Inversely Related Research Articles

Comparing the Quality of Life of Patients and their Family Members with Dermatological and other Chronic Conditions, in The Bahamas

Background & Objectives: Impact of dermatological and other chronic conditions not only affects the quality of life (QoL) of patients but also that of their family members. This pilot study aimed to compare the QoL impact of dermatological and other chronic conditions on patients with the QoL impact on their family members. Methods: A cross-sectional study using validated QoL questionnaires was conducted. In the dermatological group, patients (>17 years) completed the Dermatology Life Quality Index (DLQI) questionnaire, while children (4-16 years) completed the Children’s Dermatology Life Quality Index (CDLQI) questionnaire. Family members (>18 years) completed both Family Reported Outcome Measure (FROM-16) and Family Dermatology Life Quality Index (FDLQI) questionnaires. In the other chronic conditions group, patients (>17 years) completed the World Health Organization Quality of Life -BREF (WHOQoL-BREF) questionnaire and children (4-17 years) completed The Revised Children’s Quality of Life Questionnaire (KINDL-r: Kiddy KINDL, Kid KINDL and Kiddo KINDL). Family members completed the FROM-16 questionnaire. Data were analysed using IBM SPSS™ statistical software. Results: Forty-four participants completed the study. In the dermatological group (n=26), there was a weak negative correlation between DLQI and FDLQI scores (r= –0.23, p=0.55) not between DLQI and FROM-16 (r = –0.04, p=0.92). There was a very strong positive relationship between both CDLQI and FDLQI (r=0.83, p=0.17) and CDLQI and FROM-16 (r=0.82, p=0.18). Although not statistically significant, there may be a correlation between the FROM-16 and FDLQI scores for family members of dermatology patients. In the chronic conditions group (n=18) the mean score of WHOQoL-BREF was 90.5 (SD=13) with a significant negative inverse relationship to FROM-16 (r= –1.000, p=<0.001). The KINDL-r scores (mean=66, SD =11) showed no significant correlation (r= –0.24, p=0.61) with FROM-16 scores (mean=9.6, SD=3.7). Conclusion: The impact of a patient’s dermatological or other chronic condition can not only negatively affect the patient’s QoL but also the QoL of their family members.

Corporate pollution and reputational exposure

AbstractWe study the empirical association between corporate pollution and reputational exposure using a sample of 745 U.S. firms from 2007 to 2019 and an ordered probit model. Our results reveal an inverse relationship between chemical emissions and reputational exposure rating, after controlling for various firm attributes. We examine the roles of corporate governance structure and the demographic background of the top management team in the transmission process from polluting chemical emissions to reputation. Further, the negative impact of corporate pollution on reputational exposure rating is much stronger in areas where residents are convinced that climate change is happening. We perform several tests and analyses designed to mitigate endogeneity issues and correct sample bias to ensure the robustness of our findings. Finally, our results suggest that the negative effect is stronger for companies with higher information asymmetry, which indicates the importance of information transparency for firms' credibility.

Aqueous Outflow Facility after Periocular Triamcinolone Injection: A Preliminary Evaluation of the Falck Medical Applanation Tonometer.

To evaluate the Falck Medical Applanation Tonometer 1 (FMAT-1) as a device for measuring intraocular pressure (IOP) and outflow facility (OF) in patients with uveitis after periocular triamcinolone injection in one eye. Retrospective observational comparison. IOP and OF measurements were recorded and analyzed, comparing Goldmann tonometry and FMAT-1 readings. Records were reviewed for adult patients with uveitis who had undergone recent periocular triamcinolone injection in one eye. A total of 19 patients' records were evaluated. In treated eyes, median IOPs (in mm Hg) by Goldmann and FMAT-1 were 21 and 21.0, respectively, and mean IOPs were 22.21 and 21.15, respectively. In untreated eyes, median IOPs by Goldmann and FMAT-1 were 15 and 15.7, respectively, and mean IOPs were 15.52 and 15.31, respectively. Median OF (in µL/mm Hg) in treated and untreated eyes was 0.17 and 0.22, respectively, and mean values were 0.17 and 0.23, respectively. Pearson correlation showed an inverse relationship between IOP and OF in treated eyes, and linear regression analysis showed that IOP strongly predicted outflow in this group. The FMAT-1 generates IOP results substantially similar to Goldmann and OF readings with greater ease than prior tonography devices. The mechanism of corticosteroid-induced IOP elevation appears to be a reduction in aqueous OF, and its measurement is tonography. This technique has traditionally been cumbersome. FMAT-1 is a newly launched instrument that measures IOP and OF at the slit lamp simultaneously in a few seconds. Staso FD, Gaudio PA. Aqueous Outflow Facility after Periocular Triamcinolone Injection: A Preliminary Evaluation of the Falck Medical Applanation Tonometer. J Curr Glaucoma Pract 2023;17(4):197-204.

Inverse relationship between platelet Akt activity and hippocampal atrophy: A pilot case-control study in patients with diabetes mellitus.

Akt plays diverse roles in humans. It is involved in the pathogenesis of type 2 diabetes mellitus (T2DM), which is caused by insulin resistance. Akt also plays a vital role in human platelet activation. Furthermore, the hippocampus is closely associated with memory and learning, and a decrease in hippocampal volume is reportedly associated with an insulin-resistant phenotype in T2DM patients without dementia. To investigate the relationship between Akt phosphorylation in unstimulated platelets and the hippocampal volume in T2DM patients. Platelet-rich plasma (PRP) was prepared from the venous blood of patients with T2DM or age-matched controls. The pellet lysate of the centrifuged PRP was subjected to western blotting to analyse the phosphorylation of Akt, p38 mitogen-activated protein (MAP) kinase and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Phosphorylation levels were quantified by densitometric analysis. Hippocampal volume was analysed using a voxel-based specific regional analysis system for Alzheimer's disease on magnetic resonance imaging, which proposes the Z-score as a parameter that reflects hippocampal volume. The levels of phosphorylated Akt corrected with phosphorylated p38 MAP kinase were inversely correlated with the Z-scores in the T2DM subjects, whereas the levels of phosphorylated Akt corrected with GAPDH were not. However, this relationship was not observed in the control patients. These results suggest that an inverse relationship may exist between platelet Akt activation and hippocampal atrophy in T2DM patients. Our findings provide insight into the molecular mechanisms underlying T2DM hippocampal atrophy.

Impact of dynamic working capital management on operational efficiency: empirical evidence from Scandinavia

PurposeConsumer goods firms often tie up inventory and accounts receivable resources, creating cost and liquidity issues. Dynamic working capital management (DWCM) can mitigate these concerns and enhance operational profitability. The study investigates DWCM's impact on operational efficiency (OE).Design/methodology/approachThe empirical estimation uses pooled ordinary least squares (OLS), random effect and system generalized method moments (GMM) regression analysis of consumer goods firms in Scandinavia from 2005 to 2022 to present the results.FindingsThe findings indicate that DWCM has an inverse relationship with operating cost, while positively impacting operating profit. The final outcome demonstrates that DWCM enhances OE. Furthermore, the working capital ratio (WCR) consistently exceeds the cash conversion cycle (CCC) in all models, indicating that prudent management of cash in accounts receivable, inventory and accounts payable leads to higher cost savings and superior performance.Practical implicationsThe results suggest that organizations that prioritize the management of the absolute cash committed to inventory, receivables and payables as much as the CCC experience improved OE.Originality/valueThis paper adds to the literature on how DWCM affects OE in the consumer goods sector. It also highlights the impact of time management and cash management in WCM on OE. Additionally, it analyzes how DWCM variables affect operating costs and profits, shedding light on their efficiency impact.

TPIV Experimental Investigation of Film Coolant-to-Mainstream Interaction From Shaped Cooling Holes With Various Inlet Geometries

Abstract In this investigation, multiple sets of time-averaged tomographic particle imaging velocimetry measurements are completed for laid-back, fan-shaped film cooling holes with “racetrack” shaped inlets. Traditional 10-10-10, laid-back, fan-shaped holes are inclined 30 deg to the mainstream flow on a flat plate. The inlet cross section varies from round to two elongated racetrack shapes. The cross-sectional area and the outlet-to-inlet area ratio for all the geometries are held constant. The flat plate is installed in a low-speed wind tunnel with a mainstream turbulence intensity of 8% and an average velocity of 21.6 m/s. The blowing ratios of the film jets range from 0.6 to 1.5 and the density ratio is 1. The Reynolds number of the cooling jet varies from 2600 to 8400. The characteristics of the resulting flowfield are coupled with the detailed film cooling effectiveness distributions. It can be noted from the results that the counter-rotating vortex pair generated by the 2:1 inlet is the closest to the surface and weakest in strength, likely caused by the minimum peak jet momentum of the three. The Reynolds stresses downstream of the 2:1 and 4:1 inlets are significantly lower than those downstream of the shaped hole with a round inlet. An inverse relation between volumetric turbulence accumulation (TA), and surface effectiveness (η), can be correlated for the blowing ratios considered. The turbulence accumulation term can thus be used to evaluate the performance of a film cooling hole design with flowfield data.

Abstract C077: Elucidation and exploitation of critical nutrient scavenging regulation in ERK MAPK inhibited pancreatic cancer

Abstract Alteration of essential metabolic pathways is a major mechanism by which oncogenic KRAS promotes tumor development and growth in pancreatic ductal adenocarcinoma (PDAC). KRAS-driven PDAC is dependent on nutrient scavenging pathways, including macropinocytosis and autophagy to fuel the high metabolic demand of rapid proliferation. Thus, these metabolic processes are attractive targets for the development of treatments for PDAC. KRAS loss results in downregulation of macropinocytosis in PDAC. Additionally, our lab demonstrated that KRAS loss or inhibition of ERK MAPK signaling decreased glucose consumption and glycolysis but increased autophagy, thereby enhancing dependency on autophagy for survival and growth. Accordingly, dual ERK MAPK and autophagy inhibition (via chloroquine) synergistically enhanced anti-tumor efficacy in PDAC. Early clinical data has demonstrated that resistance to this treatment arises over time through unknown mechanisms. Our preliminary data indicates that following ERK MAPK inhibition both autophagy induction and macropinocytosis downregulation is transient—with autophagic and macropinocytic activity returning to/surpassing basal levels after prolonged treatment. The underlying mechanistic and signaling crosstalk between autophagy and macropinocytosis remains poorly understood. We hypothesize that there is compensatory regulation between autophagy and macropinocytosis signaling following ERK MAPK inhibition. We propose that prolonged activation of autophagy upregulates macropinocytosis over time, consequently abrogating dependency on autophagy and therefore reducing sensitivity to autophagy inhibition. We demonstrate an inverse temporal relationship between autophagic flux and macropinocytosis following genetic loss of KRAS or pharmacological inhibition of the KRAS-ERK MAPK pathway. Furthermore, we show that chloroquine, which is commonly thought of as a lysosomotropic drug, does not prevent degradation of proteins engulfed via macropinocytosis. Together our data suggest that upregulation of macropinocytosis may mediate resistance to the combination of ERK MAPK inhibition and chloroquine. Additionally, we present the unexpected observation that prolonged pharmacological inhibition of the ERK MAPK pathway results in the upregulation of macropinocytosis. Furthermore, we demonstrate that this upregulation of macropinocytosis is accompanied by increased albumin uptake and sensitivity to albumin-bound paclitaxel (nab-paclitaxel). Given the dependence of PDAC on macropinocytic uptake for tumorigenic growth, as well as the potential to exploit macropinocytosis for drug-delivery, it is imperative to understand how ERK MAPK inhibitors regulate macropinocytosis. A better understanding of the regulation underlying these essential metabolic pathways will better inform future ERK MAPK inhibitor combinations. Citation Format: Ryan Robb, Kirsten L. Bryant. Elucidation and exploitation of critical nutrient scavenging regulation in ERK MAPK inhibited pancreatic cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr C077.

Intergranular Corrosion Analysis of Austenitic Stainless Steels in Molten Nitrate Salt Using Electrochemical Characterization

This study investigates the influence of molten nitrate salt exposure on the intergranular corrosion (IGC) behavior of three grades of austenitic stainless steel (namely, AISI 304, AISI 304H, and AISI 321H). Two electrochemical techniques, double loop electrochemical potentiokinetic reactivation and potentiodynamic polarization methods, are applied after stainless steel is exposed to 600 °C molten nitrate salt, 60% NaNO3, and 40% KNO3 for varying immersion durations. Corrosion morphology is examined using optical microscopy and scanning electron microscopy images to assess susceptibility to IGC. IGC is prompted by the presence of chromium carbides at grain boundaries, which leads to chromium depletion around these carbides. The findings of the experiments reveal distinct IGC behavior among stainless steel grades. For AISI 304, the degree of sensitization (DOS) increases as exposure time progresses. However, AISI 304H and AISI 321H stainless steel exhibit diminishing DOS after 100 and 10 h of exposure, respectively. This trend is attributed to desensitization or the healing effect when stainless steel is exposed to molten salt for a prolonged time. The depletion and recovery of Cr near grain boundaries are confirmed by the inverse relationship to DOS of pitting potential.

Abstract A068: Proteomic analysis of keratin 17 positive cells from laser-capture microdissected pancreatic ductal adenocarcinoma

Abstract Introduction. Although patients with pancreatic ductal adenocarcinoma (PDAC) have one of the lowest survival of all carcinomas, there is considerable variation in survival and disease progression, even when accounting for factors such as age and pathologic stage. Our group identified keratin 17 (K17) as a negative prognostic and predictive biomarker in PDAC. Patients with high K17 expression in their tumors have shorter overall survival as well as decreased response to chemotherapeutic agents, including gemcitabine. K17 has also emerged as an established biomarker of the basal molecular subtype of PDAC. In this study, we aim to identify proteins which correlate, directly or indirectly, with K17 expression via a combined approach using immunohistochemistry (IHC) followed by laser capture microdissection (LCM) and mass spectrometry. Methods. We selected 5 PDAC cases of low K17 expression and 5 PDAC cases of high K17 expression from the archival collections from the Stony Brook Cancer Center Biobank. A modified indirect immunoperoxidase approach was performed on sections mounted on LCM-compatible membrane slides. Carcinoma cells that were either K17 positive or negative by IHC were precisely dissected using LMD7 microscope (Leica) from 10-µM thick sections, followed by a modified tissue digestion and protein extraction protocol. Collision-induced fragment mass information and peptide abundance values were obtained by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS). Spectral cutoffs were analyzed at both <5% and 1% false discovery rates. The human UniProt dataset was used for data alignment. Results. Mass spectrometry yielded over 1600 detected proteins with approximately 50 of which correlated strongly with K17 expression. Our preliminary results show that keratin 5, a known marker of basal-like PDAC, is positively correlated with keratin 17. Genes whose increased expression contribute to the Moffitt classification of basal molecular subtype of PDAC, such as S100A2, keratin 6A and keratin 7, also showed positive correlation with K17 in our study. The remainder of proteins associated with K17 expression include those involved with cell adhesion, cell-extracellular matrix interaction and smooth muscle contraction pathways, with specific types and classes being identified, pending in-depth analysis. Proteins with inverse relationship to K17 expression included those involved in intracellular organelle trafficking and apoptosis pathways. Conclusion. Our findings demonstrate the utility of mass spectrometry-based spatial proteomic analysis of microdissected cell populations from formalin-fixed, paraffin embedded samples. These methods have the potential to uncover complementary biomarkers to K17 that could enhance prognostic accuracy or serve as therapeutic targets and provide mechanistic insight into the mechanisms through which K17 drives tumor aggression. Citation Format: Ji Dong K. Bai, John D. Haley, Natalia Marchenko, Luisa F. Escobar-Hoyos, Kenneth R. Shroyer. Proteomic analysis of keratin 17 positive cells from laser-capture microdissected pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr A068.

The value of right ventricular to pulmonary arterial coupling in the critically ill: a National Echocardiography Database of Australia (NEDA) substudy.

Right ventricular (RV) function is tightly coupled to afterload, yet echocardiographic indices of RV function are frequently assessed in isolation. Normalizing RV function for afterload (RV-PA coupling) using a simplified ratio of tricuspid annular plane systolic excursion (TAPSE)/ tricuspid regurgitant velocity (TRV) could help to identify RV decompensation and improve risk stratification in critically ill patients. This is the first study to explore the distribution of TAPSE/TRV ratio and its prognostic relevance in a large general critical care cohort. We undertook retrospective analysis of echocardiographic, clinical, and mortality data of intensive care unit (ICU) patients between January 2012 and May 2017. A total of 1077 patients were included and stratified into tertile groups based on TAPSE/TRV ratio: low (< 5.9mm.(m/s)-1), middle (≥ 5.9-8.02mm.(m/s)-1), and high (≥ 8.03mm.(m/s)-1). The distribution of the TAPSE/TRV ratio across ventricular function subtypes of normal, isolated left ventricular (LV), isolated RV, and biventricular dysfunction was explored. The overall prognostic relevance of the TAPSE/TRV ratio was tested, including distribution across septic, cardiovascular, respiratory, and neurological subgroups. Higher proportions of ventricular dysfunctions were seen in low TAPSE/TRV tertiles. TAPSE/TRV ratio is impacted by LV systolic function but to a lesser extent than RV dysfunction or biventricular dysfunction. There was a strong inverse relationship between TAPSE/TRV ratio and survival. After multivariate analysis, higher TAPSE/TRV ratios (indicating better RV-PA coupling) were independently associated with lower risk of death in ICU (HR 0.927 [0.872-0.985], p < 0.05). Kaplan-Meier analysis demonstrated higher overall survival in middle and high tertiles compared to low tertiles (log rank p < 0.0001). The prognostic relevance of TAPSE/TRV ratio was strongest in respiratory and sepsis subgroups. Patients with TAPSE/TRV < 5.9mm(m/s)-1 had a significantly worse prognosis than those with higher TAPSE/TRV ratios. The TAPSE/TRV ratio has prognostic relevance in critically ill patients. The prognostic power may be stronger in respiratory and septic subgroups. Larger prospective studies are needed to investigate the role of TAPSE/TRV in pre-specified subgroups including its role in clinical decision-making.

Correlation Between a Deficiency of D3 Levels and the Development of Allergic Rhinitis.

&lt;b&gt;Background and Objective:&lt;/b&gt; Allergic rhinitis (AR) is a common disorder characterized by sneezing, runny nose, nasal congestion and lacrimation, which negatively affects the quality of life to a large extent. The study aimed to find a link between the effect of vitamin D3 levels on Immunoglobulin (IgE) levels in patients with allergic AR. &lt;b&gt;Materials and Methods:&lt;/b&gt; This study included 30 patients with AR, with ages ranging from 18 to 35, of both sexes. For vitamin D levels, &lt;u&gt;>&lt;/u&gt;30 ng/mL is considered sufficient and &lt;u&gt;<&lt;/u&gt;20 ng/mL is a deficiency. The second group includes 30 people with adequate levels of vitamin D3 as a control group. All results were analyzed statistically by ANOVA, in addition to using the regression coefficient test to test the extent of the effect of D3 on the development of allergic rhinitis at a significant level of p&lt;u&gt;<&lt;/u&gt;0.05 using the SPSS program 24. &lt;b&gt;Results:&lt;/b&gt; The results showed a significant decrease in the levels of vitamin D3 in the serum of the AR patients compared with the control group and a substantial increase in the levels of IgE in the serum of the AR patients compared with the control group at a significant level of p&lt;u&gt;<&lt;/u&gt;0.05. Additionally, the results showed in the regression coefficient an inverse and significant effect of vitamin D3 concentration on serum IgE levels, which is significant in terms of the p-value, which appeared equal to 0.010. By observing the value of the R&lt;sup&gt;2&lt;/sup&gt; coefficient of determination, it is clear that a change in the concentration of vitamin D3 causes 58% of the changes in IgE levels. &lt;b&gt;Conclusion:&lt;/b&gt; Through linear regression correlation, an inverse linear relationship emerged linking low vitamin D3 levels to increased IgE levels with an effect rate of 58%.

The Role of Renewable Energy Consumption in Targeting Debt Sustainability in African and MENA Region Countries

The primary aim of this study was to explore the impact of renewable energy consumption on debt sustainability in African and Middle East and North African (MENA) countries. Other objectives were to be investigated including foreign direct investment net inflows’ effects on those countries’ public debt, besides the relationship between carbon dioxide emissions and their public debt. The Fixed Effects Regression Model for Panel data analysis was employed through the utilization of different variables data of 21 countries in Africa and MENA regions, covering the period from 1999 to 2021. The conducted regression analysis incorporated central government debt as a dependent variable, and carbon dioxide emissions, FDI net inflows, the government effectiveness index, credit to the private sector, and renewable energy consumption as explanatory variables. The results revealed the existence of direct significant relationship between renewable energy consumption and central government debt, suggesting that reducing public debt leads to lower utilization of renewable energy. This could imply that going for the objective of debt sustainability requires the prevention of the wide utilization of renewable and clean energy. However, this impact could be overcome or lessened or even mitigated if it is aligned with the presence of highly effective governments and stimulated by FDINI oriented towards climate change mitigation since Green foreign direct investment inflows promotes the sustainable development and debt sustainability specifically with the presence of inverse relationship between the latest variables and central government debt. One recommendation from this research is that attracting FDI that promotes sustainable development within the context of effective governments can reduce government debt and should be explored for attaining the main objective without harming the ecological system or causing environmental degradation.

Oxygen increase and the pacing of early animal evolution

While it is widely accepted that early animals originated and primarily evolved during the Neoproterozoic to Cambrian period, there remains ongoing debate over how fluctuations in marine-atmospheric oxygen levels influenced their evolution and diversification. To investigate this, we analyzed pristane/phytane ratios—a redox-proxy based on organic geochemistry—in surface sediments from the Cryogenian to Cambrian successions in South China, Oman, and Australia. The temporal changes in this proxy exhibited consistent patterns across all sites, revealing five cycles of anoxic to oxic conditions in the ocean between 660 and 510 Ma. By examining the average pristane/phytane ratios, we identified three events when oxygen levels increased. This represents a transition from anoxic conditions to anoxic-dysoxic boundary at 630–600 Ma, followed by a shift to dysoxic conditions at 570 Ma (Shuram event), and finally reaching oxic conditions at 520 Ma (Cambrian explosion). Significantly, these oxygenation events align with eumetazoan evolution. An inverse relationship was observed between oceanic redox events and positive/negative shifts of δ13Ccarb, occurring between the first and second oxygenation events, suggesting an increase in atmospheric oxygen levels. Based on these findings, we propose that there were global increases in oceanic and atmospheric oxygen levels, at least during the early Ediacaran period. These shifts in oxygen levels likely played a role in influencing the rate of evolution among early animals.

Computational drug repurposing for primary hyperparathyroidism

In hyperparathyroidism (hyperPTH), excessive amounts of PTH are secreted, interfering with calcium regulation in the body. Several drugs can control the disease's side effects, but none of them is an alternative treatment to surgery. Therefore, new drug candidates are necessary. In this study, three computationally repositioned drugs, DG 041, IMD 0354, and cucurbitacin I, are evaluated in an in vitro model of hyperPTH. First, we integrated publicly available transcriptomics datasets to propose drug candidates. Using 3D spheroids derived from a single primary hyperPTH patient, we assessed their in vitro efficacy. None of the proposed drugs affected the viability of healthy cell control (HEK293) or overactive parathyroid cells at the level of toxicity. This behavior was attributed to the non-cancerous nature of the parathyroid cells, establishing the hyperPTH disease model. Cucurbitacin I and IMD 0354 exhibited a slight inverse relationship between increased drug concentrations and cell viability, whereas DG 041 increased viability. Based on these results, further studies are needed on the mechanism of action of the repurposed drugs, including determining the effects of these drugs on cellular PTH synthesis and secretion and on the metabolic pathways that regulate PTH secretion.

Comprehensive analysis of quality and bioactive compounds in umbu-cajá fruit during different ripening stages

Umbu-cajá (Spondias tuberosa x S. mombin) is a tropical fruit with potential health benefits due to its bioactive compounds. The objective of this study was to identify the optimal harvest point for enhanced nutritional value and consumer appeal of umbu-cajá. Umbu-cajá fruit was collected at five ripening stages (S1 to S5). Immature fruit (S1) had a higher pulp yield but a lower seed yield, suggesting an inverse relationship between these fruit components. Stage 3 (S3) was identified as the optimal harvest time, as it maintained stable pulp and seed mass, corresponding to the ideal physiological maturity point for human consumption. The respiration rate increased significantly at stages S4 and S5, indicating that Spondias sp. could be classified as a climacteric fruit. Color analysis revealed increased yellowness during ripening, which is indicative of the development of desirable attributes such as sweetness and soft texture in hipened fruits. Compositional analysis demonstrated that moisture content decreased while ash and solids content increased during ripening, reflecting appropriate maturity for human consumption. The ratio of soluble solids to acidity increased, suggesting enhanced sweetness as the fruits ripened. The levels of antioxidants, except for anthocyanins, decreased during ripening, with the latter increasing up to stage S3, thereby providing the fruit with its vibrant colors. The compositional changes underscore the importance of considering both nutritional and sensory attributes in determining the optimal harvest time. Moreover, the increased antioxidant content at early ripening stages signifies the fruit's potential as a valuable source of health-promoting compounds. This study contributes to optimizing the quality of umbu-cajá and offers valuable information for sustainable production and improved consumption of these fruits. By identifying the ideal harvest time and evaluating the changes in bioactive compounds and quality attributes during ripening, this research aids in promoting healthier dietary choices and supporting the fruit industry. The findings have implications for producers, retailers, and consumers, providing a scientific basis for enhancing the nutritional value and flavor profile of umbu-cajá at the optimal stage of ripening.

Mitochondrial DNA copy number plays opposing roles in T-lymphocyte infiltration of colorectal cancer based on mismatch repair status: new directions for immunotherapy?

Researchers have previously reported that mitochondrial DNA copy number (mtDNA-CN) can play different roles in microsatellite instable/mismatch repair-deficient (MSI/dMMR) and microsatellite stable/mismatch repair-proficient (MSS/pMMR) colorectal cancer (CRC). To support malignancy, dMMR CRC relies on glycolysis, while pMMR CRC favors oxidative phosphorylation. However, it is unclear whether mtDNA-CN changes are related to T cell infiltration in CRC. The mtDNA-CN was detected by qRT-PCR in 532 patients, and the expression of CD3 and CD8 in 485 patients was detected by immunohistochemistry. The correlation between mtDNA-CN and the prognosis of CRC patients was further analyzed, and the correlation between mtDNA-CN and T lymphocyte infiltration was also analyzed. Biopsy specimens from the immune checkpoint inhibitors (ICIs) treatment cohort were obtained to verify the correlation between mtDNA-CN and the efficacy of ICIs. The effects of mtDNA-CN and MMR status on gene expression were analyzed by RNA-seq. Our results show that mtDNA-CN has inverse relationships to CRC prognosis in cases with different MMR statuses, potentially inducing the U-shaped association in CRC. The opposing correlations between mtDNA-CN and T lymphocyte infiltration in cases of dMMR CRC and pMMR CRC further suggest that mtDNA-CN might play an important role in CRC development. More importantly, cases of pMMR CRC with lower mtDNA-CN and of dMMR CRC with higher mtDNA-CN can benefit more dramatically from ICIs. Furthermore, RNA-seq revealed a link between the level of mtDNA-CN and T lymphocyte infiltration in CRC cases with different MMR statuses. Our study found a potential relationship between mtDNA-CN and CRC development that differs by MMR status, potentially providing a rationale for the use of mtDNA-CN as both a predictive biomarker and a therapeutic target for ICIs.

Numerical analysis and optimization of CFRP buckle arrestors in subsea pipelines

This research investigates the collapse mechanisms and crossover pressure capacity of carbon fiber-reinforced polymer (CFRP) buckle arrestors in subsea pipelines. A nonlinear three-dimensional finite element (FE) model was formulated using ANSYS framework, incorporating a nonlinear geometric algorithm to simulate the collapse and crossover failures. The study underscored a good agreement between the FE model predictions, experimental observations done by the author, and previous research outcomes. Two principal crossover modes; flattening and U-shape modes were identified, though initiated similarly, differ in progression, attributed to the arrestor's attributes. The arrestor thickness ratio (h/t) emerged as a significant determinant of arresting efficiency, particularly impactful when h/t ≥ 1.5. An inverse relationship between σyp/σuc and PX/PP was identified. Furthermore, specific geometric parameters, like h/t and L/D, delineate the predominant crossover mode. Finally, new empirical expressions including the geometric and material parameters are proposed to predict the crossover pressure (PX) for each crossover mode, separately. Also, the findings suggest that CFRP arrestors, especially those with configurations of L = 1.5D and h = 3t, can offer optimum resilience to external pressure.

Environmental Assessment of Green Areas in the City of Campinas, SP: A Study of Sustainability and Biodiversity

Objective: To present a diagnosis of the environmental quality of three (3) urban green areas in Campinas, SP, so that the impact caused by urban pressure arising from the growth of cities can be evaluated. Theoretical Benchmark: Research has been conducted on sustainable development goals, smart cities and urban green areas, and can understand the importance of urban green areas in the sustainable development of smart cities. Method: Application of the LAP (Landscape Assessment Protocol) methodology, where, through Landscape Assessment using 15 metrics, the LAP CI index is obtained. Results and conclusion: The areas evaluated have LAP CI indices ranging from moderate with index 61 for the Taquaral Lagoon, 81 being good for the German Forest and 95, excellent for the Santa Geneva Forest. The methodology used can be applied to different types of areas, reflecting their environmental condition through the urban pressure suffered and having an inverse relationship between the number of visitors to the parks and the index found. Implications of the research: The research demonstrated that the methodology can be applied by different evaluators bringing different views about the environmental quality of urban green areas, precisely for the subjetive character of the evaluator in scoring the metrics. This environmental classification can help decision-makers create urban green area recovery programs. Originality / value: The research showed that the evaluation of urban green areas is an important ally in the search for sustainability in cities in order to provide better quality of life to the population.

Quantifying the Impact of Urban Growth on Urban Surface Heat Islands in the Bangkok Metropolitan Region, Thailand

The urban built environment, comprising structures, roads, and various facilities, plays a key role in the formation of urban heat islands, which inflict considerable damage upon human society. This phenomenon is particularly pronounced in urban areas characterized by the rapid growth and concentration of populations, a global trend, notably exemplified in megacities such as Bangkok, Thailand. The global trend of urbanization has witnessed unprecedented growth in recent decades, with cities transforming into megametropolises that profoundly impact changes in urban temperature, specifically the urban heat island (UHI) phenomenon induced by the rapid growth of urban areas. Elevated urban concentrations lead to increased city density, contributing to higher temperatures within the urban environment compared to the surrounding areas. The evolving land-use surface has assumed heightened significance due to urban development, necessitating accelerated efforts to mitigate urban heat islands. This study aims to quantify the influence of urban growth on urban surface temperature in Bangkok and its surrounding areas. The inverse relationship between urban temperature and land surface temperature (LST), coupled with urban area density, was examined using Landsat 5 and 8 satellite imagery. The analysis revealed a positive correlation between higher temperatures and levels of urban growth. Areas characterized by high-rise structures and economic activities experienced the most pronounced impact of the heat island phenomenon. The city exhibited a notable correlation between high density and high temperatures (high–high), signifying that increased density contributes to elevated temperatures due to heat dissipation (significant correlation of R2 = 0.8582). Conversely, low-temperature, low-density cities (low–low) with a dispersed layout demonstrated effective cooling of the surrounding area, resulting in a significant correlation with lower local temperatures (R2 = 0.7404). These findings provide valuable insights to assist governments and related agencies in expediting planning and policy development aimed at reducing heat in urban areas and steering sustainable urban development.

The Impact of Intangible Capital on Firm Profitability in the Technology and Healthcare Sectors

The aim of the present study is to assess the impact of structural capital intensity and utilization on firm profitability in an international setting: the European Union countries, plus Norway, Switzerland and the United Kingdom. The indicators are calculated based on financial data downloaded from the Refinitiv Eikon database. Two financial ratios are used as proxies for the intensity and utilization of structural capital. The balanced panel consists of 625 companies from 25 countries, over the period from 2013 to 2022. The panel includes financial information on two industries that are considered innovation-oriented, namely technology and healthcare. Alternative model specifications are proposed to test the robustness of the basic model, including dynamic models (with lagged dependent variables). The present study indicates that a higher proportion of structural capital (intangible assets, excluding goodwill) is a negative factor for company profitability in the technology and healthcare sectors. There is no indication that a more intense use of intangible assets and more investments in R&amp;D positively contribute to company profitability in the respective industries, for a large sample of listed companies. A higher proportion of intangible assets, as reported in financial statements, is possibly related to inefficiencies in the management of structural capital. The inverse relationship between profitability and investments in intangible assets is likely due to failures in cost accounting. Limitations and future research propositions are provided in the conclusions.