Insufficient Energy Supply Research Articles

Energy Needs and Trends Assessment for the Urban Commune of N’zérékoré, Republic of Guinea

Nowadays, one of the major problems facing large cities is the adaptation of energy needs with their rapid demographic growth. In most cases, there is a disconnect between the rapid demographic increases and the updating of energy needs. The major cities of the Republic of Guinea are confronted to a significant challenge due to insufficient energy supply for their sustainable development, and the urban commune of N’zérékoré is a clear example of this. The city is experiencing substantial rapid demographic growth, which requires a long-term assessment of its energy needs for its sustainable development. In this paper, a model for projecting energy demand over 10 years for the urban commune of N'zérékoré taking into account its demographic evolution was developed. An estimate of energy needs was made based on the projected population growth from 2024 to 2060. The results show that the urban commune's current energy consumption is estimated at 53.89 MW, or 38.8 GWh per month, while the projected energy demand for the same year amounts to 51.6 GWh. Population growth continuously drives a strong increase in energy demand, if production remains unchanged, by 2060 the commune’s energy need would reach 1,595.16 GWh, resulting in a deficit of 1,543.56 GWh.

Carbon-Oxygen Radical Assisted Growth of Defect-Free Graphene Films Using Low-Temperature Chemical Vapor Deposition.

Low-temperature chemical vapor deposition growth of graphene films is a long-term pursuit in the graphene synthesis field because of the low energy consumption, short heating-cooling process and low wrinkle density of as-obtained films. However, insufficient energy supply at low temperature (below 850°C) usually leads to the difficulty in carbon source dissociation, graphene growth, and defect healing. Herein, a Carbon-Oxygen (C─O) radical assisted strategy is proposed for low-temperature growth of defect-free, wrinkle-free, and single-crystalline graphene films by using methanol precursor. We provide a deep insight into the growth process fueled by methanol precursor, unveiling the dissociation pathway of methanol and the roles of intermediate C─O radicals in carbon attaching and assembling to graphene lattice without defect formation. This method shows promising prospects in the cost-effective production of high-quality graphene films and provides inspiration for growing other 2D materials.

Superhydrophobic Coatings with Synergistically Enhanced Anti/Deicing Performance by Optically/Electrically Assisted Heating

Surface icing issues have a significant impact on industries such as aviation, transportation, and construction. Superhydrophobic surfaces can delay ice formation due to their liquid‐repellent properties, but their effectiveness is not pronounced in extremely cold environments. Electric heating coatings can effectively prevent ice formation, but they have limitations in environments with insufficient electrical energy supply. The anti‐icing effect of photothermal superhydrophobic coatings is restricted under conditions of insufficient sunlight. To enhance the ice‐preventing performance of superhydrophobic coatings in extremely cold environments, this article employs a template spraying method to prepare a carbon black and graphene composite coating that provides superhydrophobic passive anti‐icing and photo/electrothermal active deicing capabilities. The micro‐nanostructured superhydrophobic surface exhibits exceptional ice‐preventing performance. The excellent electrothermal and photothermal performance, along with high energy conversion efficiency, significantly enhance the coating's deicing efficiency. Under the synergistic effect of solar and electrical energy, the ice layer is completely melted within just 135 s. Furthermore, the material possesses excellent durability (resistance to mechanical wear, acid and alkali corrosion, and UV aging), as well as thermal stability. This research provides new avenues and insights for the development of advanced anti‐icing and deicing materials for applications in aviation, transportation, construction, and other fields.

Heat stress and bleaching in corals: a bioenergetic model

The coral-dinoflagellate endosymbiosis is based on nutrient exchanges that impact holobiont energetics. Of particular concern is the breakdown or dysbiosis of this partnership that is seen in response to elevated temperatures, where loss of symbionts through coral bleaching can lead to starvation and mortality. Here we extend a dynamic bioenergetic model of coral symbioses to explore the mechanisms by which temperature impacts various processes in the symbiosis and to enable simulational analysis of thermal bleaching. Our model tests the effects of two distinct mechanisms for how increased temperature impacts the symbiosis: 1) accelerated metabolic rates due to thermodynamics and 2) damage to the photosynthetic machinery of the symbiont caused by heat stress. Model simulations show that the model can capture key biological responses to different levels of increased temperatures. Moderately increased temperatures increase metabolic rates and slightly decrease photosynthesis. The slightly decreased photosynthesis rates cause the host to receive less carbon and share more nitrogen with the symbiont. This results in temporarily increased symbiont growth and a higher symbiont/host ratio. In contrast, higher temperatures cause a breakdown of the symbiosis due to escalating feedback that involves further reduction in photosynthesis and insufficient energy supply for CO2\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\hbox {CO}_2$$\\end{document} concentration by the host. This leads to the accumulation of excess light energy and the generation of reactive oxygen species, eventually triggering symbiont expulsion and coral bleaching. Importantly, bleaching does not result from accelerated metabolic rates alone; it only occurs as a result of the photodamage mechanism due to its effect on nutrient cycling. Both higher light intensities and higher levels of DIN render corals more susceptible to heat stress. Conversely, heterotrophic feeding can increase the maximal temperature that can be tolerated by the coral. Collectively these results show that a bioenergetics model can capture many observed patterns of heat stress in corals, such as higher metabolic rates and higher symbiont/host ratios at moderately increased temperatures and symbiont expulsion at strongly increased temperatures.

Acetylshikonin reduces the spread of antibiotic resistance via plasmid conjugation

The plasmid-mediated conjugative transfer of antibiotic resistance genes (ARGs) stands out as the primary driver behind the dissemination of antimicrobial resistance (AMR). Developing effective inhibitors that target conjugative transfer represents an efficient strategy for addressing the issue of AMR. Here, we studied the effect of acetylshikonin (ASK), a botanical derivative, on plasmid conjugation. The conjugative transfer of RP4-7 plasmid inter and intra species was notably reduced by ASK. The conjugation process of IncI2 and IncX4 plasmids harboring the mobile colistin resistance gene (mcr-1), IncX4 and IncX3 plasmids containing the carbapenem resistance gene (blaNDM-5), and IncFI and IncFII plasmids possessing the tetracycline resistance gene [tet(X4)] were also reduced by ASK. Importantly, the conjugative transfer frequency of mcr-1 positive IncI2 plasmid in mouse peritoneal conjugation model and gut conjugation model was reduced by ASK. The mechanism investigation showed that ASK disrupt the functionality of the bacterial cell membrane. Furthermore, the proton motive force (PMF) was dissipated. In addition, ASK blocked the electron transmission in bacteria's electron transport chain (ETC) through disturbing the quinone interaction, resulting in an insufficient energy supply for conjugation. Collectively, ASK is a potential conjugative transfer inhibitor, providing novel strategies to prevent the spread of AMR.

Conversion of lipids into carbohydrates rescues energy insufficiency in rapeseed germination under waterlogging stress.

Waterlogging stress, particularly during seed germination, significantly affects plant growth and development. However, the physiological and molecular mechanisms underlying waterlogging stress responses during rapeseed germination remain unclear. In this study, two rapeseed cultivars, Xiangzayou518 (waterlogging-sensitive) and Dadi199 (waterlogging-tolerant), were used to explore the physiological mechanisms underlying rapeseed response to waterlogging stress during germination. Our results showed that waterlogging significantly decreased the emergence percentage and seedling growth rate. During the radicle elongation period (from 48 to 96 h post-germination), the most sensitive period to waterlogging during germination, sugar content, and glycolysis efficiency were significantly decreased, but anaerobic fermentation was enhanced. In tolerant cultivars, when the energy supply was insufficient, the conversion efficiency of lipids into sugar increased, and the activities of isocitrate lyase, malate synthase, and fructose-1, 6-diphosphatase were enhanced by 11.63, 19.06, and 20.37%, respectively, at 72 h post-germination under waterlogging stress. Transcriptome data showed that the differentially expressed genes were significantly enriched in glucose and lipid metabolism pathways when comparing waterlogged stress and normal conditions. These results indicate that waterlogging affects seed germination in rapeseed by inhibiting carbohydrate metabolism, and the conversion capacity of lipids into sugar under waterlogging stress was stronger in tolerant cultivars than in sensitive cultivars, thus rescuing the insufficient energy supply in seed germination and seedling growth. This study reveals the physiological mechanism of rapeseed response to waterlogging stress during seed germination and provides a valuable reference for improving waterlogging tolerance.

Repeated marine heatwaves aggravate the adverse effects of nano-TiO2 on physiological metabolism of the thick-shelled mussel Mytilus coruscus

Global climate change is a major trigger of unexpected temperature fluctuations. The impacts of marine heatwaves (MHWs) and nano-titanium dioxide (nano-TiO2) on marine organisms have been extensively investigated. However, the potential mechanisms underlying their interactive effects on physiological processes and metabolism remain poorly understood, especially regarding periodic MHWs in real-world conditions. In this study, the effects of nano-TiO2 (at concentrations of 0, 25, and 250 μg/L) and periodic MHWs on the condition index (CI) and underlying metabolic mechanisms were investigated in mussels (Mytilus coruscus). The results showed that mussels try to upregulate their respiration rate (RR) to enhance aerobic metabolism (indicated by elevated succinate dehydrogenase) under short-term nano-TiO2 exposure. However, even at ambient concentration (25 μg/L), prolonged nano-TiO2 exposure inhibited ingestion ability (decreased clearance rate) and glycolysis (inhibited pyruvate kinase, hexokinase, and phosphofructokinase activities), which led to an insufficient energy supply (decreased triglyceride, albumin, and ATP contents). Repeated thermal scenarios caused more severe physiological damage, demonstrating that mussels are fragile to periodic MHWs. MHWs decreased the zeta potential of the nano-TiO2 particles but increased the hydrodynamic diameter. Additionally, exposure to nano-TiO2 and periodic MHWs further affected aerobic respiration (inhibited lactate dehydrogenase and succinate dehydrogenase activities), metabolism (decreased RR, activities of respiratory metabolism-related enzymes, and expressions of PEPCK, PPARγ, and ACO), and overall health condition (decreased ATP and CI). These findings indicate that the combined stress of these two stressors exerts more detrimental impact on the physiological performance and energy metabolism of mussels, and periodic MHWs exacerbate the toxicological effects of ambient concentration nano-TiO2. Given the potential worsening of nanoparticle pollution and the increase in extreme heat events in the future, the well-being of mussels in the marine environment may face further threats.

Experimental analysis on improving heat storage efficiency of high-temperature packed bed system using spherical capsules filled with MgCl2–KCl–NaCl/Al2O3 nanoparticles composite phase change material

While thermal energy storage (TES) systems are becoming crucial to the stable operation of solar power plants, molten salt packed bed thermal energy storage (PBTES) systems attracted widespread attention due to their wide operating temperature range and low cost. However, achieving high-efficiency thermal storage systems is still a datum challenge to meet the higher energy demand, as well as sustainable development and utilization of renewable energy systems. This study developed spherical capsules employing nanocomposite salts (MgCl2–KCl–NaCl/Al2O3 nanoparticles) as composite phase change materials to experimentally study the heat storage process of a high-temperature PBTES prototype experimental system. Also, the heat storage performance of the PBTES system under different operating conditions is further investigated. The results showed that the nanocomposite salt gradually melted along the flow direction of the heat transfer fluid by exhibiting three-stage characteristics including solid phase sensible heat, phase change latent heat, and liquid phase sensible heat. Also, the temperature difference inside and outside the capsules showed the characteristics of peak-trough-peak. The PBTES system reached a heat storage density of 1290.51 MJ m−3 at a thermal efficiency of 86.95 %. The average heat storage rate increased by 49.55 % and the heat storage time was shortened by 29.67 % when the blower speed was increased from 800 r·min−1 to 1200 r·min−1. The experimental results implied that the PBTES system encapsulating nanocomposite salts has excellent thermal performance. This study demonstrated solving the problems of insufficient thermal energy supply in time or space and improving energy utilization could result in a high-efficiency thermal storage system.

End-to-end learning for stochastic preventive dispatch of renewables-rich power systems in abnormal weather conditions

Power systems are currently increasingly threatened by abnormal weather conditions, which are exacerbated by global warming. In particular, the proliferation of renewable energy sources (RES), which are quite variable, might cause power systems to have insufficient energy supply to cope with the risk of severe power imbalances in such conditions. In this paper, a preventive power system dispatch model is proposed that allows power systems with large RES (specifically wind energy) installations to mitigate excessive load curtailments under abnormal weather conditions. Compared to the conventional predict-then-optimize (PTO) framework of power system dispatch that relies on accurate RES forecasting, the proposed end-to-end learning framework mitigates the impact of forecasting errors on decision-making, which reduces the dependence on the accuracy of RES forecasting and effectively enhances the resilience of the power system. The proposed framework consists of a probabilistic RES forecasting module, a preventive dispatch module, and a resilience-oriented assessment module. A backpropagation mechanism based on Karush-Kuhn-Tucker conditions is proposed to unify the dispatch optimization problem as deep neural networks, which ensures the reliability of optimal solutions as well as provides necessary gradients for backpropagation, successfully bridging the information gap among the three modules. The proposed model is updated iteratively in an online implementation scheme composed of the decision-making part and the reflection & training part. The results of numerical experiments on the IEEE 6- and 118-bus systems verify the effectiveness and superiority of the proposed model in maintaining the resilience of RES-based power systems.

Integrative multi-omics analysis reveals the underlying toxicological mechanisms of enrofloxacin on the growth of wheat seedling roots

The continuous release of antibiotics into agroecosystems has raised concerns about the potential negative effects of antibiotic residues on crops. In this study, the toxicological effects of enrofloxacin (ENR) on wheat seedlings were analyzed using a combination of morpho-physiological, transcriptomic, proteomic, and metabolomic approaches. ENR inhibited the growth of wheat (Triticum aestivum L.) roots and induced oxidative stress. In particular, ENR downregulated the oxidative phosphorylation pathway, while it enhanced glycolysis and the tricarboxylic acid cycle, thereby regulating the balance of intracellular energy metabolism. In addition, sustained exposure to excessive reactive oxygen species (ROS) resulted in an increase in reduced glutathione (GSH), a slight decrease in ascorbic acid (AsA), and a significant decrease in the ratio of GSH to oxidized glutathione (GSSG), which imbalanced the AsA-GSH cycle. In addition, the resulting increase in abnormal proteins triggered ubiquitin-independent proteasomal degradation pathways. Further, an increase in abscisic acid (ABA) and a decrease in jasmonic acid (JA) and its derivatives alleviated the inhibitory effect of ENR on the growth of wheat roots. In conclusion, direct damage and signaling by ROS, hormonal regulation, a decrease in the GSH to GSSG ratio, and insufficient energy supply were identified as key factors for the significant inhibition of wheat root growth under ENR stress.

Transcriptome-Based Analysis of the Mechanism of Action of Metabolic Disorders Induced by Waterborne Copper Stress in Coilia nasus.

To reveal the effects of waterborne copper stress on gene expression changes, molecular pathways, and physiological functions in Coilia nasus, juvenile fish were equally divided into two experimental groups, and the copper levels were 1.61 ± 0.03 mg/L (copper-exposed group) and 0 mg/L (control group), respectively. After 4 h, gill tissue samples were collected for transcript sequencing analysis, and two libraries were constructed from the copper treatment group (Cu) and the control group (C) and sequenced using Illumina sequencing technology. The results showed that approximately 40.2-46.0 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.57 to 84.93%. A total of 3915 differentially expressed genes (DEGs) were identified under waterborne copper stress, among which 1300 genes were up-regulated, and 2615 genes were down-regulated. Twelve DEGs were randomly selected for quantitative RT-PCR (qRT-PCR) analysis, and the results confirmed that the transcriptome analysis was reliable. Furthermore, the DEGs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and the results showed that most of the DEGs were involved in metabolic pathways, including steroid biosynthesis, glutathione metabolism, and peroxisome proliferator-activated receptor (PPAR) signaling pathways. Furthermore, due to the waterborne copper levels, gsk-3β was significantly up-regulated, while other metabolism-related genes (tor, pi3k, lpl, aqp7, fabp3) were significantly down-regulated. In addition, the copper-exposed group significantly reduced the expression of some immunity genes (ifn-γ, stat1, cxcl10, and tgf-β), and enhanced the expression of il-1β and tnf-α. In summary, these results indicated that copper causes metabolic disorders and insufficient energy supply in the body, and induces oxidative stress, which results in reduced immune functions.

The role of miRNA-29b1 on the hypoxia-induced apoptosis in mammalian cardiomyocytes.

Cardiomyocyte apoptosis is a complex biological process involving the interaction of many factors and signaling pathways. In hypoxic environment, cardiomyocytes may trigger apoptosis due to insufficient energy supply, increased production of oxygen free radicals, and disturbance of intracellular calcium ion balance. The present research aimed to investigate the role of microRNA-29b1 (miR-29b1) in hypoxia-treated cardiomyocytes and its potential mechanism involved. We established an in vitro ischemia model using AC16 and H9C2 cardiomyocytes through hypoxia treatment (1% O2, 48 h). Cell apoptosis was evaluated by flow cytometry using Annexin V FITC-PI staining assay. Moreover, we used Western blot and immunofluorescence analysis to determine the expression of Bcl-2, Bax caspase-3 and Cx43 proteins. We found that miR-29b1 protected AC16 and H9C2 cells from hypoxia-induced injury as evidence that miR-29b1 attenuated the effects of hypoxia treatment on AC16 and H9C2 cell apoptosis after hypoxia treatment. In conclusion, our findings suggest that miR-29b1 may have potential cardiovascular protective effects during ischemia-related myocardial injury.

Investigating the addition of Fe for improving contaminant removal and regulating microbes in a simulated coastal wetland.

Contaminants from wastewater of aquaculture are increasing the risks of red tides in coastal areas. Such types of contaminants are difficult to remove by using conventional biological and ecological treatment methods because of the relatively low C/N ratios and the high salinity in coastal water ambience. Fe is considered a key element in natural chemical cycling and promotes the growth of animals and plants as well. The cycling of Fe ion combined with carbon, nitrogen, and phosphorus stimulates bacterial growth. As a result, it acts as a microbial carbon pump in coastal areas, such as natural wetlands, which have been activated and adapted to be salinity resistant and insufficient energy supply. Along these lines, in this work, constructed wetlands (CWs) with high ecological benefits and low cost of maintenance were used to treat aquaculture wastewater. The impact of Fe ion recycling on multiple contaminants was also systematically investigated. The two types of Fe dosage were pure ferrous ions and a mixture of iron powder and ferrous ions. After the application of a 3-day treatment, the dosage of iron powder/ferric ions (1:1 m/m) at a concentration of 15 mg L-1 showed a better effect, where the total nitrogen, total phosphorus, and chemical oxygen demand removal rates were increased by 2.95%, 2.16%, and 9.76%, respectively. From the microbial analysis, it was indicated that Fe ion affected the abundance and functions of the microbial communities in the CWs. The significant enrichment of Proteobacteria promoted the removal of multiple contaminants under saline stress and fixed carbon, and affected the whole microbe distribution and diversity in CWs. The implementation of such an environmentally friendly and economical approach arises as a promising candidate for the efficient removal of multiple contaminants from aquacultural wastewater in coastal zones.

Contribution of Therapeutic Foods to the Nutritional Care of Malnourished Children in West Africa

Undernutrition is the most common form of malnutrition in West Africa, particularly in countries in the Sahel region. It constitutes a major public health problem in Africa and in the world. Caused by deficiency of one or more nutrients, undernutrition remains a major concern around the world and especially in the West African region where it contributes to a high number of deaths in children under five. It denotes an insufficient supply of energy and nutrients to meet an individual's needs for good health. Moreover, there are more and more centers specialized in nutritional care to help solve the problem of undernutrition in children. Thenceforward, knowledge of the current situation will allow better actions to overcome this form of malnutrition which grieves many families in our African regions and particularly in West Africa. This review reports on the nutritional care of malnourished children in West Africa. To do this, scientific databases such as PubMed, ScienceDirect, Scopus and Google Scholar were used to extract publications on the nutritional care of malnourished children. The data are analyzed to assess the management of cases of undernutrition in West Africa. These results can serve as a basis for better care of malnourished children in countries with limited resources, including those in West Africa.

Optimization of the performance and operation of a photovoltaic-thermoelectric power supply system for bridge safety monitoring microsystems

In the long-term unattended condition, the continuous operation of bridge safety monitoring micro-system (BSMMS) in mountain areas has the issue of insufficient energy supply. Therefore, a photovoltaic-thermoelectric hybrid power generation system (PV-TEG) is proposed to power the temperature, wind speed, and tilt sensors of BSMMS. The power generation performance and characteristics of PV and TEG are experimentally investigated to find the operation mode to match the bridge monitoring. In addition, the intermittent operation mode of BSMMS is optimized to achieve longer running times. The experimental results show that the output voltage and power generation of the PV-TEG can meet the energy consumption demands of all sensors. TEG power generation is linear with bridge temperature, which can trigger TEG to support the temperature sensor operation. The energy storage battery supports the BSMMS to run for 8.99 days in the mode of running 5 min every 5-min intervals. Optimizing the operation time and interval of the BSMMS to run once in half an hour, it can run continuously for up to 17.97–22.46 days. This work provides a reference for the design of an unattended self-powered energy supply system for a multi-parameter integrated bridge safety monitoring system.

Contemporary Resource-Based Energy Financial Trade

Currently, faced with the rapid increase in energy demand and insufficient energy supply, as well as the two major contradictions between energy consumption in production and living and comprehensive sustainable development, countries and regions have chosen to cooperate to jointly cope with development problems, and use the “Belt and Road” energy cooperation and global climate Energy cooperation represented by negotiations is not uncommon. The transformation of the resource-based economy has entered a critical period, and it is necessary to find strong theoretical support and feasible practical measures. Therefore, combining the proposition of energy cooperation with the transformation needs of the resource-based economy and studying the role of energy cooperation in the transformation of the resource-based economy from the perspective of factor combinations can not only lay a micro-foundation for issues related to energy cooperation at the meso-level in theory , opening up a new perspective for academic research related to resource-based economy; it can also guide the practice of resource-based economic transformation and explore breakthrough paths for it.

Dissolved oxygen and ammonia affect ammonia production via GDH/AMPK signaling pathway and alter flesh quality in Chinese perch (Siniperca chuatsi).

The dissolved oxygen (DO) and ammonia are crucial to the growth of Chinese perch (Siniperca chuatsi). Information on the effects of DO and total ammonia nitrogen (TAN) in regulating ammonia nitrogen excretion and flesh quality in Chinese perch is scanty. This study aimed to evaluate the effects of dissolved DO at oxygen levels of 3 mg/L and 9 mg/L, as well as the TAN concentrations of 0.3 mg/L and 0.9 mg/L on ammonia excretion and flesh quality. Results showed that the ammonia contents in plasma, muscle, and liver of the 9 mg/L DO group were significantly higher than those of the 3 mg/L DO group (P < 0.05). However, the expression of AMPK-related signaling pathway genes (gdh, lkb1, and ampd) and flesh quality indicators (gumminess, chewiness, hardness) in the 9 mg/L DO group were significantly lower than those in the 3 mg/L DO group. Under long-term exposure to 0.9 mg/L TAN, the ammonia contents in plasma and gill filaments, as well as muscle flesh quality (resilience, gumminess, chewiness, cohesiveness), were significantly lower than those in the 0.3 mg/L TAN group (P < 0.05). However, the activities of GDH and AMPD enzymes in the 0.9 mg/L TAN group were significantly higher than those in the 0.3 mg/L TAN group. In summary, when fish are exposed to 3 mg/L DO and 0.9 mg/L TAN in the environment for a long time, their amino acids are used for transamination and deamination, resulting in insufficient energy supply for Chinese perch, whereas 9 mg/L DO and 0.9 mg/L TAN caused deterioration of the flesh quality.

Transcriptomic analyses of Vibrio parahaemolyticus under the phenyllactic acid stress

Phenyllactic acid (PLA) generally recognized as a natural organic acid shows against Vibrio parahaemolyticus activity. In this study, V. parahaemolyticus ATCC17802 (Vp17802) was cultured under the stress of 1/2MIC PLA, and then the antibacterial mechanisms were explored via transcriptomics. The minimum inhibitory concentration (MIC) of PLA against Vp17802 was 3.2 mg/mL, and the time-kill analysis resulted that Vp17802 was inhibited. PLA was able to destroy the bacterial membrane, leading to the leakage of intracellular substances and decline of ATP levels. The RNA-sequencing analysis results indicated that 1616 significantly differentially expressed genes were identified, among which 190 were up-regulated and 1426 were down-regulated. Down-regulation of the icd2 gene in the TCA cycle mediates blockage of tyrosine metabolic, arginine biosynthesis, and oxidative phosphorylation, causing insufficient energy supply of Vp17802. Moreover, PLA could cause amino acids, metal ions, and phosphate transporters to be blocked, affecting the acquisition of nutrients. The treatment by PLA altered the expression of genes encoding functions involved in quorum sensing, flagellar assembly, and cell chemotaxis pathway, which may be interfering with the biofilm formation in Vp17802, reducing cell motility. Overall, 1.6 mg/mL PLA inhibited the growth of Vp17802 by disrupting to uptake of nutrients, cell metabolism, and the formation of biofilms. The results suggested a new direction for exploring the activity of PLA against Vp17802 and provided a theoretical basis for bacterial pathogen control in the food industry.Key points•RNA sequencing was carried out to indicate the antibacterial mechanism of Vp17802.•The icd2 gene in the TCA cycle mediates blockage of metabolic of Vp17802.•The biofilm formation has interfered with 1.6 mg/mL PLA, which could reduce cell motility and virulence.Graphical

Sustained production of gas hydrate through hybrid depressurization scheme with enhanced energy efficiency and mitigated ice blockage

Several trial productions have been conducted on marine natural gas hydrate reservoirs, demonstrating its enormous potential as an alternative energy source. Nonetheless, sustained and efficient production still remains challenging, with crucial issues on low gas production efficiency and blockages. The large amount of hydrate decomposition and resulting heat absorption can lead to prolonged subzero conditions close to the well; this can readily induce ice formation and blockage. This study aims to investigate the issue of ice blockages caused by insufficient energy supply during the recovery process. A systematic analysis of the energy consumption and ice blockage quantification at each stage of the traditional multi-stage direct depressurization and constant speed depressurization methods is conducted. A hybrid optimized scheme is proposed, which enhances energy utilization efficiency by appropriately utilizing reservoir sensible heat during the initial stage of production. Additionally, a multi-stage constant speed depressurization method is introduced in the later stages to mitigate production fluctuations and reduce blockage duration. Results showed a 69.5 % increase in energy efficiency and an average 67.1 % decline in gas blockage duration with comparable gas productivity. The proposed method could be a viable solution to mitigate challenges of icing blockages and fluctuating production during trial tests of marine hydrate reservoirs, achieving improved energy efficiency and sustainable production.

An active jet-based deployment mechanism for improved aerodynamic performance and thermal protection of reentry vehicles

As the demand for interplanetary transportation and deep space exploration missions continues to grow, deployable reentry vehicles have garnered significant attention due to their effective aerodynamic capture and deceleration capabilities, making them a focal point of research for nearly half a century. In reentry flight, traditional motor-driven deployment methods face risks and challenges such as insufficient motor energy supply, low deployment reliability, excessive weight, occupancy of payload space, and potential electromagnetic interference. If deployment failure occurs, it can significantly impact the safety of the flight mission. To address these challenges, this study proposes a jet-based deployment strategy utilizing shoulder thrusters. By carefully designing the associated parameters, this strategy offers a viable alternative to motor-driven mechanisms for deployment tasks. Furthermore, the aerodynamic simulations of the deployment process are conducted, considering various operating conditions, to investigate the influence of deployment angles on the flow field and to compare the aerodynamic performance of the two strategies. Additionally, a comparative analysis of the mass characteristics is conducted. The results show that the jet-based deployment strategy achieves desired objectives and offers advantages such as reduced drag, increased heat flux, enhanced stability, reduced mass, and improved space utilization. These findings have significant engineering implications and pave the way for widespread applications in the future.