Data Integration Research Articles

Current and future applications of local field potential-guided programming for Parkinson's disease with the Percept™ rechargeable neurostimulator.

Deep brain stimulation (DBS) has been established as an effective neuromodulatory treatment for Parkinson's disease (PD) with motor complications or refractory tremor. Various DBS devices with unique technology platforms are commercially available and deliver continuous, open-loop stimulation. The Percept™ family of neurostimulators use BrainSense™ technology with five key features to sense local field potentials while stimulating, enabling integration of physiologic data into the routine practice of DBS programming. The newly approved Percept™ rechargeable RC implantable pulse generator offers a smaller, thinner design and reduced recharge time with prolonged recharge interval. In this review, we describe the application of local field potential sensing-based programming in PD and highlight the potential future clinical implementation of closed-loop stimulation using the Percept™ RC implantable pulse generator.

Optimizing data management in healthcare: Lessons from clinical trials and beyond

This study comprehensively explores the optimization of data management in healthcare by drawing on lessons learned from clinical trials and extending these insights to broader healthcare contexts. The study's purpose is to examine the challenges, strategies, and technological innovations that are essential for effective data management in modern healthcare systems. Utilizing a detailed literature review, the study identifies key methodologies from clinical trials that have successfully enhanced data integrity, accuracy, and security, such as the implementation of electronic data capture (EDC) systems and rigorous data governance frameworks. These methods are crucial in managing the increasing volume and complexity of healthcare data. The study's findings reveal that big data analytics and artificial intelligence (AI) are pivotal in transforming healthcare data management, enabling personalized and precise patient care. However, these technologies also introduce challenges related to data security, interoperability, and ethical considerations, particularly concerning patient privacy and the transparency of AI-driven decision-making processes. In conclusion, the study emphasizes the need for continuous innovation and improvement in healthcare data management practices. Recommendations include the integration of blockchain technology, cloud computing, and real-time data from wearable devices to address existing challenges and enhance data management capabilities. The study advocates for a balanced approach that prioritizes patient privacy, ethical governance, and regulatory compliance to ensure that technological advancements contribute positively to patient outcomes and healthcare efficiency.

A Survey on Classifying Ocular Diseases Using Deep Learning and Machine Learning Techniques

In ophthalmology, using fundus images to identify ocular diseases early may pose challenges for clinicians. Manually diagnosing ocular conditions is time-consuming, difficult, and requires experimentation. As a result, technology was created to help computers differentiate between ocular diseases. It is possible to create a system of this type due to different learning algorithms based on visual capabilities. Recent breakthroughs in deep learning and machine learning have led to the development of intelligent systems that improve accuracy and efficiency in classifying eye diseases. The purpose of this study is to conduct a comprehensive survey of modern systems that classify ocular problems using different methods, including pre-trained deep learning networks, using the Ocular Disease Intelligent Recognition (ODIR) dataset. The goal is to build and train a model that can recognize and classify ocular disorders. Previous research indicates the increasing use of deep learning techniques. CNN-based methods have spread widely in this field, compared to traditional manual procedures, due to their outstanding results. The most prominent deep learning techniques are convolutional neural networks (CNNs), recurrent neural networks (RNNs), and various learning methods for increasing and transferring data. The survey highlights the potential of these systems to enhance classification accuracy and sensitivity while addressing challenges such as data availability, interpretability, and integration with clinical practice.

Mapping data-driven strategies in improving health care and patient satisfaction

Health services involve data analytics, central to healthcare service delivery, research, and planning. Based on the literature, this specific article seeks to enlighten the reader about data analytics in health services by discussing its advantages, shortcomings, and issues that come with it. Section one mapping outlines how data analytics optimizes the results of health service delivery through risk factor definition, disease detection, and case-by-case management. It helps inform disease trajectory, determine drug interaction, monitor disease, support decision-making, and enhance service delivery quality. The research also examines data analysis in health services, medicine, and pharmaceuticals. Several big data analytics applications have been identified as relevant to drug development, personalized medication, and specialized trials. Evaluations that rely on biomarkers differentiate between patients who are convenient for specific therapies, improving the patient's status and developing health care services. The third section also stresses the role of data analytics in enhancing efficiency and profitability. They involve detecting fraud, abuse, and unnecessary medical activities, creating savings and better financial returns. Data analytics also leads to identifying high-cost patients and ways of managing healthcare costs, increasing revenues and profitability. The fourth section discusses how big data complements traditional public health surveillance and outbreak platforms. It facilitates collecting data, analyzing different sources, and identifying special patterns or outliers to detect the outbreak early enough, contain it, or allocate resources to control it. It anticipates and addresses the dangers to the public's well-being and effectively controls episodes of infectious diseases. However, some key issues are associated with data analytics implementation, such as data quality, governance, privacy, bias, integration, expertise, and ethics, among others. Therefore, solving these challenges to achieve the optimum shifting of health service paradigms through data analytics in inpatient treatment, organizational management, and affordability is imperative.

The impact of artificial intelligence on regulatory compliance in the oil and gas industry

Artificial Intelligence (AI) is increasingly transforming the regulatory compliance landscape in the oil and gas industry. This paper examines the profound impact of AI on ensuring adherence to complex regulatory frameworks governing this sector. Regulatory compliance in the oil and gas industry involves adhering to a myriad of environmental, safety, and operational regulations, often posing significant challenges due to the volume and complexity of data involved. AI technologies, including machine learning, natural language processing, and predictive analytics, offer innovative solutions to these challenges. AI enhances data management and analysis by automating data collection, processing, and reporting, thereby increasing accuracy and efficiency. Predictive maintenance and risk assessment tools powered by AI can identify potential compliance issues before they arise, allowing for proactive measures. Moreover, AI-driven compliance monitoring systems enable real-time tracking of regulatory adherence, reducing the risk of non-compliance and associated penalties. Automated auditing and inspection processes further streamline compliance checks, ensuring thorough and consistent evaluations. Case studies demonstrate successful AI implementations in regulatory compliance, such as automated reporting systems in offshore drilling and predictive maintenance in pipeline management, which have resulted in improved compliance rates and reduced operational risks. However, the adoption of AI is not without challenges. Issues related to data quality and integration, cybersecurity, and regulatory acceptance pose significant hurdles. Additionally, ethical and legal considerations surrounding AI deployment must be addressed to ensure responsible use. AI holds substantial potential to revolutionize regulatory compliance in the oil and gas industry by enhancing efficiency, accuracy, and proactive risk management. As AI technologies continue to evolve, their integration into compliance processes will likely become more sophisticated, offering greater benefits and addressing current limitations. The future of regulatory compliance in the oil and gas sector will be increasingly shaped by the advancements in AI, driving both operational excellence and adherence to stringent regulatory standards.

Enhancing severe hypoglycemia prediction in type 2 diabetes mellitus through multi-view co-training machine learning model for imbalanced dataset

Patients with type 2 diabetes mellitus (T2DM) who have severe hypoglycemia (SH) poses a considerable risk of long-term death, especially among the elderly, demanding urgent medical attention. Accurate prediction of SH remains challenging due to its multifaced nature, contributed from factors such as medications, lifestyle choices, and metabolic measurements. In this study, we propose a systematic approach to improve the robustness and accuracy of SH predictions using machine learning models, guided by clinical feature selection. Our focus is on developing long-term SH prediction models using both semi-supervised learning and supervised learning algorithms. Using the action to control cardiovascular risk in diabetes trial, which includes electronic health records for over 10,000 individuals, we focus on studying adults with T2DM. Our results indicate that the application of a multi-view co-training method, incorporating the random forest algorithm, improves the specificity of SH prediction, while the same setup with Naive Bayes replacing random forest demonstrates better sensitivity. Our framework also provides interpretability of machine learning models by identifying key predictors for hypoglycemia, including fasting plasma glucose, hemoglobin A1c, general diabetes education, and NPH or L insulins. The integration of data routinely available in electronic health records significantly enhances our model’s capability to predict SH events, showcasing its potential to transform clinical practice by facilitating early interventions and optimizing patient management. By enhancing prediction accuracy and identifying crucial predictive features, our study contributes to advancing the understanding and management of hypoglycemia in this population.

Mapping the landscape: A systematic literature review on automated valuation models and strategic applications in real estate

In the rapidly evolving real estate industry, integrating automated valuation models (AVMs) has become critical for improving property assessment accuracy and transparency. Although there is some research on the subject, no thorough qualitative systematic review has been done in this field. This paper aims to provide an up-to-date and systematic understanding of the strategic applications of AVMs across various real estate subsectors (i.e., real estate development, real estate investment, land administration, and taxation), shedding light on their broad contributions to value enhancement, decision-making, and market insights. The systematic review is based on 97 papers selected out of 652 search results with an application of the PRISMA-based method. The findings highlight the transformative role of AVMs approaches in streamlining valuation processes, enhancing market efficiency, and supporting data-driven decision-making in the real estate industry, along with developing an original conceptual framework. Key areas of future research, including data integration, ethical implications, and the development of hybrid AVMs approaches are identified to advance the field and address emerging challenges. Ultimately, stakeholders can create new avenues for real estate valuation efficiency, accuracy, and transparency by judiciously utilizing AVMs approaches, leading to more educated real estate investment decisions.

포스트모던 박물관 시대에 인터랙티브 미디어를 활용한 무형 문화유산 살아있는 전시 방안 연구

This study addresses challenges in the living exhibitions of intangible cultural heritage through interactive media, providing curators with clear design strategies. It outlines the characteristics of these exhibitions and the technical features of interactive media, summarizes the difficulties encountered, and proposes effective solutions. The results show that cross-platform data integration and cross-industry collaboration help curators accurately distinguish between the appearance and essence of living exhibitions. The development of mobile communication technology is transforming how interactive media is presented, and the integration of 5G provides stable support for dynamic displays across time and space. Participatory audience interaction platforms expand engagement channels and fundamentally shift the unequal relationship between museums and visitors.

A Comparative Analysis of Microsoft Fabric and Snowflake: Architectural Paradigms and Performance Metrics in Modern Cloud Data Platforms

Abstract: This article presents a comprehensive comparative analysis of two leading cloud data platforms: Microsoft Fabric and Snowflake. As organizations increasingly rely on cloud-based solutions for data management and analytics, understanding the strengths and limitations of these platforms becomes crucial. This article examines the architectural foundations, functional capabilities, performance metrics, and cost considerations of both platforms. We explore Microsoft Fabric's integrated service model within the Azure ecosystem and Snowflake's multi-cluster shared data architecture, assessing their approaches to data integration, storage, analytics, and machine learning integration. Through empirical performance comparisons and an evaluation of scalability mechanisms, we provide insights into the operational efficiencies of each platform. Additionally, we analyze the security features, compliance standards, and pricing models to offer a holistic view of the total cost of ownership. Our findings reveal distinct advantages in Microsoft Fabric's end-to-end integration and Snowflake's performance in multicloud environments, while also highlighting areas for potential improvement in both platforms. This article aims to serve as a valuable resource for organizations navigating the complex landscape of cloud data solutions, offering evidence-based criteria for platform selection aligned with specific business needs and technological ecosystems

Data-driven decision-making in public health: The role of advanced statistical models in epidemiology

This paper critically examines the transformative role of data-driven decision-making in public health, focusing on the integration of advanced statistical models in epidemiology. As the volume and complexity of health data increase, leveraging predictive analytics, machine learning, and real-time data integration has become essential for improving public health outcomes. The study explores how these technologies have shifted public health strategies from reactive to proactive approaches, particularly in areas such as disease surveillance, chronic disease management, and health equity. Through comprehensive analysis, the paper identifies key advancements, such as hybrid models that combine traditional epidemiological frameworks with AI, and the integration of multi-modal data sources that enhance predictive accuracy. The findings emphasize the potential of these models to optimize resource allocation, address health disparities, and provide timely interventions. However, challenges such as data quality, algorithmic bias, and the ethical implications of model transparency are highlighted as critical issues requiring ongoing research. The study concludes that for these models to be effectively adopted, there must be a balance between technological innovation and ethical considerations. Recommendations include the need for interdisciplinary collaboration, improved data governance frameworks, and the development of more inclusive models that are generalizable across diverse populations. This research underscores the necessity of combining robust analytical tools with ethical frameworks to enhance the reliability and equity of public health interventions.

Java Development across Industries: Transforming Healthcare, Retail, and Agriculture

Java, a versatile and widely-adopted programming language, is transforming critical industries such as healthcare, retail, and agriculture. This article explores how Java developers leverage the language's robustness, platform independence, and extensive ecosystem to create innovative solutions that address industry-specific challenges. In healthcare, Java's security features and scalability enable the development of secure and interoperable Electronic Health Record systems. Retail benefits from Java's cross-platform compatibility and performance in building omnichannel platforms that integrate online and offline experiences. In agriculture, Java's IoT capabilities and big data integration revolutionize farming practices through precision agriculture and data-driven decision-making. Case studies in each industry demonstrate Java's significant impact on key metrics such as patient data accessibility, online-to-offline conversions, and crop yields. As these sectors continue to evolve, Java's adaptability positions it as a key driver of future advancements.

Intelligent Prediction of Rate of Penetration Using Mechanism-Data Fusion and Transfer Learning

Rate of penetration (ROP) is crucial for evaluating drilling efficiency, with accurate prediction essential for enhancing performance and optimizing parameters. In practice, complex and variable downhole environments pose significant challenges for mechanistic ROP equations, resulting in prediction difficulties and low accuracy. Recently, data-driven machine learning models have been widely applied to ROP prediction. However, these models often lack mechanistic constraints, limiting their performance to specific conditions and reducing their real-world applicability. Additionally, geological variability across wells further hinders the transferability of conventional intelligent models. Thus, combining mechanistic knowledge with intelligent models and enhancing model stability and transferability are key challenges in ROP prediction research. To address these challenges, this paper proposes a Mechanism-Data Fusion and Transfer Learning method to construct an intelligent prediction model for ROP, achieving accurate ROP predictions. A multilayer perceptron (MLP) was selected as the base model, and training was performed using data from neighboring wells and partial data from the target well. The Two-stage TrAdaBoost.R2 algorithm was employed to enhance model transferability. Additionally, drilling mechanistic knowledge was incorporated into the model’s loss function as a constraint to achieve a fusion of mechanistic knowledge and data-driven approaches. Using MAPE as the measure of accuracy, compared with conventional intelligent models, the proposed ROP prediction model improved prediction accuracy on the target well by 64.51%. The model transfer method proposed in this paper has a field test accuracy of 89.71% in an oilfield in China. These results demonstrate the effectiveness and feasibility of the proposed transfer learning method and mechanistic–data integration approach.

Automated single-cell omics end-to-end framework with data-driven batch inference.

To facilitate single-cell multi-omics analysis and improve reproducibility, we present single-cell pipeline for end-to-end data integration (SPEEDI), a fully automated end-to-end framework for batch inference, data integration, and cell-type labeling. SPEEDI introduces data-driven batch inference and transforms the often heterogeneous data matrices obtained from different samples into a uniformly annotated and integrated dataset. Without requiring user input, it automatically selects parameters and executes pre-processing, sample integration, and cell-type mapping. It can also perform downstream analyses of differential signals between treatment conditions and gene functional modules. SPEEDI's data-driven batch-inference method works with widely used integration and cell-typing tools. By developing data-driven batch inference, providing full end-to-end automation, and eliminating parameter selection, SPEEDI improves reproducibility and lowers the barrier to obtaining biological insight from these valuable single-cell datasets. The SPEEDI interactive web application can be accessed at https://speedi.princeton.edu/. A record of this paper's transparent peer review process is included in the supplemental information.

Dynamic airspace sectorization with machine learning enhanced workload prediction and clustering

Addressing the complexities of modern Air Traffic Management (ATM), this paper introduces a novel framework for dynamic airspace sectorization, tailored to enhance efficiency and safety in congested airspaces. Central to this framework is the WP-ConvLSTM model, an innovative deep learning approach equipped with attention mechanisms. This model excels in accurately predicting workload dynamics, a critical factor in managing air traffic flow. To implement sectorization, we adopt a constrained K-means clustering technique for spatial division, followed by a refinement process involving Support Vector Machine (SVM) algorithms for precise boundary generation. Further optimization of sector boundaries is achieved through an evolutionary algorithm, ensuring both flexibility and stability in airspace divisions. Our methodology was thoroughly evaluated using real-world data from one of the busiest airspaces, demonstrating significant improvements in workload prediction accuracy and airspace sector management. The findings highlight the model’s robustness in practical scenarios, offering a scalable solution for ATM challenges. We conclude with a recognition of the study’s limitations and propose avenues for future research to build upon our findings, particularly in enhancing real-time data integration and adapting to evolving air traffic patterns.

A multi-step calibration strategy for reliable parameter determination of salt rock mechanics constitutive models

The storage of renewable hydrogen in salt caverns requires fast injection and production rates to cope with the imbalance between energy production and consumption. This raises concerns about the mechanical stability of salt caverns under such operational conditions. The use of appropriate constitutive models for salt mechanics is an important step in investigating this issue, therefore many constitutive models with several parameters have been presented in the literature. However, a robust calibration strategy to reliably determine which model and parameter set represents the given rock, based on stress–strain data sets, remains an unsolved challenge. In this paper, for the first time in the community, we present a multi-step strategy to determine a single parameter set based on many deformation data sets for salt rocks. Towards this end, we first develop a comprehensive constitutive model able to capture all relevant nonlinear deformation physics of transient, reverse, and steady-state creep. The determination of the single set of representative material parameters is then achieved by framing the calibration process as an optimization problem, for which the global Particle Swarm Optimization algorithm is employed. To allow for dynamic data integration, a multi-step calibration strategy is developed for a situation where experiments are included one at a time, as they become available. Additionally, due to the existing mild heterogeneity in the experimental rock samples, our optimization strategy is made flexible to allow for this slight heterogeneity. The devised optimization strategy, based on the multi-physics comprehensive constitutive modeling framework, results in a single set of representative material properties of all the deformation data sets. As a rigorous mathematical analysis for the presented method and the lack of relevant experimental data sets, we consider a wide range of synthetic experimental data sets, inspired by the existing sparse relevant data in the literature. The results of our performance analyses show that the proposed calibration strategy is robust. Moreover, the results become increasingly more accurate as more data sets become available.

Integration of scHi-C and scRNA-seq data defines distinct 3D-regulated and biological-context dependent cell subpopulations

An integration of 3D chromatin structure and gene expression at single-cell resolution has yet been demonstrated. Here, we develop a computational method, a multiomic data integration (MUDI) algorithm, which integrates scHi-C and scRNA-seq data to precisely define the 3D-regulated and biological-context dependent cell subpopulations or topologically integrated subpopulations (TISPs). We demonstrate its algorithmic utility on the publicly available and newly generated scHi-C and scRNA-seq data. We then test and apply MUDI in a breast cancer cell model system to demonstrate its biological-context dependent utility. We find the newly defined topologically conserved associating domain (CAD) is the characteristic single-cell 3D chromatin structure and better characterizes chromatin domains in single-cell resolution. We further identify 20 TISPs uniquely characterizing 3D-regulated breast cancer cellular states. We reveal two of TISPs are remarkably resemble to high cycling breast cancer persister cells and chromatin modifying enzymes might be functional regulators to drive the alteration of the 3D chromatin structures. Our comprehensive integration of scHi-C and scRNA-seq data in cancer cells at single-cell resolution provides mechanistic insights into 3D-regulated heterogeneity of developing drug-tolerant cancer cells.

WIMOAD: Weighted Integration of Multi-Omics data for Alzheimer's Disease (AD) Diagnosis.

As the most common subtype of dementia, Alzheimer's disease (AD) is characterized by a progressive decline in cognitive functions, especially in memory, thinking, and reasoning ability. Early diagnosis and interventions enable the implementation of measures to reduce or slow further regression of the disease, preventing individuals from severe brain function decline. The current framework of AD diagnosis depends on A/T/(N) biomarkers detection from cerebrospinal fluid or brain imaging data, which is invasive and expensive during the data acquisition process. Moreover, the pathophysiological changes of AD accumulate in amino acids, metabolism, neuroinflammation, etc., resulting in heterogeneity in newly registered patients. Recently, next generation sequencing (NGS) technologies have found to be a non-invasive, efficient and less-costly alternative on AD screening. However, most of existing studies rely on single omics only. To address these concerns, we introduce WIMOAD, a weighted integration of multi-omics data for AD diagnosis. WIMOAD synergistically leverages specialized classifiers for patients' paired gene expression and methylation data for multi-stage classification. The resulting scores were then stacked with MLP-based meta-models for performance improvement. The prediction results of two distinct meta-models were integrated with optimized weights for the final decision-making of the model, providing higher performance than using single omics only. Remarkably, WIMOAD achieves significantly higher performance than using single omics alone in the classification tasks. The model's overall performance also outperformed most existing approaches, highlighting its ability to effectively discern intricate patterns in multi-omics data and their correlations with clinical diagnosis results. In addition, WIMOAD also stands out as a biologically interpretable model by leveraging the SHapley Additive exPlanations (SHAP) to elucidate the contributions of each gene from each omics to the model output. We believe WIMOAD is a very promising tool for accurate AD diagnosis and effective biomarker discovery across different progression stages, which eventually will have consequential impacts on early treatment intervention and personalized therapy design on AD.

Budget impact analysis of high-priced orphan medicinal products intended for the treatment of rare diseases in China: evidence from a densely populated metropolis of Chengdu

BackgroundAn increasing number of orphan medicinal products (OMPs) are being included in social health insurance schemes, significantly improving access to medicines for patients with rare diseases. However, high-priced OMPs are still not covered, primarily due to health equity controversies and inadequate data systems required for economic evaluation. The aim of this study was to estimate the burden of drug expenditures and the size of the reimbursement budget required for high-priced OMPs from the perspectives of society and healthcare payers.MethodsThe study performed a budget impact analysis using data from multiple sources to estimate the reimbursement budget for high-priced OMPs in Chengdu, a densely populated metropolis in China. The budget analysis consisted of three main elements: the number of patients, the price of drugs, and the simulated policy scenario. By adjusting the combinations of these elements, the budget fluctuations for payers were estimated. Furthermore, the study predicted the budget trend for the next three years to validate its sustainability.ResultsThe analysis indicated that 98 rare disease patients in Chengdu required high-priced OMPs in 2019. This suggested a projected budget of CNY 179 million for these patients without reimbursement policies, from a societal perspective. Under six assumed policy scenarios, this budget ranged from CNY 32 million to CNY 156 million. Over the next three years, the annual budget was estimated to range from CNY 200 million to CNY 1.303 billion.ConclusionIntegration of multi-source data helps to obtain more scientifically reliable results on budget impacts. The study found that the budgetary impacts of high-priced OMPs on society and payers are relatively limited. Health policymakers can choose appropriate reimbursement strategies based on financial affordability among a diverse mix of elements. The results of related studies provided insights for optimizing the allocation of health resources and improving patient access to medications.

The temporal dynamics of the gut mycobiome and its association with cardiometabolic health in a nationwide cohort of 12,641 Chinese adults.

The dynamics of the gut mycobiome and its association with cardiometabolic health remain largely unexplored. Here, we employ internal transcribed spacer (ITS) sequencing to capture the gut mycobiome composition and dynamics within a nationwide human cohort of 12,641 Chinese participants, including 1,946 participants with repeated measurements across three years. We find that the gut mycobiome is associated with cardiometabolic diseases and related biomarkers in both cross-sectional and dynamic analyses. Fungal alpha diversity indices and 19 mycobiome genera are the major contributors to the mycobiome-cardiometabolic disease link. Particularly, Saccharomyces emerges as an effect modifier of traditional risk factors in promoting type 2 diabetes risk. Further integration of multi-omics data reveals key metabolites such as γ-linolenic acid and L-valine linking the gut mycobiome to type 2 diabetes. This study advances our understanding of the potential roles of the gut mycobiome in cardiometabolic health.

Explore the mechanism of mulberry leaf combined with gut microbiota to improve hyperlipidemia based on network pharmacology

PurposeTo elucidate the underlying mechanisms by which mulberry leaves, in conjunction with gut microbiota, ameliorate hyperlipidemia and identify key gut microbial taxa involved, a mulberry leaf/gut microbiota-active components-metabolites-target-pathway network was constructed.MethodologyInformation on active constituents of mulberry leaves, metabolites generated by gut microbiota, and target proteins associated with hyperlipidemia were retrieved from multiple databases. Employing bioinformatics tools, relevant pathways and targets involved in the synergistic effect of mulberry leaves and gut microbiota on hyperlipidemia improvement were systematically analyzed. Through multi-level data analysis and integration of existing knowledge on the interplay between mulberry leaves, gut microbiota, and hyperlipidemia, the atherosclerosis signaling pathway was identified as the central pathway, with AKT1 as the core target protein. Based on metabolites produced by gut microbiota that act on AKT1, 29 specific gut microbial strains were found to exhibit synergistic action within the context of mulberry leaf-mediated hyperlipidemia alleviation. This culminated in the establishment of a comprehensive mulberry leaf/gut microbiota-active components-metabolites-target-pathway network. Moreover, molecular docking and visualization analyses demonstrated stable interactions between the selected gut microbiota-derived metabolites and the central target AKT1.ConclusionThese findings provide a robust scientific basis for the application of mulberry leaf-based formulations combined with probiotics in the development of specialized medical foods targeting hyperlipidemia, grounded in the elucidated molecular mechanisms and identified key gut microbial players.