AbstractInterest in the promotion of biofuel investments to mediate the energy crisis and assist rural development is growing across sub‐Saharan Africa. However, empirical evidence on how policy can effectively promote sustainable biofuels is largely lacking. This paper presents new, multi‐level assessments of the implications of the Malian Strategy for Biofuels Development for the promotion of the oil‐bearing, non‐edible tree Jatropha curcas (Jatropha) as a sustainable development tool in Mali: a sub‐Saharan African country that has led Jatropha‐derived biofuel policy initiatives. In doing so, the paper addresses knowledge gaps on the role of national policy instruments in the uptake of biofuel activities. Semi‐structured interviews were undertaken with government departments, international organizations, private sector representatives, non‐governmental organizations and farming communities. Data show that mainstreaming internationally agreed principles into national policies is key to attract monetary, institutional and technical support from international organizations and donors. However, there are gaps between policy targets, actual yields and land cover. Jatropha oil supplies remain insufficient for improving access to fuel and substituting national consumption. Ambitious land cover targets set within national policies could risk land use shifts away from food towards biofuel production. Improved cooperation and coordination among state departments, enhanced monitoring of programmes and projects, as well as the establishment of adequate regulatory and fiscal frameworks governing private biofuel investments are needed to achieve sustainable outcomes. Copyright © 2015 John Wiley & Sons, Ltd and ERP Environment