On using isoluminant stimuli to separate magno- and parvocellular responses in psychophysical experiments—A few words of caution

Abstract

Isoluminant (or equiluminant) color stimuli (i.e., those that contain variations only in chromaticity) have been employed in attempts to separate magno- and parvocellular responses in psychophysical and noninvasive electrophysiological experiments. The justification for this has been the assumption that magnocellular cells, unlike parvocellular neurons, do not respond to stimuli varying only in hue. However, several problems are associated with this notion: (1) under many conditions, magnocellular neurons are not fully silenced at isoluminance, and (2) in many circumstances, parvocellular responses are substantially reduced at isoluminance. To rely upon isoluminant stimuli to "bias" stimuli toward the parvocellular system also faces obstacles. Therefore, caution is required when attempting to use isoluminant color to separate magno- and parvocellular responses.