Integrative Approach Research Articles

Triangular Greenness Index Analysis for Monitoring Fungal Disease in Pine Trees: A UAV-based Approach

The Triangular Greenness Index (TGI) is a vegetation index derived from high-resolution aerial images acquired using unmanned aerial vehicles (UAVs). It serves as a valuable tool for quantifying vegetation health and dynamics in the visible spectrum. The TGI combines key components, including red reflectance and green reflectance, extracted from UAV-based imagery. The red component represents chlorophyll absorption and photosynthetic activity, while the green component reflects vegetation density and canopy structure. By integrating these components, the TGI offers a comprehensive measure of photosynthetically active vegetation, utilizing UAVs as a data collection platform. This study highlight the importance of the TGI derived from UAV-based imagery in monitoring vegetation changes, assessing ecosystem responses, and tracking variations in land cover and biodiversity. Furthermore, the application of TGI analysis using UAV-based aerial imagery shows promise in accurately identifying and monitoring vegetation affected by fungal diseases. This integrated approach enables the detection of diseased trees based on distinct changes in greenness observed in their foliage. Because fungal diseases dry the plant and cause the green areas to disappear. The integration of UAV technology enhances the accuracy and efficiency of TGI calculation, contributing to effective management and conservation strategies in the context of fungal disease detection in vegetation. In this study, TGI was produced using UAV-based orthophoto and healthy and sick trees were determined. According to the accuracy analysis, producer accuracy for detecting green plants was 99.7% and user accuracy was 98.5%. Fungal disease could be detected with 98.5% producer accuracy and 96.5% user accuracy. The overall accuracy of the study was calculated as 98.6%.

Risk management in the beverage production industry using FMEA and fuzzy cognitive map in an uncertain environment

Proper and systematic management of food industry failures can improve the quality of products and save a lot on the costs of organizations and people’s health. One of the conventional methods for risk assessment is the Failure Modes and Effects Analysis (FMEA) which is often performed in a phase or stage. Compared to the combined methods, this method is less accurate due to similar priorities of failure in the evaluation and the lack of consideration of the interaction between risks. The current research has applied an integrated approach based on two techniques, FMEA and Fuzzy Cognitive Map (FCM), in a multi-stage manner to increase assessment accuracy and ranking of failures. By considering the risks of an industry in an uncertain environment and the causal relationships between failures, this approach can evaluate the industry’s risks better than conventional methods. In the research method, the initial prioritization of failures by the FMEA method is used as the input of the multi-stage FCM. The cause-and-effect relationship between the failures is determined by experts and the functional records of the processes, and the FCM is prepared. Since no research evaluates the risks of the malting industry step by step and considers the causal relationships between the risks, the present study has improved risk evaluation in the malting industry by using a multi-stage FCM. The ranking results with the proposed hybrid approach and its comparison with the conventional methods showed that the rating became more accurate, and the multiple priorities were improved. Managers of the malt beverage industry can make effective investment decisions to reduce or better control the risks of this industry by using the results of applying the proposed approach.

Institutional constraints to social dialogue in work integration of persons with disabilities: Slovakia and Norway compared

PurposeStudies on the work integration of persons with disabilities (PwD) and the role of social dialogue therein are scarce. The study examines how the different systems of workers’ representation and industrial relations in Slovakia and Norway facilitate PwD work integration. Taking a social ecosystem perspective, we acknowledge the role of various stakeholders and their interactions in supporting PwD work integration. The paper’s conceptual contribution lies in including social dialogue actors in this ecosystem.Design/methodology/approachEvidence was collected via desk research, 35 semi-structured in-depth interviews with 51 respondents and stakeholder workshops in 2019–2020.FindingsThe findings from Norway confirm the expected coordination of unions and employers in PwD work integration. Evidence from Slovakia shows that in decentralised industrial relations systems, institutional constraints beyond the workplace determine employers’ and worker representatives’ approaches in PwD integration. Most policy-level outcomes are contested, as integration occurs predominantly via sheltered workplaces without interest representation.Social implicationsThis paper identifies the primary sources of variation in the work integration of PwD. It also highlights opportunities for social partners across both situations to exercise agency and engagement to improve PwD work integration.Originality/valueBy integrating two streams of literature – social policy and welfare state and industrial relations – this paper examines PwD work integration from a social ecosystem perspective. Empirically, it offers novel qualitative comparative evidence on trade unions’ and employers’ roles in Slovakia and Norway.

Adverse childhood experiences and yoga as "a practice of liberation".

The long-term negative health consequences of adverse childhood experiences (ACEs) pose a significant public health concern. Effective and engaging interventions that simultaneously address physical, mental, and socio-emotional health and mitigate the negative sequelae related to ACEs are needed. Promising evidence points to the impact of yoga and mindfulness on physiological and emotional health outcomes. Yet, little is known about those who have experienced multiple ACEs and their perspectives on the influence of yoga and mindfulness on their lives. The current study examines the phenomenological experiences of adults with high ACEs who engage in yoga practice. Twelve adults participated in in-depth, semistructured interviews. These included nine cisgender women, two cisgender men, and one gender nonbinary individual; 66.8% White, 16.6% multiracial, 8.3% Black/African American, and 8.3% Asian American/Pacific Islander. Participants were aged 20-63 years and endorsed four or more ACEs. They practiced yoga at least once a week for at least 6 months. Themes that emerged included: healing from trauma-related symptoms, integration of the whole self in mind-body practice, corrective experiences through yoga and mindfulness, and healing beyond talk therapy. Individuals with ACEs may experience yoga as a potentially valuable method for promoting healing through an integrative approach. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Short-Term Structural Response of Integral Abutment Bridge Approach Slabs Subjected to Live Loading and Thermal Effects

Short-Term Structural Response of Integral Abutment Bridge Approach Slabs Subjected to Live Loading and Thermal Effects

How can gender considerations be better integrated into animal health emergency preparedness and response?

Global and national authorities have not historically approached animal health emergencies through a gendered lens. Yet these events almost certainly have gendered dimensions, such as differential engagement of women or men depending on their culturally accepted or assigned roles for animal care; risk of exposure to zoonoses; and access to emergency resources during response and recovery. Despite the role that gender seems to play with respect to animal health emergencies, little research has been conducted to better understand such dynamics, and little policy has been promulgated to address it in a way that optimizes response while ensuring equitable outcomes. This piece summarizes 3 key themes that emerged from a panel discussion on gender and animal health emergencies at the World Organisation for Animal Health Global Conference on Emergency Management in April 2023. These themes were differential gendered exposure to pathogens; a lack of equitable gender representation in animal health decision-making; and enhancement of pathways for recognizing gender in national and international actions in preparing for, detecting, and responding to animal health emergencies. Beyond increasing opportunities for women to engage in leadership, the animal health and veterinary communities will benefit from connecting practitioners with gender experts to develop more integrative approaches to emergency preparedness and management. Animal health professionals should also advocate for further research to elucidate gender-specific dynamics in human populations in the context of animal emergencies and the promulgation of evidence-based policies. Such transformative efforts will lead to better outcomes for all people who depend on and provide care for animals.

SKIPping With PAX: Evaluating the Effects of a Dual-Component Intervention on Gross Motor Skill and Social–Emotional Development

Improving the development of the social, emotional, and physical domains during early childhood impacts the overall trajectory of a child’s well-being. However, researchers often address these independently, leaving a gap for a more integrated approach to promoting development. This study explores the effects of a dual-component intervention on changes in preschool-aged boys’ and girls’ gross motor and social–emotional skills. Preschoolers (N = 475; girls = 220 and boys = 255) ages 3–6 years completed the 9-month dual-component intervention and were randomized into control (n = 148) or intervention (n = 327) groups by classroom. Significant improvements were observed in social skills, locomotor, and total Test of Gross Motor Development-3. Additionally, boys and girls improved at the same rate in ball skills, locomotor, and total Test of Gross Motor Development-3. These results suggest that the dual-component intervention can improve preschoolers’ social skills and motor skills with no differential effects.

An Offline–Online Integration Approach for Security Traffic Patrolling With Frequency Constraints

An Offline–Online Integration Approach for Security Traffic Patrolling With Frequency Constraints

Damage Detection in Structural Health Monitoring Using an Integrated ANNIRSA Approach

In the past decade, artificial neural networks (ANNs) have been widely employed to address many problems. Despite their powerful problem-solving capabilities, ANNs are susceptible to a significant risk of stagnation in local minima due to using backpropagation algorithms based on gradient descent (GD) for optimal solution searching. In this paper, we introduce an enhanced version of the reptile search algorithm (IRSA), which operates in conjunction with an ANN to mitigate these limitations. By substituting GD with IRSA within an ANN, the network gains the ability to escape local minima, leading to improved prediction outcomes. To demonstrate the efficacy of IRSA in enhancing ANN’s performance, a numerical model of the Nam O Bridge is utilized. This model is updated to closely reflect actual structural conditions. Consequently, damage scenarios for single-element and multielement damage within the bridge structure are developed. The results confirm that ANNIRSA offers greater accuracy than traditional ANNs and ANNRSAs in predicting structural damage.

Enhancing pakchoi (Brassica chinensis L.) agriculture with magnetized-ionized brackish water and organic fertilizers: A sustainable approach to soil quality and crop yield optimization

The scarcity of freshwater, the increasing use of brackish water (B) for irrigation, and the judicious application of organic fertilizers have significant research and practical value for environmental science, water resource management, and clean production. This study investigated the combined impact of B, magnetized-ionized brackish water (MIB), and organic fertilizer on pakchoi (Brassica chinensis L.) growth, yield, and soil quality. Over a two-year period (2022–2023), this study explored the impacts of two types of irrigation water (B and magnetized-ionized brackish water, MIB) in combination with varying rates of water-soluble bio-organic fertilizer (0, 20, 40, 60, and 80 kg/ha, corresponding to treatments 0 through 4, respectively). The findings indicated that the application of 60 kg/ha of organic fertilizer greatly improved the growth rate of pakchoi in terms of plant height, leaf area index, and fresh weight. Moreover, under MIB irrigation, the fresh weight growth rate of pakchoi was superior to that observed under B irrigation. Notably, B irrigation necessitated additional fertilizer to maintain elevated levels of alkali-hydrolyzed nitrogen (AN) and soil quality. Furthermore, the use of organic fertilizer substantially enhanced soil quality, with AN, available phosphorus (AP), organic matter (OM), and soil quality index (SQI) demonstrating a strong positive correlation (P < 0.05). Mantel test analysis showed significant correlations (P < 0.05) between soil OM, SWC, AP, and pakchoi growth and yield. According to the partial least squares path modeling analysis, the use of organic fertilizer had a relatively minor effect on the yield and quality of pakchoi under B irrigation, with path coefficients of 0.088 and 0.298, respectively. However, under MIB irrigation, organic fertilizer enhances yield and quality by improving soil quality (path coefficients of 0.914). In conclusion, the application of MIB irrigation coupled with organic fertilizer contributed to sustainable soil fertility, enhanced crop productivity, and mitigated the environmental impacts associated with conventional irrigation and fertilization methods. Our research underscores the importance of supporting agricultural research and development focused on sustainable irrigation and fertilization methods for farmers and policymakers. This integrated approach offers a harmonious solution for optimizing crop yields and enhancing water use efficiency, and the reduction of in chemical pollutants within agricultural production systems.

Integrated Approach to Currency Factor Investing

Using the G10 universe of currencies, we evidence that parametric portfolio policies can help guide an optimal currency strategy when tilting towards cross-sectional factor characteristics. While currency carry serves as the main return generator in this tilting strategy, the two characteristics momentum and value are implicit diversifiers to potentially balance the downside of carry investing in flight-to-quality shifts of FX investors. Drawing insights from a currency timing strategy according to time series predictors, we further examine the parametric portfolio policy's ability to mitigate the downside of the carry trade by incorporating an explicit currency factor timing element. This integrated approach to currency factor investing outperforms a naive equally weighted benchmark as well as univariate and multivariate parametric portfolio policies.

Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum

Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host–microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated under heat stress conditions. In contrast, pathways related to the cell cycle were suppressed under normal environmental temperatures. In line with the transcriptome analysis, the microbial analysis results indicate that taxonomic changes affect lipid degradation. Heat stress engendered statistically significant difference in the abundance of 11 microorganisms, including Bacteroides and Peptostreptococcacea. Together, integrative approach analysis suggests that microbiota-induced metabolites affect host fatty acid peroxidation metabolism, which is correlated with the gene families of Acyl-CoA dehydrogenase long chain (ACADL), Acyl-CoA Oxidase (ACOX) and Acetyl-CoA Acyltransferase (ACAA). This integrated approach provides novel insights into heat stress problems and identifies potential biomarkers associated with heat stress.

Abstract 2961: Investigating the role of aging and treatment-induced senescence in the metastatic liver cancer tumor microenvironment

Abstract Liver senescence, marked by the progressive decline in hepatic cell function and regenerative capacity, plays a vital role in the context of aging, chronic liver diseases, and cancer progression, particularly when influenced by anti-cancer therapies. This study is designed to extensively characterize liver senescence under two specific conditions: replicative aging and therapy-induced changes. We examined liver samples from 20 individuals, covering a wide age range (young, &amp;lt;40 years; old, &amp;gt;60 years), and 16 cases of colorectal cancer (CRC) liver metastasis (ages 37-66). Our integrative approach melds single-nucleus RNA-sequencing (snRNA-seq) and ATAC-sequencing (snATAC-seq) to investigate the transcriptomic and epigenetic landscapes across a spectrum of liver cell types, including hepatocytes, cholangiocytes, stellate cells, liver sinusoidal endothelial cells, and diverse immune cells. Key findings include an upregulation of senescence markers such as CDKN1A, CDKN2A, and HMGB1, particularly in specific hepatocytes from older individuals and various cell types in the CRC liver metastasis group. Furthermore, we noted a distinctive senescence-associated secretory phenotype (SASP) across multiple immune cell types in our treated CRC liver metastasis group and upregulation of treatment-induced senescence gene signatures, such as SenCan. Moreover, hepatic stellate cells from older subjects exhibited augmented senescence-related gene signatures (SenSig) and transcription factors (SenMayo), with myofibroblast-like and FAP+ stellate cells showing a marked senescence phenotype, evidenced by elevated SASP molecule expression (CCL2, SERPINE1, GLB1, IGFBP7, IGFBP3).Incorporating advanced high-resolution imaging techniques, our study delves into the detailed morphological characteristics and spatial distribution of senescence markers within the liver in the setting of replicative aging and treatment-induced senescence. This study sheds light on the heterogeneity and intricacies of senescence-associated gene expression within liver cell populations. By combining comprehensive multiomic data with advanced high-resolution spatial profiling, we aim to decipher unique senescence phenotypes in the context of both replicative and treatment-induced senescence. Our work highlights the significance of cellular senescence in liver pathologies and cancer progression, suggesting new directions for therapeutic interventions that target these complex dynamics. Citation Format: Kelsey Gallant, Alla Karpova, Xiang Li, Michael Iglesia, Andrew Houston, Daniel Rapp, Chien-Wei Peng, Clara Liu, John Herdon, Feng Chen, Ryan Fields, Li Ding. Investigating the role of aging and treatment-induced senescence in the metastatic liver cancer tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2961.

Abstract 6219: Type V collagen promotes lung adenocarcinoma metastasis by regulating tumor mechanical stress and the implication for prognosis and therapeutic strategies

Abstract Introduction: The mechanical environment of the human body influences physiological and pathological processes, and understanding these biomechanical aspects holds promise for medical research. Tumor microenvironment changes, including extracellular matrix (ECM) alterations, result the changing of mechanical characteristics of the tissue, play a crucial role in cancer progression and metastasis, including lung adenocarcinoma (LUAD), one of a major histology type of lung cancer. This study focuses on elucidating how mechanical characteristics, especially those related to the ECM, influence LUAD and the signaling pathways involved. Methods: Cell culture, transfection, Western blot, immunofluorescence, immunohistochemistry, Transwell assay, scratch wound-healing assay were used to investigate the behavior changes of LUAD cells under different treatment. Bioinformatics analyses, and machine learning-based integrative approaches were employed to build the SVM_Score, which was built with relevant base membrane (BM) genes. Patient-derived tumor organoids, myofibroblasts, and co-culture systems were established. Sorafenib, which can inhibited collagen and fibronectin synthesis, was used to explore the proliferation and migration changing of lung cancer cells co-cultured with myofibroblasts. Results: Mechanical stress, simulated by matrix application, enhanced LUAD cell migration and invasion, correlating with ECM alterations and EMT pathway activation. SVM_Score predicted LUAD patient prognosis and EMT propensity across multiple datasets, revealing its robust prognostic capabilities. Lower SVM_Scores were associated with worse survival outcomes, increased cancer-related pathways, higher Tumor Mutation Burden and altered immune microenvironment characteristics. SVM_Score was also linked to myofibroblast-secreted COL5A1, a key marker for mechanical stress. Patient tissues with low SVM_Scores exhibited higher COL5A1 expression, enhanced EMT propensity, and increased internal mechanical stress. Sorafenib, by inhibiting COL5A1 secretion, attenuated the pro-tumor effects of myofibroblasts, inhibiting proliferation and migration of LUAD cells, and rendering LUAD cells more sensitive to chemotherapy. Conclusion: This comprehensive study unveils the intricate relationship between mechanical stress, ECM alterations, and LUAD progression. SVM_Score emerges as a potent prognostic tool, reflects tumor mechanical characteristics. Sorafenib intervention targeting COL5A1 secretion provides a potential therapeutic strategy to mitigate LUAD aggressiveness. These findings contribute to a deeper understanding of the biomechanical aspects of LUAD, offering insights for future research and clinical applications. Citation Format: Guangsheng Zhu, Yanan Wang, Yingjie Wang, Chen, Yongwen Li, Hongyu Liu, Jun Chen. Type V collagen promotes lung adenocarcinoma metastasis by regulating tumor mechanical stress and the implication for prognosis and therapeutic strategies [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6219.

Unravelling the genetic diversity and taxonomic ambiguities of endemic Petunia species from subtropical highland grasslands

Abstract Closely related and young species often show high morphological similarity, challenging their identification and correct assignment. Molecular markers and integrative approaches have contributed to solving many taxonomic uncertainties. In this study, we evaluated the genetic variability and ecological features of Petunia guarapuavensis and Petunia scheideana, over which there is a taxonomic debate. Both species are endemic and rare, distributed in the subtropical highland grasslands in southern South America. We based our analyses on nuclear microsatellite and plastid sequences, aiming to disentangle the taxonomic ambiguities that made some consider these entities synonymous despite occupying different clades in the genus phylogenetic tree. Our findings support that there is genetic differentiation between these species, suggesting that they are independent taxonomic entities despite sharing floral traits and a few molecular polymorphisms. The low genetic sharing between the species is likely due to a common ancestor and recent divergence time. In contrast, their morphological similarity can be attributed to the absence of selective pressure, as both grow under similar ecological conditions. This study emphasizes that adding more than one sequence per species, combining data with dissimilar inheritance patterns, and exploring data through different methodologies help to disentangle taxonomic incongruences and reveal diversity that might otherwise remain hidden.

Aging Increases Global Annual Food Greenhouse Gas Emissions up to 300 Million Tonnes by 2100.

The dietary preferences of the elderly population exhibit distinct variations from the overall averages in most countries, gaining increasing significance due to aging demographics worldwide. These dietary preferences play a crucial role in shaping global food systems, which will result in changed environmental impacts in the future such as greenhouse gas (GHG) emissions. We present a quantitative evaluation of the influence of population aging on the changes in GHG emissions from global food systems. To achieve this, we developed regional dietary coefficients (DCs) of the elderly based on the Global Dietary Database (GDD). We then reconciled the GDD with the dataset from the Food and Agriculture Organization of the United Nations (FAO) to calculate the food GHG emissions of the average population in each of the countries. By applying the DCs, we estimated the national food GHG emissions and obtained the variations between the emissions from aged and average populations. We employed a modified version of the regional integrated model of climate and the economy model (RICE) to forecast the emission trends in different countries based on FAO and GDD data. This integrated approach allowed us to evaluate the dynamic relationships among aging demographics, food consumption patterns, and economic developments within regions. Our results indicate that the annual aging-embodied global food GHG emissions will reach 288 million tonnes of CO2 equivalent (Mt CO2e) by 2100. This estimation is crucial for policymakers, entrepreneurs, and researchers as it provides insights into a potential future environmental challenge and emphasizes the importance of sustainable food production and consumption strategies to GHG emission mitigations associated with aging dietary patterns.

Formulation and Validation of Organic Insect Pest Management Module for Pests of Cabbage and Cauliflower in Valley and Foot Hills Region, Manipur, India

During the rabi seasons from 2017 to 2020 in the valley and foothill regions of Imphal, Manipur, a study was conducted to design and test three different organic insect pest management modules M1, M2 and M3 for cabbage and cauliflower. The focus was on three major pests: cabbage butterfly, diamondback moth, and cabbage aphid. Module three (M-3) emerged as the most effective strategy, incorporating various organic practices. This included seed treatment with Trichoderma harzianum at 10g/kg seed and soil drenching in nursery plots at 25g/100 m2, intercropping with mustard, application of anonnin extract at 5 ml/litre, Spinosad 45% SC (biopesticide) at 3ml/10 litres, and Verticillium lecanii at 10 ml/litre. Additionally, the module incorporated the installation of yellow sticky traps for aphids and pheromone traps for diamondback moths for monitoring and mass trapping. The results indicated that Module 3 performed well than other modules and the control group in reducing insect pest incidence on cabbage and cauliflower with the number of cabbage butterfly ranging from 0.07 to 0.11, in both cabbage and cauliflower, where as 0.06 to 0.04 in case of Diamond back moth and 0.21 to 0.47 number of cabbage aphid per square inch leaf area with B:C ratio of 1:12.4 and 1:12.7 for cabbage and 1:6:39 and1:6.41 for cauliflower at lamphel and lanol fields, respectively. Moreover, it contributed to an increased yield, demonstrating its superiority in organic insect pest management for both valley and foothill regions. This underscores the effectiveness of a holistic and integrated approach to pest management in organic farming practices.

Unraveling the role of low-density lipoprotein-related genes in lung adenocarcinoma: Insights into tumor microenvironment and clinical prognosis.

The hypothesized link between low-density lipoprotein (LDL) and oncogenesis has garnered significant interest, yet its explicit impact on lung adenocarcinoma (LUAD) remains to be elucidated. This investigation aims to demystify the function of LDL-related genes (LRGs) within LUAD, endeavoring to shed light on the complex interplay between LDL and carcinogenesis. Leveraging single-cell transcriptomics, we examined the role of LRGs within the tumor microenvironment (TME). The expression patterns of LRGs across diverse cellular phenotypes were delineated using an array of computational methodologies, including AUCell, UCell, singscore, ssGSEA, and AddModuleScore. CellChat facilitated the exploration of distinct cellular interactions within LDL_low and LDL_high groups. The findmarker utility, coupled with Pearson correlation analysis, facilitated the identification of pivotal genes correlated with LDL indices. An integrative approach to transcriptomic data analysis was adopted, utilizing a machine learning framework to devise an LDL-associated signature (LAS). This enabled the delineation of genomic disparities, pathway enrichments, immune cell dynamics, and pharmacological sensitivities between LAS stratifications. Enhanced cellular crosstalk was observed in the LDL_high group, with the CoxBoost+Ridge algorithm achieving the apex c-index for LAS formulation. Benchmarking against 144 extant LUAD models underscored the superior prognostic acuity of LAS. Elevated LAS indices were synonymous with adverse outcomes, diminished immune surveillance, and an upsurge in pathways conducive to neoplastic proliferation. Notably, a pronounced susceptibility to paclitaxel and gemcitabine was discerned within the high-LAS cohort, delineating prospective therapeutic corridors. This study elucidates the significance of LRGs within the TME and introduces an LAS for prognostication in LUAD patients. Our findings accentuate putative therapeutic targets and elucidate the clinical ramifications of LAS deployment.

Medical professional liability insuarance

The aim of the study is to form an integrated approach to understanding the context and legal necessity of medical professional liability insurance.&#x0D; The methodological basis of the study is a set of scientific techniques and methods of scientific knowledge, including dialectical, historical-legal, logical-legal, system-structural, method of forecasting and comparative jurisprudence. Dialectical method has provided opportunities to analyze scientific positions regarding the content and development of medical professional liability insurance. Historical-legal method helps to study the genesis of the development of insurance in specific historical and legal situations, the development of theoretical scientific thought and legislation. The method of comparative law is used to study the legislative and regulatory acts of Ukraine and other countries in the world as well as to clarify the advantages and disadvantages of the mechanism of legal regulation the insurance of medical professional liability in various countries in the world. Systemic and structural methods have helped to clarify the content of the contracts connected with this kind of insurance. Formal-logical method has allowed analyzing the provisions of the legislation of Ukraine in the sphere of this type of insurance.&#x0D; Results. This study contains a comprehensive scientific analysis of modern problems of medical professional liability insurance. The elements of the medical professional liability insurance contract are defined, namely: subject matter, insured, beneficiary, insurance risk, insurance event, conditionals of insurance compensation payment. It is stipulated that medical professional liability insurance contract shall have separate grounds for refusal to pay insurance compensation, inherent only to this type of insurance. It is shown that this type of insurance helps to manage the risks in medical practice activities. The concept of this type of insurance has been researched. The conditions to terminate the medical professional liability insurance have been identified.&#x0D; Conclusions. Having considered the issues of medical professional liability insurance and examined the conditions of the medical professional liability insurance contract, it has been made a conclusion that it is necessary to introduce this type of insurance as mandatory for healthcare institutions and private practitioners. It has been proposed to introduce compulsory medical professional liability insurance by approving necessary alterations the valid legislation.

Toward an integrative approach to translational exercise biomedicine

Toward an integrative approach to translational exercise biomedicine