Crop growth and development exhibit high temporal heterogeneity. It is crucial to capture the dynamic characteristics of crop growth using intensive time-series data. However, single satellites are limited by revisit cycles and weather conditions to provide dense time-series data for earth observations. However, up until now, there has been no proposed remote sensing fusion product that offers high spatial-temporal resolution specifically for farmland monitoring. Therefore, focusing on the demands of farmland remote sensing monitoring, identifying quantitative conversion relationships between multiple sensors, and providing high spatial-temporal resolution products is the first step that needs to be addressed. In this study, a fused Landsat 8 (L8) Operational Land Imager (OLI) and Sentinel-2 (S-2) multi-spectral instruments (MSI) data product for regional monitoring of farmland at high, mid, and low latitudes in China is proposed. Two image pairs for each study area covering different years were acquired from simultaneous transits of L8 OLI and S-2 MSI sensors. Then, the isolation forest (iForest) algorithm was employed to remove the anomalous pixels of image pairs and eliminate the influence of anomalous data on the conversion relationships. Subsequently, the adjustment coefficients for multi-source sensors at mixed latitudes with high spatial resolution were obtained using an ordinary least squares regression method. Finally, the L8-S-2 fused dataset based on the adjustment coefficients is proposed, which is suitable for different latitude farming areas in China. The results showed that the iForest algorithm could effectively improve the correlation between the corresponding spectral bands of the two sensors at a spatial resolution of 10 m. After the removal of anomalous pixels, excellent correlation and consistency were obtained in three study areas, and the Pearson correlation coefficients between the corresponding spectral bands almost all exceeded 0.88. Furthermore, we mixed the six image pairs of the three latitudes to obtain the adjustment coefficients derived for integrated L8 and S-2 data with high-spatial-resolution. The significance and accuracy quantification of the adjustment coefficients were thoroughly examined from three dimensions: qualitative and quantitative analyses, and spatial heterogeneity assessment. The obtained results were highly satisfactory, affirming the validity and precision of the adjustment coefficients. Finally, we applied the adjustment coefficients to crop monitoring in three latitudes. The normalized difference vegetation index (NDVI) time-series curves drawn by the integrated dataset could accurately describe the cropping system and capture the intensity changes of crop growth within the high, middle, and low latitudes of China. This study provides valuable insights into enhancing the application of multi-source remote sensing satellite data for long-term, continuous quantitative inversion of surface parameters and is of great significance for crop remote sensing monitoring.