Matrix-assisted laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI) is an analytical technique used for the spatial mapping of drugs, explosives, and organic samples, making it a game-changer in Forensic examination. It detects a wide range of biomolecules in their native state without specific tags, antibodies, labels, and dyes. This review aims to highlight the advancement of MALDI-MSI over time and its impact on Forensic Science due to high-resolution molecular imaging. To foster the development of forensic investigations the utility of MALDI-MSI in six different broad areas, Latent Fingerprinting division,forensic toxicology division,Crime Scene Reconstruction and investigation division, Sex crimes, forensic trichology division, question document analysis, is explored in this review. MALDI-MSI possesses a unique strength of molecular imaging of biomolecules without complex preparation from diverse sample types. In the future, the sensitivity and detection limits of MALDI-MSI can be enhanced and its instrumental size should be reduced to perform on-site investigation.
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