This study proposes an experimental model to analyze homeopathic potencies' biological and physicochemical effects on intense magnetic fields. The proposed model is based on the exposition of Artemia franciscana cysts to saxitoxin (cyanobacteria extract) and the treatment with R. raciborskii extract isotherapic 200cH (named saxitoxin 200cH), followed by the hatching and nauplii (larvae) vitality rate (live nauplii/total cysts), and the gene expression of heat shock proteins (HSP 26, 40, 90, and p26), as previously described ad bio-resilience indicators [1,2]. In a second experiment, pH, conductivity, and temperature temporal curves were built, and different treatments were compared before and after being poured into the seawater. The method details are as follows: Artemia franciscana cysts were seeded into artificial seawater in 96-well plates in triplicate, exposed immediately to 25ppm of R. raciborskii extract, and treated with sterile purified water (SPW), succussed SPW (vehicle controls), or saxitoxin 200cH prepared in SPW, submitted or not to a 2400 Gauss static magnetic field for 15 minutes. Plates were kept in a Faraday cage under stable lightening (300 mini-led lamps), temperature (26.5%), humidity (65%), and magnetic field (0.7 µT). The hatching and vitality rate were analyzed after 48 hours. In the second experiment, the physicochemical parameters were analyzed before and after one minute, two, and 24 hours after pouring the samples into seawater (100µL/100mL), kept at the same environmental conditions set for the first experiment. The statistical analysis was made by two-way ANOVA / Tukey, with α=0.05. Treatment of cysts with SPW alone reduced the hatching and nauplii vitality rate in relation to the other treatments (p≤0.05); however, the treatment with saxitoxin 200cH exhibited smaller variance and the highest statistical hatching (p=0.0009) and vitality (p=0.0007) levels. The previous submission of samples to the magnetic field reverted this result by canceling the statistical differences and increasing the variance of saxitoxin 200cH datapoints. The analyses of HSP gene expression are still in course. In the second experiment, there is no specific effect of saxitoxin 200cH on temperature, conductivity, and pH, independent of the exposition of samples to the magnetic field. The results corroborate the hypothesis that a possible interference on dipolar dynamics of saxitoxin 200cH caused by the intense magnetic field could negatively interfere with its biological effect independently of eventual changes in temperature, conductivity, and pH in the seawater, although its clear capacity to improve tracking homeopathic potencies in water when using the method of solvatochromic dyes, as previously shown [3].
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