Interface Conditions Research Articles

Mendelian segregation and high recombination rates facilitate genetic analyses in Cryptosporidium parvum.

Very little is known about the process of meiosis in the apicomplexan parasite Cryptosporidium despite the essentiality of sex in its life cycle. Most cell lines only support asexual growth of Cryptosporidium parvum (C. parvum), but stem cell derived intestinal epithelial cells grown under air-liquid interface (ALI) conditions support the sexual cycle. To examine chromosomal dynamics during meiosis in C. parvum, we generated two transgenic lines of parasites that were fluorescently tagged with mCherry or GFP on chromosomes 1 or 5, respectively. Infection of ALI cultures or Ifngr1-/- mice with mCherry and GFP parasites resulted in cross-fertilization and the formation of "yellow" oocysts, which contain 4 haploid sporozoites that are the product of meiosis. Recombinant oocysts from the F1 generation were purified and used to infect HCT-8 cultures, and phenotypes of the progeny were observed by microscopy. All possible phenotypes predicted by independent segregation were represented equally (~25%) in the population, indicating that C. parvum chromosomes exhibit a Mendelian inheritance pattern. The most common pattern observed from the outgrowth of single oocysts included all possible parental and recombinant phenotypes derived from a single meiotic event, suggesting a high rate of crossover. To estimate the frequency of crossover, additional loci on chromosomes 1 and 5 were tagged and used to monitor intrachromosomal crosses in Ifngr1-/- mice. Both chromosomes showed a high frequency of crossover compared to other apicomplexans with map distances (i.e., 1% recombination) of 3-12 kb. Overall, a high recombination rate may explain many unique characteristics observed in Cryptosporidium spp. such as high rates of speciation, wide variation in host range, and rapid evolution of host-specific virulence factors.

Intrinsic Elastomer with Remarkable Dielectric Constant via Elastification of Relaxor Ferroelectric Polymer.

High-dielectric-constant elastomers always play a critical role in the development of wearable electronics for actuation, energy storage, and sensing; therefore, there is an urgent need for effective strategies to enhance dielectric constants. The present methods mainly involve adding inorganic or conductive fillers to the polymer elastomers, however, the addition of fillers causes a series of problems, such as large dielectric loss, increased modulus, and deteriorating interface conditions. Here, the elastification of relaxor ferroelectric polymers is investigated through slight cross-linking, aiming to obtain intrinsic elastomers with high-dielectric constants. By cross-linking of the relaxor ferroelectric polymer poly(vinylidene fluoride-ter-trifluoroethylene-ter-chlorofluoroethylene) with a long soft chain cross-linker, a relaxor ferroelectric elastomer with an enhanced dielectric constant is obtained, twice that of the pristine relaxor ferroelectric polymer and surpassing all reported intrinsic elastomers. This elastomer maintains its high-dielectric constant over a wide temperature range and exhibits robust mechanical fatigue resistance, chemical stability, and thermal stability. Moreover, the ferroelectricity of the elastomer remains stable under strains up to 80%. This study offers a simple and effective way to enhance the dielectric constant of intrinsic elastomers, thus facilitating advancements in soft robots, biosensors, and wearable electronics.

A reaction-diffusion model for population dynamics in patchy landscapes

We address the linear eigenvalue problem associated with reaction-diffusion models for patchy linear domains with any finite number of unique patches. Interface conditions between adjacent patches may be chosen independently to accurately reflect organism behavior. We show that there exists a real principal eigenvalue with a corresponding non-negative eigenfunction that is non-zero interior to the domain. We demonstrate that our model is a generalization of several existing two-patch models. We consider simple three- and four-patch landscapes representing fragmented reserves to illustrate how our model can be used to analyze population persistence, spatial distribution, and migration dynamics. Fragmented landscapes with increased heterogeneity capture migration dynamics not found in landscapes with only two patch types, such as favorable habitat patches that experience net immigration. Conditions for the existence and non-existence of zero-flux conditions interior to three-patch systems are shown, which provide insight to the location of sources and direction(s) of migration.

Relaxed and Bonded Interfacial Conditions for the Axial Interaction of a Flexible Plate with a Transversely Isotropic Half-Space

Relaxed and Bonded Interfacial Conditions for the Axial Interaction of a Flexible Plate with a Transversely Isotropic Half-Space

Passivation of 1D@3D perovskite ferroelectric film with hydrophobic molecules for ferro-pyro-phototronic effect-based self-driven photodetector

High-performance, self-driven photodetectors in commercial and public applications show promising prospects. The pyro-phototronic effect is a promising method for building these detectors, but limitations in interfacial contact conditions hinder the use of the ferro-pyro-phototronic effect. By modifying the surface of 1D@3D perovskite ferroelectric film with tetra-ethyl ammonium (TEAI) molecules, the interfacial defect density is reduced, resulting in a high-performance, stable photodetector. Moreover, the passivation can greatly enhance the ferro-pyro-phototronic effect, which can be explained by the increased band bending and decreased trap states at the SnS/perovskite interface resulting in less re-distribution of charge carriers directly across the interface. Our work offers a feasible and effective method for producing pyro-phototronic responses in perovskite films-based devices, and thus presents a feasible solution for high-performance, self-driven and flexible photodetection.

Equivalent analytical model for liquid sloshing in a 2-D rectangular container with multiple vertical baffles by subdomain partition approach

An equivalent analytical model of sloshing in a two-dimensional (2-D) rigid rectangular container equipped with multiple vertical baffles is presented. Firstly, according to the subdomain partition approach, the total liquid domain is partitioned into subdomains with the pure interface and boundary conditions. The separation of variables is utilized to achieve the velocity potential for subdomains. Then, sloshing characteristics are solved according to continuity and free surface conditions. According to the mode orthogonality of sloshing, the governing motion equation for sloshing under horizontal excitation is given by introducing generalized time coordinates. Besides, by producing the same hydrodynamic shear and overturning moment as those from the original container-liquid-baffle system, a mass-spring analytical model of the continuous liquid sloshing is established. The equivalent masses and corresponding locations are presented in the model. The feasibility of the present approach is verified by conducting comparative investigations. Finally, by utilizing normalized equivalent model parameters, the sloshing behaviors of the baffled container are investigated regarding baffle positions and heights as well as the liquid height, respectively.

Homogenized moduli and local multiphysics fields of unidirectional piezoelectric nanocomposites with energetic surfaces

The study extends the locally-exact homogenization theory (LEHT) to determine the effective parameters and localized multiphysics fields of unidirectional piezoelectric nanocomposites with energetic surfaces. The interface is simulated using the generalized Gurtin-Murdoch model for piezoelectric nanocomposites, taking into account the discontinuities between surface stresses and electrical displacement. By characterizing hexagonal and square repeating unit cells (RUC), the interactions across a fiber's or pore's interface in piezoelectric nanocomposites with energetic surfaces have been analyzed. The extended LEHT possesses the advantages of fast convergence, excellent stability, and computational efficiency due to its avoidance of mesh discretization and pointwise satisfaction of interfacial conditions. To verify the validity of the present theory, this paper also derives the Eshelby solution for piezoelectric nanocomposite with energetic surfaces. The reliability and accuracy of the present theory are demonstrated by comparing the present results from the Eshelby solution and the finite element method (FEM) with generally good agreement. On this basis, the influence of microstructural effects, such as phase volume fractions and geometrical arrangement, on the effective and local responses of piezoelectric nanocomposites with energetic surfaces are investigated. The results show that effective piezoelectric/dielectric moduli and electric displacement field for piezoelectric nanocomposites are significantly affected by the size effect, along with several other parameters such as unit cell arrays and fiber/pore volume fractions. Those results highlight the significance of neighboring pore or fiber interactions for piezoelectric nanocomposites, which are often overlooked in traditional micromechanics models and are challenging to be captured using numerical methods.

Numerical Investigation of Evaluating the Degree of Damage in Concrete Plate Cold Joints through Surface Wave Dispersion Velocity Profiles

This study aims to develop a technology for assessing the interfacial condition of cold joints in concrete plates, focusing on the dispersion velocity profile of surface waves. The methodology employs a small impact hammer embedded with a piezoelectric element and a displacement receiver strategically positioned on either side of the seam. In this initial investigation, numerical models were constructed using ANSYS LS-DYNA. The cold joint zone was simulated by randomly removing elements with a predetermined void ratio. Displacement waveforms at the sensor node underwent analysis using a short-time Fourier Transform and the reassigned method to derive the dispersive velocity profile of surface waves. The study delves into the impacts of varying test-line lengths, impact durations, and void distributions. Numerical findings indicate that cold joints are detectable with a void ratio of 40% or greater. For the 60% and 80% void ratios, surface wave velocities register notably lower for wavelengths below 0.1 m and 0.2 m, respectively. Under the same porosity ratio for surface-observable cold joints, if their extension depth is smaller, the wave velocity on the dispersion curve will be lower than that of a fully penetrated cold joint. The current study reveals that a contact duration of 60 μs and an impactor-receiver distance of 0.82 m yield the most distinguishable results for detecting the extension and porosity ratio of a cold joint. Accompanied by tests with a hammer with a contact duration of 20 μs, additional information about the joint can be unveiled. Finally, various void distributions in the cold joint predominantly influence the wave velocity at shorter wavelengths.

First-principles-based machine learning interatomic potential for molecular dynamics simulations of 2D lateral MoS2/WS2 heterostructures

Understanding the mechanical and thermodynamic properties of transition-metal dichalcogenides (TMDs) and their heterostructures is pivotal for advancing the development of flexible semiconductor devices, and molecular dynamics (MD) simulation is widely applied to study these properties. However, current uncertainties persist regarding the efficacy of empirical potentials in MD simulations to accurately describe the intricate performance of complex interfaces within heterostructures. This study addresses these challenges by developing an interatomic potential based on deep neural networks and first-principles calculations. Specifically focusing on MoS2/WS2 heterostructures, our approach aims to predict Young's modulus and thermal conductivities. The potential's effectiveness is demonstrated through the validation of structural features, mechanical properties, and thermodynamic characteristics, revealing close alignment with values derived from first-principles calculations. A noteworthy finding is the substantial influence of the load direction on Young's modulus of heterostructures. Furthermore, our results highlight that the interfacial thermal conductance of the MoS2/WS2 heterostructures is considerably larger than that of graphene-based interfaces. The potential developed in this work facilitates large-scale material simulations, bridging the gap with first-principles calculations. Notably, it outperforms empirical potentials under interface conditions, establishing its significant competitiveness in simulation computations. Our approach not only contributes to a deeper understanding of TMDs and heterostructures but also presents a robust tool for the simulation of their mechanical and thermal behaviors, paving the way for advancements in flexible semiconductor device manufacturing.

Multiple scattering of local nonlinear resonators on a thin plate

Developing nonlinear resonant metamaterial plates requires an effective wave simulation method to analyze the wave interactions between multiple nonlinear scatterers. The multiple scattering method has the advantages of high computational efficiency and closed-form displacement solutions in modeling the finitely periodic scatterer array. However, the multiple scattering method of nonlinear scatterers is absent in the available studies of plate structures. In this paper, a nonlinear multiple scattering approach is formulated and analyzed for thin plates with nonlinear resonant scatterers. The resonant scatterer consists of an inner plate with a local resonator modeled by the nonlinear mass-spring system. The motion equation of resonant scatterers is solved using the perturbation technology and wave expansion method. The T-matrix of the resonant scatterer of each order is then derived at the arbitrary harmonic frequency from continuous interfacial conditions of resonant scatterers. Finally, the multiple scattering is formulated for a finitely periodic array of resonant scatterers on a thin plate. Numerical simulations capture the scattering characteristics and stop-band formations of fundamental and second-harmonic waves. Furthermore, the finitely periodic array of nonlinear and linear resonant scatterers achieves the nonreciprocal transmission of flexural waves. These numerical results demonstrate the potential applications of the proposed method in designing metamaterial-based plates with complex transmission properties.

A network model to predict ionic transport in porous materials

Understanding the dynamics of electric-double-layer (EDL) charging in porous media is essential for advancements in next-generation energy storage devices. Due to the high computational demands of direct numerical simulations and a lack of interfacial boundary conditions for reduced-order models, the current understanding of EDL charging is limited to simple geometries. Here, we present a network model to predict EDL charging in arbitrary networks of long pores in the Debye-Hückel limit without restrictions on EDL thickness and pore radii. We demonstrate that electrolyte transport is described by Kirchhoff's laws in terms of the electrochemical potential of charge (the valence-weighted average of the ion electrochemical potentials) instead of the electric potential. By employing the equivalent circuit representation suggested by these modified Kirchhoff's laws, our methodology accurately captures the spatial and temporal dependencies of charge density and electric potential, matching results obtained from computationally intensive direct numerical simulations. Our network model provides results up to six orders of magnitude faster, enabling the efficient simulation of a triangular lattice of five thousand pores in 6 min. We employ the framework to study the impact of pore connectivity and polydispersity on electrode charging dynamics for pore networks and discuss how these factors affect the time scale, energy density, and power density of capacitive charging. The scalability and versatility of our methodology make it a rational tool for designing 3D-printed electrodes and for interpreting geometric effects on electrode impedance spectroscopy measurements.

Local Spectral Multiplicity of Selfadjoint Couplings with General Interface Conditions

We consider selfadjoint operators obtained by pasting a finite number of boundary relations with one-dimensional boundary space. A typical example of such an operator is the Schrödinger operator on a star-graph with a finite number of finite or infinite edges and an interface condition at the common vertex. A wide class of “selfadjoint” interface conditions, subject to an assumption which is generically satisfied, is considered. We determine the spectral multiplicity function on the singular spectrum (continuous as well as point) in terms of the spectral data of decoupled operators.

Mathematical Modeling and Numerical Approximation of Heat Conduction in Three-Phase-Lag Solid

In this article, we propose a mathematical model for one-dimensional heat conduction in a three-layered solid considering that an interfacial condition is present for the temperature and heat flux conditions between the layers. The numerical approach is developed by constructing a finite difference scheme to solve the initial boundary–interface problem. The numerical scheme is designed by considering the accuracy of the model on the inner part of each layer, then extending to the interfaces and boundaries by incorporating the continuous interfacial conditions. The finite difference scheme is unconditionally stable, convergent, and easy to implement since it consists of the solution of two algebraic systems. We provide three numerical examples to confirm that our numerical approximation is consistent with the analytical solution and the physical phenomenon.

Study of the shear bond behavior of a hybrid fiber-reinforced concrete (HyFRC) composite slab

In this study, a new composite slab consisting of a prefabricated hybrid fiber-reinforced concrete (HyFRC) formwork and a cast-in-place ordinary concrete (OC) topping was proposed. The HyFRC was comprised of steel and PVA fibers. The compressive strength and bending strength of the HyFRC were 111.4 MPa and 15.6 MPa, respectively, and the toughness coefficient was 12.1 MPa. Seven full-scale composite slabs including six simply-supported slabs and one two-span continuous slab were tested under four-point bending to study the shear bond behavior. Three different contact surface conditions were considered for the simply supported slabs. Despite the different interface conditions, results showed that the interfacial bond strength between the HyFRC formwork and the OC topping was sufficient to achieve flexural failure. Numerical simulation was used to analyze the interfacial force transfer mechanism and was validated by the experimental data. The validated numerical model was used to study the influence of concrete topping depth and amount of reinforcement on the load-carrying capacity and longitudinal shear stress at the joint surface. The numerical results identified a critical condition of positive effect between reinforcement ratio and OC topping thickness. The peak load of a properly designed composite slab was almost two times that of the corresponding slab with ordinary concrete only, and the deformation capability was also larger. A procedure for calculating the flexural capacity of the composite slab was proposed.

Developing organs-on-chips for biomedical applications.

In recent years, organs-on-chips have been arousing great interest for their bionic and stable construction of crucial human organs in vitro. Compared with traditional animal models and two-dimensional cell models, organs-on-chips could not only overcome the limitations of species difference and poor predict ability but also be capable of reappearing the complex cell-cell interaction, tissue interface, biofluid and other physiological conditions of humans. Therefore, organs-on-chips have been regarded as promising and powerful tools in diverse fields such as biology, chemistry, medicine and so on. In this perspective, we present a review of organs-on-chips for biomedical applications. After introducing the key elements and manufacturing craft of organs-on-chips, we intend to review their cut-edging applications in biomedical fields, incorporating biological analysis, drug development, robotics and so on. Finally, the emphasis is focused on the perspectives of organs-on-chips.

Identification of excessive contact pressures under hand orthosis based on finite element analysis.

Implicit magnitudes and distribution of excessive contact pressures under hand orthoses hinder clinicians from precisely adjusting them to relieve the pressures. To address this, contact pressure under a hand orthosis were analysed using finite element method. This paper proposed a method to numerically predict the relatively high magnitudes and critical distribution of contact pressures under hand orthosis through finite element analysis, to identify excessive contact pressure locations. The finite element model was established consisting of the hand, orthosis and bones. The hand and bones were assumed to be homogeneous and elastic bodies, and the orthosis was considered as an isotropic and elastic shell. Two predictions were conducted by assigning either low (fat) or high (skin) material stiffness to the hand model to attain the range of pressure magnitudes. An experiment was conducted to measure contact pressures at the predicted pressure locations. Identical pressure distributions were obtained from both predictions with relatively high pressure values disseminated at 12 anatomical locations. The highest magnitude was found at the thumb metacarpophalangeal joint with the maximum pressure range from 13 to 78 KPa. The measured values were within the predicted range of pressure magnitudes. Moreover, 6 excessive contact pressure locations were identified. The proposed method was verified by the measurement results. It renders understanding of interface conditions underneath the orthosis to inform clinicians regarding orthosis design and adjustment. It could also guide the development of 3D printed or sensorised orthosis by indicating optimal locations for perforations or pressure sensors.

Threshold Voltage Modulation and Gate Leakage Suppression of Enhancement‐Mode GaN HEMT by Metal/Insulator/p‐GaN Gate Structure

Herein, a metal/insulator/p‐GaN gate HEMT (MIP‐HEMT) with Si3N4 gate dielectric layer is fabricated. Compared to the conventional p‐GaN HEMT, the MIP‐HEMT has a higher threshold voltage (Vth) of 4.8 V and better gate leakage suppression. The mechanism of threshold voltage increase in MIP‐HEMT is elucidated through an analysis of the electric field distribution in the gate region and the proposed gate capacitance model. Furthermore, MIP‐HEMTs with gate dielectric layers of varying dielectric constants and thicknesses are designed and simulated. The obtained results lead to the derivation of an empirical formula for the threshold voltage of the MIP‐HEMT under ideal dielectric/p‐GaN interface conditions, in relation to the dielectric constant and thickness of the gate dielectric layer. Additionally, simulations are conducted on the MIP‐HEMT incorporating fixed charges at the dielectric/p‐GaN interface, and the impact of interface charges is investigated. This refinement enables a more accurate prediction of the MIP‐HEMT's threshold voltage. Conclusively, MIP‐HEMTs can effectively modulate the threshold voltage by manipulating the parameters of the gate dielectric layer, thereby extending the threshold voltage range of conventional enhancement‐mode devices.

Streaming Potential and Associated Electrokinetic Effects through a Channel Filled with Electrolyte Solution Surrounded by a Layer of Immiscible and Dielectric Liquid.

The present article deals with the streaming potential-mediated pressure-driven flow across a channel in which the electrolyte solution is surrounded by a layer of cell membrane. Such a membrane of a biological cell may be modeled as an immiscible and dielectric liquid, which may bear free lipid molecules or charged surfactants. The presence of such additional charged molecules may lead to formation of liquid-liquid interfacial charge. In addition, the dielectric gradient-mediated ion partitioning effect further plays an important role in two-phase electrokinetic motion. We aim to study the generation of streaming potential and electrokinetic conversion efficiency as well as associated electroviscous effect for the undertaken problem. The mathematical model is based on the Poisson-Boltzmann equation for electrostatic potential and the Stokes equation for fluid flow, and the problem is studied considering suitable interfacial conditions for the flow variables along the liquid-liquid interface. The explicit analytical results for velocity and streaming field, electrokinetic energy conversion efficiency, and the parameter indicating the electroviscous effect are derived under the Donnan limit and within the Debye-Hückel electrostatic framework. We further numerically calculated the aforementioned intrinsic electrokinetic parameter associated with the problem undertaken for a wide range of pertinent parameters. The results are illustrated to indicate the impact of pertinent parameters on the generation of the streaming potential and associated electrokinetic effects.

Mesoscopic simulations of a fracture process in reinforced concrete beam in bending using a 2D coupled DEM/micro-CT approach

In this study, a complex fracture process in a short rectangular concrete (RC) beam reinforced by one longitudinal bar (without vertical reinforcement) and subjected to quasi-static three-point bending was numerically explored in 2D conditions. A critical diagonal shear crack in the beam caused it to fail during the experiment. The numerical simulations were conducted with a classical particle discrete element method (DEM). A three-phase concrete description (aggregate, mortar, and interfacial transitional zones (ITZs) around aggregates) accounted for the concrete heterogeneity. In mesoscopic DEM calculations based on a 2D X-ray CT scan, the actual shape and placement of aggregate particles in concrete were taken for granted. In the study, the steel bar with ribs was replicated. ITZ was also assumed between the bar and mortar. Without imposing any bond-slip law, a geometrical bar/concrete interface condition was explicitly considered. The focus was on the force–deflection diagram, fracture process, contact forces, and stresses along the bar. A good level of agreement about the evolution of the vertical force versus the deflection and failure mechanism was attained between DEM analyses and in-house laboratory tests despite simplified 2D conditions. A strong effect of concrete mesostructure on the crack pattern was found.

An Inverse Problem for Estimating Spatially and Temporally Dependent Surface Heat Flux with Thermography Techniques

In this study, an inverse conjugate heat transfer problem is examined to estimate temporally and spatially the dependent unknown surface heat flux using thermography techniques with a thermal camera in a three-dimensional domain. Thermography techniques encompass a broad set of methods and procedures used for capturing and analyzing thermal data, while thermal cameras are specific tools used within those techniques to capture thermal images. In the present study, the interface conditions of the plate and air domains are obtained using perfect thermal contact conditions, and therefore we define the problem studied as an inverse conjugate heat transfer problem. Achieving the simultaneous solution of the continuity, Navier–Stokes, and energy equations within the air domain, alongside the heat conduction equation in the plate domain, presents a more intricate challenge compared to conventional inverse heat conduction problems. The validity of our inverse solutions was verified through numerical simulations, considering various inlet air velocities and plate thicknesses. Notably, it was found that due to the singularity of the gradient of the cost function at the final time point, the estimated results near the final time must be discarded, and exact measurements consistently produce accurate boundary heat fluxes under thin-plate conditions, with air velocity exhibiting no significant impact on the estimates. Additionally, an analysis of measurement errors and their influence on the inverse solutions was conducted. The numerical results conclusively demonstrated that the maximum error when estimating heat flux consistently remained below 3% and higher measurement noise resulted in the accuracy of the heat flux estimation decreasing. This underscores the inherent challenges associated with inverse problems and highlights the importance of obtaining accurate measurement data in the problem domain.