Interface Assembly Research Articles

PENGEMBANGAN SIMULASI VIRTUAL MESIN SIZING SEBAGAI ALTERNATIF METODE PEMBELAJARAN PRAKTIKUM TEKNOLOGI PERSIAPAN PERTENUNAN

This study has developed a virtual simulation of a sizing machine as an alternative approach for practical learning in weaving preparation technology. This simulation was created by emulating the interface and functionalities of an industrial sizing machine. This study applies the five-stage Research and Development (R&D) ADDIE model developed by Robert Maribe Branch in 2009. The first stage is analysis, then design, development, implementation, and evaluation. The design and development method of this simulation employs graphic design applications CorelDraw, Adobe Illustrator, and Animate CC for component production, user interface assembly, encoding, movie testing, and publication. A Likert scale questionnaire with a 1–5 scale is used to evaluate the user experience and determine whether the simulation content is adequate. Using the validity and practicality criteria, the percentage value is then calculated from the entire score. According to the study's findings, the simulation sizing machine's concept and operation were given a 100% validity percentage with a very valid category, and the degree of fit between the simulation's features and the industry-standard sizing machine was given a 90% validity percentage with a very valid category. Additionally, with a percentage of 79.45%, the user evaluation results of the virtual simulation of the sizing machine indicated that this program was feasible to use. It is possible to employ the produced virtual simulation of the size machine as an alternate approach to hands-on learning in weaving preparation.

Biphasic interface templated synthesis of wrinkled MOFs for the construction of cascade sensing platform based on the encapsulated gold nanoclusters and enzymes

The design and construction of MOFs with flower-like structure could afford sufficient space for the immobilization of guests with large size and interconnected transport channels for their mass diffusion although it remains a challenge. Herein, wrinkled Ce-based hierarchically porous UiO-66 (Ce-WUiO-66) with good crystallinity was successfully synthesized for the first time using bicontinuous emulsion composed of 1-heptanol, water and F127 (PEO106PPO70PEO106) surfactant as a template. F127 played a key role in the formation of emulsions as a stabilizer, and meanwhile its PEO segments interacted with MOF precursors to guide the evolvement of crystallized pore walls. Through controlling the ratios of heptanol to water and the salinity, the distances of the pleat openings and the morphology of the resultant Ce-WUiO-66 were facilely regulated. In virtue of its highly open radial structure, Ce-WUiO-66 could serve as an ideal platform for loading multiple substances to build a cascade sensing system. As a proof of concept, we designed an amino acid (AA) cascade probe by co-immobilizing gold nanoclusters (AuNCs) and LAA oxidase into Ce-WUiO-66. The aggregation-induced-emission enhancement resulted from the encapsulation of AuNCs into Ce-WUiO-66 significantly improved the detection sensitivity and the detection limit of corresponding substrates reached as low as 10-8 M. The proposed biphasic interface assembly strategy is hopefully to provide a new route for the rational design of MOFs with various open pore structure and broaden their potential applications with multiple large-size substances involved besides the currently exemplified cascade sensing platform.

Drug-Loaded Mesoporous Polydopamine Nanoparticles in Chitosan Hydrogels Enable Myocardial Infarction Repair through ROS Scavenging and Inhibition of Apoptosis.

In this study, we synthesized mesoporous polydopamine nanoparticles (MPDA NPs) using an emulsion-induced interface assembly strategy and loaded epigallocatechin gallate (EGCG) into MPDA NPs via electrostatic attraction to form EGCG@MPDA NPs. In the post myocardial infarction (MI) environment, these interventions specifically aimed to eliminate reactive oxygen species (ROS) and facilitate the repair of MI. We further combined them with a thermosensitive chitosan (CS) hydrogel to construct an injectable composite hydrogel (EGCG@MPDA/CS hydrogel). Utilizing in vitro experiments, the EGCG@MPDA/CS hydrogel exhibited excellent ROS-scavenging ability of H9C2 cells under the oxidative stress environment and also could inhibit their apoptosis. The EGCG@MPDA/CS hydrogel significantly promoted left ventricular ejection fraction (LVEF) in infarcted rat models post injection for 28 days compared to the PBS group (51.25 ± 1.73% vs 29.31 ± 0.78%, P < 0.05). In comparison to the PBS group, histological analysis revealed a substantial increase in left ventricular (LV) wall thickness in the EGCG@MPDA/CS hydrogel group (from 0.58 ± 0.03 to 1.39 ± 1.11 mm, P < 0.05). This work presents a novel approach to enhance MI repair by employing the EGCG@MPDA/CS hydrogel. This hydrogel effectively reduces local oxidative stress by ROS and stimulates the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway.

Construction of oxime functionalized mesoporous polydopamine spheres based on emulsion-induced interface assembly approach for efficient extraction of uranium

Construction of oxime functionalized mesoporous polydopamine spheres based on emulsion-induced interface assembly approach for efficient extraction of uranium

Electrobiofabrication of antibody sensor interfaces within a 3D printed device yield rapid and robust electrochemical measurements of titer and glycan structure.

We report the integration of 3D printing, electrobiofabrication, and protein engineering to create a device that enables near real-time analysis of monoclonal antibody (mAb) titer and quality. 3D printing was used to create the macroscale architecture that can control fluidic contact of a sample with multiple electrodes for replicate measurements. An analysis "chip" was configured as a "snap-in" module for connecting to a 3D printed housing containing fluidic and electronic communication systems. Electrobiofabrication was used to functionalize each electrode by the assembly of a hydrogel interface containing biomolecular recognition and capture proteins. Specifically, an electrochemical thiol oxidation is used to assemble a thiolated polyethylene glycol hydrogel, that in turn is covalently coupled to either a cysteine-tagged protein G that binds the antibody's Fc region or a lectin that binds the glycans of target mAb analytes. We first show the design, assembly, and testing of the hardware device. Then, we show the transition of a step-by-step sensing methodology (e.g., mix, incubate, wash, mix, incubate, wash, measure) into the current method where functionalization, antibody capture, and assessment are performed in situ and in parallel channels. Both titer and glycan analyses were found to be linear with antibody concentration (to 0.2 mg/L). We further found the interfaces could be reused with remarkably similar results. Because the interface assembly and use are simple, rapid, and robust, we suggest this assessment methodology will be widely applicable, including for other biomolecular process development and manufacturing environments.

Designer Metasurfaces via Nanocube Assembly at the Air-Water Interface.

The advent of metasurfaces has revolutionized the design of optical instruments, and recent advancements in fabrication techniques are further accelerating their practical applications. However, conventional top-down fabrication of intricate nanostructures proves to be expensive and time-consuming, posing challenges for large-scale production. Here, we propose a cost-effective bottom-up approach to create nanostructure arrays with arbitrarily complex meta-atoms displaying single nanoparticle lateral resolution over submillimeter areas, minimizing the need for advanced and high-cost nanofabrication equipment. By utilizing air/water interface assembly, we transfer nanoparticles onto templated polydimethylsiloxane (PDMS) irrespective of nanopattern density, shape, or size. We demonstrate the robust assembly of nanocubes into meta-atoms with diverse configurations generally unachievable by conventional methods, including U, L, cross, S, T, gammadion, split-ring resonators, and Pancharatnam-Berry metasurfaces with designer optical functionalities. We also show nanocube epitaxy at near ambient temperature to transform the meta-atoms into complex continuous nanostructures that can be swiftly transferred from PDMS to various substrates via contact printing. Our approach potentially offers a large-scale manufacturing alternative to top-down fabrication for metal nanostructuring, unlocking possibilities in the realm of nanophotonics.

Liquid Interfacial Gating of Superhydrophobic Plasmonic Metal-Organic Frameworks for Three-in-One Separation, Enrichment, and Recognition in Bacterial Quorum Sensing.

Poor adsorption properties of nonadsorbing targets and competing adsorption of nontargets at a liquid interface always hamper the development of interface sensing techniques. There is a need to fabricate materials that are applicable to various interface assemblies and, meanwhile, could be employed as interfacial gating to improve the performance of interface sensing by separating, enriching, and recognizing targets at the liquid interface. Here, superhydrophobic zeolite imidazole frameworks-8@gold nanoparticles-1H,1H,2H,2H-perfluorodecanethiol (ZIF-8@GNPs-PFDT) with a static water contact angle (WCA) of 155° was constructed via electrostatic self-assembly and surface graft modification. The plasmonic metal-organic framework (PMOF) nanohybrid realized all-purpose self-assembly at air/liquid and liquid/liquid interfaces and also facilely assembled on the surface of liquid droplets, hydrogels, and foams. The self-assembled porous materials displayed the capability for separating, enriching, and recognizing analytes at various oil/water interfaces and thus could be used to adsorb nonadsorbing targets and block the competing adsorption of nontargets. The self-assembled ZIF-8@GNPs-PFDT structures were employed as a three-in-one interfacial gating to endow the excellent surface-enhanced Raman scattering (SERS) sensing capability and has become a promising tool for dye molecular analysis, oil/water separation, organic phase identification, and in situ cultivation and monitoring of bacterial quorum sensing (QS).

Cosmetically Approved Short-Chain Alcohol/Triethyl Citrate/Water Surfactant-Free Microemulsions and Potential Application to Transdermal Penetration of α-Arbutin.

Surfactant-free microemulsions (SFMEs) exhibited remarkable advantages and potential, attributed to their similarity to traditional surfactant-based microemulsions and the absence of surfactants. Herein, a novel SFME was developed utilizing cosmetically approved materials, such as short-chain alcohol as an amphi-solvent, triethyl citrate (TEC) as the nonpolar phase, and water as the polar phase. 1,2-Pentanediol (PtDO)/TEC/water combination can form the largest monophasic zone, accounting for ∼74% of the total phase diagram area, due to an optimal hydrophilic (water)-lipophilic (TEC) balance. Comparable to surfactant-based microemulsion, PtDO/TEC/water SFME can also be categorized into three types: water-in-oil, discontinuous, and oil-in-water. As TEC or water is increased, or PtDO is decreased, the nanoaggregates in PtDO/TEC/water SFME grow from <5 nm to tens of nanometers. The addition of α-arbutin (ABN) does not disrupt PtDO/TEC/water SFME, but rather enhances its formation, resulting in a larger monophasic area and consistent size (2.8-3.8 nm) through participating in interface assembly. Furthermore, ABN-loaded PtDO/TEC/water SFME exhibits remarkable resistance to dilution, exceptional stability, and minimal irritation. Notably, PtDO/TEC/water SFME significantly boosts ABN's solubility in water by 2 times, its percutaneous penetration rate by 3-4 times, and enables a slow-release DPPH• radical scavenging effect. This SFME serves as a safe and cosmetically suitable nanoplatform for the delivery of bioactive substances.

Development of Au Nanoparticle Two-Dimensional Assemblies Dispersed with Au Nanoparticle-Nanostar Complexes and Surface-Enhanced Raman Scattering Activity.

We recently found that polyvinylpyrrolidone (PVP)-protected metal nanoparticles dispersed in water/butanol mixture spontaneously float to the air/water interface and form two-dimensional assemblies due to classical surface excess theory and Rayleigh-Bénard-Marangoni convection induced by butanol evaporation. In this study, we found that by leveraging this principle, a unique structure is formed where hetero gold nanospheres (AuNPs)/gold nanostars (AuNSs) complexes are dispersed within AuNP two-dimensional assemblies, obtained from a mixture of polyvinylpyrrolidone-protected AuNPs and AuNSs that interact electrostatically with the AuNPs. These structures were believed to form as a result of AuNPs/AuNSs complexes formed in the water/butanol mixture floating to the air/water interface and being incorporated into the growth of AuNP two-dimensional assemblies. These structures were obtained by optimizing the amount of mixed AuNSs, with excessive addition resulting in the formation of random three-dimensional network structures. The AuNP assemblies dispersed with AuNPs/AuNSs complexes exhibited significantly higher Raman (surface-enhanced resonance Raman scattering: SERRS) activity compared to simple AuNP assemblies, while the three-dimensional network structure did not show significant SERRS activity enhancement. These results demonstrate the excellent SERRS activity of AuNP two-dimensional assemblies dispersed with hetero AuNPs/AuNSs complexes.

Diverging co-translational protein complex assembly pathways are governed by interface energy distribution

Protein-protein interactions are at the heart of all cellular processes, with the ribosome emerging as a platform, orchestrating the nascent-chain interplay dynamics. Here, to study the characteristics governing co-translational protein folding and complex assembly, we combine selective ribosome profiling, imaging, and N-terminomics with all-atoms molecular dynamics. Focusing on conserved N-terminal acetyltransferases (NATs), we uncover diverging co-translational assembly pathways, where highly homologous subunits serve opposite functions. We find that only a few residues serve as “hotspots,” initiating co-translational assembly interactions upon exposure at the ribosome exit tunnel. These hotspots are characterized by high binding energy, anchoring the entire interface assembly. Alpha-helices harboring hotspots are highly thermolabile, folding and unfolding during simulations, depending on their partner subunit to avoid misfolding. In vivo hotspot mutations disrupted co-translational complexation, leading to aggregation. Accordingly, conservation analysis reveals that missense NATs variants, causing neurodevelopmental and neurodegenerative diseases, disrupt putative hotspot clusters. Expanding our study to include phosphofructokinase, anthranilate synthase, and nucleoporin subcomplex, we employ AlphaFold-Multimer to model the complexes’ complete structures. Computing MD-derived interface energy profiles, we find similar trends. Here, we propose a model based on the distribution of interface energy as a strong predictor of co-translational assembly.

Rationally Designed Ionically Conductive Metal‐Organic Frameworks for Ultrasensitive Methamphetamine Analogs Sensor

AbstractAchieving convenient, sensitive, low‐cost, and non‐contact detection of trace addictive drugs is a challenging problem. Herein, a novel ionically conductive metal‐organic framework (IC‐MOFs) is designed through a controlled interface assembly strategy. The active metal anions are incorporated into the layered MOFs with a porous structure, forming charge carriers that served as effective adsorption/binding sites for N‐methylphenethylamine (MPEA), a crucial simulator of addictive drugs. The in situ integrated Zn3(HHTP)2‐MOF sensor device designed in this study demonstrated real‐time detection of sub‐ppb level MPEA at room temperature, with an exceptionally low theoretical detection limit of 20 ppt. The overall sensing performance of this sensor surpassed all previously reported chemical sensors for detecting methamphetamine. The Zn3(HHTP)2‐MOF sensor exhibited outstanding selectivity, rapid response time (ca. 5 s), excellent long‐term stability, amenable miniaturization, and device consistency. The device successfully passed dual 85 test (500 h at 85 °C and 85% of humidity), which is rarely report previously. Density functional theoretical calculations (DFT) and spectral characterization confirmed that the prominent selectivity of Zn3(HHTP)2‐MOF toward MPEA is attributed to the strong binding ability. The general and straightforward strategy provides brand‐new route to exploiting smart sensors for drug prevention and surveillance.

Effect of Carboxyl Group Position on Assembly Behavior and Structure of Hydrocarbon Oil-Carboxylic Acid Compound Collector on Low-Rank Coal Surface: Sum-Frequency Vibration Spectroscopy and Coarse-Grained Molecular Dynamics Simulation Study.

In this work, the assembly behavior and structure of a compound collector with different carboxyl group positions at the low-rank coal (LRC)-water interface were investigated through coarse-grained molecular dynamics simulation (CGMD) combined with sum-frequency vibration spectroscopy (SFG). The choice of compound collector was dodecane +decanoic acid (D-DA) and dodecane +2-butyl octanoic acid (D-BA). CGMD results showed that the carboxyl group at the carbon chain's middle can better control the assembly process between carboxylic acid and D molecules. SFG research found that the carboxyl group at the carbon chain's termination had a greater impact on the displacement of the methyl/methylene symmetric stretching vibration peak, while the carboxyl group at the carbon chain's middle had a greater impact on the displacement of the methyl/methylene asymmetric stretching vibration peak. The spatial angle calculation results revealed that the methyl group's orientation angle in the D-BA molecule was smaller and the carboxyl group's orientation angle in the BA molecule was bigger, indicating that D-BA spread more flatly on the LRC surface than D-DA. This meant that the assembled structure had a larger effective adsorption area on the LRC surface. The flotation studies also verified that the assembly behavior and structure of D-BA with the carboxyl group at the carbon chain's middle at the LRC-water interface were more conducive to the improvement of flotation efficiency. The study of interface assembly behavior and structure by CGMD combined with SFG is crucial for the creation of effective compound collectors.

Graphite–MoS2 composite thin films with photoconductivity synthesized using liquid interface assembly

Graphite–MoS2 composite thin films with photoconductivity synthesized using liquid interface assembly

Ultrathin coordination-crosslinked nacre-inspired hydrogel superwetting membranes with enhanced mechanical stability for high-performance emulsion separation

Ultrathin coordination-crosslinked nacre-inspired hydrogel superwetting membranes with enhanced mechanical stability for high-performance emulsion separation

Preparation and sensitive SERS properties of sapphire-supported plasmonic Ag/Au hybrid coupled nanoarray

Preparation and sensitive SERS properties of sapphire-supported plasmonic Ag/Au hybrid coupled nanoarray

Electrochemical and optical studies on photoactive BiVO4-TiO2/poly 3,4-ethylenedioxythiophene assemblies in gel electrolyte: Role of inorganic/organic interfaces in surface functionalization

Inorganic/organic interface assemblies were created from poly 3,4-ethylenedioxythio­phene (PEDOT) interfaced with amorphous BiVO4 and with BiVO4-TiO2. Electrochemical cells-based thermoplastic gel electrolytes containing KI/I2 were used to study the photoelectrochemical behavior of the Inorganic/organic interface electrodes. Optical studies show that doping BiVO4 with TiO2 narrowed the optical band gap to allow more absorption in the visible region and increases solar energy conversion. Evidence for both direct and indirect band gaps was observed. Refractive index data indicates that BiVO4 and BiVO4/TiO2 obey the anomalous dispassion multiple-oscillator model. Chronoampero­metry of these assemblies shows the phenomena of dark current, which correlates to the presence of random electron/hole generation in the depletion layer. PEDOT enhances the photoactivity of BiVO4 only. Electrochemical impedance spectroscopy studies indicated that both kinetic and diffusional control at high and low frequencies, respectively. Furthermore, studies show that as frequency increases, the conductivity increases due to dispersion and charge carrier hopping. All photoactivity outcomes were reproducible.

Effect of tannic acid modification on the interface and emulsification properties of zein colloidal particles.

Interface modification driven by supramolecular self-assembly has been accepted as a valuable strategy for emulsion stabilization enhancement. However, there has been a dearth of comparative research on the effect of simple complexation and assembly from the perspective of mechanism. This work selected zein and tannic acid (TA) as representative protein and polyphenol modules for self-assembly (coined as TA-modified zein particle and TA-zein complex particle) to explore the surface properties, interfacial behavior, as well as the stability of constructed Pickering emulsions to obtain the regulation law of different modification methods on the interfacial behavior of colloidal particles. The results demonstrated that TA-modified zein colloidal particles stood a chance of improving emulsifying properties. When the TA concentration was 3 mmol/L, the optimized TA-modified zein particle was nano-sized (109.83 nm) and had advantageous interfacial properties, including sharply reduced surface hydrophobicity, and low diffusion rate at the oil/water interface. As a result, the shelf life of Pickering emulsion containing 50% oil phase was extended to 90 days. Through multi-angled research on the properties of the interfacial membrane, improvement of emulsion stability was due to the formation of viscoelastic interfacial film which resulted from the decrease of absorption rate between particles and interface. Using refined regulation to study the role of different sample preparation methods from a mechanistic perspective. Overall, this study has provided a reference for TA to regulate the surface properties and interface behavior of zein colloidal particles, enriched the understanding of colloidal interface assembly, and provided a theoretical basis for the quality control of interface-oriented food systems. This article is protected by copyright. All rights reserved.

Interface Assembly of Polymer Networks on Metal–Organic Frameworks for the Engineering of Functional Nanoparticles

Interface Assembly of Polymer Networks on Metal–Organic Frameworks for the Engineering of Functional Nanoparticles

Spiropyran-Based Soft Substrate with SPR, Anti-Reflection and Anti-NRET for Enhanced Visualization/Fluorescence Dual Response to Metal Ions.

The photoluminescence of modified spiropyran on solid surfaces is poor, and the fluorescence intensity of its MC form is weak, which affects its application in the field of sensing. In this work, a PMMA layer containing Au nanoparticles and a spiropyran monomolecular layer are coated on the surface of a PDMS substrate with inverted micro-pyramids successively by means of interface assembly and soft lithography, and the overall structure is similar to insect compound eyes. The anti-reflection effect of the bioinspired structure, the SPR (surface plasmon resonance) effect of the Au nanoparticles and the anti-NRET (non-radiation energy transfer) effect of the PMMA isolation layer raise the fluorescence enhancement factor of the composite substrate vs. the surface MC form of spiropyran to 5.06. In the process of metal ion detection, the composite substrate can achieve both colorimetric and fluorescence response, and the detection limit for Zn2+ can reach 0.281 μM. However, at the same time, the lack of the ability to recognize specific metal ions is expected to be further improved by the modification of spiropyran.

Mechanism of Reduced Glutathione Induced Lysozyme Defolding and Molecular Self-Assembly.

The distinctive assembly behaviors of lysozyme (Lys) feature prominently in food, materials, biomedicine, and other fields and have intrigued many scholars. Although our previous work suggested that reduced glutathione (GSH) could induce lysozyme to form interfacial films at the air/water interface, the underlying mechanism is still obscure. In the present study, the effects of GSH on the disulfide bond and protein conformation of lysozyme were investigated by fluorescence spectroscopy, circular dichroism spectroscopy, and infrared spectroscopy. The findings demonstrated that GSH was able to break the disulfide bond in lysozyme molecules through the sulfhydryl/disulfide bond exchange reaction, thereby unraveling the lysozyme. The β-sheet structure of lysozyme expanded significantly, while the contents of α-helix and β-turn decreased. Furthermore, the interfacial tension and morphology analysis supported that the unfolded lysozyme tended to arrange macroscopic interfacial films at the air/water interface. It was found that pH and GSH concentrations had an impact on the aforementioned processes, with higher pH or GSH levels having a positive effect. This paper on the exploration of the mechanism of GSH-induced lysozyme interface assembly and the development of lysozyme-based green coatings has better instructive significance.