Iodine-based Disinfectant Research Articles

Application of Closed Incision Negative Pressure Wound Therapy in Ventral Hernia Repair Surgery Using a Polypropylene Mesh: A Randomized Clinical Trial.

Background and Objectives: Surgical site infections (SSIs) are a significant complication following ventral hernia repair, potentially leading to prolonged hospital stays and increased morbidity. This study aimed to evaluate whether closed incision negative pressure wound therapy (ciNPWT) reduces the incidence of SSI after ventral hernia repair with polypropylene mesh compared to standard wound care. Materials and Methods: A randomized study was conducted with 100 patients undergoing ventral hernia repair using a polypropylene mesh. Participants were divided into two groups: a control group (n=50), which received standard sterile gauze dressing with an iodine-based disinfectant, and an intervention group (n=50), treated with the ciNPWT system (Vivano® by HARTMANN) for 5 days postoperatively. The primary outcome was the incidence of SSI within one year after surgery. Secondary outcomes included the influence of factors such as age, sex, smoking status, and hernia size on SSI occurrence. The study was approved by the Ethics Committee at the University Hospital Ostrava, adhering to the ethical standards of the Helsinki Declaration. Results: The incidence of SSI was lower in the ciNPWT group compared to the standard care group (4% vs. 12%), though this difference did not reach statistical significance. No significant effect of sex or smoking status on SSI was observed. The control group had a shorter mean length of hospital stay. Larger hernias in the non-ciNPWT group were more prone to SSIs, as expected. Conclusions: Although limited by a small sample size, the findings suggest that ciNPWT may be associated with a reduced rate of SSI following ventral hernia repair. Further studies with larger populations are needed to confirm these results.

Enhancing Animal Welfare and Immune Health: A Study on Hydrogen Peroxide and Iodine-Based Disinfectants in Farms

Enhancing Animal Welfare and Immune Health: A Study on Hydrogen Peroxide and Iodine-Based Disinfectants in Farms

Disinfectants for Dental Setting in Coronavirus Disease 2019 Era

The introduction of novel variants of infectious viruses causes viral outbreaks. And now we are in the midst of a virus pandemic that is affecting the entire world. The lack of efficient antiviral treatments for novel viral infections, along with the infection's rapid spread in the population, frequently results in significant human and financial damage. Close human-to-human touch or contact with a contaminated surface can both transmit viruses. To prevent viral spread, thorough cleaning or sanitization is required. There are a variety of disinfectants/sanitizing agents/biocidal agents that can inactivate viruses, but their effectiveness is dependent on a number of parameters, including agent concentration, reaction time, temperature, and organic load. In this review, we will be discussing the alcohol- and iodine-based disinfectants in a dental health-care setting.

Study for Formation Root of Off-Flavor Substance 2-Iodo-4-methylphenol in Milk

Food can sometimes contain odors not normally associated with that food. These odors are called off-flavors, and cow milk is known to contain off-flavors such as hexanal depending on the feed or physical condition of the cows. We previously discovered and revealed the structure of 2-Iodo-4-methylphenol (2I4MP), an off-flavor component in commercially available cow milk. We were the first group to discover 2I4MP is an off-flavor component in cow milk.This study investigated the following three questions. First, what compounds serve as the starting materials in generating 2I4MP? Organic synthesis techniques were used to identify p-cresol as a candidate starting material. Second, how is 2I4MP generated in the cowshed? Using cow dung, we confirmed that 2I4MP was generated by the effects of iodine on cow dung. Based on these findings, measures were proposed to prevent contamination of cow milk by 2I4MP. Third, how is 2I4MP transferred to raw milk? 2I4MP movement was estimated by creating a desiccator-based model that showed 2I4MP is potentially transferred to raw milk in the cowshed. These involve that the iodine-based disinfectants, used to disinfect teats during milking, should not be used excessively. Furthermore, ensuring the disinfectants do not fall onto cow dung or bedding. Adopting these measures is vital for preventing contamination of cow milk by 2I4MP.

Factors Driving Bacterial Microbiota of Eggs from Commercial Hatcheries of European Seabass and Gilthead Seabream.

A comprehensive understanding of how bacterial community abundance changes in fishes during their lifecycle and the role of the microbiota on health and production is still lacking. From this perspective, the egg bacterial communities of two commercially farmed species, the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata), from different aquaculture sites were compared, and the potential effect of broodstock water microbiota and disinfectants on the egg microbiota was evaluated. Moreover, 16S ribosomal RNA gene sequencing was used to profile the bacterial communities of the eggs and broodstock water from three commercial hatcheries. Proteobacteria were the most common and dominant phyla across the samples (49.7% on average). Vibrio sp. was the most highly represented genus (7.1%), followed by Glaciecola (4.8%), Pseudoalteromonas (4.4%), and Colwellia (4.2%), in eggs and water across the sites. Routinely used iodine-based disinfectants slightly reduced the eggs’ bacterial load but did not significantly change their composition. Site, species, and type of sample (eggs or water) drove the microbial community structure and influenced microbiome functional profiles. The egg and seawater microbiome composition differed in abundance but shared similar functional profiles. The strong impact of site and species on egg bacterial communities indicates that disease management needs to be site-specific and highlights the need for species- and site-specific optimization of disinfection protocols.

Iodide Residues in Milk Vary between Iodine-Based Teat Disinfectants.

Majority of iodine found in dairy milk comes from the diet and teat disinfection products used during milking process. The objective of this study was to evaluate the effects of 4 iodine-based teat dips on milk iodide concentrations varying in iodine level (0.25% vs. 0.5%, w/w), normal low viscosity dip versus barrier dip, and application method (dip vs. spray) to ensure safe iodine levels in dairy milk when these products are used. The iodine exposure study was performed during a 2-wk period. The trial farm was purged of all iodine-based disinfection products for 21 d during a prestudy "washout period," which resulted in baseline milk iodide range of 145 to 182 ppb. During the experiment, iodine-based teat dips were used as post-milking teat disinfectants and compared to a non-iodine control disinfectant. Milk iodide residue levels for each treatment was evaluated from composited group samples. Introduction of different iodine-based teat disinfectants increased iodide residue content in milk relative to the control by between 8 and 29 μg/L when averaged across the full trial period. However, residues levels for any treatment remained well below the consumable limit of 500 μg/L. The 0.5% iodine disinfectant increased milk iodide levels by 20 μg/L more compared to the 0.25% iodine. Compared to dip-cup application, spray application significantly increased milk iodide residue by 21 μg/L and utilized approximately 23% more teat dip. This carefully controlled study demonstrated an increase in milk iodide concentrations from iodine disinfectants, but increases were small and within acceptable limits.

Virucidal Effect of Commercially Available Disinfectants on Equine Group A Rotavirus

ABSTRACTAlthough many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group A rotavirus (RVA) is limited. We evaluated the performance of commercially available disinfectants against equine RVA. Chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. Glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect was reduced by low temperature or short reaction time, or both. Benzalkonium chloride had the greatest virucidal effect among the three quaternary ammonium compounds examined, but its effect was reduced by the presence of organic matter or by low temperature or a short reaction time. These findings will be useful for preventing the spread of equine RVA infection.

Effect of disinfecting teats post-milking or pre- and post-milking on intramammary infection and somatic cell count

AIMS: To determine the effects of (a) post-milking teat disinfection compared with no disinfection and (b) pre- and post-milking teat disinfection compared with post-milking disinfection alone, on the incidence of new intramammary infection (IMI), somatic cell count (SCC) and teat skin abnormalities in dairy cows. METHODS: In Experiment 1, dairy cows in five dairy herds were randomly allocated to a post-milking teat disinfection group (n=230), that was sprayed with an iodine-based disinfectant (TeatguardPlus) for a complete lactation, or to a non-disinfected group (n=239). In Experiment 2, cows were randomly allocated to post-milking teat disinfection (n=239) or both pre- and post-milking teat disinfection (n=235), using a chloramine-T-based disinfectant (Teatsweet) for both treatments, from calving to 118–127 days in milk. The incidence of new IMI was determined by aseptic sampling of all quarters at calving, during lactation, and at trial end or at drying-off, with clinical mastitis cases sampled on detection. SCC and teat skin abnormalities were measured at 2-monthly intervals during lactation. In both experiments, disinfectant was applied by spray application. RESULTS: Cows that received post-milking teat disinfection had a lower incidence of new IMI caused by Staphylococcus aureus, Streptococcus uberis, Corynebacterium spp and coagulase negative staphylococci, had lower bulk milk SCC during lactation, and had fewer teat skin abnormalities compared with the non-disinfected cows (p < 0.05). Pre-milking teat disinfection, in addition to post-milking teat disinfection, did not reduce the incidence of new IMI for any pathogens and did not reduce SCC (p> 0.05). CONCLUSIONS: Post-milking teat disinfection applied as a spray is a key component in mastitis control in New Zealand. There was no benefit from the addition of pre-milking disinfection. CLINICAL RELEVANCE: This study confirms previous findings of the effectiveness of post-milking teat disinfection in reducing the incidence of IMI caused by the common mastitis-causing pathogens in New Zealand, and presents the first results of a controlled study examining pre-milking teat spraying undertaken in New Zealand commercial dairy herds.

Comparison of Byproduct Formation in Waters Treated with Chlorine and Iodine: Relevance to Point-of-Use Treatment

Due to their efficacy in deactivating a range of microbial pathogens, particularly amoebic cysts, iodine-based disinfectants have been a popular option for point-of-use (POU) drinking water disinfection by campers, the military, and rural consumers in developing countries. Recently, concerns regarding the formation of cytotoxic and genotoxic iodinated disinfection byproducts (I-DBPs) have arisen during chloramine disinfection of iodide-containing waters in the developed world; similar concerns should pertain to iodine-based POU disinfection. Because there are alternative POU disinfection techniques, including chlorine-based disinfectants, this paper compared disinfection byproduct formation from a range of iodine-based disinfectants at their recommended dosages to chlorination and chloramination under overdosing conditions. Just as chloroform was the predominant trihalomethane (THM) forme during chlorination or chloramination, iodoform was the predominant THM formed during iodination. Conditions fostering THM formation were similar between these treatments, except that THM formation during chlorination increased with pH, while it was slightly elevated at circumneutral pH during iodination. Iodoform formation during treatment with iodine tincture was higher than during treatment with iodine tablets. On a molar basis, iodoform formation during treatment with iodine tincture was 20-60% of the formation of chloroform during chlorination, and total organic iodine (TOI) formation was twice that of total organic chlorine (TOCl), despite the 6-fold higher oxidant dose during chlorination. Based upon previous measurements of chronic mammalian cell cytotoxicity for the individual THMs, consumers of two waters treated with iodine tincture would receive the same THM-associated cytotoxic exposure in 4-19 days as a consumer of the same waters treated with a 6-fold higher dose of chlorine over 1 year. Iodoacetic acid, diiodoacetic acid, and other iodo-acids were also formed with iodine tincture treatment, but at levels <11% of iodoform. However, testing of a Lifestraw Personal POU device, which combines an iodinated anion exchange resin with activated carbon post-treatment, indicated minimal formation of I-DBPs and no iodine residual. Although N-nitrosamines have been associated with oxidant contact with anion exchange resins, N-nitrosamine formation rapidly declined to low levels (4 ng/L) using the Lifestraw device after the first few flushes of water.

Use of Murine Norovirus as a Surrogate To Evaluate Resistance of Human Norovirus to Disinfectants

Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correlate with the presence of infectious virus.

Investigation of the iodine–poly(vinylpyrrolidone) interaction employed in the determination of biocidal iodine by colorimetric solid-phase extraction

Colorimetric solid-phase extraction (C-SPE) has been previously explored as a means to monitor the iodine-based disinfectant used in the water systems on board the space shuttle. This same disinfectant is baselined for eventual deployment in the US water recovery system planned for node 3 of the International Space Station (ISS). With C-SPE, the I 2 concentration is determined from the diffuse reflectance spectrum (DRS) of the yellow iodine–poly(vinylpyrrolidone) (PVP) complex using the Kubelka–Munk function. However, the solution chemistry of iodine is very complex and results in a variety of inorganic species (e.g., I −, I 2, I 3 −, HOI) that have very different biocidal capabilities. Thus, the nature of the interaction of iodine with PVP, and more specifically, the identity of the iodine species involved in the interaction, requires more elucidation. This paper reports the findings from a series of detailed experiments conducted to elicit a more complete understanding of the iodine–PVP system employed in C-SPE. The results indicate that I 2, one of the two dominant biocidal forms of iodine, is the species responsible for the analytical signal in our C-SPE platform. These findings lay the ground work for the planned development of a multiplexed iodine determination and speciation platform for in-flight analysis of spacecraft water samples.

Practical intervention strategies for Campylobacter

Campylobacter organisms arc present in the environment of the farm and it is accepted that the chance of infection transferring to chickens is very high. Sources of infection may include any of the standard requirements for poultry such as feed, water and litter. Any form of human intervention as a result of routine animal husbandry requirements may also introduce infection. It has been shown that on some farms it is possible to delay infection by various improvements to bio-security arrangements. The use of dedicated wellington boots for each poultry house and the regular use of foot dips were found to be important factors. The daily use of water sanitiser was also important in delaying the onset of infection. The efficiency of cleaning and disinfection and the construction of the buildings were less significant factors. If flocks were thinned, which involves entry by catching crews and equipment, the risk of infection was dramatically increased. After 42 d of age, the likelihood of infection was also much greater. The effectiveness of these intervention procedures applied in one integrated poultry company are described. Generally, it was felt that even the most stringent bio-security measures applied conscientiously would not be able to prevent infection occurring. Once infection has entered the house, all birds become Campylobacter carriers very quickly. A pen trial was set up to investigate this and the results are described.

Mechanisms of killing of spores of Bacillus subtilis by iodine, glutaraldehyde and nitrous acid.

Treatment of wild-type spores of Bacillus subtilis with glutaraldehyde or an iodine-based disinfectant (Betadine) did not cause detectable mutagenesis, and spores (termed alpha-beta-) lacking the major DNA-protective alpha/beta-type, small, acid-soluble proteins (SASP) exhibited similar sensitivity to these agents. A recA mutation did not sensitize wild-type or alpha-beta- spores to Betadine or glutaraldehyde, nor did spore treatment with these agents result in significant expression of a recA-lacZ fusion when the treated spores germinated. Spore glutaraldehyde sensitivity was increased dramatically by removal of much spore coat protein, but this treatment had no effect on Betadine sensitivity. In contrast, nitrous acid treatment of wild-type and alpha-beta- spores caused significant mutagenesis, with alpha-beta- spores being much more sensitive to this agent. A recA mutation further sensitized both wild-type and alpha-beta- spores to nitrous acid, and there was significant expression of a recA-lacZ fusion when nitrous acid-treated spores germinated. These results indicate that: (a) nitrous acid kills B. subtilis spores at least in part by DNA damage, and alpha/beta-type SASP protect against this DNA damage; (b) killing of spores by glutaraldehyde or Betadine is not due to DNA damage; and (c) the spore coat protects spores against killing by glutaraldehyde but not Betadine. Further analysis also demonstrated that spores treated with nitrous acid still germinated normally, while those treated with glutaraldehyde or Betadine did not.

COMPARISON OF CHLORINE WITH AN IODINE‐BASED COMPOUND ON EGGSHELL SURFACE MICROBIAL POPULATIONS IN A COMMERCIAL EGG WASHER

ABSTRACT Microbial contamination of egg shells is of great importance in the commercial production of table eggs. The objective of this project was to determine the effectiveness of an iodine‐based disinfectant (IBD) on the microbial population of eggshell surfaces under simulated industry egg processing conditions with a commercial egg washer used as the delivery system for sanitizers. Recirculated egg washer water containing 3.69–5.81 log colony‐forming units (CFU)/mL aerobic organisms and 2.02–2.47 g/L total dissolved solids (TDS) was obtained from a commercial egg processing facility and used to simulate conditions found in the commercial egg industry. Sanitizing treatments consisted of distilled deionized water (DDW), IBD, and chlorine (CL; 200 ppm). Enumeration of aerobic plate populations indicated that IBD and CL treatment significantly (p &lt; 0.05) decreased microbial populations on the shell compared to DDW treatment when egg wash water TDS were lower (2.02 – 2.03 g/L) and wash water aerobic plate counts (APC) were higher (5.05 – 5.85 log CFU/mL). When egg wash water TDS was higher (2.47 g/L) and wash water APC were lower (3.69 log CFU/mL) sanitizers were not effective in reducing egg shell microbial populations. No difference in egg shell APC counts was detected between the IBD and CL. In a second trial, cycloheximide or tetracycline amendments were added to media to test the effectiveness of the treatments on either mold and fungi or bacteria alone. When wash water TDS were higher (2.44–2.46 g/L) the sanitizers were again less effective against bacteria compared to samples from lower TDS while fungal populations did not show any significant differences among the treatments. It was concluded from this study that the IBD is an effective sanitizer when used in conjunction with a commercial egg washer but potential efficacy is dependent on the level of TDS in the egg wash water.

Iodine and disinfection: theoretical study on mode of action, efficiency, stability, and analytical aspects in the aqueous system.

Although they have been in use for nearly 170 years, the mode of action of iodine-based disinfectants is not yet clearly understood, as is manifested, for example, in diverging judgements about the relevance of the individual iodine species. Although studies based on calculated equilibrium concentrations in pure iodine solutions have already been done, there is a lack of knowledge about iodine solutions in the presence of additional iodide which would be of intrinsic importance for disinfection practice. Therefore, a re-calculation was undertaken considering variations of this parameter in the pH range 0-14. The presented calculations concern fresh iodine solutions not affected by disproportionation (iodate formation) and provide information about the equilibrium concentrations of the species I, I2, I3, I5-, I6(2-), HOI, O1-, HI2O-, IO2- and H2OI+. Additional iodide and the pH value have a very pronounced influence on the individual equilibrium concentrations (several powers of ten); hence, conditions can be indicated where the number of species of virtual importance is drastically reduced. In the most common case with iodine in the presence of additional iodide at pH < 6, only I-, I2 and I3- play a role. In the absence of additional iodide, at pH 8-9 and at high dilution (c(I2) < 10(-5) M), on the other hand, HOI accounts for over 90% of the oxidation capacity. At high iodide concentration (e.g., Lugol's solution) the species I5- and I6(2-) make up 8.2% of the oxidation capacity. The iodine cation H2OI+, frequently quoted as an active agent in disinfection, is without any relevance under the conditions occurring in practice, as are IO- and HI2O- which become important only at pH > 10. The stability problem (i.e. rate of iodate formation) arising at pH > 6 can be reduced to hypoiodous acid, as manifested in the simple rate law d[IO3]/dt = 0.25 [HOI]3/[H+] which allows an estimation of stability under weakly alkaline conditions. The results of this study allow us to deduce general qualities of aqueous iodine solutions, such as reactivity, stability, and analytical aspects, and to estimate major disinfection-orientated properties such as microbicidal activity, irritation, and incorporation effects. Though the calculations consider primarily preparations devoid of polymeric organic compounds capable of complexing iodine species, the results can be largely transferred to iodophoric preparations.

The uptake and release of molecular iodine by the skin: chemical and bactericidal evidence of residual effects caused by povidone-iodine preparations

The residual effect of iodine-based disinfectants is caused by a dynamic back-diffusion which is the reverse of the absorption occurring during application. A very sensitive photometric method was used to measure the iodine flux of the back-diffusion after treating the skin with povidone-iodine preparations and Lugol's solution. After removal of the preparation the intensity of the iodine flux decreases with time and correlates with the amount of iodine being resorbed. The latter depends on the concentration of free molecular iodine of the preparation, the contact time and the thickness of the horny layer of the treated skin. With Lugol's solution (approximately 170 ppm free molecular iodine) iodine flux could be observed 12–24 h after an application of 20–60 s, while with low-level povidone-iodine preparations (0·5–20 ppm free iodine) the measurable flux lasted only 0·5–1 h after an application time of 3 min. The bactericidal activity of the back-diffusing iodine from skin previously treated with a commercial povidone-iodine preparation was assayed by using an inoculation with Micrococcus luteus. This showed a logarithmic reduction of 0·4 in bacterial concentration. Since the skin was washed with soap for 1 min after application this bactericidal action reflected the residual effect of the back-diffusing iodine.

Some Questions Concerning the Iodine Content in Milk

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