Iodine deficiency has been considered as a serious public health problem for the past decades. Universal salt iodization program is introduced and implemented to address such problem. To encourage this program in an effective and sustainable way, it is essential to regularly monitor whether salt is adequately iodized at various points along the supply chain. The traditional iodometric titration method has problems related to accessibility, cost, and time. Colorimetric test kits have been used extensively to measure coverage of iodized salt in household surveys due to its expediency and affordability. In Thailand, “I-KIT” is the most widely used. The visualization of intensive color, however, is inconvenient for untrained-user in determining the adequacy of iodine content. Thus, an improvement to make testing more precise and affordable is still required. In this respect, a new test kit namely USI-Kit was developed to assess iodine quality and semi-quantity in edible salt. The kit was tested to evaluate its performance, by comparing the result with the I-KIT and with the spectrophotometric method. Compared with I-Kit, the USI-Kit exerted the relative accuracy, sensitivity, specificity, false positive rate, false negative rate and Kappa coefficient value of 74.0, 76.3, 72.6, 27.4, 23.7 and 0.47, respectively. Compared to the spectrophotometric method, USI-Kit exerted the relative accuracy, sensitivity, specificity, false positive rate, false negative rate and Kappa coefficient value of 85.4, 80.1, 89.3, 10.7, 19.9 and 0.70, respectively. The finding suggested that a newly developed iodine test kit holds promise to be used in field inspection of iodine content in salt.