Isometric exercise (IE), including wall squat training, has been shown to be effective at reducing resting blood pressure (BP). Rating of perceived exertion (RPE) is also widely used as an accessible additional measure of IE intensity. Despite this, no RPE scales have been specifically designed for use with IE and it is not clear whether RPE is sensitive enough to distinguish between different lower limb IE workloads. Therefore, the aims of this study were to assess the validity and reliability of RPE as a measure of IE intensity (workload) and physiological exertion (Heart rate and BP), and to examine whether RPE is able to discern differences in wall squat workload (knee angle) at a resolution of 10-degrees, as was previous shown for heart rate (HR) and BP. Twenty-nine male participants completed eight separate isometric wall squat testing sessions, separated by a minimum of 5-h. Each session consisted of a single 2-min isometric wall squat test, at one of five randomized workloads (knee joint angles). Three of the knee angles were repeated, a second time, to allow measurements of reliability. Throughout the exercise protocol, HR and BP were recorded continuously; values for each 30-s time-point were calculated as the mean of the proceeding 5-s, and peak values for the 2-min bout were taken as the mean results for the final 5-s of the bout. In addition, mean results for the full 2-min period were calculated. RPE was collected every 30 s. Concurrent validity was assessed by correlating RPE results with the criterion measures: Knee joint angle, HR, and BP. Differences in RPE were assessed across consecutive workloads and time-points. There were significant increases in RPE at each consecutive wall squat workload (P<0.001) and between each consecutive 30-s time point (P<0.001). In addition, the RPE results produced a significant inverse relationship with knee angle (r=-0.79; P<0.001) and significant positive relationships with HR (r=0.53, P<0.001) and BP (systolic: r=0.77; diastolic: r=0.62; and mean arterial pressure: r=0.70, P<0.001). RPE provides a valid and reliable measure of isometric wall squat intensity, physiological exertion and can discern between knee angles with a resolution of 10°. Patients and practitioners implementing isometric exercise training for arterial blood pressure reduction can use RPE to accurately monitor the intensity of the exercise and the physiological responses.