Since the 21st century, with the development of the Internet, online education has gradually become an important trend in the development of the education industry. The motivation of online learning has also received extensive attention and research in the academic community, but compared with the external motivation, the specialized research of internal motivation is less. Therefore, this paper will base on the influence of Bruner's intrinsic motivation theory on online learning, and analyse the relationship between three elements of internal motivation and synchronous online learning and asynchronous online learning. Combined with the existing online learning software and platform, this paper analyses and summarizes the promotion effect of internal motivation on online learning, and puts forward some own views and opinions.