Changes In Internal Structure Research Articles

Radiomics nomogram for prediction of glypican-3 positive hepatocellular carcinoma based on hepatobiliary phase imaging.

The hepatobiliary-specific phase can help in early detection of changes in lesion tissue density, internal structure, and microcirculatory perfusion at the microscopic level and has important clinical value in hepatocellular carcinoma (HCC). Therefore, this study aimed to construct a preoperative nomogram for predicting the positive expression of glypican-3 (GPC3) based on gadoxetic acid-enhanced (Gd-EOB-DTPA) MRI hepatobiliary phase (HBP) radiomics, imaging and clinical feature. We retrospectively included 137 patients with HCC who underwent Gd-EOB-DTPA-enhanced MRI and subsequent liver resection or puncture biopsy at our hospital from January 2017 to December 2021 as training cohort. Subsequently collected from January 2022 to June 2023 as a validation cohort of 49 patients, Radiomic features were extracted from the entire tumor region during the HBP using 3D Slicer software and screened using a t-test and least absolute shrinkage selection operator algorithm (LASSO). Then, these features were used to construct a radiomics score (Radscore) for each patient, which was combined with clinical factors and imaging features of the HBP to construct a logistic regression model and subsequent nomogram model. The clinicoradiologic, radiomics and nomogram models performance was assessed by the area under the curve (AUC), calibration, and decision curve analysis (DCA). In the validation cohort,the nomogram performance was assessed by the area under the curve (AUC). In the training cohort, a total of 1688 radiomics features were extracted from each patient. Next, radiomics with ICCs<0.75 were excluded, 1587 features were judged as stable using intra- and inter-class correlation coefficients (ICCs), 26 features were subsequently screened using the t-test, and 11 radiomics features were finally screened using LASSO. The nomogram combining Radscore, age, serum alpha-fetoprotein (AFP) >400ng/mL, and non-smooth tumor margin (AUC=0.888, sensitivity 77.7%, specificity 91.2%) was superior to the radiomics (AUC=0.822, sensitivity 81.6%, specificity 70.6%) and clinicoradiologic (AUC=0.746, sensitivity 76.7%, specificity 64.7%) models, with good consistency in calibration curves. DCA also showed that the nomogram had the highest net clinical benefit for predicting GPC3 expression.In the validation cohort, the ROC curve results showed predicted GPC3-positive expression nomogram model AUC, sensitivity, and specificity of 0.800, 58.5%, and 100.0%, respectively. HBP radiomics features are closely associated with GPC3-positive expression, and combined clinicoradiologic factors and radiomics features nomogram may provide an effective way to non-invasively and individually screen patients with GPC3-positive HCC.

Identification of Chemical and Structural Characteristics of Acrylic Paint Layer Using Terahertz Metasurfaces.

The precise investigation and monitoring of the internal structural change within complex layered systems are crucial, as the emergence of undesirable defects or formation of secondary internal structures significantly exerts a profound influence on the overall properties of the system. We demonstrate an advanced sensing platform utilizing terahertz metasurfaces, allowing chemical detection and precise identification within an acrylic paint layer with a noticeable sensitivity, reaching down to several hundreds of nanometers, in nondestructive and noncontact manners. The identification of solid and mixed paint samples was achieved by analyzing their optical properties, including the refractive index and absorption coefficient. Notably, the presence of internal pore defects within the mixed acrylic paint led to geometric distortions, affecting the state of the overall system. Intriguingly, even in cases where acrylic paint exhibited identical colors perceptible under visible light, distinct discrimination and identification of chemical compositions were successfully proposed.

Subsonic and supersonic polaron dynamics in polyacetylene

The paper considers the dynamics of a polaron in an electric field on a polyacetylene lattice. The one-electron non-adiabatic approximation based on the Su–Schrieffer–Heeger model is used. In an electric field, a polaron gains one of two saturated velocities, — subsonic and supersonic. Velocity bifurcation occurs in a very narrow range of electric field strengths. In an electric field, the polaron’s internal dynamic and electronic structure changes in a complex way. The supersonic polaron transforms the energy received from the electric field into monochromatic lattice oscillations. The generation of these oscillations is due to the internal polaron dynamics. The effective polaron mass is determined and m* ≈ 1.3. The negative effective mass was also identified. An analysis of the wave function and its phases provides useful information about the polaron evolution. The polaron dynamics is also studied depending on the mode of switching on and off the electric field and in multiple electric fields.

Physically Present but Spiritually Distant: The View of the European Union in Poland

AbstractThe Polish people remain staunchly in favour of the concept of a European Union. Paradoxically though, there has been strong and continued electoral support for Law and Justice (PiS), its ruling party, despite its insistence on precipitating and then continuing multifaceted conflicts with EU institutions. This article examines the internal structural changes in Poland and the attitudes to the EU of Poland’s leaders; the article will argue that those attitudes have deepened pre-existing divisions over integration. By fusing the triple modernization theory of European integration with a two-dimensional concept of party-based Euroscepticism, the article shows how PiS’s ambiguous discourse on European integration, combined with an increasingly instrumental approach to the EU by the Polish electorate—and that electorate’s deepening polarization—have secured steady support for PiS from ostensibly Europhile voters.

Microstructural study of apple slices during hot air‐explosion puffing drying

AbstractIn order to reveal the change laws of microstructure in apple slices during hot air‐explosion puffing drying, the microstructure images and parameters of apple slices under different drying conditions were obtained by using paraffin sectioning, microscopic observation, and image processing techniques with apples as raw material and CO2 as drying medium. Subsequently, the changes in internal cell and pore structure of dried apple slices were analyzed, as well as the relationship between micro‐parameters of apple slices and macro‐measurement indicators was further obtained. Results indicated that the overall cross‐sectional area, cross‐sectional perimeter, equivalent diameter, and porosity of cells increased by explosion puffing drying, while the cell roundness and wall roughness factors changed relatively little. Meanwhile, macro–micro parameters of expanded apples were fitted and analyzed. Then, the obtained mathematical model showed a nonlinear correlation as experimental and predicted results exhibited good agreement, with determination coefficients R2 more significant than 0.8, which could better expect the macro–microparameters to change in the drying process. The results of this research could provide an essential reference for exploring the change laws of tissue microstructure parameters in apples dried by hot air‐explosion puffing drying, with expansion and rehydration ratios under different experimental conditions, as well as optimizing the puffing drying process and designing corresponding drying equipment.Practical ApplicationsFresh apples have high moisture content and are not easy to store, so drying is usually used to reduce the moisture content to extend shelf time and increase economic benefits. For the drying process of fruit and vegetable products, scholars mainly focused on the influence of microstructure parameters on moisture migration, drying rate, heat and mass transfer and other factors, but did not give a quantitative description of how microstructure parameters affect macroscopic parameters. In this article, the relationship between microstructural changes and macroscopic indicators was investigated by taking puffed apple slices as an example. Research could provide an essential reference for exploring the change laws of tissue microstructure parameters in apples dried by hot air‐explosion puffing drying, with expansion and rehydration ratios under different experimental conditions, as well as optimizing the puffing drying process and designing corresponding drying equipment.

In situ and offline mapping analyses of fatigue behavior in carbon-fiber-reinforced polymers by small- and wide-angle X-ray scattering

Although carbon-fiber-reinforced polymers (CFRPs) have excellent mechanical properties and are considered sustainable, an improved understanding of the fatigue behavior of these materials is crucial for expanding their application scope. The aim of this study is to clarify the fatigue mechanism of CFRPs using in situ and offline mapping by small-angle/wide-angle X-ray scattering (SAXS/WAXS) measurements. The resulting findings provide information about the interface between the carbon fibers (CFs) and epoxy matrix, as well as changes in epoxy chain structure and internal structure of the CFs. For example, the in situ SAXS profiles revealed an increase in intensity with cycling, which may be related to void and crack formation. Furthermore, the in situ WAXS profiles indicated that the 0° fibers became less ordered and that some destruction of the turbostratic structure occurred. Offline SAXS/WAXS mapping after fatigue tests with low and high loads revealed information about changes in the epoxy resin and carbon fibers, respectively, as well as the shapes of cracks. In particular, offline mapping confirmed that epoxy chain relaxation occurred rapidly after failure.

First-principles study on the specific heat jump in the glass transition of silica glass and the Prigogine-Defay ratio

The most important characteristic of glass transition is a jump in the specific heat . Despite its significance, no standard theory exists to describe it. In this study, first-principles molecular-dynamics simulations are used to describe the glass transition of silica glass. The novel view that state variables are extended to include the equilibrium positions of atoms is fully used in analyzing the simulation results. Decomposing the internal energy into three components (structural, phonon, and thermal expansion energies) reveals that the jump of silica glass is entirely determined by the component of structural energy. The reason for the small is its high glass-transition temperature, which makes the fluctuation in the structural energy insensitive to the temperature change. This significantly affects how the Prigogine-Defay ratio Π is interpreted, which was previously unknown. The ratio Π represents the ratio of the total energy change to the contribution of thermal expansion energy at the glass transition. The general property, Π> 1, of glasses indicates that glass transitions occur mainly via the change in the structural energy. Silica glass is an extreme case in that the transition occurs entirely through the change in internal structure, such as the distribution of the bending angle of Si–O–Si bonds.

Changes in Admittance and Internal Structure of Coating Films by Environmental Testing

Changes in Admittance and Internal Structure of Coating Films by Environmental Testing

Drought Stress Tolerance in Vegetables: The Functional Role of Structural Features, Key Gene Pathways, and Exogenous Hormones.

The deleterious effects of drought stress have led to a significant decline in vegetable production, ultimately affecting food security. After sensing drought stress signals, vegetables prompt multifaceted response measures, eventually leading to changes in internal cell structure and external morphology. Among them, it is important to highlight that the changes, including changes in physiological metabolism, signal transduction, key genes, and hormone regulation, significantly influence drought stress tolerance in vegetables. This article elaborates on vegetable stress tolerance, focusing on structural adaptations, key genes, drought stress signaling transduction pathways, osmotic adjustments, and antioxidants. At the same time, the mechanisms of exogenous hormones such as abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) toward improving the adaptive drought tolerance of vegetables were also reviewed. These insights can enhance the understanding of vegetable drought tolerance, supporting vegetable tolerance enhancement by cultivation technology improvements under changing climatic conditions, which provides theoretical support and technical reference for innovative vegetable stress tolerance breeding and food security.

Rheology of Aqueous Ferrofluids: Transition from a Gel-Like Character to a Liquid Character in High Magnetic Fields

Rheological measurements under an applied magnetic field were used to investigate the changes to the internal structure and stability of an aqueous ferrofluid. The ferrofluid was prepared by dispersing 1.8 wt.% of maghemite nanoparticles with a size of d = 14 ± 3 nm and a saturation magnetization MS = 68 emu/g in water using citric acid as the surfactant. In this study, oscillatory tests were used to investigate the internal structural changes and the stability of ferrofluid under the influence of the magnetic field B. In a magnetic field of approximately 50 mT, the G′ became higher than the loss modulus G″ as the ferrofluid exhibited a gel-like character. However, at a magnetic field of approximately 200 mT, the character of the ferrofluid reverted to that of a liquid. The change in the character of the ferrofluid in this high magnetic field was associated with a gradual change from chain agglomerates to the energetically more favourable globular agglomerates, using a calculation based on a model described in a separate work. The globular agglomerates impeded the flow to a much lesser degree than the chains, causing a reduction in the viscosity. Further increase of the magnetic field resulted in sedimentation of agglomerates and loss of magneto-rheological effect.

Heat transfer enhancement for 3D chip thermal simulation and prediction

Parameter changes in the complex internal structure of multi-layer 3D stacked chips will greatly reduce the efficiency of modeling and thermal analysis. In this work, by combining thermal simulation analysis with machine learning algorithms, we can skillfully predict the hotspot temperature changes of 3D chips up to 28 layers while changing some key parameters of 3D chips with less computational effort. K-fold Cross Validation (K-CV) algorithm and support vector regression (SVR) algorithm were developed to predict the variations in hotspot temperature of the 3D chip while changing modeling and cooling parameters. Based on the training matrix, the support vector regression (SVR) model can accurately predict the random power distribution case, the random processor core location distribution case, and the random Through Silicon Via (TSV) distribution case. The validation results show that the prediction accuracy deviation is close to 0.6 K, and the correlation coefficient R2 is close to 1. Meanwhile, the variable parameter and variable layer prediction method based on the aforementioned training model can more accurately predict the hotspot temperature of the 3D chips with higher layers (28 layers) from the modeling analysis data of 3D chips with lower layers (4–5 layers). Its prediction deviation is less than 0.2%. The predicted data match the simulated numerical data quite well, indicating that the predictive algorithm can accurately feed the sample data set.

Strength and microstructure characteristics of red-bed weathered residual soil stabilized by Titanium Gypsum-Cement

Due to its unfavorable engineering characteristics, the red-bed weathered residual soil (RBRS) frequently necessitates remedial measures to conform to design specifications. Solidification with the aid of binders stands out as a prevalent technique employed to enhance the engineering attributes of RBRS. This study investigates the characteristics of RBRS stabilized with Titanium Gypsum-Cement (TGC) by conducting unconfined compressive strength tests on solidified soil with varying contents and curing times. Additionally, SEM and MIP tests were conducted to analyze the internal structure change, reaction mechanism, and hydration characteristics of the TGC-stabilized soil. The experimental findings revealed that the stress-strain response of specimens with varying proportions and curing times exhibited four distinct phases, namely the compaction stage, linear elastic deformation stage, pre-peak failure stage, and post-peak stage. Increasing cement content led to greater ductility in the stress-strain curve, while a higher ratio of TG to cement in the admixture resulted in a milder trajectory with a gradual decline in unconfined compressive strength. The stabilized soil with TGC ratio 6:4 (10%) and 9:6 (15%) met the compressive strength requirement at curing times of 7, 14, and 28 days. Microscopic investigations revealed the formation of hydration products, including CSH, with the incorporation of TGC. The resulting three-dimensional network effectively enveloped soil particles, enhancing inter-particle bonding and permeating mesopores and macropores, leading to soil structure compaction.

Study on Disintegration and Infiltration Characteristics of Nanjing Jiangxinzhou Embankment Soil

Flood season embankment due to long-term high water immersion environment creates situations where its soil properties will deteriorate, quickly triggering bank slope instability and other dangerous situations. In order to study embankment slope soil in the disintegration and permeability characteristics under the long-term water immersion process, disintegration tests and variable head infiltration tests were carried out on embankment-disturbed clays in Jiangxinzhou, Nanjing, Jiangsu Province, China. The results showed that the disintegration rate decreased with increasing dry density. Moreover, the samples gained weight quickly when the water content was low. Moreover, there is a threshold water content of around 20%, and it is assumed that the disintegration pattern of the soil may be significantly different depending on the optimum moisture content. The effect of moisture content on the disintegration of the samples was more significant. There were three main typical disintegration modes of the samples. The primary influencing factors resulting in the various disintegration patterns are the soil matrix suction, the change in consistency following water immersion, and the water membrane on the surface of the clay particles. The coefficient of permeability decreases with increasing dry density, and the variation of the coefficient of permeability with dry density coincides with the exponential function relationship. Long-term immersion conditions will change the permeability of the soil. An electron microscope test of immersed and unimmersed samples shows that the internal structure change is an essential factor affecting the permeability. After water immersion, with the dissolution of cementing material between soil particles, the particles move to fill the intergranular pore space so that the permeability coefficient decreases.

Anatomical observation and transcriptome analysis of branch-twisted mutations in Chinese jujube

BackgroundPlant organs grow in a certain direction and organ twisted growth, a rare and distinctive trait, is associated with internal structure changes and special genes. The twisted branch mutant of Chinese jujube jujube, an important fruit tree native to China and introduced to nearly 50 countries, provides new typical materials for exploration of plant twisted growth.ResultsIn this study, the cytological characteristics and related genes of twisted branches in Chinese jujube were revealed by microscopy observation and transcriptome analysis. The unique coexistence of primary and secondary structures appeared in the twisted parts of branches, and special structures such as collateral bundle, cortical bundles, and internal phloem were formed. Ninety differentially expressed genes of ‘Dongzao’ and its twisted mutant were observed, in which ZjTBL43, ZjFLA11, ZjFLA12 and ZjIQD1 were selected as candidate genes. ZjTBL43 was homologous to AtTBL43 in Arabidopsis, which was involved in the synthesis and deposition of cellular secondary wall cellulose. The attbl43 mutant showed significant inflorescence stem bending growth. The transgenic lines of attbl43 with overexpression of ZjTBL43 were phenotypically normal.The branch twisted growth may be caused by mutations in ZjTBL43 in Chinese jujube. AtIQD10, AtFLA11 and AtFLA12 were homologous to ZjIQD1, ZjFLA11 and ZjFLA12, respectively. However, the phenotype of their function defect mutants was normal.ConclusionIn summary, these findings will provide new insights into the plant organ twisted growth and a reference for investigation of controlling mechanisms of plant growth direction.

Micropore structure characterization of the bitumen emulsion-based cold in-place recycling mixture considering water gradient migration condition through multiple XCT scanning

Bitumen Emulsion-based Cold In-place Recycling (BE-CIR) has been widely used all around the world due to its superior environmental benefits. Unlike the hot mix asphalt (HMA), BE-CIR mixture presents a unique moisture migration behavior after compaction. The migration of water can alter the micro-pore structure of the BE-CIR mixture and then change its mechanical performance. Therefore, it is essential to characterize the variation of internal structure, especially the air voids, in depth direction during the curing process of BE-CIR mixture. A one-way evaporation method was developed to simulate the field moisture migration of BE-CIR mixture specimens in laboratory. To track the internal structure change with time, multiple X-ray Computed Tomography (XCT) scanning examinations were performed on the lab-prepared specimens with three different curing periods (0 h, 60 h, and 153 h). The effects of curing temperature and initial moisture content on the micropore structure development were also investigated. The morphology variations of air voids including the content, number, volume distribution and void gradation in the depth direction with curing time were further characterized. The results indicate that there is a 0.5% difference in air void content between the top and bottom of the BE-CIR mixture. The BE-CIR mixture's micropore structure evolved over the course of curing with two key characteristics: a fast increase in the number of small voids and a sustained development of large voids. The development of more tiny pores and the gradient properties of these pores, which predominated during the curing time of 60 to 153 h, were a reflection of the internal migration of free water. The initial moisture content mainly affects the magnitude of the large void variation during the curing time of 0 to 60 h, while the curing temperature affects the proportion of pore increase between the two curing periods. The outcomes can provide a better understanding on the dynamic volumetric characteristics of the BE-CIR pavement over the curing process.

A comprehensive investigation on sulphate resistance of geopolymer recycled concrete: Macro and micro properties

As a potential alternative to ordinary Portland cement concrete (OPC), geopolymer recycled concrete (GRC) is provided with great research value as it not only significantly reduces carbon dioxide (CO2) emissions but also promotes the resourceful use of construction and demolition waste (CDW). However, concrete is frequently subjected to extreme circumstances such as sulphate attack during service. To comprehensively explore the structural and performance damage mechanisms of GRC caused by sulphate assault, this study is conducted to erode GRC specimens with recycled aggregate (RA) replacement rate of 0%, 30%, 50%, 70% and 100% by using magnesium sulphate solution with 10% mass fraction. The macroscopic properties of GRC subjected to sulphate attack are examined, including mass loss rate, relative modulus of elasticity and compressive strength. The microstructure and phase evolution of GRC subjected to sulphate attack is analyzed by SEM, EDS and XRD. The micro-mechanical properties of interfacial transition zones (ITZs) in GRC are measured by nanoindentation. The results show that there is no loss of mass in all GRC specimens throughout the erosion age, and the relative dynamic elastic modulus of GRC first increases and then decreases. GRC with 30% RA substitution rate has the best resistance to sulphate attack. The degradation of GRC by sulphate attack is closely related to its internal structural changes. The deterioration of the micromechanical properties of three ITZs by sulphate erosion is not significant, which indicates the RA has favorable performances with geopolymer binder contributing to excellent resistance of GRC to sulfate attack. In addition, a prediction model for the evolution of GRC resistance to sulfate erosion with erosion age for different RA substitution rates is developed, which provides a theoretical guidance for the application of GRC.

An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell

Electrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microchannel for single-cell detection and electrical properties extraction. The dual microneedles utilized a commercially available tungsten needle coated with parylene. When a single cell flows through the parallel-facing electrode configuration of the dual microneedle, the electrical impedance at multiple frequencies is measured. The impedance measurement demonstrated the differential of normal red blood cells (RBCs) with three different sizes of microbeads at low and high frequencies, 100 kHz and 2 MHz, respectively. An electrical equivalent circuit model (ECM) was used to determine the unique membrane capacitance of individual cells. The proposed technique demonstrated that the specific membrane capacitance of an RBC is 9.42 mF/m−2, with the regression coefficients, at 0.9895. As a result, this device may potentially be used in developing countries for low-cost single-cell screening and detection.

Led-mediated instantaneous pore formation in membranes driven by structural transformation and deprotonation of phospholipids

The mechanisms by which molecules can be efficiently delivered directly into cells are of interest for fundamental cell biological research. Light-emitting diodes (LED) can be used to deliver substances into cells, but there is limited information on how LEDs alter the structure of the plasma membrane. In this study, we determined the mechanisms by which LED induces the instantaneous pore formation in the plasma membrane. We manufactured three types of artificial membrane (AM) composed of phosphatidylcholine (PC), phosphatidylethanolamine (PE), or a mixed form of binary PC/PE and investigated the surfaces and internal structural transformation and chemical changes caused by LED stimulation. LED irradiation (blue and near-infrared wavelengths) decreased the size of the three AMs due to the formation of pores on the surface and internal AM. PE and PC/PE AMs underwent LED-mediated structural transformation. In the PE AMs, chemical changes occurred through deprotonation of the ammonia cation (NH3+) located at the terminal of the head group of the phospholipid to an amine group (NH2). Furthermore, pore formation occurred in plasma membranes from cell lines and was used to deliver cell-impermeable molecules. Our study highlights a fundamental mechanism underlying pore formation at the molecular level.

Neutrinos and Asteroseismology of Stars over the Helium Flash

The helium flash, occurring in stars of 0.6–2.0 M ⊙ at the end of the red giant branch, is not observable via optical means due to the energy of the process being used to lift the core out of degeneracy. Neutrinos, which are linked to the ignition of reactions triggered during the flash and serve as the only cooling process in the inert core, can help characterize changes in internal structure. In this work, we create 18 stellar models across three mass and six metallicity values, chosen in the context of the stellar abundance problem, to compare the evolutionary path up to and probe the helium flash by conducting a detailed study of neutrino emission throughout this crucial phase of stellar evolution. We demonstrate how thermal neutrino emissions could have an imprint on global asteroseismic parameters and use them as an additional tool to infer the impact of compositional changes. We find that a precision of 0.3 μHz in the determination of Δν is enough to distinguish between between the two most prominent solar composition models and confirm that asteroseismic observation can be enough to classify a star as undergoing the process of helium subflashes. We also predict nuclear neutrino emission fluxes and their evolution for all relevant sources.

Effect of Anaerobic Calcium Oxide Alkalization on the Carbohydrate Molecular Structures, Chemical Profiles, and Ruminal Degradability of Rape Straw.

To improve the utilization efficiency of rape straw, anaerobic calcium oxide (CaO) alkalization was conducted, and advanced molecular spectroscopy was applied, to detect the internal molecular structural changes. Rape straw was treated with different combinations of CaO (3%, 5%, and 7%) and moisture levels (50% and 60%) and stored under anaerobic conditions. We investigated the carbohydrate chemical constituents, the ruminal neutral detergent fiber (aNDF) and acid detergent fiber (ADF) degradation kinetics, and the carbohydrate molecular structural features. CaO-treated groups were higher (p < 0.05) for ash, Ca, non-fiber carbohydrate, soluble fiber, and the ruminal degradability of aNDF and ADF. In contrast, they were lower (p < 0.05) for the contents of aNDF, ADF, and indigestible fiber. With CaO levels rising from 3% to 7%, the content of aNDF and ADF linearly decreased (p < 0.05). CaO treatment and anaerobic storage changed the molecular characteristics, including structural parameters related to total carbohydrates (TC), cellulosic compounds (CEC), and structural carbohydrates (STC). Alterations in cellulosic compounds' spectral regions were highly correlated with the differences in carbohydrate chemical constituents and the ruminal digestibility of rape straw. In summary, CaO treatment and anaerobic storage altered the molecular structural parameters of carbohydrates, leading to an enhancement in the effective degradability (ED) of aNDF and ADF in rape straw. From the perspective of processing cost and effectiveness, 5% CaO + 60% moisture could be suggested as a recommended treatment combination.