We reported a large-scale Internet-based experiment to investigate the impact of emotion information on Chinese word segmentation, in which participants completed an overlapping ambiguous string (OAS) segmentation task and the Chinese version of Beck Depression Inventory-II in a counterbalanced order. OAS is a three-character string (ABC) in which the middle character can form a distinct word with both the character on its left side (word AB) and the character on its right side (word BC). Participants were presented with isolated OASs and were asked to report the word they identified first. Emotional OAS was constructed by a combination of a neutral word and an emotional word, with the neutral and emotional words sharing character B. We orthogonally manipulated the valence of the emotional words (positive vs. negative) and their position in the OAS (left-side vs. right-side). The results showed that compared with neutral words, both positive and negative words were more likely to be segmented, and this segmentation outcome was not affected by readers with different depression tendencies. These findings suggest that emotion information can influence word segmentation, and that both positive and negative words take precedence over neutral words in the word segmentation process. This study provides a new perspective and evidence to understand the impact of emotion information on word processing.