Isoluminant Stimuli Research Articles

Perception at isoluminance: Role of spatial resolution and background colour.

Introduction. The isoluminance paradigm, in which chromatically defined stimuli are matched for luminance, was developed to investigate the role of colour information in various aspects of visual processing. Performance is typically impaired, leading to the conclusion that particular tasks, e.g. motion and stereo processing, are substantially colour blind. However, other techniques that do not use isoluminance have shown that these processes are strongly sensitive to colour. One such technique is the chromatic noise-in-luminance approach: stimuli are defined by both colour and luminance, but the stimuli to be matched have opposite luminance polarities, e.g. a chromatic dot changes its luminance polarity as is moves. This leads to the question: why are some visual tasks impaired when isoluminant stimuli are used, even though the system is sensitive to colour information? Here we tested two possibilities: 1) colour sensitive cells have poor spatial resolution, and hence are impaired when fine spatial-resolution is required; 2) impairment is specific to isoluminant stimuli presented on a red background (Breitmeyer & Williams, 1990 Vis Res). Methods. Motion and form processing were assessed using global-dot-motion and Glass patterns, respectively. A temporal 2AFC procedure was used. Dot/dipole densities varied from 1 to 2 dots/deg2. The background was either achromatic (grey) or chromatic (red or green). Results. When the grey background was used, performance for the isoluminant dots was not impaired by increasing dot density. Thresholds being at least equal to the chromatic noise-in-luminance condition. Use of a red background impaired performance, particularly with the motion stimuli. Conclusions. Increasing dot density does not impair the processing of all isoluminant stimuli. However, when the stimuli and the background are both chromatic (red and green) performance is impaired, especially with motion stimuli on a red background. Meeting abstract presented at VSS 2014

The effects of luminance contribution from large fields to chromatic visual evoked potentials

Though useful from a clinical and practical standpoint uniform, large-field chromatic stimuli are likely to contain luminance contributions from retinal inhomogeneities. Such contribution can significantly influence psychophysical thresholds. However, the degree to which small luminance artifacts influence the chromatic VEP has been debated. In particular, claims have been made that band-pass tuning observed in chromatic VEPs result from luminance intrusion. However, there has been no direct evidence presented to support these claims. Recently, large-field isoluminant stimuli have been developed to control for intrusion from retinal inhomogeneities with particular regard to the influence of macular pigment. We report here the application of an improved version of these full-field stimuli to directly test the influence of luminance intrusion on the temporal tuning of the chromatic VEP. Our results show that band-pass tuning persists even when isoluminance is achieved throughout the extent of the stimulus. In addition, small amounts of luminance intrusion affect neither the shape of the temporal tuning function nor the major components of the VEP. These results support the conclusion that the chromatic VEP can depart substantially from threshold psychophysics with regard to temporal tuning and that obtaining a low-pass function is not requisite evidence of selective chromatic activation in the VEP.

On using isoluminant stimuli to separate magno- and parvocellular responses in psychophysical experiments—A few words of caution

Isoluminant (or equiluminant) color stimuli (i.e., those that contain variations only in chromaticity) have been employed in attempts to separate magno- and parvocellular responses in psychophysical and noninvasive electrophysiological experiments. The justification for this has been the assumption that magnocellular cells, unlike parvocellular neurons, do not respond to stimuli varying only in hue. However, several problems are associated with this notion: (1) under many conditions, magnocellular neurons are not fully silenced at isoluminance, and (2) in many circumstances, parvocellular responses are substantially reduced at isoluminance. To rely upon isoluminant stimuli to "bias" stimuli toward the parvocellular system also faces obstacles. Therefore, caution is required when attempting to use isoluminant color to separate magno- and parvocellular responses.

Low-level and high-level modulations of fixational saccades and high frequency oscillatory brain activity in a visual object classification task.

Until recently induced gamma-band activity (GBA) was considered a neural marker of cortical object representation. However, induced GBA in the electroencephalogram (EEG) is susceptible to artifacts caused by miniature fixational saccades. Recent studies have demonstrated that fixational saccades also reflect high-level representational processes. Do high-level as opposed to low-level factors influence fixational saccades? What is the effect of these factors on artifact-free GBA? To investigate this, we conducted separate eye tracking and EEG experiments using identical designs. Participants classified line drawings as objects or non-objects. To introduce low-level differences, contours were defined along different directions in cardinal color space: S-cone-isolating, intermediate isoluminant, or a full-color stimulus, the latter containing an additional achromatic component. Prior to the classification task, object discrimination thresholds were measured and stimuli were scaled to matching suprathreshold levels for each participant. In both experiments, behavioral performance was best for full-color stimuli and worst for S-cone isolating stimuli. Saccade rates 200–700 ms after stimulus onset were modulated independently by low and high-level factors, being higher for full-color stimuli than for S-cone isolating stimuli and higher for objects. Low-amplitude evoked GBA and total GBA were observed in very few conditions, showing that paradigms with isoluminant stimuli may not be ideal for eliciting such responses. We conclude that cortical loops involved in the processing of objects are preferentially excited by stimuli that contain achromatic information. Their activation can lead to relatively early exploratory eye movements even for foveally-presented stimuli.

BOLD Responses in Human Primary Visual Cortex are Insensitive to Substantial Changes in Neural Activity

The relationship between blood oxygenation level dependent-functional magnetic resonance imaging (BOLD-fMRI) and magnetoencephalography (MEG) metrics were explored using low-level visual stimuli known to elicit a rich variety of neural responses. Stimuli were either perceptually isoluminant red/green or luminance-modulated black/yellow square-wave gratings with spatial frequencies of 0.5, 3, and 6 cycles per degree. Neural responses were measured with BOLD-fMRI (3-tesla) and whole head MEG. For all stimuli, the BOLD response showed bilateral activation of early visual cortex that was greater in the contralateral hemisphere. There was variation between individuals but weak, or no evidence, of amplitude dependence on either spatial frequency or the presence of luminance contrast. In contrast, beamformer analysis of MEG data showed activation in contralateral early visual cortex and revealed: (i) evoked responses with stimulus-dependent amplitude and latency; (ii) gamma and high-beta oscillations, with spatial frequency dependent peaks at approximately 30 and 50 Hz, but only for luminance-modulated gratings; (iii) The gamma and beta oscillations appeared to show different spatial frequency tuning profiles; (iv) much weaker gamma and beta responses, and at higher oscillation frequencies, for isoluminant compared to luminance-modulated gratings. The results provide further evidence that the relationship between the fMRI-BOLD response and cortical neural activity is complex, with BOLD-fMRI being insensitive to substantial changes in neural activity. All stimuli were clearly visible to participants and so the paucity of gamma oscillations to isoluminant stimuli is inconsistent with theories of their role in conscious visual perception.

Coarse guidance by numerosity in visual search.

In five experiments, we examined whether the number of items can guide visual focal attention. Observers searched for the target area with the largest (or smallest) number of dots (squares in Experiment 4 and "checkerboards" in Experiment 5) among distractor areas with a smaller (or larger) number of dots. Results of Experiments 1 and 2 show that search efficiency is determined by target to distractor dot ratios. In searches where target items contained more dots than did distractor items, ratios over 1.5:1 yielded efficient search. Searches for targets where target items contained fewer dots than distractor items were harder. Here, ratios needed to be lower than 1:2 to yield efficient search. When the areas of the dots and of the squares containing them were fixed, as they were in Experiments 1 and 2, dot density and total dot area increased as dot number increased. Experiment 3 removed the density and area cues by allowing dot size and total dot area to vary. This produced a marked decline in search performance. Efficient search now required ratios of above 3:1 or below 1:3. By using more realistic and isoluminant stimuli, Experiments 4 and 5 show that guidance by numerosity is fragile. As is found with other features that guide focal attention (e.g., color, orientation, size), the numerosity differences that are able to guide attention by bottom-up signals are much coarser than the differences that can be detected in attended stimuli.

A Chromatic Transient Visual Evoked Potential Based Encoding/Decoding Approach for Brain–Computer Interface

This paper presents a new encoding/decoding approach to brain-computer interface (BCI) based on chromatic transient visual evoked potential (CTVEP). The proposed CTVEP-based encoding/decoding approach is designed to provide a safer and more comfortable stimulation method than the conventional VEP-based stimulation methods for BCI without loss of efficiency. For this purpose, low-frequency isoluminant chromatic stimuli are time-encoded to serve as different input commands for BCI control, and the superior comfortableness of the proposed stimulation method is validated by a survey. A combination of diversified signal processing techniques are further employed to decode the information from CTVEP. Based on experimental results, a properly designed configuration of the CTVEP-based stimulation method and a tailored signal processing framework are developed. It is demonstrated that high performance (at information transfer rate: 58.0 bits/min, accuracy: 94.9%, false alarm rate: 1.3%) for BCI can be achieved by means of the CTVEP-based encoding/decoding approach. It turns out that to achieve such good performance, only simple signal processing algorithms with very low computational complexity are required, which makes the method suitable for the development of a practical BCI system. A preliminary prototype of such a system has been implemented with demonstrated applicability.

EEG alpha rhythms and transient chromatic and achromatic pattern visual evoked potentials in children and adults

Transient chromatic pattern visual evoked potentials (VEPs) have been found to be less repeatable in morphology in children than in adults at low to moderate chromatic contrasts. The purpose of this study is to investigate whether low repeatability of VEP components can be associated with high alpha power, in a comparison of alpha activity in children and adults. Transient chromatic contrast and achromatic resolution VEPs were recorded in children (n=14, mean 9.6years) and adults (n=12, mean 21.8years) with normal vision and assessed for repeatability. Isoluminant chromatic (magenta-cyan) and luminance-modulated achromatic grating stimuli were presented at and above psychophysical threshold levels, in pattern onset-offset at 2Hz temporal frequency. EEGs (eyes closed and open) were recorded as single sweeps (1s long) over three 30s periods while facing a uniform computer display. An index of VEP detectability by observation was developed based on VEP component repeatability. The index was examined for correlations with alpha-wave parameters. Alpha power was calculated as the sum of the powers of 8-13Hz frequencies of the EEG sweeps (using the discrete Fourier transform). Alpha power variability was calculated using the standard deviation of the powers of each sweep in a 30s time period. The children had significantly higher alpha powers than the adults for both the eyes-open and eyes-closed conditions. Alpha power variability was significantly higher for the eyes-open condition only. There was no relationship between alpha power parameters and index of VEP detectability by observation for both the chromatic and achromatic grating stimuli. Poor repeatability of transient pattern VEPs is not associated with high alpha power or its variability in EEG measurements in older children or young adults at Oz.

Chromatic Detection in Non-Human Primates: Neurophysiology and Comparison with Human Chromatic Sensitivity

Chromatic detection experiments in humans have been instrumental in elucidating the post-receptoral mechanisms that mediate color vision. To investigate the neural substrates of these mechanisms, we trained Rhesus monkeys to perform a spatial 2AFC chromatic detection task. Our goals were two-fold: First, to assess the utility of Rhesus monkeys as a model for human chromatic detection, and second, to measure the quality of color signals available in cortical area V1 that might subserve detection of isoluminant stimuli. Monkeys proved to be exquisitely sensitive psychophysical observers – matching or exceeding the sensitivity of human subjects performing the detection task under identical stimulus conditions. The sensitivity of humans and monkeys depended similarly on the spatial frequency and chromaticity of the stimulus: sensitivity was low-pass for isoluminant stimuli and bandpass for achromatic stimuli. When stimuli were equated for cone-contrast, sensitivity was greater for L-M modulations than for S-cone modulations. In a subset of these experiments, we recorded from individual V1 neurons in monkeys performing the detection task. Stimuli in these experiments were tailored to each neurons' preferred orientation and spatial frequency. Neuronal and psychophysical sensitivities were compared directly via an ideal observer analysis of firing rates during the stimulus presentation period. Although the sensitivity of individual neurons varied considerably, the most sensitive V1 neurons were roughly as sensitive as the monkey. Accordingly, detection thresholds of V1 neurons varied with color direction in qualitative agreement with the monkey's psychophysical behavior. These data demonstrate the existence of individual V1 neurons that are exquisitely sensitive to chromatic stimuli and attest to the value of Rhesus monkeys as a model for human chromatic detection.

Isoluminant motion onset captures attention

In their 2003 article, Abrams and Christ found that the onset of motion captured attention more effectively than either the offset of motion or continuous motion. Abrams and Christ conceptualized the capture to be occurring at a level higher than does detection of luminance changes in the stimulus. To examine this claim, in the present experiments we replicated their critical experiment but used isoluminant stimuli, which do not produce the low-level luminance transients typically associated with motion. Under isoluminant conditions, we found a pattern of results very similar to that found previously with luminance-defined stimuli, indicating that attention can be prioritized on the basis of perceived motion onset by an object in the absence of low-level luminance transients. This may reflect an evolutionary adaptation to bias attention toward objects that exhibit characteristics of animacy, such as abruptly changing from a static to a dynamic state.

Chromatic Hermann Grid illusions occur with isoluminant stimuli

Chromatic Hermann Grid illusions occur with isoluminant stimuli

Selective attention modulates electrical responses to reversals of optic-flow direction

Attended stimuli typically evoke larger event-related potentials (ERPs) than unattended stimuli. We previously reported an exception when an optic-flow pattern is interleaved with stationary dots. Reversals of motion direction evoked a larger N200 peak when attention was directed to the stationary dots. We replicated and further characterized this result: the N200 enhancement was eliminated when the dots moved randomly rather than in optic flow. The effect was also attenuated with isoluminant stimuli. Electrical source analysis suggested the attentional modulation of a configuration of dorsal extrastriate generators. The ERP evoked by reversals of optic flow may reflect the operation of independently configurable attentional filters within visual cortex.

Grasping isoluminant stimuli

We used a virtual reality setup to let participants grasp discs, which differed in luminance, chromaticity and size. Current theories on perception and action propose a division of labor in the brain into a color proficient perception pathway and a less color-capable action pathway. In this study, we addressed the question whether isoluminant stimuli, which provide only a chromatic but no luminance contrast for action planning, are harder to grasp than stimuli providing luminance contrast or both kinds of contrast. Although we found that grasps of isoluminant stimuli had a slightly steeper slope relating the maximum grip aperture to disc size, all other measures of grip quality were unaffected. Overall, our results do not support the view that isoluminance of stimulus and background impedes the planning of a grasping movement.

Smooth Pursuit Eye Movements to Isoluminant Targets

At slow speeds, chromatic isoluminant stimuli are perceived to move much slower than comparable luminance stimuli. We investigated whether smooth pursuit eye movements to isoluminant stimuli show an analogous slowing. Beside pursuit speed and latency, we studied speed judgments to the same stimuli during fixation and pursuit. Stimuli were either large sine wave gratings or small Gaussians blobs moving horizontally at speeds between 1 and 11 degrees /s. Targets were defined by luminance contrast or color. Confirming prior studies, we found that speed judgments of isoluminant stimuli during fixation showed a substantial slowing when compared with luminance stimuli. A similarly strong and significant effect of isoluminance was found for pursuit initiation: compared with luminance targets of matched contrasts, latencies of pursuit initiation were delayed by 50 ms at all speeds and eye accelerations were reduced for isoluminant targets. A small difference was found between steady-state eye velocities of luminance and isoluminant targets. For comparison, we measured latencies of saccades to luminance and isoluminant stimuli under similar conditions, but the effect of isoluminance was only found for pursuit. Parallel psychophysical experiments revealed that different from speed judgments of moving isoluminant stimuli made during fixation, judgments during pursuit are veridical for the same stimuli at all speeds. Therefore information about target speed seems to be available for pursuit eye movements and speed judgments during pursuit but is degraded for perceptual speed judgments during fixation and for pursuit initiation.

The impulse response of S-cone pathways in detection of increments and decrements.

Impulse response functions (IRFs) were obtained from two-pulse detection thresholds using isoluminant stimuli that produced increments or decrements in S-cone excitation. The pulses were chromatically modulated at constant luminance (based on 18 Hz heterochromatic flicker photometry). Chromatic stimuli were presented as a Gaussian patch (+/-1 SD = 2.3 degrees) in one of four quadrants around a central fixation cross on a CRT screen. Each of the two pulses (6.67 ms) was separated by an inter-stimulus interval (ISI) from 20 to 360 ms. Chromaticity of the pulses was changed from the equal-energy white of the background to a bluish or yellowish color along individually determined tritan lines (based on color matching under strong S-cone adaptation from a 420 nm background superimposed in Maxwellian view). Chromatic detection thresholds were determined by a four-alternative forced-choice method with staircases for each ISI interleaved in each session. Measurements were repeated in at least four sessions for each observer. IRFs were calculated by varying four parameters of an exponentially-damped sinewave. Both S-cone increment and decrement IRFs are characterized by a single excitatory phase and a much longer time course compared with IRFs derived for luminance modulation using the same apparatus and observers. S-cone increment IRFs are faster than S-cone decrement IRFs; the time to peak amplitude of S-cone increment and decrement IRFs is 50-70 and 100-120 ms, respectively. These results were used to derive the temporal contrast sensitivity for human observers of putative ON- and OFF-channels carrying signals from S-cones.

Visual Search: Magno- and Parvocellular Systems or Color and Luminance Processes?

It has been found that visual search is slower with isoluminant color stimuli than with luminance stimuli. This has been interpreted to mean that the magnocellular system plays a vitally important role in visual search. We here propose that this observation can be more parsimoniously understood in terms of color and luminance rather than in terms of the magno-and parvocellular systems. According to this interpretation, the slower search under isoluminant condition is a consequence of the fact that visual processing in general is slower, and that spatial vision is poorer, under isoluminant color conditions relative to luminance conditions. We also point out that in order to draw meaningful conclusions with regard to the underlying neural substrate, one needs to equate the luminance and color stimuli for discriminability.

Constructing isoluminant stimuli for word recognition research: A precautionary study

Isoluminant stimuli are used increasingly often to investigate processes underlying visual word recognition. However, construction of isoluminant stimuli is not straightforward, and inappropriate construction may have the result of misinforming theories that relate word recognition to neurological function. To inform the use of isoluminant stimuli in studies of word recognition, the present article details two experiments in which isoluminant stimuli were constructed using physical onscreen luminance matching and heterochromatic flicker photometry (HFP) with four different stimulus types: disks, squares, rectangles, and letter strings. The findings reveal (1) substantial differences between isoluminance determined by physical onscreen luminance matching and HFP, (2) substantial differences in HFP isoluminance across stimulus types, and (3) substantial differences in HFP isoluminance across participants. These findings indicate that, in contrast to common practice in word recognition research, HFP provides a better indication of isoluminance than physical onscreen matching; but HFP stimuli should match those used in the experiment proper and should be used to assess isoluminance individually for each participant.

Attention, Dyslexia, and the Line-Motion Illusion

Dyslexic readers have been found to have a reduced line-motion illusion. This has been interpreted as evidence for an attention deficit of magnocellular origin. We show that this interpretation has severe problems: 1) to link reduced line-motion illusion to attention overlooks other factors, 2) to link the line-motion illusion specifically to the magnocellular system is problematic because the illusion can be obtained with isoluminant stimuli, 3) reduced illusory motion in dyslexic individuals may reflect sensory deficits, and 4) to link dyslexia specifically to the magnocellular system is in general problematic.

Color scaling of discs and natural objects at different luminance levels

Assigning a basic color name to an object and rating the amount of a particular hue is a fundamental visual capability. Traditional color scaling studies have used increment flashes or isoluminant stimuli of a homogeneous color. Natural objects, however, do not contain a single color but are characterized by a distribution of different chromatic hues. Here we study color scaling using photographs of natural fruit objects. Stimuli were either homogeneous spots, digital photographs of fruit objects (e.g., banana), or outline shapes of the fruit objects. Stimuli were displayed on a CRT monitor on a homogeneous white background; its luminance was varied above and below the medium gray. The chromaticity of the stimuli was varied in 36 equally spaced chromatic directions in the isoluminant plane of the Derrington-Krauskopf-Lennie (DKL) color space. For each stimuli, subjects rated the amount of red, green, blue, and yellow in the stimulus on a scale from 0-8. In agreement with earlier studies we found that the positions of the peak ratings for each color do not coincide with the cardinal axis of DKL color space and are largely invariant under changes of the background luminance. For the average rating we found a dependence on background luminance for all colors: yellow ratings increase with darker backgrounds, whereas ratings for the other colors, in particular green, decrease. For the fruit objects, we found a selective increase in the average color rating for the natural fruit color. For example, the average rating for yellow was 1.7 times higher for the banana images compared to disc stimuli. No such selective increase was found for outline shapes. We conclude that the distribution of hues in natural objects with a characteristic object color can have a profound effect on color scaling and color appearance.

Chromatic VEP assessment of human macular pigment: Comparison with minimum motion and minimum flicker profiles

To assess the effects of macular pigment optical density (MPOD) on isoluminant stimuli and to quantify MPOD electrophysiologically, MPOD distribution profiles were obtained in normal subjects using minimum motion and minimum flicker photometry. Isoluminance of VEP stimuli was determined using minimum flicker and tritan confusion lines were determined using a minimum distinct border criterion. Onset-offset and reversal VEPs to isoluminant red/green, blue/green, and subject-specific tritan gratings of different diameters were recorded from the same 14 subjects tested psychophysically. VEPs were additionally recorded to annular gratings. Chromatic VEP selectivity was assessed by Fourier analysis and as an index; onset negativity/(onset negativity + onset positivity). Peak MPOD varied between 0.2-0.8. Chromatic onset VEPs to all isoluminant 3-deg fields were predominantly negative. Larger blue/green and tritan stimuli elicited VEPs with additional positive, achromatic components; for 9-deg gratings, peak MPOD showed negative correlation with the power of the VEP fundamental (r = -0.70) and with the selectivity index (r = -0.83). Annular gratings elicited chromatic-specific B/G VEPs but only when isoluminance was determined for the annulus. Chromatic selectivity loss in VEPs to large B/G or Tritan gratings can be used to estimate subject-specific MPOD. An important implication is that isoluminant Tritan stimuli with short-wavelength components must be restricted in size in order to optimize koniocellular selectivity.