Teaching professionals are crucial for the successful realization of e-Learning in school education. Major Asian cities/regions such as Singapore, Hong Kong, Taiwan, and Beijing continuously invest in teacher development on e-Learning to empower frontline teachers and school leaders to advance school education in the digital era. This article aims to review and discuss the activity contents, delivery approaches, and scaling-up methods of teacher development on e-Learning in these four major Asian cities/regions. There is a unique trend identified in each of the four major Asian cities/regions. Singapore focuses on professional peer-learning communities for supporting practice-based teacher development on the effective use of ICT for learning and teaching in the classroom. Hong Kong focuses on building teacher communities for observing, engaging, and reflecting and succeeding school-based e-Learning initiatives for student-centered learning. Taiwan focuses on offering university-supported teacher development on the effective integration of e-Learning into students’ learning, especially for learning twenty-first century skills. Beijing focuses on promoting self-directed teacher development on the advancement of ICT infusion in classroom teaching, especially the ICT-supported innovative pedagogies for K-12. Implications are drawn in order to provide a point of reference for other cities/regions in tailoring the future provision of teacher development for e-Learning in the twenty-first century.