Exercise intensity is considered to be a key factor affecting the release of cardiac troponin (cTn). However, most of the studies focus on responses of endurance sports or exercise. The experimental data of cTn and other biomarkers responses to intermittent exercise is still limited. PURPOSE: To determine the correlations between plasma cardiac troponin I (cTnI) and blood lactate, body mass index (BMI), as well as physiological characteristics after intermittent running. METHODS: Eighteen healthy, physically active collegiate males performed a graded exercise test to determine their VO2max and completed the intermittent running protocol 48 h later. This exercise protocol consisted of six 4-min running bouts and separated by 3 min of rest. Running velocity was set at an average of 90% VO2max. Capillary blood samples were drawn before and immediately after exercise for measuring lactate. Venous blood samples were collected at pre- and 3 h post-exercise for measuring cTnI. The nonparametric Spearman’s rank order correlation coefficient was used for statistic analysis. RESULTS: Plasma cTnI concentration was significantly increased following exercise. The delta cTnI was positively correlated with delta lactate (p = .013, rho = .57). However, no significant correlations were found between cTnI and body mass index, VO2max, as well as running velocity. CONCLUSION: The intermittent running protocol of this study induced the elevation in cTnI. The release of cTnI was associated with blood lactate level. As endurance sports, the intensity of intermittent exercise might be a factor affecting the release of cTn.