An important application for training instructions is found in directing one's attentional focus. This direction can occur in different internal, external, or holistic forms. However, comparison between these three forms of instructions is a relatively recent development rarely reported at different skill levels or various sports including karate. Therefore, the present study attempts to investigate the effects of three forms of instructions on standing long jump performance in skilled and novice karatekas. The participants were 60 skilled and novice karatekas (all females; mean age: 21.32±1.65) who completed 12 standing long jump trials under different focus conditions (3 trials for each condition: internal, external, holistic and control condition) in a counterbalanced order. Our findings suggested significant main effects, indicating that skilled karatekas outperformed the novices. The findings also showed that regardless of skill levels, the participants exhibited similar performance under external and holistic focus conditions while performance in both of these conditions was superior compared to performance under internal focus and control conditions. No difference was observed between the control and internal focus conditions. It seems that skilled and novice karatekas may benefit from holistic and external focus of attention instructions which enhance their motor performance. Thus, it is recommended that coaches should incorporate these two types of attentional focus instructions into their training sessions.