<h3>Purpose/Objective(s)</h3> Lesbian, gay, bisexual, transgender, queer/questioning, Two-Spirit, plus (LGBTQ2+) individuals experience cancer-related health disparities and inequities. Our objective was to examine healthcare professionals' (HCPs) knowledge, attitudes, practices and education interest when caring for LGBTQ2+ individuals with cancer, and to identify gaps and opportunities to improve care. <h3>Materials/Methods</h3> A 38-item electronic survey was sent to all Gynecologic oncology staff (<i>n</i>=92) within a tertiary care cancer center in Toronto. Items included respondent demographics (<i>n</i>=7), and LGBTQ2+ knowledge (<i>n</i>=7), attitudes (<i>n</i>=15), practice behaviors <i>(n</i>=5) and education interest (n=1). There were 3 open-ended items exploring personal experiences, reservations, and suggestions for improving LGBTQ2+ care. Knowledge and attitude items were anchored on a 5-point Likert scale with additional options for not sure and prefer not to answer. Practice behaviors and education interest items were measured using categorical response options. Descriptive statistics summarized survey responses. Thematic analysis was used to analyze open-ended questions. <h3>Results</h3> 75/92 HCPs completed the survey (82% response rate). Almost all respondents reported comfort (87-96%; strongly agree/agree) treating LGBTQ2+ individuals, most reported a lack of knowledge about LGBTQ2+ health needs (55-73%). Respondents strongly agreed/agreed it was important to know a patient's sexual orientation and gender identity, while less respondents strongly agreed/agreed that they felt comfortable asking patients (sexual orientation 58% vs 41% p=0.023 and gender identity 82% vs 36% p <0.001). Almost all (96% strongly agreed/agreed) were interested in receiving LGBTQ2+ specific education. Two main themes were identified: (i) HCPs experience fear of offending LGBTQ2+ individuals because of their lack of knowledge, and (ii) HCPs desire LGBTQ2+-specific health training, especially in asking a patient's pronouns, sexual orientation, gender identity, and in caring for transgender patients. <h3>Conclusion</h3> Although HCPs report feeling comfortable caring for LGBTQ2+ individuals, most report a lack of knowledge and awareness in caring for this population. We recommend institutions implement cultural competency training for HCPs to improve inclusive patient-centered care.
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