Intermediate Frequency Magnetic Fields Research Articles

Development of an <i>in vivo</i> pain assessment method for exposure to intermediate-frequency magnetic fields

Development of an <i>in vivo</i> pain assessment method for exposure to intermediate-frequency magnetic fields

In vivo genotoxicity of high-intensity intermediate frequency magnetic fields in somatic cells and germ cells.

Intermediate frequency magnetic fields (IF-MFs) at ~85kHz are one of the components of wireless power transfer (WPT) systems. However, the available data needed for the assessment of the safety of organisms from IF-MF exposure are scarce. Thus, there is an imminent need to accumulate evidence-based assessment data. In particular, if humans are exposed to IF-MF due to an accident or trouble, they are at increased risk of being exposed to high-intensity IF-MF within a short period. The already existing exposure system was improved to a system that could intermittently expose animals at 3s intervals. This system allows the exposure of a mouse to high-intensity IF-MF (frequency: 82.3kHz; induced electric field: 87V/m, which was 3.8 times the basic restriction level for occupational exposure in the ICNIRP guideline), while regulating the heat generated by the coil. In vivo genotoxicity after IF-MF exposure was assessed using micronucleus (MN) test, Pig-a assay, and gpt assay. The results of MN test and Pig-a assay in hematopoietic cells revealed that neither the reticulocytes nor the mature erythrocytes exhibited significant increases in the IF-MF-exposed group compared with that in the sham-exposed group. In germ cells, MN test and gpt assay outcomes showed that IF-MF exposure did not cause any genetic or chromosomal abnormality. Based on these data, there was no genotoxic effect of our set IF-MF exposure on somatic and germ cells. These findings can contribute to the widespread use of WPT systems as effective data of IF-MF safety assessment.

Measurement of Intermediate Frequency Magnetic Fields Generated by Household Induction Cookers for Epidemiological Studies and Development of an Exposure Estimation Model.

Introduction: Exposure assessment of intermediate frequency (IF) electromagnetic fields (EMFs) is difficult and epidemiological studies are limited. In the present study, we aimed to estimate the exposure of pregnant women to IF-EMFs generated by induction cookers in the household using a questionnaire and discussed its applicability to epidemiological studies. Method: Two main home-visit surveys were conducted: a Phase 1 survey to develop an estimation model and a Phase 2 survey to validate the model. The estimation model included the following variables: wattage, cookware diameter, and distance from the hob center (center of the stove). Four models were constructed to determine the importance of each variable and the general applicability for epidemiological studies. In addition, estimated exposure values were calculated based on the Phase 2 survey questionnaire responses and compared with the actual measured values using the Spearman rank correlation coefficient. Result: The average value of the magnetic field measured in the Phase 1 survey was 0.23 μT (variance: 0.13) at a horizontal distance of 30 cm at the height of the cooking table. The highest validity model was inputted distance from the hob center to the body surface that is variable (correlation coefficient = 0.54, 95% confidence interval: 0.22–0.75). No clear differences were identified in the correlation coefficients for each model (z-value: 0.09–0.18, p-value: 0.86–0.93). Discussion and Conclusions: No differences were found in the validity of the four models. This could be due to the biased wattage of the validation population, and for versatility it would be preferable to use three variables (distance, wattage, and estimation using the diameter of the cookware) whenever possible. To our knowledge, this is the first systematic measurement of magnetic fields generated by more than 70 induction cookers in a real household environment. This study will contribute to finding dose–response relationships in epidemiological studies of intermediate-frequency exposure without the use of instrumentation. One of the limitations of this study is it estimates instantaneous exposure in place during cooking only.

Measurement and Exposure Assessment of Intermediate Frequency Magnetic Fields From Electronic Article Surveillance (EAS) Gates in Libraries.

Exposure to magnetic fields from the electronic article surveillance (EAS) gate was evaluated in consideration of the application to epidemiological studies of library workers who are exposed continually to intermediate frequency magnetic fields from the EAS gate. Two types of exposures were investigated. One was transient exposure due to passing through or beside the gate and another was chronic exposure in the room. We measured magnetic fields from five EAS gate models which were commonly used in libraries in Japan. Detailed measurements were performed for two of them in consideration of the phase difference of vector components of magnetic flux density. The polarization of the magnetic field in the gate was investigated with the index of ellipticity. The induced electric field in a human body was numerically calculated for exposures to magnetic fields of the two gate models. The results provide a quantitative understanding of exposures during passing through or by the EAS gate. Magnetic field distribution was measured in a large room for one gate model to quantify the chronic exposure of library workers during the work at the desk. It was found that the magnetic field was distributed as a function of the horizontal distance to the nearest gatepost. The 45-point average value BIEC defined by the IEC standard was suggested to be a useful quantity to characterize the magnitude of the magnetic field from the EAS gate. Exposures to different EAS gates are expected to be compared through this quantity without detailed measurements. These results are expected to provide useful means for exposure assessment of epidemiological studies on the association between the IF-EMF exposure and possible health outcomes.

Measurement of Intermediate Frequency Magnetic Fields Generated by Household Induction Cookers for Epidemiological Studies and Development of an Exposure Estimation Model

Measurement of Intermediate Frequency Magnetic Fields Generated by Household Induction Cookers for Epidemiological Studies and Development of an Exposure Estimation Model

Investigation of Genotoxicity Induced by Intermediate Frequency Magnetic Field Combined with Ionizing Radiation: An in Vitro Study on Human Fibroblast Cells

Investigation of Genotoxicity Induced by Intermediate Frequency Magnetic Field Combined with Ionizing Radiation: An in Vitro Study on Human Fibroblast Cells

Effects of Long-Term Exposure of Intermediate Frequency Magnetic Fields (20 kHz, 360 µT) on the Development, Pathological Findings, and Behavior of Female Mice.

The use of magnetic fields in the intermediate-frequency (IF) range to wirelessly charge electric cars with power transfer in the kilowatt range has become increasingly widespread, leading to unavoidable stray fields in the microtesla range. Only a handful of studies have assessed the potential biological risks associated with exposure to such fields. We exposed female mice (n = 80 per group) to either 20 kHz, 360 µT (rms), or sham in Helmholtz coils to conduct a blind design study. Exposure started at 3 months of age (24 h/day). Body mass was recorded every 1-2 weeks. At 10 months of age, three behavioral tests were performed on 24 animals per group. Three months later, the mice were sacrificed and organs (brain, liver, kidney, spleen, and lung) were removed and prepared for microscopic analysis. Our findings demonstrate no differences in the development of body mass and survival rates (96% and 89%, respectively). Similarly, no significant differences were observed in tumor incidence rates. When it comes to behavioral tests, the 8-arm maze results revealed no significant differences. In contrast, the Rotarod data were significantly (P < 0.001) different with longer retention times seen in the exposed mice. In the open field, the number of supported rears was significantly lower (P < 0.01), whereas the other endpoints did not show any differences. Overall, our data reveal no adverse effects of exposure to 20 kHz, 360 µTon the development and tumor incidences, while the significant differences in the behavioral tests may indicate higher levels of alertness in mice.

No evidence for genotoxicity in mice due to exposure to intermediate-frequency magnetic fields used for wireless power-transfer systems

Time varying magnetic fields (MFs) are used for the wireless power-transfer (WPT) technology. Especially, 85 kHz band MFs, which are included in the intermediate frequency (IF) band (300 Hz – 10 MHz), are commonly used WPT system for charging electric vehicles. Those applications of WPT technology have elicited public concern about health effects of IF-MF. However, existing data from health risk assessments are insufficient and additional data are needed. We assessed the genotoxic effects of IF-MF exposure on erythroid differentiation in mice. A high-intensity IF-MF mouse exposure system was constructed to induce an average whole-body electric field of 54.1 V/m. Blood samples were obtained from male mice before and after a 2-week IF-MF exposure (1 h/day, total: 10 h); X-irradiated mice were used as positive controls. We analyzed the blood samples with the micronucleus (MN) test and the Pig-a mutation assay. No significant differences were seen between IF-MF-exposed and sham-exposed mice in the frequencies of either MN or Pig-a mutations in mature erythrocytes and reticulocytes. IF-MF exposure did not induce genotoxicity in vivo under the study conditions (2.36× the basic restriction for occupational exposure, 22.9 V/m, in the International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines). The absence of significant biological effects due to IF-MF exposure supports the practical application of this technology.

The response of the neuronal activity in the somatosensory cortex after high-intensity intermediate-frequency magnetic field exposure to the spinal cord in rats under anesthesia and waking states

Novel technologies using the intermediate-frequency magnetic field (IF-MF) in living environments are becoming popular with the advance in electricity utilization. However, the biological effects induced by the high-intensity and burst-type IF-MF exposure used in the wireless power transfer technologies for electric vehicles or medical devices, such as the magnetic stimulation techniques, are not well understood. Here, we developed an experimental platform using rats, that combined an 18 kHz, high-intensity (Max. 88 mT), Gaussian-shaped burst IF-MF exposure system with an in vivo extracellular recording system. In this paper, we aimed to report the qualitative differences in stimulus responses in the regions of the somatosensory cortex and peripheral nerve fibers that were induced by the IF-MF exposure to the rat spinal cord. We also report the modulation of the stimulus responses in the somatosensory cortex under anesthesia or waking states. Using this experimental platform, we succeeded in the detection of the motor evoked potentials or the neuronal activity in the somatosensory cortex that was induced by the IF-MF exposure to the spinal cord in rats. Compared to the state of anesthesia, the neuronal activities in the somatosensory cortex was enhanced during the waking state. On the other hand, these neuronal responses could not be confirmed by the IF-MF exposure-related coil sound only. Our experimental results indicated the basic knowledge of the biological responses and excitation mechanisms of the spinal cord stimulation by the IF-MF exposure.

Intermediate frequency magnetic field at 250.8 kHz does not induce DNA damage or “Adaptive Response” in vitro

The aim of this study was to examine whether intermediate frequency (IF)magnetic field (MF) induces genotoxic effects or play a role in the induction ofadaptive response after combined exposure to IF MF and ionizing radiation inleukocytes and in adrenocortical carcinoma cell line H295R. Cells were exposedto 250.8 kHz at the magnetic field strength of 80 A/m (equivalent to 100 µTmagnetic flux density) for 20 hours, or exposed to IF MF for 20 hours and 24hours later challenged with ionizing radiation (1.5 Gy X-ray). Evaluation of theDNA damage was performed with alkaline comet assay. Our results showed thatthere was no significant genotoxic effect of IF MF exposure compared to thecontrols in both cell types. Furthermore, results did not indicate a statisticallysignificant change in DNA strand breaks in IF MF pre-exposed cells when theywere subsequently exposed to 1.5 Gy. Consequently no adaptive response wasdetected.

Genotoxic effects of intermediate frequency magnetic fields on blood leukocytes in vitro

The widespread presence of electromagnetic sources in daily life has initiated several studies on the effects of radiofrequency and power frequency fields. Only few investigations on the genotoxic effects of exposure to intermediate frequency magnetic fields (IF-MF) have been done so far. Therefore, the aim of this study was to evaluate possible genotoxic effects of exposure to 123.90 kHz and 250.80 kHz IF-MF on canine and human blood. Blood was exposed to IF-MF at 630 A/m (0.79 mT) and 80 A/m (0.10 m T) with exposure durations of 1–5 h (hourly), 20 and 24 h. Cylindrically divided Petri dish system was developed for in vitro exposures where different induced current could be achieved in the samples at the same magnetic flux density level. For the assessment of genotoxicity the alkaline comet assay was applied. We detected a statistically significant increase in DNA damage only following 20 h exposure to IF-MF.

Global Analysis of Transcriptional Expression in Mice Exposed to Intermediate Frequency Magnetic Fields Utilized for Wireless Power Transfer Systems.

Background: Intermediate frequency magnetic fields (IF-MFs) at around 85 kHz are a component of wireless power transfer systems used for charging electrical vehicles. However, limited data exist on the potential health effects of IF-MFs. We performed a comprehensive analysis of transcriptional expression in mice after IF-MF exposure. Materials and Methods: We developed an IF-MF exposure system to generate a high magnetic flux density (25.3 mT). The system can expose the IF-MF for a mouse whole-body without considering thermal effects. After 10 days (1 h/day) of exposure, a comprehensive expression analysis was performed using microarray data from both the brain and liver. Results: No significant differences in transcriptional expression were detected in the 35,240 probe-sets when controlling the false discovery rate (FDR) under a fold change cutoff >1.5. However, several differential expressions were detected without FDR-adjustment, but these were not confirmed by RT-PCR analysis. Conclusions: To our knowledge, this is the first in vivo study to evaluate the biological effects of IF-MF exposure with an intense magnetic flux density 253 times higher than the occupational restriction level defined by the International Commission on Non-Ionizing Radiation Protection guidelines. However, our findings indicate that transcriptional responses in the living body are not affected under these conditions.

Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields

We investigated whether exposure to intermediate frequency magnetic fields (IF MFs) could induce or enhance genomic instability in primary astrocytes. Rat primary astrocytes were exposed to vertical or horizontal 7.5 kHz, 300 μT MF for 24 h. To study possible combined effects with known genotoxic agents, the cells were exposed for 3 h to menadione or methyl methanesulfonate after the MF treatment. Induced genomic instability was evaluated 36 days after exposures using the Comet assay and flow cytometric scoring of micronuclei. Exposure to 7.5 kHz, 300 μT MF did not induce genomic instability alone or in combination with chemicals in measurements performed several cell generations after exposure.

Carcinogenicity of intermediate frequency magnetic field in Tg.rasH2 mice.

Although the likelihood of exposure to leaking intermediate frequency magnetic fields (MFs) from electronic devices, such as induction‐heating and wireless power transfer systems, has increased, biological data assessing the health risks associated with human exposure remain insufficient. We examined the carcinogenicity of a 20 kHz MF, a typical frequency produced by induction‐heating cookers, using a transgenic rasH2 mouse model. Twenty‐five male and female CByB6F1‐Tg(HRAS)2Jic mice were exposed to a 0.20 mT, 20 kHz MF (22 h/day) or sham‐exposed for 26 weeks. As a positive control, 10 male and female rasH2 mice from the same batch were administered a single intraperitoneal injection of 75 mg/kg N‐methyl‐N‐nitrosourea. A blinded histopathological evaluation was performed, and the same experiments were conducted twice, independently, to confirm the reproducibility of the results. Histopathological examination revealed that spontaneous neoplastic lesions, such as splenic hemangiosarcomas and gastric squamous cell papillomas, were less (1–3 per group) in the MF‐ and sham‐exposed groups. The frequency of the neoplastic lesions was not significantly different between the groups. Eight to ten mice in each positive‐control group exhibited malignant lymphoma. The outcomes were consistent between duplicated experiments, which indicates lack of carcinogenicity of 20 kHz MF in the rasH2 mouse model. Bioelectromagnetics. © 2019 The Authors. Bioelectromagnetics Published by Wiley Periodicals, Inc.

Genotoxicity of intermediate frequency magnetic fields in vitro and in vivo

We assessed genotoxic effects of intermediate frequency magnetic fields (MF) in vitro and in vivo. Rat primary astrocytes were exposed for 24 h to a 7.5 kHz MF at a magnetic flux density of 30 or 300 µT. Male C57BL/6 J mice were exposed continuously for 5 weeks to a 7.5 kHz MF at 12 or 120 μT, and blood samples were collected for the genotoxicity assays. To evaluate possible co-genotoxicity, the in vitro experiments included combined exposure with menadione (an agent that induces mitochondrial superoxide production and DNA damage) and methyl methanesulfonate (an alkylating agent). DNA damage and DNA repair (in vitro) were measured using the alkaline Comet assay and formation of micronuclei was assessed microscopically (in vivo) or using flow cytometry (in vitro). The results did not support genotoxicity or co-genotoxicity of 7.5 kHz MFs at magnetic flux densities up to 300 µT in vitro or in vivo. On the contrary, there was some evidence that exposure to 7.5 kHz MFs might reduce the level of genetic damage. Strongest indication of any biological effects was obtained from measurements of relative cell number, which was significantly and consistently increased after MF exposure in all in vitro experiments. Health implications of this finding are unclear, but it suggests that 7.5 kHz MFs may stimulate cell proliferation or suppress cell death.

A pilot study on the reproductive risks of maternal exposure to magnetic fields from electronic article surveillance systems

Purpose: We investigated the feasibility of a large-scale epidemiological study on reproductive effects of intermediate frequency (IF) magnetic field (MF) exposure among cashiers working near electronic article surveillance (EAS) systems.Materials and methods: The study cohort included 4157 women who had worked as cashiers in supermarkets with EAS devices (considered as exposed) or grocery stores without EAS devices (considered as unexposed) between 2008 and 2015. 536 births and 38 miscarriages occurred among these women during the study period, based on information from nationwide health registries. Measurements were also performed to characterize the MF exposure of cashiers.Results: Cashiers were found to be exposed to 8.2 MHz MFs only when passing by the gates at short distance. Static fields of about 0.1 mT were observed at cashier’s seat. Extremely low frequency MFs were higher at stores without EAS devices. No differences on the risk of miscarriage, reduced birth weight or preterm birth were observed between cashiers in different store types.Conclusions: Any further studies should attempt to include study subjects working near EAS systems that produce stronger IF MFs at kHz frequencies. Exposure to ELF MFs should be assessed as a possible confounding factor.

In silico modelling of influence from low or intermediate frequency magnetic fields on users of wearable insulin pumps

Purpose: The aim was to model the effects of exposure to a low or intermediate frequency electromagnetic field (LIF-EMF), characterized by the electric field induced in the body, in order to evaluate how the type of insulin needle and the way it is injected influences the exposed user of a wearable insulin pump.Material and methods: Numerical models of exposure scenarios (sources of LIF-EMF, with a dominant magnetic component: 50Hz–1MHz; the insulin needle type; the way it is injected and insulin pump user) were worked out. The influence of the insulin needle on the user’s safety (the ratio of the induced electric field in tissues surrounding the needle and tissues of a person without an injection, both exposed identically) were calculated.Results: The effects of LIF-EMF exposure in insulin pump users were found to be up to approximately 7-times higher, varying with statistically significance (p < .05) with the material of the needle, the way it is injected and the polarization of the affecting magnetic field.Conclusions: When steel insulin needles is used, the assessment of user’s EMF exposure should be carried out using magnetic field limits at least 5-times lower than given in general international requirements.

Real-time detection of stimulus response in cultured neurons by high-intensity intermediate-frequency magnetic field exposure.

Threshold values of neuronal stimulation and modulation associated with exposure to time-varying electromagnetic fields contribute to establishing human protection guidelines and standards. However, biological evidence of threshold values in the intermediate-frequency range is limited. Additionally, although it is known that dendrites, a type of unmyelinated neuronal fibre, play an important role in information processing in the central nervous system, the stimulus threshold in dendrites has not been sufficiently investigated. We evaluated the excitation site-specific stimulus response of rat brain-derived cultured neurons by using a 20 kHz high-intensity intermediate-frequency magnetic field (hIF-MF) exposure system, a non-conductive fibre-optic imaging (NCFI) system, combined with a micro-patterning technique. Our hIF-MF exposure and NCFI system permitted real-time detection of the intracellular calcium ([Ca2+]i) spikes in neuronal cell bodies or unmyelinated neuronal fibres during exposure to a 20 kHz, 70 mT (peak), burst-type sinusoidal wave hIF-MF. Dosimetry of the induced electric fields intensities in the extracellular solution indicated that about 50% of unmyelinated neuronal fibres respond at about 147 V m-1. In contrast, the threshold of the [Ca2+]i spikes in neuronal cell bodies were lower than that in unmyelinated neuronal fibres. Our results provide a basis for understanding site-specific differences in the responses of cultured neurons to hIF-MFs.

Behavioural phenotypes in mice after prenatal and early postnatal exposure to intermediate frequency magnetic fields

Electromagnetic fields are ubiquitous in the environment. Human exposure to intermediate frequency (IF) fields is increasing due to applications like electronic article surveillance systems, wireless power transfer, and induction heating cooking hobs. However, there are limited data on possible health effects of exposure to IF magnetic fields (MF). In the present study, we set out to assess cognitive and behavioural effects of IF MF in mice exposed during prenatal and early postnatal periods. Pregnant female mice were exposed continuously to 7.5kHz MFs at 12 and 120μT, from mating until weaning of pups. Sham exposed pregnant mice were used as a control group. A behavioural teratology study was conducted on the male offspring at two months of age to detect possible effects on the developing nervous system. Body weight development did not differ between the exposure groups. The exposure did not alter spontaneous motor activity when exploring a novel cage or anxiety in novelty-suppressed feeding or marble burying tests. Improved performance in the Rotarod task was observed in the 12µT group, while the 120μT exposure group swam more slowly than the sham exposed group in the Morris swim navigation task. However, indices of learning and memory (path length and escape latency during task acquisition and search bias during the probe test) did not differ between the exposure groups. Furthermore, the passive avoidance task did not indicate any impairment of long-term memory over a 48h interval in the exposed groups. In a post-mortem histopathological analysis, there was no evidence for an effect of IF MF exposure on astroglial reactivity or hippocampal neurogenesis. The results suggest that the IF MF used did not have detrimental effects on spatial learning and memory or histological markers of tissue reaction. The two statistically significant findings that were observed (improved performance in the Rotarod task in the 12µT group and decreased swimming speed in the 120µT group) are likely to be chance findings, as they do not form an internally consistent, dose-dependent pattern indicative of specific developmental effects.

Behavioral testing of mice exposed to intermediate frequency magnetic fields indicates mild memory impairment.

Human exposure to intermediate frequency magnetic fields (MF) is increasing due to applications like electronic article surveillance systems and induction heating cooking hobs. However, limited data is available on their possible health effects. The present study assessed behavioral and histopathological consequences of exposing mice to 7.5 kHz MF at 12 or 120 μT for 5 weeks. No effects were observed on body weight, spontaneous activity, motor coordination, level of anxiety or aggression. In the Morris swim task, mice in the 120 μT group showed less steep learning curve than the other groups, but did not differ from controls in their search bias in the probe test. The passive avoidance task indicated a clear impairment of memory over 48 h in the 120 μT group. No effects on astroglial activation or neurogenesis were observed in the hippocampus. The mRNA expression of brain-derived neurotrophic factor did not change but expression of the proinflammatory cytokine tumor necrosis factor alpha mRNA was significantly increased in the 120 μT group. These findings suggest that 7.5 kHz MF exposure may lead to mild learning and memory impairment, possibly through an inflammatory reaction in the hippocampus.