. This study was conducted to determine the effectivity of jackfruit leaves (Artocarpus heterophyllus L.) infusion on the mortality of Aedes aegypti larvae using post test only control group design. 600 of Aedes aegypti larvae were divided into 1 control group and 5 experiment groups that consist jackfruit leaves infusion concentrations of 4%, 8%, 12%, 16%, 20% with four times replication. The mortality of larvae was observed every 8 hours to 48 hours. The LC50 and LT50 were obtained by probit analysis were 3.842% and 2.150 hours. In the repeated Kruskal-Wallis test and Mann-Whitney, there were significant differences (p<0.05) in the mortality of Aedes aegypti larvae that died based on differences in the concentration of jackfruit leaf infusion. In the Spearman test, there were significant correlations (p<0.05) between the concentration of jackfruit leaves infusion and the mortality of Aedes aegypti larvae and the death rate of Aedes aegypti larvae. The results of the study are expected to be alternatives to chemical larvicides commonly used by the community.

FORMULASI KRIM EKSTRAK DAUN NANGKA (Artocarpus heterophyllus L.) TERHADAP BAKTERI Propionibacterium acne

Jackfruit leaf is (Artocarpus heterophyllus L) is one of the natural ingredients that can be used as a therapeutic treatment of acne. To facilitate its use the extract is made in the form of a cream. This study aims to determine the antibacterial activity of anti-acne cream jackfruit leaf extract against propionibacterium acne with concentrations of 30%, 50%, 70%, and 90%. Compounds that act as antibacterials on jackfruit leaves include flavonoids, tannins, saponins. This research method is an ANOVA one way method. The extraction method used is the maceration method. The tests include physical stability test and anti-acne activity test. Physical preparation stability test is carried out every 6 days for 3 weeks. While the anti-acne activity observation test was carried out on Propionibacterium acne bacteria. Based on the observation of organoleptic preparations of formulations 1 and 2 there was no change, while 3,4,5 there was a change. Anti- acne activity test showed that formulations 1 and 2 were more effective than formulations 3, 4, 5 with an average inhibition zone of 9.5 mm.

ANTIBACTERIAL ACTIVITY TEST OF EXTRACT ETHANOL OF JACKFRUIT LEAVES (Artocarpus heterophyllus. Lamk.) OF BACTERIA Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis and Salmonella typhi

Jackfruit leaves (Artocarpus heterophyllus Lamk.) have health benefits as an antimicrobial. The leaves contain flavonoids, tannins, saponins which act as antimicrobials. The purpose of this study was to study the potential antibacterial activity of ethanol extract of jackfruit leaves (Artocarpus heterophyllus Lamk.) On the bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi. This research conducted with an experimental method that included the collection and processing of samples, the examination of the simplicia characterization and phytochemical screening. The concentration of jackfruit leaf ethanol extract. Used was at a concentration of 500 mg mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 100 mg/mL, 50 mg/mL , 25 mg/mL, 10 mg/mL 30 mg/ml chloramphenicol and blanks. Using the disk diffusion method to measure the clear zone against the bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi. Antimicrobial inhibition of ethanol extract of jackfruit leaves against Staphylococcus aureus bacteria at a concentration of 500 mg/mL has a strong antibacterial inhibition with a diameter of 10.8 mm. The bacteria Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi at a concentration of 500 mg/mL have inhibitory power, 9.2 mm, 9.6 mm, and 8.8 mm in the medium category. Positive control chloramphenicol has powerful antibacterial inhibition with an inhibition zone diameter of 28.6 mm.

Estimation of Leaf Area of Jackfruit Through Non-destructive Method

The objective of this study was to determine mathematical equations that estimate the leaf area of jackfruit (Artocarpus heterophyllus) in an easy and non-destructive way based on linear dimensions. In this way, 300 leaves of different sizes and in good sanitary condition of adult plants were collected at the Federal Institute of Espírito Santo, Campus Itapina, located in Colatina, municipality north of the State of Espírito Santo, Brazil. Were measured The length (L) along the midrib and the maximum leaf width (W), observed leaf area (OLA), besides the product of the multiplication of length with width (LW), length with length (LL) and width with width (WW). The models of linear equations of first degree, quadratic and power and their respective R2 were adjusted using OLA as dependent variable in function of L, W and LW, LL and WW as independent variable. The data were validated and the estimated leaf area (ELA) was obtained. The means of ELA and OLA were compared by Student’s t test (5% probability) and were evaluated by the mean absolute error (MAE) and root mean square error (RMSE) criteria. The choice of the best model was based on non-significant comparative values of ELA and OLA, in addition to the closest values of zero of EAM and RQME. The jackfruit leaf area estimate can be determined quickly, accurately and non-destructively by the linear first-order model with LW as the independent variable by equation ELA = 1.07451 + 0.71181(LW).

Jackfruit leaf as an adsorbent of malachite green: recovery and reuse of the dye

Dry leaf powder of jackfruit (Actocarpus heterophyllus) was utilized for the removal of an industrial dye malachite green from the aqueous solution by the batch adsorption method. The parameters which effect on adsorption such as adsorption dose, contact time between adsorbent and the dye in solution, and pH of the solution were studied and standardized. The scanning electron microscope (SEM) photography of the jackfruit leaf powder (JLP) indicated that it had a large number of pores, spaces and empty sites. The SEM photography of used JLP shows that the dye is confidently attached on the JLP. Fourier-transform infrared spectroscopy analysis of the adsorbent confirmed the presence of different functional groups like –OH, –NH and C=O on its surface, which can be correlated with the fine adherence of the dye on the surface of the adsorbent. Moreover, the shifting of the functional group on the surface of adsorbed adsorbents indicates the affinity of the dye towards the adsorbent. The Freundlich isotherm model with the correlation coefficient (r2) value of 0.9880 is considered as a suitable absorption model for adsorption of dye. The pH of the aqueous solution was reduced from pH 7 to pH 5. This helped the recovery of the 80% of the attached dye. The present work concludes that jackfruit leaf powder can be used as an efficient adsorbent to remove malachite green from aqueous medium and the adsorbed dye can also be recovered for reuse from the surface of the adsorbent.

EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN NANGKA (Artocarpus heterophyllus L) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus

This study aims to determine whether the Ethanol Extract of Jackfruit Leaves has antibacterial properties and to determine the concentration of Ethanol Extract of Jackfruit Leaves which is useful as an antibacterial to the growth of Staphylococcus aureus bacteria. The samples tested in this study were young jackfruit leaves (not too young). Fresh jackfruit leaves weighed 1000 g, dried jackfruit leaves, 386.06 g and mashed jackfruit leaves 232.12 g and weighed 200 g. The results of research conducted from testing Ethanol Extract of Jackfruit Leaves on the growth of Staphylococcus aureus bacteria can be concluded that: Ethanol Extract of Jackfruit Leaf (Artocarpus heterophyullus L) has an antibacterial effect on the growth of Staphylococcus aureus bacteria at 50% concentration.

Removal of methylene blue (MB) from waste water by adsorption on jackfruit leaf powder (JLP) in continuously stirred tank reactor.

Dye contaminated waste water possess serious threat to the goal of reaching sustainable environment. Bio adsorbents can be utilized very effectively in removing dye ingredient from wastewater due to their high kinetics and capacity. The study was focused on observing bio adsorbent (jackfruit leaf powder) activity for Methylene Blue adsorption in a Continuous Stirred Tank Reactor (CSTR) for wastewater treatment.Batch and kinetic study of adsorbent were performed. The maximum adsorption capacity at equilibrium was found to be 271 mg/g and adsorption followed Langmuir isotherm. The kinetics study provided evidence of instantaneous adsorption while the zero point charge of the adsorbent was 3.9. The FTIR of the JLP-MB system was carried out to explain the adsorption phenomena with representing groups. The CSTR study was performed under various parameters such as, effect of flow rate, adsorbent dosage, CSTR arrangement and initial concentration. The dye stream first treated in 1st reactor and then its effluent passes into the 2nd reactor and treated again. It was found that the single CSTR removes 30-45 % of the dye depending on the initial concentration, while the series effect removes 70-85 % of the dye overall.

Sustainable bioremediation of Cd(II) from aqueous solution using natural waste materials: Kinetics, equilibrium, thermodynamics, toxicity studies and GA-ANN hybrid modelling

Abstract The paper aims to provide efficient and affordable means for pollution abatement to MSME sector. In this work, the performance of some naturally available bio-waste materials, viz., leaves of jackfruit, mango and rubber plants have been attempted as biosorbents for remediation of toxic Cd(II) from aqueous solution. The biomasses have been characterized to find out the surface morphology, active surface area, presence of characteristic surface groups etc. which facilitate the biosorption process. Batch experiments are conducted to see the effects of operating parameters, viz., aqueous phase pH, initial Cd(II) concentration, adsorbent dose, time, temperature for Cd(II) ion removal onto these green biomaterials. The bioremediation mechanism was strongly pH dependent, spontaneous and followed second-order kinetics. Mass transfer, intra-particle diffusion and chemical adsorption controlled the process. Jackfruit leaf showed the best sorption performance by removing 98.72% Cd(II) from 20 mg L−1 aqueous solution at a dose of 5 g L−1 and the equilibrium adsorption capacity of 20.37 mg g−1. The bio-sorbents performed satisfactory when tested against the industrial wastewater. The toxicity effect of the cadmium ion was analysed on living animal cell and they showed morphological alteration of RBC along with clumped appearance. The cell alteration intensity reduced with the treated effluent. The natural adsorbents have comparable adsorption capacity of other green adsorbents used by different researchers. Modelling of the complex sorption process has been performed using hybrid artificial intelligence (GA-ANN) technique to predict the metal ion removal efficiency accurately and obtained results have good agreement with the experimental data with correlation coefficient (R) ranging from 0.97–0.99. All these findings have manifested application of jackfruit, rubber and mango leaves for removal of Cd(II) ions in an environmentally sustainable and friendly way.

Potensi Ekstrak Daun Nangka sebagai Biolarvasida Nyamuk Culex sp

ABSTRACTMosquitoes are type of insect that acts as a disease vector. One of them is Culex sp mosquitos which their existence is many around us. In Indonesia there are still a lot of disease cases that caused by Culex sp mosquitos, one of them is filariasis disease. The efforts to control thecurrent population of Culex sp mosquitoes mostly use chemical insecticides. The control becomes difficult because of the resistance to chemical insecticides and adversely affects the environment. This study aims to determine the potential of jackfruit leaf extract as biolarvasida against Culex sp. The type of this study was post test onlycontrolled group design, using 625 larvae Culex sp instar III, divided into 5 groups (negative control group, 0.25% extract, 0.50% extract, 0.75% extract, extract and 1% extract). Observation was performed 24 hoursafter the treatment and counted the number of larvae death.The data were analyzed by Kruskal Wallis test followed by Post Hoc Mann-Whitney U test to determine the killing power of leafy leaf extractusing Probit analysis. The results of data analysis in this study were thedeath rate percentage of larvae after 24 hours treatment was 0% incontrol, 44% in 0.25%extract, 52% in0.50% extract, 60% in 0.75%extract,while in the extract group of 1%, the number of larval deaths was 72%. Itobtained results with significant differencein some treatment groups thatwere concentrations of 0.25% - 1%. Probit analysis showed that LC50 wasat 0.382% concentration.From results of the studyit can be concluded that jackfruit leaf extract(Artocarpus heterophyllus Lamk) was potential as Culex sp mosquitobiolarvacide. So it is necessary to conduct further research on the effectsof compounds that contained in other plant parts such as flowers, stemsand roots which will be expected to have a function asbiolarvasida.Keywords: Leaf Jackfruit, Biolarvasida, Mosquito Culex sp

APPLICATION OF USE JUICE LEAF JACKFRUIT (Artocarpus heterophyllus) AS NATURAL DISINFECTANT IN POULTRY INCUBATOR ON DECREASING NUMBER AND ZONA RESISTOR OF BACTERIA

ABSTRACTThis study aims to examine the effect of the use of jackfruit leaf juice as a natural disinfectant to decrease the amount and the inhibition of bacteria in incubator. The method used in this research is experimental method in laboratory using Completely Randomized Design with 3 treatments and 6 replications. The three treatments are P1 = juice of jackfruit leaf with dilution 75% (25% concentration), P2 = juice of jackfruit leaf with 50% dilution (50% concentration), and P3 = juice of jackfruit leaves with 25% dilution (75% concentration). Data were analyzed by means of verbalization and to know the difference between treatments was done by Duncan test. The results showed that the juice solution of jackfruit leaves contain phenol compounds namely flavonoids, saponins and tannin compounds which are anti microbial substances. Treatment of P3 (75%) decreased the number of bacteria as much as 82.54% and the widest drag 0.3 cm for bacteria coccus and 0.5 cm for bacteria bacil.

Biosorption of chromium (VI) from aqueous solutions and ANN modelling.

The use of sustainable, green and biodegradable natural wastes for Cr(VI) detoxification from the contaminated wastewater is considered as a challenging issue. The present research is aimed to assess the effectiveness of seven different natural biomaterials, such as jackfruit leaf, mango leaf, onion peel, garlic peel, bamboo leaf, acid treated rubber leaf and coconut shell powder, for Cr(VI) eradication from aqueous solution by biosorption process. Characterizations were conducted using SEM, BET and FTIR spectroscopy. The effects of operating parameters, viz., pH, initial Cr(VI) ion concentration, adsorbent dosages, contact time and temperature on metal removal efficiency, were studied. The biosorption mechanism was described by the pseudo-second-order model and Langmuir isotherm model. The biosorption process was exothermic, spontaneous and chemical (except garlic peel) in nature. The sequence of adsorption capacity was mango leaf > jackfruit leaf > acid treated rubber leaf > onion peel > bamboo leaf > garlic peel > coconut shell with maximum Langmuir adsorption capacity of 35.7mgg-1 for mango leaf. The treated effluent can be reused. Desorption study suggested effective reuse of the adsorbents up to three cycles, and safe disposal method of the used adsorbents suggested biodegradability and sustainability of the process by reapplication of the spent adsorbent and ultimately leading towards zero wastages. The performances of the adsorbents were verified with wastewater from electroplating industry. The scale-up study reported for industrial applications. ANN modelling using multilayer perception with gradient descent (GD) and Levenberg-Marquart (LM) algorithm had been successfully used for prediction of Cr(VI) removal efficiency. The study explores the undiscovered potential of the natural waste materials for sustainable existence of small and medium sector industries, especially in the third world countries by protecting the environment by eco-innovation.

Green mediated synthesis of zinc oxide nanoparticles for the photocatalytic degradation of Rose Bengal dye

Zinc oxide (ZnO) nanoparticles were synthesised by a simple method using leaf extract of jackfruit (Artocarpus heterophyllus). The phytochemicals present in the leaf act as stabilising agents. The green synthesised ZnO nanoparticles were characterized by X-ray diffraction (PXRD), Scanning Electron microscopy (FESEM), Energy dispersive spectroscopy (EDS) and Transmission electron microscopy (TEM). XRD pattern confirmed the formation of crystalline ZnO nanoparticles. The SEM images showed porous, sponge-like agglomerated structures. TEM analysis showed the ZnO nanoparticles formed to have hexagonal wurtzite structure and the particles ranging between 15 and 25nm. The green synthesised ZnO nanoparticles exhibited excellent photodegradation efficiency (>80%, 0.24g/L, 1h) against Rose Bengal dye, a main water-pollutant released by the textile industries. The characterisation results confirmed that the ZnO nanoparticles can be efficiently synthesised using jackfruit leaf extract as stabiliser and the photodegradation results proved the efficiency of the green synthesised ZnO nanoparticles for the degradation of Rose Bengal dye.

Performance of a submerged adsorption column compared with conventional fixed-bed adsorption

Submerged adsorption (SA) process has been explored in this context for the first time as a dynamic and effective approach, comparable to the conventional fixed-bed adsorption. Various design parameters like breakthrough time (tb), adsorption capacity (qeq), and effective bed height (HB) of the adsorption columns were determined for different flow patterns, e.g. up-flow and down-flow. A fixed bed of jackfruit (Artocarpus heterophylus) leaf powder, already proved by our group as an efficient, cost-effective adsorbent for the removal of methylene blue from water in continuous mode using fixed-bed column, was selected for this study. The design basis of the adsorption columns were regarded as different bed depths (HT = 5–10 cm), flow rates (Q = 20–60 mL/min), and initial dye concentrations (C0 = 300–500 mg/L), for the experiment. With decreasing flow rates as well as increasing bed height and initial dye concentration for any flow arrangement, adsorption capacity of the column increased as the breakthrough time and usable bed height increased. Moreover, adsorption columns with up-flow showed better performance than down-flow pattern may be due to increasing qeq and HB up to 40 and 20%, respectively. Comparing design parameters of SA column to conventional FBA found that qeq increased up to 22% as well as HB and tb increased corresponding to 9–14% and 20–86%, respectively. The experimental data were fitted with Thomas model and correlated with theoretical breakthrough curves, which pointed out the evidence of the effective design approach.

Adsorption characteristics of jackfruit leaf powder for the removal of Amido black 10B dye

Adsorption characteristics of jackfruit leaf powder for the removal of Amido black 10B dye

STUDI TENTANG BENTUK, MOTIF DAN TEKNIK KRIYA PERAK KOTO GADANG MINANGKABAU

Silver is a white shiny metal. It is used as daily needs as well as decoration. The silver handicraft in Koto Gadang district have been well-known since long time ago. The things made by each handycraft centers are almost the same so that it is difficult to create interesting shapes for consumers. Beside that, the technique used is still a simple one, and the motives applied to the handicraft are only little developed. Therefore, this research tries to describe several things: 1) To identify silver handicraft product shapes; 2) To identify the name of the motives being developed; 3) To explain the production technique of silver handicraft. This research is conducted in Koto Gadang; using quantitative and qualitative sampling. The result of this research is silver handicraft shapes, which involve various kinds of earring, bracelet, brooch, ring, necklace, pendant, and miniature, as well as ornament for wedding ceremnny, among other things are various shapes of necklace, ring, and ear-plug. To make handicraft, chisel, kikir (serrated iron for smoothing something), plait, and sickle. The motives applied on the products are cucumber leaf, padek leaf, jackfruit leaf, "H" leaf Lombok, bataro, straight lines, winding, parallel line, bamboo plait, and fish scale. Key words: Koto Gadang, silver handicraft, shape, motive, and technical handicraft study

Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein)

<p>Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,<br />albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)<br />albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins with<br />bovine serum albumin. In the second stage there was the determination of protein-percipitable phenolics compound and protein content using Lowry method. The data showed total phenol content was 10.63%, total tannin was 7.08%, and<br />condensed tannins 5.57%. The optimum capacity phenolic compound to bind protein of bovine serum albumin was 5.71+0.18 mg BSA/100 mg dry matter of jackfruit leaf, while 1 g tannin could bind 23.149 g BSA or 1 g condensed<br />tannin could bind 28.885 g BSA.</p><p><br />(Key words: Tannin, Total phenol, Total tannin, Condensed tannin, Bovine serum albumin)<br /><br /></p>

ANTIOKSIDAN DALAM MASAKAN MINANG DAN POTENSI PROTEKTIF TERHADAP RISIKO PENYAKIT KARDIOVASKULAR

Cardiovascular disease (CVD) is aprimary cause of death in Indoensia nowaday. Development of CVD is caused by some determinant factors, as well as nutrient and non-nutrient intake to result of oxidative stress in the body. Antioxidants, such as vitamin E, beta-caroten, vitamin C andflavonoid, are able to neutralize the effect of free radical from oxidative stress and to influence lipid plasma. The objective of this study was to know the level of antioxidant intake and its association with blood lipid among Minangkabau ethnic. A cross sectional study was conducted in Padang ciry. The study population was adult, age 30 -65 years old, and sampling technique was multistage random sampling, and sample size is 215 respondents. Data collected were demographic characterisitics, food intake using semi quantitative food frequency questionnaire (SMFFQ) and anthropometric measurements. Blood samples were collected to assess plasma lipid profile and level of flavonoid. Data was analyzed with SPSS version 12 and modifiedNutriSurvey Indonesia. The results showed that the average intake of flkaroten was 15.0 ( SD 6. 7) mg/dcry, vitamin C was 66. 3 (SD 30.6) mg/day, vitamin E was 17,8 (SD 11.8) mg/day and flavonoid was 105.0 (SD 48.1) mg/day. The level, offlavonoid from Minang traditional food was highfrom "gulai" which consisted of some vegetables, such as cassava leaf and jack fruit leaf, and from "samba lado " which consisted of red pepper, union, garlic andsame traditional spices. The mean of total cholesterol, LDL cholesterol were above normal but the mean of HDL cholesterol, trigliserides, and ratio of cholesterol to LDL/HDL were within normal values. The result showed significant association between antioxidants intake from traditional Minangfood with some lipid profile after adjusted for some counfounding factors such as gender and age. It was concluded that high level of antioxidant intake from traditional "Minang" food with high-level fiber intake was a potentialprotective factor for CVD risk in ethnic Minang communities.

Adsorption of methylene blue from aqueous solution by jackfruit ( Artocarpus heteropyllus) leaf powder: A fixed-bed column study

Continuous fixed-bed studies were undertaken to evaluate the efficiency of jackfruit leaf powder (JLP) as an adsorbent for the removal of methylene blue (MB) from aqueous solution under the effect of various process parameters like bed depth (5–10 cm), flow rate (30–50 mL/min) and initial MB concentrations (100–300 mg/L). The pH at point of zero charge (pH PZC) of the adsorbent was determined by the titration method and a value of 3.9 was obtained. A FTIR of the adsorbent was done before and after the adsorption to find the potential adsorption sites for interaction with methylene blue molecules. The results showed that the total adsorbed quantities and equilibrium uptake decreased with increasing flow rate and increased with increasing initial MB concentration. The longest breakthrough time and maximum MB adsorption were obtained at pH 10. The results showed that the column performed well at low flow rate. Also, breakthrough time and exhaustion time increased with increasing bed depth. The bed-depth service time (BDST) model and the Thomas model were applied to the adsorption of MB at different bed depths, flow rates, influent concentrations and pH to predict the breakthrough curves and to determine the characteristic parameters of the column that are useful for process design. The two model predictions were in very good agreement with the experimental results at all the process parameters studied indicating that they were very suitable for JLP column design.