ITS Types Research Articles

<p><strong>Molecular phylogenetic data on reticulate evolution in the genus <em>Barbilophozia</em> Löske (Anastrophyllaceae, Marchantiophyta) and evidence of non-concerted evolution of rDNA in <em>Barbilophozia</em> <em>rubescen</em>s allopolyploid</strong></p>

Phylogeny of the genus Barbilophozia inferred from ITS1-2 nrDNA, trn L-F and trn G-intron cpDNA provides different evolutionary scenarios within the genus. ITS1-2 tree placed B. barbata and B. lycopodioides in sister position, whereas from both cpDNA loci morphologically quite distinctive B. barbata is separated from B. hatcheri and B. lycopodioides. The significant differences in nucleotide sequences suggest that B. lycopodioides and B. hatcheri are clearly separated species. The poorly known species— B. rubescen s—has originated through hybridization of B. barbata and B. hatcheri. The occurrence of both two parental types of ITS in hybrid accessions indicate a non-completeness of concerted evolution in these cases. The highest haplotype diversity is found for ITS1-2 that could be caused by recombination, whereas trn L-F and trn G are characterized only by several haplotypes. The haplotypes distribution is weakly supported from geographical evidence.

Extreme habitats that emerged in the Pleistocene triggered divergence of weedy Youngia (Asteraceae) in Taiwan

Weeds with broad distributions and large morphological variation are challenging for systematists and evolutionarily intriguing because their intensive dispersal would likely prevent local morphological differentiation. Study on weeds will help to understand divergence in plants unlikely to be affected by geographical and ecological barriers. We studied Youngia japonica based on nrDNA and cpDNA sequences. This is a widespread native in Asia and invasive worldwide; nevertheless, three subspecies (japonica, longiflora, and formosana) and an undescribed variant occur in Taiwan.Bayesian and the most parsimonious phylogenies revealed that subspecies longiflora is a different linage and independently arrived in Taiwan during the Pleistocene via land connection to the Asian Continent. Bayesian time estimation suggested that Youngia in Taiwan diverged in the lower Pleistocene or more recently. Extreme habitats that emerged in the Pleistocene, i.e., cold mountain ranges for subspecies formosana and xeric, raised coral reefs for the undescribed Youngia variant probably had triggered the divergence.Components of Youngia in Taiwan are not monophyletic; a coalescent-based test suggested incomplete lineage sorting. Nevertheless, the samples within each taxon share unique morphological features suggesting a common gene pool and each taxon has different dominant ITS and/or cpDNA types; these conditions suggest ongoing process toward monophyly via coalescent processes and support the delimitation of intraspecific taxa.

Two different and functional nuclear rDNA genes in the abalone Haliotis tuberculata: tissue differential expression

Analysis of the 18S rDNA sequences of Haliotis tuberculata tuberculata and H. t. coccinea subtaxa identified two different types of 18S rDNA genes and ITS1 regions. These two different genes were also detected in H. marmorata, H. rugosa and H. diversicolor that are separated from H. tuberculata by 5-65 mya. The mean divergence value between type I and type II sequences ranged from 7.25% for 18S to 80% for ITS1. ITS1 type II is homologous with the ITS1 consensus sequences published for many abalone species, whereas ITS1 type I presented only minor homology with a unique database entry for H. iris ITS1. A phylogenetic analysis makes a clear separation between type I and type II ITS1 sequences and supports grouping H. t. tuberculata, H. t. coccinea and H. marmorata together. The two subtaxa do not show any significant differences between the homologous 18S rDNA sequences. A general structure of the ITS1 transcript was proposed, with four major helices for the two types. The two genes were expressed and, for the first time, a putative differential expression of ITS1 type I was detected in the gills, digestive gland and gonads whereas ITS1 type II was expressed in all tissues.

Identification and Targeted Cultivation of Abundant Novel Freshwater Sphingomonads and Analysis of Their Population Substructure

Little is known with respect to bacterial population structures in freshwater environments. Using complementary culture-based, cloning, and high-throughput Illumina sequencing approaches, we investigated microdiverse clusters of bacteria that comprise members with identical or very similar 16S rRNA gene sequences. Two 16S rRNA phylotypes could be recovered by cultivation in low-nutrient-strength liquid media from two lakes of different trophic status. Both phylotypes were found to be physiologically active in situ throughout most of the year, as indicated by the presence of their rRNA sequences in the samples. Analyses of internal transcribed spacer (ITS1) sequences revealed the presence of seven different sequence types among cultured representatives and the cloned rrn fragments. Illumina sequencing yielded 8,576 ITS1 sequences that encompassed 15 major and numerous rare sequence types. The major ITS1 types exhibited distinct temporal patterns, suggesting that the corresponding Sphingomonadaceae lineages occupy different ecological niches. However, since strains of the same ITS1 type showed highly variable substrate utilization patterns, the potential mechanism of niche separation in Sphingomonadaceae cannot be explained by substrate utilization alone and may be related to other traits.

Aligning IT, strategic orientation and organizational structure

Purpose – The purpose of the paper is to examine and analyze the alignment between (information technology) IT, strategic orientation (SO) and organizational structure (OS) and their impact on firm performance (FP).Design/methodology/approach – A theoretical framework is proposed regarding the constructs of IT, SO and OS. A model incorporating these three constructs is examined and their impact on FP is assessed using structural equation modeling (SEM). The sample data from 295 firms were obtained through structured questionnaires.Findings – The results of the SEM support the hypothesis that the alignment between IT, SO and OS significantly affects FP.Research limitations/implications – Non‐financial and intangible performance measurements are not included and the sample is not homogeneous.Practical implications – This study suggests that managers should choose the appropriate level and type of IT, depending on a firm's structure and SO, in order to benefit from the advantages of IT usage and achieve high...

Infection Dynamics Vary between Symbiodinium Types and Cell Surface Treatments during Establishment of Endosymbiosis with Coral Larvae

Symbioses between microbes and higher organisms underpin high diversity in many ecosystems, including coral reefs, however mechanisms underlying the early establishment of symbioses remain unclear. Here we examine the roles of Symbiodinium type and cell surface recognition in the establishment of algal endosymbiosis in the reef-building coral, Acropora tenuis. We found 20–70% higher infection success (proportion of larvae infected) and five-fold higher Symbiodinium abundance in larvae exposed to ITS-1 type C1 compared to ITS-1 type D in the first 96 h following exposure. The highest abundance of Symbiodinium within larvae occurred when C1-type cells were treated with enzymes that modified the 40–100 kD glycome, including glycoproteins and long chain starch residues. Our finding of declining densities of Symbiodinium C1 through time in the presence of intact cell surface molecules supports a role for cell surface recognition molecules in controlling post-phagocytosis processes, leading to rejection of some Symbiodinium types in early ontogeny. Reductions in the densities of unmodified C1 symbionts after 96 h, in contrast to increases in D symbionts may suggest the early initiation of a winnowing process contributing to the establishment of Symbiodinium D as the dominant type in one-month old juveniles of A. tenuis.

The long-term consequences of hybridization between the two Daphnia species, D. galeata and D. dentifera, in mature habitats

BackgroundEcological specializations such as antipredator defense can reinforce morphological and distributional divergence within hybridizing species. Two hybridizing species of Daphnia (D. galeata and D. dentifera) are distributed in both Japan and North America; however, these populations have a longer history in Japan than in North America due to the differing impact of the last glaciation on these two regions. We tested the hypothesis that this longer coexistence in Japan would lead to extensive genetic admixture in nuclear and mitochondrial DNA whilst the distinct morphological traits and distributional patterns would be maintained.ResultsThe high level of correspondence among morphological traits, distribution, and mitochondrial and nuclear DNA types for the specimens with D. dentifera mtDNA indicated that the species distinction has been maintained. However, a discordance between mtDNA and nuclear ITS-1 types was observed for most specimens that had D. galeata mtDNA, consistent with the pattern seen between the two species in North America. This observation suggests nuclear introgression from D. dentifera into D. galeata without mitochondrial introgression.ConclusionsThe separation of morphological traits and distribution ranges of the two hybridizing species in Japan, as well as in North America, has been maintained, despite large differences in climatic and geographical histories of these two regions. Variations in environmental factors, such as predation pressure, might affect maintenance of the distribution, although the further studies are needed to confirm this.

Finding the Role of Time Lags in Radio Frequency Identification Investment

Radio Frequency Identification (RFID) is a highly beneficial technological advancement which could eventually change the way of doing business. However, the difficulty in assessing the potential benefits of RFID has been delayed RFID related investment decisions within organizations. Especially, time lag, or payback time from RFID investment, has been one of key barriers in RFID investment. This study provides a conceptual framework for time lags in RFID to enhance the understanding of RFID pay-offs. Time lags are determined by IT type and a firm’s IT capability. Therefore, it takes longer for firms to realize the value of RFID since it requires more efforts and changes to implement and utilize. Also the expected time lags of RFID may be modified by the level of IT capability of each firm. The stronger IT capability the firm has, the shorter time lags the firm experiences.

CID of singly charged antioxidants applied in lubricants by means of a 3D ion trap and a linear ion trap–Orbitrap mass spectrometer

The aim of this study was to investigate the fragmentation behavior induced by low-energy collision-induced dissociation (LE-CID) of four selected antioxidants applied in lubricants, by two different types of ion trap mass spectrometers: a three-dimensional ion trap (3D-IT) and a linear IT (LIT) Orbitrap MS. Two sterically hindered phenols and two aromatic amines were selected as model compounds representing different antioxidant classes and were characterized by positive-ion electrospray ionization (ESI) and LE-CID. Various types of molecular ions (e.g. [M](+•) , [M + H](+) , [M + NH(4) ](+) or [M + Na](+) ) were used as precursor ions generating a significant number of structurally relevant product ions. Furthermore, the phenolic compounds were analyzed by negative-ion ESI. For both IT types applied for fragmentation, the antioxidants exhibited the same unusual LE-CID behavior: (1) they formed stable radical product ions and (2) CC bond cleavages of aliphatic substituents were observed and their respective cleavage sites depended on the precursor ion selected. This fragmentation provided information on the type of structural isomer usually not obtainable for branched aliphatic substituents utilizing LE-CID. Comparing the two instruments, the main benefit of applying the LIT-Orbitrap was direct access to elemental composition of product ions enabling unambiguous interpretation of fragmentation trees not obtainable by the 3D-IT device (e.g. loss of isobaric neutrals). It should be emphasized that the types of product ions formed do not depend on the type of IT analyzer applied. For characterizing degradation products of antioxidants, the LIT-Orbitrap hybrid system, allowing the determination of accurate m/z values for product ions, is the method of choice.

Morphological and molecular characterization of two ITS groups of Erysiphe (Erysiphales) occurring on Syringa and Ligustrum (Oleaceae)

ITS sequences determined for 53 Erysiphe specimens on Syringa and Ligustrum collected in Europe, East Asia, and North and South America were divided into two ITS groups, S and K types. Phylogenetic analysis showed that these two ITS types do not share a common ancestor and form separate clades. The K type on Ligustrum was identified as Erysiphe ligustri based on the three-dimensional branching pattern of appendages. Morphological observations showed that there are some morphological differences—pigmentation of appendages and number of ascospores per ascus—between the S and K types on Syringa. Based on these morphological observations, the S and K types on Syringa were identified as E. syringae and E. syringae-japonicae, respectively. The recent abundant production of chasmothecia by lilac powdery mildew in Europe was caused by E. syringaejaponicae introduced from East Asia. DNA sequence analyses of the rDNA ITS region and the 28S rDNA, tub2, CYP51, and Chs1 genes did not support an interspecific hybrid origin for E. syringae-japonicae. Haplotype analysis suggested that E. syringae originated in North America and independently migrated to East Asia and Europe/South America.

Variation in the nrDNA ITS sequences of some powdery mildew species: do routine molecular identification procedures hide valuable information?

During the past years, nrDNA ITS sequences have supported the identification of many powdery mildew fungi because comprehensive analyses showed that differences in these sequences have always correlated with the delimitation of different species and formae speciales of the Erysiphales. Published data, obtained using direct sequencing of the PCR products, suggested that even one to five nucleotide differences in the ITS sequences delimit different, albeit closely related, species, and/or indicate differences in host range patterns. Here we show that such differences in the ITS sequences can be detected even in a single sample of a powdery mildew fungus. We sequenced the ITS region in 17 samples, representing six powdery mildew species, both directly and after cloning the PCR products. Among these, samples of O. longipes exhibited two or three, samples of O. neolycopersici three or four, those of an Oidium sp. from Chelidonium majus up to seven, and a sample of another Oidium sp. from Passiflora caerulea two different ITS types determined after cloning. No ITS nucleotide polymorphisms were found in samples of O. lycopersici and Erysiphe aquilegiae. This suggests that some powdery mildew taxa are more variable at the ITS level than others. Thus, although the ITS sequences determined by direct sequencing represent robust data useful in delimitation and phylogenetic analysis of distinct species of the Erysiphales, these need to be used with precaution, and preferably determined after cloning, especially when dealing with closely related taxa at species and sub-species levels. With this method a hitherto undetected genetic diversity of powdery mildews can be revealed.

Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae)

We investigated the effect of elevated partial pressure of CO2 (pCO2) on the photosynthesis and growth of four phylotypes (ITS2 types A1, A13, A2, and B1) from the genus Symbiodinium, a diverse dinoflagellate group that is important, both free‐living and in symbiosis, for the viability of cnidarians and is thus a potentially important model dinoflagellate group. The response of Symbiodinium to an elevated pCO2 was phylotype‐specific. Phylotypes A1 and B1 were largely unaffected by a doubling in pCO2; in contrast, the growth rate of A13 and the photosynthetic capacity of A2 both increased by ~ 60%. In no case was there an effect of ocean acidification (OA) upon respiration (dark‐ or light‐dependent) for any of the phylotypes examined. Our observations suggest that OA might preferentially select among free‐living populations of Symbiodinium, with implications for future symbioses that rely on algal acquisition from the environment (i.e., horizontal transmission). Furthermore, the carbon environment within the host could differentially affect the physiology of different Symbiodinium phylotypes. The range of responses we observed also highlights that the choice of species is an important consideration in OA research and that further investigation across phylogenetic diversity, for both the direction of effect and the underlying mechanism(s) involved, is warranted.

The role of deep reefs in shallow reef recovery: an assessment of vertical connectivity in a brooding coral from west and east Australia

Approximately one quarter of zooxanthellate coral species have a depth distribution from shallow waters (<30 m) down to mesophotic depths of 30-60 m. The deeper populations of such species are less likely to be affected by certain environmental perturbations, including high temperature/high irradiance causing coral bleaching. This has led to the hypothesis that deep populations may serve as refuges and a source of recruits for shallow reef habitats. The extent of vertical connectivity of reef coral species, however, is largely unquantified. Using 10 coral host microsatellite loci and sequences of the host mtDNA putative control region, as well as ribosomal DNA (rDNA) ITS2 sequences of the coral's algal endosymbionts (Symbiodinium), we examine population structure, connectivity and symbiont specificity in the brooding coral Seriatopora hystrix across a depth profile in both northwest (Scott Reef) and northeast Australia (Yonge Reef). Strong genetic structuring over depth was observed in both regions based on the microsatellite loci; however, Yonge Reef exhibited an additional partitioning of mtDNA lineages (associated with specific symbiont ITS2 types), whereas Scott Reef was dominated by a single mtDNA lineage (with no apparent host-symbiont specificity). Evidence for recruitment of larvae of deep water origin into shallow habitats was found at Scott Reef, suggesting that recovery of shallow water habitats may be aided by migration from deep water refuges. Conversely, no migration from the genetically divergent deep slope populations into the shallow habitats was evident at Yonge Reef, making recovery of shallow habitats from deeper waters at this location highly unlikely.

Photosystem II breakdown induced by reactive oxygen species in freshly-isolated Symbiodinium from Montipora (Scleractinia; Acroporidae)

Freshly-isolated Symbiodinium (FIS) have been used to study cnidarian–alga symbiosis based on the assumption that their physiological performance is comparable to that of the algae in hospite. This assumption was tested with 15 species of scleractinian corals, using pulse-amplitude modulation (PAM) chlorophyll a (chl a) fluorescence to compare maximum quantum yields (Fv/Fm) of photosystem II (PSII) in Symbiodinium in hospite and after isolation in seawater. FIS from Montipora spp. exhibited rapid and dramatic decreases (by up to >95%) in PSII activity within 30 min of isolation. In contrast, PSII activities of FIS from 8 other coral species decreased by only 5 to 21% after >4 h in seawater. To investigate possible reasons for this variation, the variation in Symbiodinium genetic type (ITS-2 types), transmission modes and several physiological indices were considered. The rapid loss of PSII activity in FIS from Montipora spp. was not correlated with Symbiodinium ITS-2 type, the mode of symbiont transmission, or ionic regulation capability, nor could consistent chemical effects in host extracts be demonstrated. PSII inactivation was correlated with increased levels of reactive oxygen species (ROS), degradation of pigments in peridinin-chl a protein, and chloroplast disruption, indicating that the FIS was under physiological collapse. The specific mechanism(s) causing Montipora-associated Symbiodinium to fail during the isolation methods used here remain unknown. However, these data indicate that care should be taken when using FIS to represent Symbiodinium in hospite, especially when comparing different species of corals.

Morphological and molecular characterization of two ITS groups of &lt;i&gt;Erysiphe&lt;/i&gt; (Erysiphales) occurring on &lt;i&gt;Syringa&lt;/i&gt; and &lt;i&gt;Ligustrum&lt;/i&gt; (Oleaceae)

ITS sequences determined for 53 Erysiphe specimens on Syringa and Ligustrum collected in Europe, East Asia, and North and South America were divided into two ITS groups, S and K types. Phylogenetic analysis showed that these two ITS types do not share a common ancestor and form separate clades. The K type on Ligustrum was identified as Erysiphe ligustri based on the three-dimensional branching pattern of appendages. Morphological observations showed that there are some morphological differences—pigmentation of appendages and number of ascospores per ascus—between the S and K types on Syringa. Based on these morphological observations, the S and K types on Syringa were identified as E. syringae and E. syringae-japonicae, respectively. The recent abundant production of chasmothecia by lilac powdery mildew in Europe was caused by E. syringaejaponicae introduced from East Asia. DNA sequence analyses of the rDNA ITS region and the 28S rDNA, tub2, CYP51, and Chs1 genes did not support an interspecific hybrid origin for E. syringae-japonicae. Haplotype analysis suggested that E. syringae originated in North America and independently migrated to East Asia and Europe/South America.

IT Governance and Types of IT Decision Makers in German Hospitals

Growing expenses for health care services in hospitals stress the potential of a well applied IT governance for cost reduction, productivity gains and a possible source for competitive advantages. The underlying explorative study analyzes the current status of IT governance through a survey among 206 IT decision makers in German hospitals. The quantitative analyses show that the most important requirements of IT managers for IT are the optimization and standardization of processes, that IT investments shift from administrative to medical IT applications, and that private hospitals display relatively higher IT budgets than do non-profit or public hospitals based on financial turnover. Further, two types of future IT decision makers are empirically identified. The types differ regarding their future role as initiators for process optimization and regarding the degree of involvement in strategic decision making.

Marketonomics of Cloud Computing

According to Theodore levitt (2006), the change is imminent and irresistible. The world has seen many volatile times and there might be some more waiting in the queue for the business community. The top priority by businesses should be given towards perseverance of competitive advantage that is resistant to change. IT can be imagined as an injection full of steroids to boost the performance of a firm. If one firm or industry after adopting certain type of IT, reaps benefits, then the adoption process immediately starts. Cloud computing is a collection of internet based service that has vast amount of capabilities. From an economic development perspective cloud computing can not be ignored. The opportunities of adopting cloud computing in government bodies, SMEs are numerous that leads to economic development and creation of large, better skilled, digitally literate workers pool. This paper tries to give the IT investment behavior of the firms in volatile times and also emphasizes the advantages of cloud computing to government organization, SMEs, rural population and on the whole economy of a nation.

Semi-permeable species boundaries in the coral genus Madracis: Introgression in a brooding coral system

Introgressive hybridization is described in several phylogenetic studies of mass-spawning corals. However, the prevalence of this process among brooding coral species is unclear. We used a mitochondrial (mtDNA: nad5) and two nuclear (nDNA: ATPSα and SRP54) intron markers to explore species barriers in the coral genus Madracis and address the role of hybridization in brooding systems. Specimens of six Caribbean Madracis morphospecies were collected from 5 to 60 m depth at Buoy One, Curaçao, supplemented by samples from Aruba, Trinidad & Tobago and Bermuda. Polymerase chain reaction and denaturing gradient gel electrophoresis were coupled to detect distinct alleles within single colonies. The recurrent nDNA phylogenetic non-monophyly among taxa is only challenged by Madracis senaria, the single monophyletic species within the genus. nDNA AMOVAs indicated overall statistical divergence (0.1% significance level) among species but pairwise comparisons of genetic differentiation revealed some gene exchange between Madracis taxa. mtDNA sequences clustered in two main groups representing typical shallow and deep water Madracis species. Madracis pharensis shallow and deep colonies (with threshold at about 23–24 m) clustered in different mtDNA branches, together with their depth-sympatric congenerics. This divergence was repeated for the nDNA ( ATPSα) suggestive of distinct M. pharensis depth populations. These matched the vertical distribution of the dinoflagellate symbionts hosted by M. pharensis, with Symbiodinium ITS2 type B7 in the shallows but type B15 in the deep habitats, suggesting symbiont-related disruptive selection. Recurrent non-monophyly of Madracis taxa and high levels of shared polymorphism reflected in ambiguous phylogenetic networks indicate that hybridization is likely to have played a role in the evolution of the genus. Using coalescent forward-in-time simulations, lineage sorting alone was rejected as an explanation to the SRP54 genetic variation contained in Madracis mirabilis and Madracis decactis (species with an old fossil record), showing that introgressive hybridization has taken place between these species, either directly or through the gene pool of other Madracis taxa. Madracis widespread non-monophyly and the absence of statistical divergence between some species suggest that introgressive hybridization plays an important role in the evolution of the genus. Different reproductive traits and symbiont signatures of taxa forming distinct genetic clusters also point to the same conclusion. We suggest that Madracis morphospecies remain recognizable because introgressive hybridization is non-pervasive and/or because disruptive selection is in action.

Assessing the suitability of process and information technology in supporting tacit knowledge transfer

The transfer of tacit knowledge, one of the most important issues in the knowledge sharing context, needs a multi-dimensional perception in its process. Information technology's (IT) supporting role has already been addressed in the process of tacit knowledge transfer. However, IT has its own characteristics, and in turn, may have dissimilar support suitability. On the basis of the knowledge transfer process proposed by Garavelli et al. (Garavelli, C., Gorgoglione, M., Scozzi, B., 2002. Managing knowledge transfer by knowledge technologies. Technovation 22, 269–279), this study conducts an assessment using the analytic hierarchy process (AHP) technique to disclose (1) importance rank of transfer stages for the transfer efficacy and (2) the support suitability of IT types for the transfer stages. The assessment hierarchy has three levels, which are the goal, process and support levels. According to the 21 domain scholars and specialists' assessment analysis, the main results suggest that (1) for goal level, both the knowledge provider's and receiver's cognitive system are of more importance, (2) for process level, database techniques and information system applications obtain the higher importance in supporting the provider's cognitive system and externalisation, and (3) software tools and information system applications are more likely to support the receiver's cognitive system and interpretation. Discussion and implications are also addressed.

Phylogeographic patterns and possible incipient domestication of Jacaratia mexicana A. DC. (Caricaceae) in Mexico

Jacaratia mexicana A. DC. (Caricaceae) is a tropical tree distributed throughout Mexico and Mesoamerica. Some evidence in Mexico indicates the presence of an incipient domestication process in this species. Phylogeographical analyses can potentially determine contemporary patterns of gene flow, isolation between population lineages, as well as historical processes such as population bottlenecks or expansions on their geographical areas. In this study we reconstruct the phylogeographical patterns in populations of J. mexicana A. DC., in order to find differences between genetic variation among wild and cultivated populations utilizing chloroplast DNA and nuclear DNA sequences. We generate a Bayesian phylogenetic tree, to estimate the divergence time between clades using calibrated mutation rates. We also infer the demographic history of these populations using neutrality tests among wild and cultivated accessions. We identified higher levels of haplotype and nucleotide diversity for the cpDNA and ITS types in wild populations than in domesticated populations. These results indicate a reduction of genetic diversity derived from human selection on domestication traits. Neutrality test suggests population expansion detected by the significant negative values of Fu’s Fs in the cultivated populations of this specie. These process results in an excess of rare polymorphism with the fixation of certain advantageous mutation throughout time, this implication are in accordance with the role of the strong selection in the fruit traits of J. mexicana. The dated phylogeny constructed with BEAST program indicated a dispersion pattern for the J. mexicana ancestors across the South Pacific and South Eastern populations during the late Pliocene. Posterior dispersion and divergence in the clades from Central Mexico and North Pacific are in agreement with the episodes of mountain-building in different regions of Mexico.