Abstract Introduction Poor sleep quality has adverse effects ranging from decreased focus to increased work-related injuries. The Perceptual Vigilance Task (PVT), a measure of reaction time (RT) used to assess alertness is commonly used in sleep research. This study focuses on the relationship between dysfunctional sleep-related cognitions (Dysfunctional Beliefs and Attitudes About Sleep Scale [DBAS]), insomnia severity (Insomnia Severity Index [ISI]), and sleepiness (Epworth Sleepiness Scale [ESS]) and their association to PVT RT. Methods 162 participants were recruited from East Carolina University. Inclusion criteria: right-handed adults; exclusion criteria: history of brain injury, seizure disorder, or vision impairment. Age range 18–39 (M = 20.15; SD = 3.01); 81 (49.1%) females. Results ISI was correlated with PVT RT for inter-stimulus interval delay times of 1000ms r(162) = .155, p =.05, 2000ms r(162) = .204, p = .009, 5000ms r(162) =.164, p = .04, and 6000ms r(162) = .181, p = .02. DBAS was correlated with PVT RT for delay times of 2000ms r(162) = .204, p =.021, 3000ms r(162) = .160, p = .04, 4000ms r(162) = .170, p = .03, 6000ms r(162) = .171, p = .030, 7000ms r(162) = .219, p =.005, and 8000ms r(162) = .158 p = .045. ESS was not correlated with PVT. A regression was calculated to predict reaction time at 7000ms delay based on the DBAS (F(1,151) = 2.51, p = .01), with an R2 of .12. Conclusion There is a diminishing association found between insomnia severity and RT during inter-stimulus delay times (>6000ms). Dysfunctional beliefs about sleep correlate with RT through 8000ms delay, eventually predicting RT. Regardless of severity of sleep disturbance, sleep-related bias may affect subjective feelings of wakefulness and objective levels of alertness (e.g., one who believes they are not obtaining sufficient sleep may act accordingly). Support N/A