Intramuscular Injection Of Testosterone Propionate Research Articles

Influence of an exogenous androgen, testosterone propionate on milt quality and short-term preservation of milt of Asian seabass Lates Calcarifer

Asian Seabass Lates calcarifer is widely used for pond and cage culture practice in the Kingdom of Saudi Arabia. A studywas carried out to understand the influence of an exogenous androgen, Testosterone propionate (TP) on milt quality ofL. calcarifer. Intramuscular injection of TP at dose 1.0 ml kg-1 was given to mature male fishes grown in a brood stockpond. A control was also maintained without giving hormone injection. After 36 h of injection, milt was collected and miltquality was assessed for colour, sperm cell concentration, motility and viability of spermatozoa. Short-term preservation ofmilt was carried out in oxygen and marine teleost Ringer’s medium at 4oC for 120 h. No significant change was observedin colour and pH of milt, but nature of milt changed from thick to watery due to hormone injection. Enhanced milt volume(14%) and sperm cell concentration (4%) was noticed in fishes from the treatment group 36 h post-injection, when comparedto control. Sperm motility duration and percentage motility were high in treatment fishes than control. Milt preserved inoxygen medium showed high sperm motility and viability when compared to Ringer’s medium. Intramuscular injection ofTP at 1.0 ml kg-1 improved milt volume and quality and therefore can be used for induced breeding of Asian Seabass. Keywords: Asian Seabass, Hormone, Intramuscular injection, Milt quality, Ringer’s medium, Sperm motility, Testosteronepropionate

Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia.

Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments.

Effects of Topically Applied Spironolactone on Androgen Stimulated Sebaceous Glands in the Hamster Pinna

The effects of spironolactone (5% SYC-201G, a preparation developed for clinical use in acne vulgaris by Searle Yakuhin K.K.), which is known to have antiandrogenic effects by competitively inhibiting dihydrotestosterone at androgen receptor sites, was topically applied to the androgen stimulated sebaceous glands of adult female golden hamsters. Androgen stimulation, induced by intramuscular injection of testosterone propionate (TP) every other day over a two week period, resulted in a 2.5 to 2.7 time increase in the size of the sebaceous glands of the hamster pinna. Once-daily treatment with 5% SYC-201G or matching placebo was applied to androgen-stimulated hamsters on one pinna only during the same period as TP injection. Comparison between the treated and untreated sides revealed a significant suppression in the sebaceous gland size (p < 0.05) by 5% SYC-201G; no such effect was observed with placebo. The difference in the suppression rate of the sebaceous gland size between 5% SYC-201G (23%) and matching placebo (-4.7%) was significant (p < 0.01).

Sequential Changes in the Prostate of Rats Treated with Chlormadinone Acetate, Testosterone and N-nitroso-N-Methylurea

An attempt was made to induce prostatic adenocarcinoma in rats. Temporary chemical castration of F344 rats was achieved by administration of diet containing 0.05% Chlormadinone acetate (CMA) for three weeks. The prostate was then stimulated by three consecutive daily intramuscular injections of testosterone propionate (100mg./kg.). On the day after the last testosterone injection, N-nitroso-N-methylurea (NMU) (50mg./kg.) was injected intravenously. Animals were sacrificed 10 days and 4, 20, 40 and 60 weeks after NMU treatment. Well-differentiated adenocarcinomas were obtained in three of fifty-four animals (5.6%) by this treatment and a high incidence of simple hyperplasia (88%) and atypical hyperplasia (19%) was also observed at 60 weeks. More studies are necessary to develop a protocol resulting in more rapid induction of prostatic adenocarcinomas at higher incidence.

Effects of photoperiod on formation of oestradiol-17 beta in the dove brain.

The effects of photoperiod and castration on brain aromatase activity have been examined using an in-vitro radioassay. Formation of oestradiol-17 beta was lower in the preoptic area of male Barbary doves on a short daylength (6 h light:18 h darkness) than in males on a long daylength (14 h light:10 h darkness). This was a specific effect of photoperiod which did not influence aromatase activity in the anterior or posterior hypothalamic areas, and was not accompanied by changes in hormone-sensitive vocal behaviour. Production of 5 beta-dihydrotestosterone, 5 beta-androstane-3 alpha, 17 beta-diol and 5 alpha-dihydrotestosterone by the preoptic area did not differ between birds on long or short days. Therefore, a short photoperiod does not appear to influence other pathways of androgen metabolism. In contrast to the effects of photoperiod, castration reduced oestradiol formation in both preoptic and hypothalamic areas. Intramuscular injection of testosterone propionate (TP) in intact males on short days did not restore the pattern of distribution of aromatase activity seen in males on long days. Preoptic aromatase activity was, however, restored by TP in castrated birds. We conclude that a short photoperiod influences both the activity of aromatase and the inductive effect of testosterone on enzyme activity in the preoptic area, which is known to be associated with the behavioural action of oestrogen in the dove. Photoperiod is likely to act both through changes in circulating androgen and by a direct action on preoptic cells.

The effect of testosterone on chemical carcinogenesis in the buccal pouches of castrated and intact male hamsters.

Tumors were induced in the cheek pouches of intact and castrated male Syrian golden hamsters by repeated topical application of 9,10-dimethyl-1,2-benzanthracene (DMBA). Half of the animals received in addition intramuscular injections of testosterone propionate throughout the treatment with DMBA. After 9 and 12 weeks of treatment, squamous cell carcinomas were reduced in number among intact animals receiving DMBA combined with testosterone. Among castrated animals treated with DMBA and testosterone, there were less tumors after 9 weeks than in castrated animals treated with DMBA only, but not after 12 weeks. The tumor incidence in intact and castrated animals treated with DMBA only was similar throughout the experiment. Thus in the present model testosterone appeared to decrease carcinoma formation in intact male animals.

ハムスター足底部におけるらい菌増殖増強法の検討

We have carried out to examine the influence of thymectomy and testosteroneadmini stration upon the growth of M. leprae in the foot-pads of golden hamsters.The golden hamsters were thymectomized by the suction of their thymus at 3 weeks of age, and were received an inoculation of M. leprae into their respective right hind foot-pads at 1-2 weeks after the thymectomy.The every day's intramuscular injection of testosterone propionate into each of their hind paws in the daily dosage of 0.2mg was continued for 6 days, at the first week, at 1 and 2 months after the inoculation. Thus, the total dosage was calculated to be 3.6mg per a hamster. Survival was good in all hamsters.The inoculum was 2.5×104 bacilli (SH-O passage strain) or 1.7×104 bacilli (B2409 P12 passage strain) per the each foot-pad.The details are shown in Table I. In the case of sham-thymectomized hamsters, the counts of M. leprae in each foot-pad rose to a plateau level of at most 106. However, in the case of the thymectomized and the testosterone-administered female hamster, they rose until a level of 1.6-4.3×107 at 24 weeks after the inoculation.Futhermore, standing on the cases hitherto tested, it has been suspected that the counts of M. leprae in the female hamsters were higher than those in the male ones. To sum up, it can be said that we have observed not only an enhance of susceptibility but a distinctly increased growth of M. leprae in the foot-pads of the golden hamsters. In addition, there appears to offer new approches to the study on the immunology of leprosy.

The Influence of Androgens in a Female Turkey

WITH numerous references to the influence of androgenic hormone in chickens, little mention is made of the responses in turkeys.One female Broad Breasted Bronze turkey was observed for a number of months while male sex hormone was being administered. This turkey was approximately six months old when the hormone was first given, and the treatment was continued for a little more than six months. Two pellets of testosterone were implanted subcutaneously, but adsorption apparently was so slow that intramuscular injections of testosterone propionate were given in addition. Injections were given twice weekly, and throughout most of the period the dosage was 8 mg. per injection. (Two female turkeys were started on the experiment, but fighting soon became so intense between them that one bird was removed from the pen and discontinued from the experiment.) The first indication of a response to the hormone was a slight reddening of Locals .

The Effect of Feeding Cow Manure on Egg Production and Hatchability

HAMMOND (1942) demonstrated that the feeding of dried cow manure increased the rate of growth of chicks and stimulated the development of their combs. After a more detailed investigation of the effect of feeding cow manure on the development of the combs, testes, and ovaries of chicks, Riley and Hammond (1942) reported that cow manure contained one or more androgenic principles. On the basis of these results the possibilities existed that egg production and hatchability would be favorably affected by the growth-stimulating dietary factors of cow manure or that they would be unfavorably affected by the androgenic constituents. It has been demonstrated that cessation of egg production is brought about by subcutaneous injection of a male hormone preparation in sesame oil (Schoeller and Gehrke, 1933), by intramuscular injection of testosterone propionate in sesame oil (Allee et al., 1939) or by implantation of testosterone propionate or androsterone pellets or intramuscular injection .

Testosterone Therapy of Male Eunuchoids

IN A series of papers (1, 2, 3, 4) the principal author and several associates have contributed evidence establishing the clinical effectiveness of various testosterone compounds in the treatment of a highly specific endocrine deficiency, primary male hypogonadism or eunuchoidism. In successive reports the various modes of administering testosterone have been discussed and compared with regard to the convenience of dosage necessary for originating improvement and the maintenance of the benefits achieved. These publications included convincing photographic (before and after treatment) proof that the intramuscular injection of testosterone propionate, the implantation of methyl testosterone pellets, the peroral swallowing of methyl testosterone tablets and the sublingual absorption of testosterone compounds dissolved in propylene glycol—all were successful in correcting the severe genital retardation of primary hypogonadism. In the last paper of this group (4) testosterone propionate, methyl testosterone an...

Oral Administration of Methyl Testosterone in Gynecology

THE USE OF ANDROGENIC HORMONES for the relief of varied functional gynecologic disorders has now been thoroughly accepted. That male sex hormone therapy has an established use in certain gynecologic disturbances is attested by the large number of clinical and experimental reports covering thousands of cases. The method of administration of these androgenic substances has been parenterally, either by the intramuscular injection of testosterone propionate in oil, the use of an active ointment through the skin, or more rarely, the subcutaneous implantation of pellets. The purpose of the present paper is to show that equally good results in the treatment of many gynecologic disturbances have been obtained by the oral administration of the synthetic and orally active androgenic hormone, methyl testosterone. It will be seen from the results of the present series of cases that the only therapeutic difference between the intramuscular injection of testosterone propionate and the oral administration of methyl test...

GONOPODIAL CHARACTERISTICS PRODUCED IN THE ANAL FINS OF FEMALES OF GAMBUSIA AFFINIS AFFINIS BY TREATMENT WITH ETHINYL TESTOSTERONE

Previous articleNext article No AccessJournal ArticlesGONOPODIAL CHARACTERISTICS PRODUCED IN THE ANAL FINS OF FEMALES OF GAMBUSIA AFFINIS AFFINIS BY TREATMENT WITH ETHINYL TESTOSTERONEC. L. TURNERC. L. TURNER Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by The Biological Bulletin Volume 80, Number 3June 1941 Published in association with the Marine Biological Laboratory Article DOIhttps://doi.org/10.2307/1537723 Views: 46Total views on this site Citations: 1Citations are reported from Crossref © 1941 Marine Biological LaboratoryPDF download Crossref reports the following articles citing this article: William N. Tavolga Effects of Gonadectomy and Hypophysectomy on Prespawning Behavior in Males of the Gobiid Fish, Bathygobius soporator, Physiological Zoology 28, no.33 (Sep 2015): 218–233.https://doi.org/10.1086/physzool.28.3.30159918

CLINICAL EFFECT OF THE ORAL USE OF METHYL TESTOSTERONE IN EUNUCHOIDISM1

THE EFFECTS of the administration of androgens in testicular deficiency have been well described in the past few years, clinically by McCullagh (1) and metabolically by Kenyon et al. (2). Androgens have heretofore been administered by intramuscular injection of testosterone propionate in oil, percutaneously and by subcutaneous pellet implantation (3). Foss (4) and McCullagh (5) described the effects of peroral methyl testosterone in testicular deficiency. The latter concludes that oral methyl testosterone is effective therapy in eunuchoidism and in terms of clinical symptoms its effectiveness as compared to testosterone propionate injections approximates three or four to one. He further states that the increase in urinary androgens following clinically effective doses of the propionate does not occur to the same degree after methyl testosterone. We have used oral methyl testosterone since January, 1940, for disorders in both male and female. Results, however, are being reported only in eunuchoidism since ...

THE EFFECTS OF TESTOSTERONE PROPIONATE IN IMPOTENCE

1. Eighteen men who complained of impotence and who had no obvious disease of the central nervous system, no marked structural changes in the genito-urinary tract except for absence of spermatozoa in Case 4, or no manifest deficiency in the internal secretion of the testes, were given intramuscular injections of testosterone propionate in 25 mgm. doses three times or more weekly. 2. In some of our patients psychic and emotional influences played a large part in the production of the impotence. 3. In seven instances relief from the impotence was obtained, while in the eleven other subjects the testosterone propionate did not produce any beneficial effects. 4. In one patient there was a definite reduction in the number of spermatozoa during the course of treatment. 5. It was concluded that the successful results were probably explained by psychological factors, since there was no decrease in potency when sesame oil was substituted and when all injections were stopped. 6. The amount of androgens administered...