Interesting Profile Research Articles

Volatilomics for halal and non-halal meatball authentication using solid-phase microextraction–gas chromatography–mass spectrometry

The adulteration of beef meatballs with wild boar (Sus scrova) meat or chicken may be undertaken for economic reasons. This adulteration is a very sensitive issue, particularly for Muslim consumers, as the consumption of wild boar is strictly prohibited by Islamic law. This study aimed to discriminate volatile compounds in meatballs made from beef, chicken, and wild boar and mixtures thereof using solid-phase microextraction–gas chromatography–mass spectrometry (SPME/GC–MS) and multivariate data analysis. SPME is a non-destructive method for the extraction of volatile compounds and does not alter the original chemical composition of the volatiles. A validated partial least squares discriminant analysis (PLS-DA) model with three classes was used to uncover the discriminating volatiles of each type of meatball. The results indicated that β-cymene, 3-methyl-butanal, and 2-pentanol were among the positive discriminating volatiles with the highest variable importance in projection (VIP) values among the chicken meatballs. The highest VIP positive discriminating volatiles in the beef meatballs were 5-ethyl-m-xylene, benzaldehyde, and 3-ethyl-2-methyl-1,3-hexadiene. The mixed meatballs exhibited an interesting profile, with all appearing in the same group as the pure wild boar meatballs. However, the discriminating volatiles derived from a separate PLS-DA model indicated that they contained different compounds. In the pure wild boar meatballs, six compounds (pentanal, 2,6-dimethylcyclohexanone, 1-undecanol, cyclobutanol, 2,4,5-trimethyl-thiazole, and 5-ethyl-3-(3-methyl-5-phenyl pyrazol-1-yl)-1,2,4-triazol-4-amine) were identified as discriminating volatile compounds with the highest VIP values. These compounds were consistently found as significant discriminating volatile compounds in mixture meatballs group although with different VIP value. This research demonstrated that SPME-GC/MS combined with multivariate data analysis was a fast and reliable method for differentiating meatballs made from beef, chicken, and wild boar meat based on their volatile compound contents.

Stability and change in vocational interests after graduation from high school: A six-wave longitudinal study.

Vocational interests shape major life decisions and predict major life outcomes. Therefore, it is important to understand how vocational interests develop in young adulthood, a time when young people begin to make their own life decisions. In the present study, we investigated stability and change in vocational interests across a time span of 10 years, including the transition from high school to postsecondary education and the transition into the labor market. Using a large data set comprised of 3,023 German young adults, we provide descriptive information about the longitudinal development of vocational interests across 6 equally spaced time points. We investigated 5 different indicators of stability and change: rank-order stabilities, mean-level changes, changes in variance, profile stabilities, and profile differentiation, as well as gender differences in these indicators. We found high stabilities for the interest scales and interest profiles that increased even more across the period of 10 years. Substantial changes in mean levels occurred primarily in the context of the transition from high school to university, to vocational training, or into the labor market. As expected, there were gender differences in the mean levels, but the developmental patterns in the trajectories of vocational interests were very similar for men and women. Overall, our findings indicate that longitudinal studies with multiple time points are needed to extend knowledge about interest development. In addition, our findings demonstrate that considering normative social transitions may be key to better understanding longitudinal interest development. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Biomimetic Approaches to the Synthesis of Natural Disesquiterpenoids: An Update.

Natural disesquiterpenoids represent a small group of secondary metabolites characterized by complex molecular scaffolds and interesting pharmacological profiles. In the last decade, more than 400 new disesquiterpenoids have been discovered and fully characterized, pointing out once more the “magic touch” of nature in the design of new compounds. The perfect blend of complex and unique architectures and biological activity has made sesquiterpene dimers an attractive and challenging synthetic target, inspiring organic chemists to find new and biomimetic approaches to replicate the efficiency and the selectivity of natural processes under laboratory conditions. In this work, we present a review covering the literature from 2010 to 2020 reporting all the efforts made in the total synthesis of complex natural disesquiterpenoids.

Synthesis of equilibria in infinite-duration games on graphs

In this survey, we propose a comprehensive introduction to game theory applied to computer-aided synthesis. We study multi-player turn-based infinite-duration games played on a finite directed graph such that each player aims at maximizing a payoff function. We present the well-known notions of Nash equilibrium and subgame perfect equilibrium, as well as interesting strategy profiles of players as response to the strategy announced by a specific player. We provide classical and recent results about the related threshold synthesis problem.

Beneficial Effects of Choline Alphoscerate on Amyloid-β Neurotoxicity in an In vitro Model of Alzheimer's Disease.

Alzheimer's disease (AD) is the most common form of neurodegenerative disorder characterized by cognitive impairment, which represents an urgent public health concern. Given the worldwide impact of AD, there is a compelling need for effective therapies to slow down or halt this disorder. Choline alphoscerate (α-GPC) represents a potentially effective cholinergic neurotransmission enhancing agent with an interesting clinical profile in cognitive dysfunctions improvement, although only scanty data are available about the mechanisms underlying such beneficial effects. The SH-SY5Y neuronal cell line, differentiated for 1 week with 10 μm of all-trans-retinoic acid (RA), to achieve a switch towards a cholinergic phenotype, was used as an in vitro model of AD. SH-SY5Y cells were pre-treated for 1h with α-GPC (100nM) and treated for 72 h with Aβ25-35 (10μM). α-GPC was able to antagonize Aβ25-35 mediated neurotoxicity and attenuate the Aβ-induced phosphorylation of the Tau protein. Moreover, α-GPC exerted its beneficial effects by employing the NGF/TrkA system, knocked down in AD and, consequently, by sustaining the expression level of synaptic vesicle proteins, such as synaptophysin. Taken together, our data suggest that α-GPC can have a role in neuroprotection in the course of toxic challenges with Aβ. Thus, a deeper understanding of the mechanism underlying its beneficial effect, could provide new insights into potential future pharmacological applications of its functional cholinergic enhancement, with the aim to mitigate AD and could represent the basis for innovative therapy.

Rogue wave signals in a coupled anharmonic network: effects of the transverse direction

In this paper, we consider the dynamics of modulated waves in a nonlinear two-dimensional discrete electrical lattice made of several well-known anharmonic modified Noguchi lines coupled transversely to one another with a linear inductor. In the linear limit and depending on the chosen frequency domain, we demonstrate that the network behaves either as a right-handed line or as a left-handed line. We also establish that the band pass character of the modified Noguchi line increases the bandwidth of the allowed frequencies for which the system adopts the left-handed behavior. Based on the reductive perturbation method in the semi-discrete approximation, we derive a (2 + 1)-dimensional nonlinear Schrodinger (2D-NLS) equation governing the dynamics of the small amplitude signals in the system. The specific nature of this amplitude equation is examined, and it appears that its hyperbolic or elliptic character strongly depends on the coupling inductor $$L_{2}$$ . Moreover, using a simple transformation, we reduce the obtained equation to a (1 + 1)-dimensional nonlinear Schrodinger equation (1D-NLS). The exact first- and second-order rogue wave’s solutions of this 2D-NLS equation are derived in bandwidth frequencies where the network may exhibit modulational instability. The results of our investigations display number of interesting rogue wave profiles which have not been observed in the scientific literature. Furthermore, we examine the effects of both the coupling linear inductor $$L_{2}$$ and the nonlinear dispersion factor $$\eta$$ on the characteristics of the rogue waves and also on their propagation through the network. It appears that $$L_{2}$$ and $$\eta$$ can be used to squeeze or stretch the shape of the rogue waves signals traveling in the network. These numerical results are in perfect agreement with the analytical predictions.

Cannabidiol and Other Cannabinoids in Demyelinating Diseases

A growing body of preclinical evidence indicates that certain cannabinoids, including cannabidiol (CBD) and synthetic derivatives, may play a role in the myelinating processes and are promising small molecules to be developed as drug candidates for management of demyelinating diseases such as multiple sclerosis (MS), stroke and traumatic brain injury (TBI), which are three of the most prevalent demyelinating disorders. Thanks to the properties described for CBD and its interesting profile in humans, both the phytocannabinoid and derivatives could be considered as potential candidates for clinical use. In this review we will summarize current advances in the use of CBD and other cannabinoids as future potential treatments. While new research is accelerating the process for the generation of novel drug candidates and identification of druggable targets, the collaboration of key players such as basic researchers, clinicians and pharmaceutical companies is required to bring novel therapies to the patients.

Sustainable Myrcene-Based Elastomers via a Convenient Anionic Polymerization.

Soluble heterocomplexes consisting of sodium hydride in combination with trialkylaluminum derivatives have been used as anionic initiating systems at 100 °C in toluene for convenient homo-, co- and ter-polymerization of myrcene with styrene and isoprene. In this way it has been possible to obtain elastomeric materials in a wide range of compositions with interesting thermal profiles and different polymeric architectures by simply modulating the alimentation feed and the (monomers)/(initiator systems) ratio. Especially, a complete study of the myrcene-styrene copolymers (PMS) was carried out, highlighting their tapered microstructures with high molecular weights (up to 159.8 KDa) and a single glass transition temperature. For PMS copolymer reactivity ratios, rmyr = 0.12 ± 0.003 and rsty = 3.18 ± 0.65 and rmyr = 0.10 ± 0.004 and rsty = 3.32 ± 0.68 were determined according to the Kelen–Tudos (KT) and extended Kelen–Tudos (exKT) methods, respectively. Finally, this study showed an easy accessible approach for the production of various elastomers by anionic copolymerization of renewable terpenes, such as myrcene, with commodities.

Synthesis of Natural Product-Like Tricyclic Higher-Carbon Sugar Nucleosides

Because of the structural novelty and interesting biological profiles, the synthesis of higher-carbon sugars has been highly pursued. In this work, we first synthesized a series of structurally novel bis-uracil containing tricyclic higher-carbon sugar nucleosides (4a–e) using D-xylose as the starting material and the classical Vorbruggen glycosylation as the key synthetic step. The yields of the target compound were good. Unfortunately, despite the presence of pharmaceutically relevant uracil fragment, compounds 4a–e were inactive against the proliferation of several cancer cell lines (EC109, EC9706, PC-3, and MGC-803). Whether and how 4a–e functioned as anticancer agents would be further studied in our laboratory.

New Avenues for Phosphodiesterase Inhibitors in Asthma.

IntroductionPhosphodiesterases (PDEs) are isoenzymes ubiquitously expressed in the lungs where they catalyse cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (GMP), which are fundamental second messengers in asthma, thereby regulating the intracellular concentrations of these cyclic nucleotides, their signaling pathways and, consequently, myriad biological responses. The superfamily of PDEs is composed of 11 families with a distinct substrate specificity, molecular structure and subcellular localization. Experimental studies indicate a possible role in asthma mainly for PDE3, PDE4, PDE5 and PDE7. Consequently, drugs that inhibit PDEs may offer novel therapeutic options for the treatment of this disease.Areas CoveredIn this article, we describe the progress made in recent years regarding the possibility of using PDE inhibitors in the treatment of asthma.Expert OpinionMany data indicate the potential benefits of PDE inhibitors as an add-on treatment especially in severe asthma due to their bronchodilator and/or anti-inflammatory activity, but no compound has yet reached the market as asthma treatment mainly because of their limited tolerability. Therefore, there is a growing interest in developing new PDE inhibitors with an improved safety profile. In particular, the research is focused on the development of drugs capable of interacting simultaneously with different PDEs, or to be administered by inhalation. CHF 6001 and RPL554 are the only molecules that currently are under clinical development but there are several new agents with interesting pharmacological profiles. It will be stimulating to assess the impact of such agents on individual treatable traits in specially designed studies.

Temporal evolution of small-scale internetwork magnetic fields in the solar photosphere

Context. While the longitudinal field that dominates in photospheric network regions has been studied extensively, small-scale transverse fields have recently been found to be ubiquitous in the quiet internetwork photosphere and this merits further study. Furthermore, few observations have been able to capture how this field evolves. Aims. We aim to statistically characterize the magnetic vector in a quiet Sun internetwork region and observe the temporal evolution of specific small-scale magnetic features. Methods. We present two high spatio-temporal resolution observations that reveal the dynamics of two disk-centre internetwork regions taken by the new GREGOR Infrared Spectrograph Integral Field Unit with the highly magnetically sensitive photospheric Fe I line pair at 15648.52 Å and 15652.87 Å. We record the full Stokes vector and apply inversions with the Stokes inversions based on response functions code to retrieve the parameters characterizing the atmosphere. We consider two inversion schemes: scheme 1 (S1), where a magnetic atmosphere is embedded in a field free medium, and scheme 2 (S2), with two magnetic models and a fixed 30% stray light component. Results. The magnetic properties produced from S1 inversions returned a median magnetic field strength of 200 and 240 G for the two datasets, respectively. We consider the median transverse (horizontal) component, among pixels with Stokes Q or U, and the median unsigned longitudinal (vertical) component, among pixels with Stokes V, above a noise threshold. We determined the former to be 263 G and 267 G, and the latter to be 131 G and 145 G, for the two datasets, respectively. Finally, we present three regions of interest, tracking the dynamics of small-scale magnetic features. We apply S1 and S2 inversions to specific profiles of interest and find that the latter produces better approximations when there is evidence of mixed polarities. We find patches of linear polarization with magnetic flux density of the order of 130−150 G and find that linear polarization appears preferentially at granule-intergranular lane boundaries. The weak magnetic field appears to be organized in terms of complex ‘loop-like’ structures, with transverse fields often flanked by opposite polarity longitudinal fields.

GC/MS Profiling, In Vitro and In Silico Pharmacological Screening and Principal Component Analysis of Essential Oils from Three Exotic and Two Endemic Plants from Mauritius.

The chemical and pharmacological profiles of essential oils (EOs) hydrodistilled in yields of 0.03-0.77 % (w/w) from three exotic (Cinnamomum camphora, Petroselinum crispum, and Syzygium samarangense) and two endemic (Pittosporum senacia subsp. senacia and Syzygium coriaceum) medicinal plants were studied. GC-MS/GC-FID analysis of the EOs identified the most dominant components to be myristicin (40.3 %), myrcene (62.2 %), 1,8-cineole (54.0 %), β-pinene (21.3 %) and (E)-β-ocimene (24.4 %) in P. crispum, P. senacia and C. camphora, S. samarangense and S. coriaceum EOs, respectively. All EOs were found to possess anti-amylase (0.70-1.50 mM ACAE/g EO) and anti-tyrosinase (109.35-158.23 mg KAE/g) properties, whereas no glucosidase inhibition was displayed. Only Syzygium EOs acted as dual inhibitors of both acetyl- and butyryl-cholinesterases, while P. senacia and C. camphora EOs inhibited acetylcholinesterase selectively and P. crispum EO was inactive (AChE: 4.64-4.96 mg GALAE/g; BChE: 5.96 and 7.10 mg GALAE/g). Molecular docking revealed 1,8-cineole to present the best binding affinities with butyrylcholinesterase, amylase and tyrosinase, while both myristicin and β-pinene with acetylcholinesterase and finally β-pinene with glucosidase. In vitro antioxidant potency was also demonstrated in different assays (DPPH: 13.52-53.91 mg TE/g, ABTS: 5.49-75.62 mg TE/g; CUPRAC: 45.38-243.21 mg TE/g, FRAP: 42.49-110.64 mg TE/g; and phosphomolybdenum assay: 82.61-160.93 mM TE/g). Principal component analysis revealed the EOs to differ greatly in their bioactivities due to their chemodiversity. This study has unveiled some interesting preliminary pharmacological profiles of the EOs that could be explored for their potential applications as phytotherapeutics.

Ultrasound Extraction Mediated Recovery of Nutrients and Antioxidant Bioactive Compounds from Phaeodactylum tricornutum Microalgae

In recent years, a growing interest has been shown in the use of microalgae due to their interesting nutritional and bioactive profiles. Green innovative processing technologies such as ultrasound-assisted extraction (UAE) avoid the use of toxic solvents and high temperatures, being a sustainable alternative in comparison with traditional extraction methods. The present study aims to evaluate the recovery of high added-value compounds from Phaedoactylum tricornutum assisted by ultrasound. To optimize the UAE of proteins, carbohydrates, pigments and antioxidant compounds, a response surface methodology was used. Carbohydrate extraction was positively affected by the temperature. However, for the extraction of carotenoids, the most influential factor was the extraction time. The total polyphenols were only significantly affected by the extraction time. Finally, the antioxidant capacity, measured by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was strongly modulated by the extraction time, while for the oxygen radical antioxidant capacity (ORAC) assay, the most important parameter was the temperature, followed by the extraction time. The optimal conditions for the maximum extraction of nutrients, bioactive compounds and antioxidant capacity were 30 min, 50 ºC and a pH of 8.5. Finally, it has been seen that with these conditions, the extraction of fucoxanthin is allowed, although no differences were found between an ultrasound-assisted extraction and a shaking extraction (control).

Multitargeting application of proline-derived peptidomimetics addressing cancer-related human matrix metalloproteinase 9 and carbonic anhydrase II.

A series of d-proline peptidomimetics were evaluated as dual inhibitors of both human carbonic anhydrases (hCAs) and human gelatinases (MMP2 and MMP9), as these enzymes are both involved in the carcinogenesis and tumor invasion processes. The synthesis and enzyme inhibition kinetics of d-proline derivatives containing a biphenyl sulfonamido moiety revealed an interesting inhibition profile of compound XIV towards MMP9 and CAII. The SAR analysis and docking studies revealed a stringent requirement of a trans geometry for the two arylsulfonyl moieties, which are both necessary for inhibition of MMP9 and CAII. As MMP9 and CAII enzymes are both overexpressed in gastrointestinal stromal tumor cells, this molecule may represent an interesting chemical probe for a multitargeting approach on gastric and colorectal cancer.

Modulation of carotenoid/flavonoid profiles and sugar content of a potential functional citrus-based food through crossflow microfiltration

Crossflow microfiltration was implemented to enrich a clementine/pink grapefruit juice in carotenoids, flavonoids and pectins modulating its sugar content thanks to an added diafiltration step. Using tubular ceramic membrane with pore diameter of 0.2 μm at 30–40 °C, a crossflow velocity of 5 m‧s−1 and a transmembrane pressure of 2.6 bar, the impact of the process was assessed focusing on bioactive compounds and physical characteristics of the concentrates. With permeate flux above 30 kg‧h−1‧m−2, the process resulted in an increase of 8–10 times in the content of provitamin A carotenoids (33 mg‧kg−1 in the final products), lycopene (43 mg‧kg−1), hesperidin (3.2 g‧kg−1) and pectin (5.5 g‧kg−1) giving a unique and interesting composition profile. Moreover, diafiltration divided the sugar content by 3. This can be relevant for the product to be recommended as part of a reduced sugar diet for diabetic people. By modelling the retention of bioactive compounds, we showed that it was possible to modulate sugar, flavonoids, carotenoids and pectin contents in the concentrate that could be a key issue for the biological activity of this potential functional citrus-based food.

Development of Chinese chestnut whiskey: yeast strains isolation, fermentation system optimization, and scale-up fermentation

In this study, we used Chinese chestnut as the main raw material to develop a novel type of whiskey. First, 16 yeasts were isolated and identified for producing aroma using olfactory plate assay. Of these, we screened nine yeast strains based on their fermentation capacity, aroma profile, and sensory evaluation. The results demonstrated the combination of strains HN006 (Saccharomyces cerevisiae) and HN010 (Wickerhamomyces anomalus) provided satisfactory wine fermentation with an interesting flavor profile, as strain HN010 was highly aromatic and had elevated sensory scores with comparatively low ethanol yield, while strain HN006 had a poor flavor profile but produced the largest amount of ethanol. Subsequently, we co-cultured strains HN006 and HN010 to optimize the fermentation system. The results revealed the following optimum parameters: a mixed inoculum of 6% (v/v) at an HN006/HN010 ratio of 1:2 (v/v), a raw material ratio of 5:3:2 (chestnut: malt: glutinous rice), and yeast extract concentration of 6 g/L. Additionally, this fermentation system was successfully scaled-up to a 1000 L pilot-scale system. The results of this study showed that strains HN006 and HN010 could be used as alternatives for whiskey fermentation, as well as provided a generalized experimental scheme to assess other microorganisms.

Preferences and Orientations of Social Work Students towards Methods and Forms of Professional Practice

This article presents results from research on preferences of social work students (n=60) towards particular methods and forms when practicing their profession. A conceptual model has been applied, where measurements and analysis lead to identification of two methodological orientations: micro- and macro-orientation. Based on research data conclussions are made about the profile of the students' interests for practicing the social work profession in particular areas and settings. Presented analysis concludes that social work education prepares professionals with profiles relevant to the needs of social work practice.

BLE-Based Indoor Tracking System with Overlapping-Resistant IoT Solution for Tourism Applications.

In this paper, an overlapping-resistant Internet of Things (IoT) solution for a Bluetooth Low Energy (BLE)-based indoor tracking system (BLE-ITS) is presented. The BLE-ITS is a promising, inexpensive alternative to the well-known GPS. It can be used in human traffic analysis, such as indoor tourist facilities. Tourists or other customers are tagged by a unique MAC address assigned to a simple and energy-saving BLE beacon emitter. Their location is determined by a distributed and scalable network of popular Raspberry Pi microcomputers equipped with BLE and WiFi/Ethernet modules. Only simple triggered messages in the form of login records (LRs) are sent to a server, where the so-called path vectors (PVs) and interest profile (IPr) are set. The authors implemented the prototype and demonstrated its usefulness in a controlled environment. As it is shown in the paper, the solution is highly overlap-resistant and mitigates the so-called multilocation problem.

Interest, Burnout, and Drop-Out Intentions Among Finnish and Danish Humanities and Social Sciences PhD. Students

Aim/Purpose: This study focused on advancing understanding of individual variations in doctoral students’ interest in their doctoral studies and how they related to experiences of burnout and drop-out intentions in Denmark and Finland. Background: Ph.D. students’ experiences of interest, burnout, and dropout intentions among Finnish and Danish Ph.D. students have not been researched before. Research with a person-centred approach exploring individual variations in students undertaking doctoral studies in two comparable but distinct socio-cultural contexts is limited. Methodology: This study uses exploratory factor analysis, K-means cluster analyses in combination with Pairwise comparisons, ANOVA, and Chi-square test. A total of 365 doctoral students in social sciences and humanities disciplines in Finland and Denmark responded to a Cross-Cultural Doctoral Experience Survey. Contribution: This study contributes understanding on individual variation in doctoral students’ interest across two socio-cultural contexts by identifying four personal interest profiles. The profiles were invariant across the contexts. The study also shed further light on the interrelation between the interest in research and the risk for suffering from burnout and entertaining dropout intentions. Findings: The interest profiles identified among the Ph.D. students were the High interest profile, the Moderate interest profile, the Developmental, research and impact interest profile, and the Development and impact interest profile. All interest profiles exhibited high levels of the developmental interest, however they varied especially in the weight given to instrumental and research interests. Ph.D. students in the Moderate interest profile showed signs of burnout, and they were prone to consider dropping out. Also, individuals in the Development and impact interest profile considered more frequently dropping out of their studies. Recommendations for Practitioners: Investing in the identification and support of interest among Ph.D. students is worthwhile, as interest is not a permanent characteristic of the individual, and the combination of research, development, and impact interest indicates a lower risk for burnout and drop-out intentions. Recommendation for Researchers: It is possible that interest profiles are the same across the two national contexts investigated in this study, but their underpinnings and premises are different. It is likely that a qualitative approach would shed more light on these foci. Impact on Society: The results imply that personal interest was not determined by the socio-cultural differences between the countries, indicating that cultivating doctoral students’ personal interest, particularly a combination of research, development, and impact, provides a potential buffer for doctoral students’ burnout and drop-out, which has been raised as global concerns among policy makers, researchers, and doctoral education developers and administrators during the past decade. The study has impact on doctoral studies in international communities. Future Research: The results in this study reflect specific characteristics of social sciences and their applied nature. It remains for future research to investigate the extent to which the identified four profiles of interest in relation to burnout and drop-out intentions emerge in the natural sciences.

Latent profiles of the vocational interests from students of vocational education and engineering disciplines

The lack of teachers in vocational education, especially in technical subjects, is a persistent problem. Based on the career choice theory according to Holland (1997), this paper analyses with latent variable mixture modeling (LVMM) the characteristic vocational interest profiles of prospective teachers in technical subjects and compares them with the interest profiles of students in engineering disciplines. The results show that (1) a large percentage of prospective teachers have an undifferentiated interest profile (2) the interest profiles of students are associated with the choice of study course and (3) correspond to the specific characteristics of the study courses.