Signs Of Intoxication Research Articles

Estimation of acute oral toxicity of the drug "Mastigard®"

The parameters of acute oral toxicity of Mastigard® are presented. The drug was introduced intragastrically to rats at a dose of 2000 mg/kg. During the study, the tested dose did not cause signs of intoxication and death of animals. Physiological parameters of rats remained within the species norm. Also no changes in the behavior of animals were noted, the reaction to tactile, pain, sound and light irritants was adequate. Autopsy of animals at the end of observation did not reveal pathological and anatomical changes in internal organs and tissues. Regular control of body weight of rats testified to uniform weight gain of all experimental animals during 14 days of observation, no body weight loss was observed during all periods of weighing. Thus, the LD50 of Mastigard® is more than 2000 mg/kg. Mastigard® belongs to the 5th class of toxicity according to the GHS and the 4th class of danger (substances of little danger) according to GOST 12.1.007-76.

Problematic aspects of conducting an examination of drivers for the state of intoxication in health care institutions and processing it’s results

The implementation of the course for the integration of Ukraine into the European Union resulted in the need to bring national legislation into compliance with European standards. Today, the problem of effectively detecting and recording cases of drivers operating vehicles under the influence of alcohol, drugs, or other intoxicating substances, which can impair attention and reaction speed, remains highly relevant.` It is emphasized that driving vehicles while intoxicated is one of the most dangerous administrative offenses, often resulting in irreparable consequences. The article is devoted to exploring the problematic issues concerning the legal regulation of medical examinations in cases of administrative offenses in Ukraine. Amendments are proposed to the Instruction on the procedure for detecting signs of alcohol, drug, or other intoxication in drivers of vehicles, or those under the influence of drugs that reduce attention and reaction speed. This Instruction was approved by the order of the Ministry of Internal Affairs of Ukraine, the Ministry of Health of Ukraine dated November 9, 2015, No. 1452/735. The rationale is substantiated for extending the time allowed for the examination of drivers of vehicles who need to be assessed in healthcare institutions to ascertain their state regarding alcohol, drug, or other intoxication, as well as proposing changes and additions to clause 9 of Section II - ‘Conducting an examination of the state of alcohol intoxication by a police officer and registration of its results’ of the Instructions. The article highlights the issue of inadequate legislative regulation of the procedure for recording the results of the examination, laboratory tests, and diagnosis, particularly concerning the registration of medical examination results to detect the presence of driver intoxication.

"Catch-up" therapy: combining antidotal treatment with dermal application of AHA following percutaneous VX poisoning in the domestic swine.

Low-volatility organophosphorus chemical warfare agents (OP CWAs) are cholinesterase inhibitors which easily absorb into the skin, leading to the formation of a dermal depot from which they slowly enter the bloodstream. This leads to sustained cholinergic hyperstimulation, which if untreated may lead to death. However, current available countermeasures are not adequate to neutralize the agent residing in the dermal depot. Accordingly, we evaluated the efficacy of the potassium salt of acetohydroxamic acid (880 mg/ml in DMSO/H2O 1:4, AHAK), as a potential "catch-up" therapy lotion intended to neutralize the dermal depot, by penetrating the skin and decomposing it before it reaches the bloodstream. To that end, we compared the clinical outcome following skin surface decontamination combined with antidotal treatment, to that following the same antidotal treatment combined with dermal application of AHAK at the site of VX exposure, against percutaneous poisoning by a lethal neat dose (4 mg/kg) of the low-volatility nerve agent VX, in an unanesthetized swine model. Following skin surface decontamination and antidotal treatment, recurrence of intoxication signs and a prolonged recovery time were observed. In contrast, similar antidotal treatment combined with dermal application of AHAK significantly reduced intoxication signs recurrences and accordingly medical supervision duration needed, paralleled by a significantly faster recovery of whole blood cholinesterase activity. An initial evaluation demonstrated the safety of prolonged whole-body AHAK application. Hence, the AHAK lotion may act as an efficient "catch-up" therapy against percutaneous poisoning by low-volatility OP CWAs, improving the clinical outcome and reducing the burden on medical staff.

Clinically significant and colonizing <em>Enterobacteria</em> isolates with extended spectrum beta-lactamase production and their significance in horse breeding

This review article is devoted to the problem of the emergence and spread of antibiotic-resistant enterobacteria producing beta-lactamase in horse breeding. Foal colibacillosis is an acute infectious disease of young animals, manifested by diarrhea, signs of severe intoxication and dehydration of the body, caused by pathogenic Escherichia coli. Foals get sick in the first 3-7 days after birth. Infection occurs alimentary, less often – aerogenic and intrauterine transmission. The source of infection are sick and recovered animals. The duration of the disease is 2-3 days with a mortality rate of up to 90%, which leads to multiple economic losses. Multiresistant Escherichia coli can accumulate in the intestines of healthy animals and be excreted with fecal matter into the environment, which leads to the spread of pathogenic microflora among livestock. In this case, the animals are latent or hidden carriers that do not show clinical signs of the disease. Clinically significant isolates are causative agents of infectious and inflammatory diseases. These microorganisms represent a potential hazard for the development of an infectious process, they exhibit virulent properties that lead to damage to the tissues of the host organism, which significantly limits the possibility of their eradication. Along with clinically significant isolates, there are colonizing isolates that do not cause infection at a specific site. It should be noted that under certain conditions, the colonizing isolate can cause an infectious process. Irrational use of antimicrobials is a key reason for the development of tolerance. Also, the untimely detection of enzyme inactivation of antibiotics by laboratory methods and the spread of resistance genes play an important role. Currently, disks and media with antibacterial drugs are widely used to detect multidrug resistance of microorganisms. Along with these methods, PCR diagnostics and sequencing are used to accurately determine antibiotic resistance genes. Due to the importance and relevance of this topic, many scientists are conducting research to detect and study the multiresistance of enterobacteria such as E. coli, and their spread among animals.

Biomarkers of some commercial lotion and shampoo based pesticides toxicity in male mice

The effects of Quick (0.5% malathion) and Licid (0.6% tetramethrin with 2.4% piperonyl butoxide) lotions, and Newcid shampoo (1% permethrin) applied on the whole-body of male mice was investigated. Newcid and Licid treatments induced signs of toxicity characterized by tremor, salivation and diarrhea, while Quick lotion produced signs of intoxication characterized by nausea, vomiting, seizures, convulsions, salivation and lacrimation.

Evaluation of Biocompatibility of New Osteoplastic Xenomaterials Containing Zoledronic Acid and Strontium Ranelate

Background. The problem of improving the functional characteristics of implanted devices and materials used in traumatology and orthopedics is a topical issue.
 Aim of the study to study biocompatibility of bovine bone matrix xenomaterials modified by zoledronic acid and strontium ranelate when implanted into the bone defect cavity.
 Methods. The study was performed on 24 male rabbits of the Soviet Chinchilla breed. Test blocks of bone matrix were implanted into the cavity of bone defects of the femur. Group 1 animals (n = 8, control group) were implanted with bone xenogenic material (Bio-Ost osteoplastic matrix). Group 2 animals (n = 8) were implanted with bone xenogenic material impregnated with zoledronic acid. Group 3 animals (n = 8) were implanted with bone xenogeneic material impregnated with strontium ranelate. Supercritical fluid extraction technology was used to purify the material and impregnate it with zoledronic acid and strontium ranelate. Radiological, pathomorphological, histological and laboratory (hematology and blood biochemistry) diagnostic methods were used to assess biocompatibility. Follow-up period was 182 days after implantation.
 Results. It was found out that on the 182nd day after implantation the median area of the newly-formed bone tissue in the defect modeling area in Group 1 was 79%, in Group 2 0%, in Group 3 67%. In Group 2 the maximum area by this period was filled with connective tissue 77%. Median relative area of implanted material fragments in Group 1 was 4%, in Group 2 23%, in Group 3 15%. No infection or material rejection was observed in animals of all groups. There were no signs of intoxication or prolonged systemic inflammatory reaction. Laboratory parameters did not change significantly over time. One animal in each group experienced one-time increase in C-reactive protein level against the background of leukocytosis. Two animals in Group 1 had a slight migration of implanted material under the skin, one animal developed arthritis of the knee joint.
 Conclusion. Osteoplastic materials based on bovine bone xenomatrix and filled with zoledronic acid and strontium ranelate have acceptable values of biocompatibility including their safety profile.

Behavioral and physiological response of the passerine bird Agelaioides badius to seeds coated with imidacloprid.

Neonicotinoids are globally used insecticides, and there are increasing evidence on their negative effects on birds. This study is aimed at characterizing the behavioral and physiological effects of the neonicotinoid imidacloprid (IMI) in a songbird. Adults of Agelaioides badius were exposed for 7days to non-treated peeled millet and to peeled millet treated with nominal concentrations of 75 (IMI1) and 450 (IMI2) mg IMI/kg seed. On days 2 and 6 of the trial, the behavior of each bird was evaluated for 9min by measuring the time spent on the floor, the perch, or the feeder. Daily millet consumption, initial and final body weight, and physiological, hematological, genotoxic, and biochemical parameters at the end of exposure were also measured. Activity was greatest on the floor, followed by the perch and the feeder. On the second day, birds exposed to IMI1and IMI2 remained mostly on the perch and the feeder, respectively. On the sixth day, a transition occurred to sectors of greater activity, consistent with the disappearance of the intoxication signs: birds from IMI1 and IMI2 increased their time on the floor and the perch, respectively. Control birds always remained most of the time on the floor. IMI2 birds significantly decreased their feed intake by 31% the first 3days, compared to the other groups, and significantly decreased their body weight at the end of the exposure. From the set of hematological, genotoxic, and biochemical parameters, treated birds exhibited an alteration of glutathione-S-transferase activity (GST) in breast muscle; the minimal effects observed are probably related to the IMI administration regime. These results highlight that the consumption of less than 10% of the bird daily diet as IMI-treated seeds trigger effects at multiple levels that can impair bird survival.

Toxicological characteristics of the probiotic drug Bioseven

Ukraine's food security is determined by the production of a sufficient number of high-quality, ecologically harmless, complete food products of animal origin. Bacterial preparations based on live microbial cultures - pre and probiotics - have become widely used in most countries of the world, including Ukraine, in the technological process of growing agricultural animals, especially poultry. The purpose of the work was to conduct a toxicological characterization of the probiotic preparation Bioseven. The following research methods were used to obtain scientific information: zootechnical, zoohygienic, morphological, biochemical, statistical. The research was conducted on white Wistar rats of both sexes. Healthy animals with the ap propriate body weight were used in the experiments. Fluctuations in body weight in the respective groups did not exceed ±10.0 %. Animals were kept in groups in cages in compliance with sanitary and hygienic requirements. It was established that when Bioseven was administered to white rats in doses of 1000, 2500, 5000 mg/kg, all animals remained alive and clinically healthy: the behavior of the animals was typical for this species of rodents. Activity, grooming, breathing rate, feed and water consumption in all groups did not differ significantly and were within physiological norms, no signs of intoxication were registered. Under the conditions of intragastric administration of the drug Bioseven LD 50 for white mice and rats is more than 5000 mg/kg of body weight. The use of the drug Bio-seven in the above doses for 30 days leads to kidney and liver damage in the animals of the third experimental group (10-fold dose). The application of the drug Bioseven to the animals of the two experimental groups did not show probable changes in the morphological and biochemical indicators of the blood compared to the control. The level of endogenous intoxication of the body of white rats (SZE) did not change between experimental animals. Thus, the toxicological characteristics of the probiotic preparation Bioseven indicate the absence of a suppressive effect of the probiotic Bioseven on the metabolic processes of the body of laboratory animals. Key words: probiotic, toxicological characteristics, suppressive effect, biochemical, ecologically harmless, complete, food products, animal origin, food safety, consumer.

Levamisole hydrochloride as an anthelmintic against Neoechinorhynchus buttnerae in juvenile Colossoma macropomum.

Acanthocephalosis is caused by the endoparasite Neoechynorhyncus buttnerae and affects fish farmed in the Amazon. This study assessed the efficacy of therapeutic levamisole hydrochloride (LVC) baths against N. buttnerae and its effects on juvenile tambaqui blood parameters. In vitro and in vivo tests were carried out, the latter employing two experimental therapeutic LVC bath protocols. Concerning in vitro efficacy, the T75 (75 mg.L-1 LVC) and T100 (mg.L-1 LVC) treatments were 100% effective in 15 min, while the T50 (50 mg.L-1 LVC) and T25 (25 mg.L-1 LVC) treatments required parasite exposure for 45 and 60 min, respectively. During exposure, the parasites displayed reduced motility, proboscis retraction, coiling into a spiral shape, body rigidity and swelling. The LVC LC50-72h for juvenile tambaqui was 115 mg.L-1 . Regarding in vivo efficacy for Protocol I (8-h bath), the T125 resulted in 82% effectiveness, while in Protocol II (two 8-h baths with a 24-h interval), the T115 treatment (115 mg.L-1 LVC) achieved 95.6% effectiveness without clinical intoxication signs, despite behavioural changes. No significant changes were observed in fish blood parameters. LVC was, therefore, highly effective both in vitro and in vivo in controlling the acanthocephalan N. buttnerae without compromising tambaqui juvenile homeostasis.

Study of acute toxicity of GIZh-298

Relevance. Assay of acute toxicity is a mandatory step in a preclinical safety study of medicines and pharmaceutical substances. The purpose of this study was to determine the parameters of acute toxicity of the substance GIZH-298. Methods. GIZH-298 was administered once orally to outbred mice of both sexes at doses of 350–550 mg/kg. Control mice received 0.5 ml of 1 % starch solution under the same conditions. During the experiment, the death of animals, signs of intoxication were observed, and the clinical picture was recorded. Pathological anatomical autopsy of the dead mice was performed as they died. Surviving mice were autopsied 14 days after the start of the experiment, immediately after their euthanasia. Results. The average lethal doses of the GIZh-298 substance when administered orally to mice were determined: LD50 in females was 356 mg/kg, LD50 in males was 438 mg/kg. Conclusion. Compound GIZH-298 for oral administration according to the classification of Sidorov K.K. (1973) is a moderately toxic substance and can be assigned to the 3rd class of toxicity, and in accordance with GOST 12.1.007-76 — to the 3rd hazard class.

Hematological, Biochemical and Histological Analysis on Wistar Rats Treated by an Herbal Medicinal Product "DAOUTRA EPIGASTRO” Marketed in Daloa City (Côte d'Ivoire)

Herbal products developed by traditional healers are more and more in increasing in Africa. Unfortunately, there is often no safety data on these products. That is the case of an Herbal Medicine Product (HMP) called "DAOUTRA EPIGASTRO", widely used by local population in Daloa City-Cote d’Ivoire to treat chronic gastritis without knowing its harmlessness. This study aimed to study safety of this product by evaluating its acute and sub-acute effects on Wistar rats by oral administration. Acute toxicity test was assessed with a single concentration of either 500, 1000 and 3000 mg/kg bw of the product to female rats in the goal to determine clinical signs of intoxication and LD50. However, sub-acute toxicity test was carried out by daily administration of 300, 500 and 1000 mg/kg bw for 28 days duration in order to identify eventual modifications of hematological and biochemical parameters; then to appreciate histological changes of some selected intern organs. This operation was achieved by collecting blood and isolating hearts, livers and kidneys at the end of the experiment. As results, acute study showed that from day 7 onwards, the remedy administered at 3000 mg/kg significantly increased the weight of the rats compared to the controls, no mortality, no clinical sign of intoxication and LD50 was higher than 3000 mg /kg bw. For sub-acute exposure, the animals showed a stimulation of the appetite for food after consumption of the remedy "DAOUTRA EPIGASTRO". Except the increasing of AST and ALT content at 1000 mg/kg bw, no abnormal modifications were observed for hematological and biochemical parameters, then histological analysis didn’t show any alteration of selected organs. In addition, the difference in relative weights between treated and control rats were not statistically significant for the liver and heart. However, there was a significant difference between relative weights of liver at all treated rats in comparison to control. The study showed that the .....

Berberine-loaded zein/hyaluronic acid composite nanoparticles for efficient brain uptake to alleviate neuro-degeneration in the pilocarpine model of epilepsy

Berberine hydrochloride is a plant alkaloid with versatile medicinal applications, yet it has suffered from multiple limitations in its usage. Nonetheless, the acknowledged role of berberine in controlling seizures has fuelled the need to develop a nanosystem capable of delivering it safely and efficiently to the brain. Consequently, zein and hyaluronic acid were chosen for this purpose, and about twenty formulations with different preliminary factors were screened. Afterward, three promising formulations were loaded with berberine and characterized to select an optimum formulation for further in vivo inspection. The B2 formula of particle size of 297.2 nm ± 1.86 and % entrapment efficiency of 83.75% ± 1.39 has succeeded in the increment of the brain uptake of berberine. Moreover, compared to free berberine suspension, the severity of pilocarpine-induced status epilepticus in rats was depleted after the subcutaneous administration of B2. The hippocampal tissue of rats receiving B2 showed signs of reduced neuro-degeneration, remarkably lower expression levels of COX-2 and TNF-α, and enhanced antioxidant activity. Finally, the relative safety of the developed system was determined after searching for any sign of intoxication or behavioral changes. In conclusion, the developed berberine loaded composite nanoparticles successfully delivered berberine across the BBB securely to ameliorate the deteriorating impact of pilocarpine-induced epilepsy.

Road Safety in the Arctic Zone of the Russian Federation

The article deals with the issue of road safety in the Arctic zone of the Russian Federation.The analysis of road traffic accidents over the past 10 years that occurred in the Arctic zone of the Republic of Sakha (Yakutia) was carried out for the first time ever. The study refers to the total number of road traffic accidents, accidents due to the fault of pedestrians, accidents due to the unsatisfied condition of streets and roads, accidents involving children under 16 years of age, accidents involving drivers with signs of alcohol intoxication and accidents committed by drivers without the right to drive vehicles. Based on the data obtained, a coefficient K was proposed that characterises the state of road safety in the Arctic regions in comparison with traffic safety in general in the Republic of Sakha (Yakutia).The analysis of road traffic accident statistics has shown that many accidents are the fault of drivers without a driver’s license and revealed the districts with respectively the highest and lowest number of accidents as per their types listed above.Despite the huge territory and the small number of inhabitants, and consequently the low population density, the number of accidents in the Arctic zone of the Republic of Sakha (Yakutia) has been increasing every year necessitating efforts to improve road safety.

Исследование переносимости препарата БАРС® капли инсектоакарицидные для кошек

Ectoparasites are a serious problem for veterinarians and pet owners.
 Insectoacaricidal preparation BARS®, developed by NVC Agrovetzashchita LLC, is a multicomponent insectoacaricide and is produced in the form of drops at the withers. For cats as active substances in 1 ml contains: fipronil ‒ 100 mg, cyfluthrin ‒ 2.5 mg, piperonyl butoxide ‒ 0.5 mg, diflubenzuron ‒ 1 mg.
 Purpose of the study. To study the tolerability of the drug during its repeated use in cats at a therapeutic dose of 0.5 ml/5 kg and a double therapeutic dose of 1 ml/5 kg of body weight with daily skin application for 7 days.
 
 Materials and methods. To study the tolerability of the Insectoacaricidal preparation BARS®, 3 groups of cats were formed, 10 animals each (2 experimental and 1 control).
 Results. During the experiment, there were no external signs of intoxication in cats. All animals of the experimental groups were active. The reaction to external stimuli is preserved. The body temperature of the animals throughout the experiment remained within the physiological range in all groups.
 Conclusion. The drug in recommended doses is well tolerated by cats, does not adversely affect the general physiological state, morphological and biochemical parameters of blood, and physical and chemical parameters of urine. Also, they do not have a skin-resorptive and locally irritating effect.

Morphological Aspects of Intestinal Failure in Experimental Peritonitis And Correction

Our studies show that pathomorphological changes in the small intestine in acute experimental peritonitis are characterized by dystrophic, inflammatory-destructive and vascular disorders in the vascular tissue structures of the small intestine wall. In the pathogenesis of damage to the small intestine, deep inflammatory and destructive changes in the vessels of the microhemocirculatory bed, which subsequently lead to disruption of cell trophism, tissue hypoxia, damage to cellular elements, disruption of cellular metabolism, deficiency of energy and plastic materials, accumulation of perverted metabolic products in cells and tissues, are of great importance. . The use of the “Zerotox“ enterosorbent in the treatment complex for the treatment of endogenous intoxication and toxemia had a positive effect on the morphofunctional status of the small intestine, on the clinical course of the disease, on the composition of the intestinal microflora, indicators of local immunological protection, improved clinical and laboratory signs of endogenous intoxication, reduced the recovery period bowel function, reduced the number of postoperative complications

Reasons for unsatisfactory results of treatment of destructive forms of erysipelas

Goal. Find out the cause of unsatisfactory treatment outcomes in patients with destructive forms of erysipelas.
 Materials and methods. Retrospectively and prospectively analyzed 284 case histories of patients who were hospitalized in the center of purulent - septic surgery KNP "City Hospital G3" Zaporizhzhia for the period 2016-2022.
 Results. It was found that most often the local focus was localized on the upper and lower extremities. The most common clinical manifestations of intoxication in bullous and phlegmonous forms of erysipelas were: general weakness, hyperthermia, muscle pain. In the necrotic form of erysipelas, patients showed signs of severe intoxication with nausea, vomiting and confusion. In the complex treatment of patients with erysipelas, the main place belongs to the early surgical treatment of the area of ​​the pathological process and antibiotic therapy (ABT).
 Conclusions. Radical surgery and timely targeted ABT are key elements of success in the treatment of surgical forms of erysipelas and are not subject to revision. The main causes of unsatisfactory consequences in patients with erysipelas are: late referral of patients to the hospital, aggravation of the disease at the time of hospitalization by septic shock and multiple organ failure, severe decompensated comorbidities.

SUB-ACUTE DERMAL TOXICITY OF SODIUM-CONTAINING DRUGS

Drugs that have antimicrobial, anti-inflammatory and wound-healing effects deserve special attention, sodium-containing drugs are one of them, which is why they are used in veterinary medicine.
 The article presents the results of experimental studies to study the sub-acute toxicity of sodium-containing drugs: known – glycetinate 2%, its analogues – ovocid 2%, ovocid 3%, potassium-sodium 1.5% (Na / K, 1.5%).
 The study of sub-acute toxicity of drugs was carried out by applying drugs to the skin of animals. The experiment used white rats weighing 250-270 g. 4 groups of rats with 3 animals in each were formed: rats of group I on the skin was applied ovocid 3%, group 2 - Na / K drug 1.5%, 3rd group – ovocide 2%, and the 4th group of rats (control) was applied glycetinate 2%. The animals were subjected to clinical observations, assessing their general condition and skin reaction. The effect of drugs on morphological and biochemical parameters of blood of rats was studied.
 Evaluating the general condition, behavior, local skin reaction of rats for the study of subacute toxicity, local irritant action of sodium-containing drugs, it was noted that they did not cause visible violations of physiological functions, the appearance of clinical signs of intoxication. No significant clinical signs of inflammation or irritation (erythema, edema, and cracks) were recorded on the skin of the animals. At the same time, it should be noted that the animals of all experimental groups had a slight redness at the place of application of the drugs, which later disappeared.

The impact of tableside ordering technologies on alcohol sales to the intoxicated.

The impact of tableside ordering technologies on alcohol sales to the intoxicated.

Регламентування у повітряному середовищі біоцидного препарату «LONZABAC GA» за маркерною леткою сполукою

Lonzabac GA (active ingredient – cocopropylenediamineguanidine acetate) is used as basic component in manufacturing surface and medical instrument disinfectants. In Ukraine, disinfectants are subject to sanitary standardization and obtaining of marketing authorization. Given that the active ingredient Lonzabac GA is non-volatile, the question came up of detecting volatile compounds that can get into the air during its use, with subsequent establishment of a marker compound to be used to monitor the air quality. The objective of this paper was to study the toxicity of LONZABAC GA at laboratory animals with various routes of exposure as part of sanitary standardization and to justify the drug administration standard based on marker volatile compound. To this end the sanitary and chemical, toxicological, and statistical research methods were used. Results: Lonzabac GA classifies as hazard class 3 in terms of acute oral toxicity. It has no skin resorptive effect. Lonzabac GA has dermal and mucous membranes irritating action. Significant cumulative activity. It cause no body sensitization. Lonzabac GA, when used as disinfectant component, does not form drug aerosol, instead, an isopropyl alcohol migrates from the drug into the air. No LС50 was reached in acute ingestion experiment. The experimental animals showed no clinical signs of intoxication, changes in biochemical and hematological parameters during chronic inhalation study. Conclusions: Therefore, it is advisable to monitor air quality both during manufacture and use of Lonzabac GA based on concentration of marker compound – isopropyl alcohol.

Preclinical Evaluation of a New Anti-COVID-19 Intravenous Immunoglobulin

Introduction. Using of immunoglobulins containing SARS-CoV-2 neutralizing antibodies may be an effective and safe tool for COVID-19 treatment. An intravenous immunoglobulin COVID-globulin from donor blood plasma containing SARS-CoV-2 neutralizing antibodies was developed at Joint-Stock Company Nacimbio.Aim. A pilot study of the safety of the "COVID-globulin".Materials and methods. When studying the safety of the preparation in animals the following parameters were evaluated: general toxicity, thrombogenic potential, influence on hematological and biochemical parameters, blood clotting and hemolytic activity, determination of local irritant action, pyrogenic properties, bacterial endotoxins, allergic effect of the drug preparation and its physicochemical characteristics.Results and discussion. Safety studies of "COVID-globulin" in animals showed no signs of intoxication, local irritant action and thrombogenic properties. Macroscopic and histological examination of the organs of rats treated with COVID-globulin showed no signs of necrosis, inflammation, atypia, or any significant pathological changes. Hematological and biochemical parameters of the blood of laboratory animals after the administration of "COVID-globulin" corresponded to the reference values. Administration of COVID-globulin to rabbits did not activate blood clot formation. The IgG subclasses distribution in the preparation corresponded to that in human plasma. The activity of the Fc-function of the immunoglobulin molecule was more than 130 % compared to the reference standard preparation, the concentration of the prekallikrein activator in the COVID-globulin ranged from 4.2 to 4.8 IU/ml, anticomplementary activity was less than 1 unit complement per 1 mg of protein.Conclusion. The results of all studies have demonstrated a high level of safety of the developed COVID-globulin preparation, which meets the safety requirements for human immunoglobulins for intravenous administration by national regulatory documents and the European Pharmacopoeia.