Institute Of Hydrobiology Research Articles

Characterization of UV-absorbing Mycosporine-like Amino Acids in Freshwater Cyanobacterium Nostoc calcicola

Mycosporine-like amino acids (MAAs) are gaining attention for their UV photoprotective properties and potential applications in biomedical fields, cosmetics, and toiletries. In this study, Nostoc calcicola a genus of cyanobacteria distributed in freshwater were tested for MAA production. This organism was provided by the Freshwater Algae Culture Collection of the Institute of Hydrobiology, the Chinese Academy of Sciences. It was isolated and cultured in the laboratory, in 500 ml glass flasks containing 300 ml of liquid medium BG11 treated on UV-B light 0.1 Wm–2. At different time, cultures were exposed to UV-B light at intensity 0.1 W. m-1 to determine the Maximum PSII photochemical (Fv/Fm). MAAs were extracted using 100% HPLC grade methanol and separated through high-performance liquid chromatography and liquid chromatography/mass spectrometry. Based on liquid chromatography/mass spectrometry analysis, one MAA compound was identified as porphyra. Thus, UV-B treatment of Nostoc calcicola significantly enhanced the production of UV-absorbing mycosporine-like amino acids (MAAs). The content of porphyra could reach up to 8.5 mg/g dry weight in Nostoc calcicola under UV-B treatments for 2 days. The present cyanobacteria could be used for biotechnology research and the UV-absorbing compound porphyra may be of great value in the development of novel sunscreens.

Assessment of the radiation exposure dose in air-water plants of various water body types in Polissia

The study aimed to determine the exposure patterns in air-water plants of Polissia reservoirs located in the territories with different levels of radionuclide contamination of the catchment area, and to assess the risk of radiation damage to air-water plants based on the radionuclides content in the bottom sediments of the reservoirs. To determine the regularities of the air-aquatic plant irradiation dose formation the results of the 2014– 2020 research were used on the specific activity of 90Sr and 137Cs in water, bottom sediments, and a set of data on the radionuclides content in aerial organs, rhizomes, and roots of Phragmites australis (Cav.) Trin as well as Tуpha angustifolia L. in the ecosystems of reservoirs outside the exclusion zone – Kyivskyi (Strakholissia village) and Povchanskyi (Povch village, Korostensky district, Zhytomyr Region) reservoirs, Bile lake (Bile village, Varasky District, Rivne Oblast), and Lake Lisove (village of Nova Markovka, Vyshgorod district, Kyiv region); in the exclusion zone – Lake Glyboke, Yanivsky Zaton and the averaged samples from the two reservoirs formed in the course of draining of the Chernobyl Nuclear Power Plant cooling reservoir. The specific activity of 90Sr in the samples was determined by the oxalate method, and 137Cs was determined by the gamma spectrometric method in the aquatic radioecology department of the Institute of Hydrobiology of the National Academy of Sciences of Ukraine. The heterogeneity of the plants environment, the attenuation of g-radiation by water masses and biomass, and the level of radionuclide contamination of aboveground and underground organs were taken into account while determining plant radiation dose rates. It was established that during the research period the dose rate of external g-irradiation of underwater organs of air-water plants that is formed by 137Cs concentrated in water masses, is 100 or more times lower than that of 137Cs radiation accumulated in bottom sediments, i.e. it can be neglected when determining the total dose. In the studied reservoirs of the exclusion zone, the external irradiation dose rate in air-water plants was 7.5–271 μGy/day, outside the exclusion zone it was 0.1–33 μGy/day. The dose rate of internal irradiation in the exclusion zone reservoirs air-water plants was 1.2–50 μGy/day, outside the exclusion zone it was 0.01–15 μGy/day. The total radiation dose rate in the exclusion zone reservoirs air-water plants was 9–289 μGy/day, in the reservoirs outside the exclusion zone it was 0.65–48 μGy/day. In the reservoirs outside the exclusion zone, the contribution of the external component to the total dose rate ranged from 69 to 81 %, in reservoirs within the exclusion zone it ranged from 69 to 78 %. The study established the levels of radionuclide content in the bottom sediments corresponding to various degrees of radiobiological effects manifestation in air-water plants. It was established that the plants of most reservoirs, in particular the Kyiv Reservoir, develop in the conditions of radiation well-being zones of and physiological masking, in some reservoirs of the exclusion zone they develop under the conditions of ecological masking zones. Key words: air-water plants, dose rate, bottom sediments, radionuclides.

Exploring cyanobacteria seed resources and corresponding gas-vesicle-biosynthetic elements for producing ultrasound molecular imaging probes

Use of gas vesicles (GVs) as biosynthetic ultrasound molecular imaging (UMI) probes has become a hot topic of research in recent years owing to their advantages of high stability, low risk of toxicity, genetic engineering characterization, easy post-modification, and drug-loading potential. Exploration of natural GV biosynthetic resources and their corresponding biosynthetic elements for various ultrasonic diagnosis and treatment needs to be developed in this field. In this study, we utilized the sequenced cyanobacteria resources preserved in the Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB) to screen seed cells and corresponding synthetic elements for biosynthesis of UMI probes. GV operons from the genomes of 158 strains of cyanobacteria were analyzed to identify their natural GV biosynthetic elements. Based on the ultrasound screening method (VINNO D860LAB, 2D transducer X6-16L, 50 % output power mode, MI: 1.116), 57 seed resources with biosynthetic UMI probe potential from 10 genera were selected from 158 strains of cyanobacteria, corresponding to 28 types of effective biosynthetic elements. These results provide a theoretical basis for future mass production of customized GVs in chassis cells and their application in UMI.

BIOTESTING OF THE ANTHROPOGENIC IMPACT ON AREAS OF THE R. ROS AND R. PROTOKA ON THE VIABILITY OF CARP FISH EGGS AND LARVAE

BIOTESTING OF THE ANTHROPOGENIC IMPACT ON AREAS OF THE R. ROS AND R. PROTOKA ON THE VIABILITY OF CARP FISH EGGS AND LARVAE

First Author Spotlight

AnnouncementFirst Author SpotlightPublished Online:07 Jun 2022https://doi.org/10.1152/ajprenal.2022.322.6.AUMoreSectionsPDF (247 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat MADELINE GAUTHIERDepartment of Physiology, University of Arizona Health Sciences Center, Tucson, ArizonaFigure 1.Madeline GauthierDownload figureDownload PowerPointMadeline Gauthier is an MD student and PhD candidate, conducting a PhD while on a leave of absence from medical school at Loyola University Chicago. She is currently completing her PhD in the Physiological Sciences Graduate Interdisciplinary Program at the University of Arizona [Photo: Fig. 1].https://doi.org/10.1152/ajprenal.00009.2022OLHA KRAVTSOVADepartment of Molecular Pharmacology and Physiology, University of South Florida, Tampa, FloridaFigure 2.Olha KravtsovaDownload figureDownload PowerPointOlha Kravtsova is a postdoctoral fellow in Dr. Alexander Staruschenko’s laboratory at the University of South Florida in the Department of Molecular Pharmacology and Physiology. She earned her PhD degree at the Institute of Hydrobiology, Kyiv, Ukraine. Her current research focuses on renal transporters, specifically in the proximal tubule. Outside of work, she enjoys workouts, traveling, and hiking [Photo: Fig. 2].https://doi.org/10.1152/ajprenal.00053.2022This article has no references to display. Download PDF Back to Top Next FiguresReferencesRelatedInformation Collections Related ArticlesSGLT2 inhibition effect on salt-induced hypertension, RAAS, and Na+ transport in Dahl SS rats 16 May 2022American Journal of Physiology-Renal PhysiologyContributions of afferent and sympathetic renal nerves to cystogenesis and arterial pressure regulation in a preclinical model of autosomal recessive polycystic kidney disease 16 May 2022American Journal of Physiology-Renal Physiology More from this issue > Volume 322Issue 6June 2022Pages i-i Crossmark Copyright & PermissionsCopyright © 2022 the American Physiological Society.https://doi.org/10.1152/ajprenal.2022.322.6.AUHistory Received 2 November 2021 Accepted 30 March 2022 Published online 7 June 2022 Published in print 1 June 2022 Metrics

An archive for the history of limnology at Verbania Pallanza in the Italian Lake District

Since 2010, work has been underway to curate and catalogue the historical documentation archive of the Verbania Pallanza section of the CNR Institute for Research on Waters, located on the shores of Lake Maggiore in the Italian Lake District. This laboratory was established during the first decade of the 1900s with the work of Marco De Marchi, and research activities intensified from 1938 onwards with the foundation of the Italian Institute of Hydrobiology. The curation of the archives dating from these earliest times to the present has been done with professional archivist technicians from the Archival Superintendence and in collaboration with researchers from the Institute. The archived documents include those from the first phase of the organization of the Institute, as well as those derived from scientific and administrative activities and exchanges with the Ministry of Education. The documents also cover activities at a second section of the Institute, located in an ancient historical residence in Varenna, on the shores of Lake Como. The archive has a photographic section, which includes a series of photographic glass plates, digitized to allow for current use, containing photos of the Institute's environments and laboratories at different times through its history. A third section of the archive consists of around 50 interviews with aquatic scientists on topics relating to research projects carried out in the past. A further section concerns the recording of about 150 seminars on environmental research carried out in the institute between 2015 and 2020. The main research topics considered concern physical, chemical and biological limnology, with particular attention to Lake Maggiore, Lake Orta (severely polluted due to industrial waste), and high-altitude lakes in the Alps. The Institute also houses a library dedicated to environmental issues and some miscellaneous papers by the most important scholars of freshwater science in Italy, with publications starting from the second half of the nineteenth century. Other collections of archival interest are a museum of field and laboratory instruments, and a collection of biological samples, mainly plankton, collected in various Italian lakes.

Protecting Cetaceans In The Yangtze

As part of my work at the Institute of Hydrobiology in Wuhan since 1982, I spent twenty years with Qi Qi [pronounced chee-chee], the world’s only captive baiji Yangtze River Dolphin (Lipotes vexillifer, “the flag-bearer who was left behind”).

Publisher’s Note to Launch Hydrobiology

When I was a student at Wuhan University, the last captive individual of baiji (Lipotes vexillifer), a species of freshwater dolphin, was living at the Freshwater Dolphin Research Centre of Wuhan Institute of Hydrobiology, Chinese Academy of Sciences [...]

Ювілей видатного гідроеколога сучасності (до 90-річчя академіка НАН України В.Д. Романенка)

November 30, 2020 marks the 90th anniversary of an outstanding Ukrainian scientist in the field of hydrobiology, hydroecology, ecological physiology of aquatic animals, winner of two State Prizes of Ukraine in Science and Technology (1995, 2004), Honored Worker of Science and Technology of Ukraine (1991), winner of the V.Ya. Yuriev Prize of the NAS of Ukraine (1984), І.І. Schmalhausen Prize of the NAS of Ukraine (2002), Director of the Institute of Hydrobiology of the NAS of Ukraine in 1980–2016, Doctor of Biological Sciences (1970), Professor (1983), Academician of the NAS of Ukraine (1988) V.D. Romanenko

Researches of the chemical composition of surface water in Ukraine, 1920-2020 (review)

The development of researches of the chemical composition of surface waters (rivers, lakes, reservoirs and ponds) is due to problems that are solved at one stage or another in the development of the country’s economy and are related to water quality issues. Depending on the tasks set during hydrochemical researches, attention is paid to a particular group of chemical components in water: 1) the main ions and their total amount (water mineralization); 2) dissolved gases; 3) biogenic elements; 4) organic matter; 5) microelements (including heavy metals); 6) radioactive elements; 7) specific pollutants. The article presents an analytical review of studies of the chemical composition of surface waters of Ukraine from the beginning of systematic research in the first half of the 20th century to the present day (1920-2020). The authors identified four typical chronological periods in the history of hydrochemical studies of surface waters in Ukraine. I period (1920s-1950s) – the beginning of systematic hydrochemical studies of surface waters; the appearance of regular observations of the chemical composition of water at the posts of hydrometeorological service on the Dnipro and Southern Bug rivers (1930s) and publication of these data in “Hydrological Yearbooks”; hydrochemical studies for selected large projects (Dnipro hydroelectric power station). II period (1950s-1970s) - expansion of hydrochemical research to meet the needs of water and hydropower construction, forecasting their possible impact on the country’s water resources; increasing the number of observation points for the chemical composition of water on large and medium-sized rivers; development of hydrochemistry of reservoirs. III period (1970s – at the beginning of the 2000s) – development of complex hydrochemical researches in the conditions of increasing anthropogenic load on water objects; creation of a system of hydrochemical monitoring of water bodies within the framework of the national system of observation and control of the environment (1973); application of sanitary and hygienic criteria for assessment of water quality – universal maximum acceptable concentrations (MAC); publication of quarterly “Hydrochemical Bulletins” (since 1967); development of radioecological studies of natural waters after the Chernobyl accident (1986); first publication in Ukraine of textbooks on hydrochemistry. IV period (after the beginning of the 2000s) – reformatting of hydrochemical research (monitoring system) to the requirements of the Water Framework Directive of the European Union, especially after the signing of the EU-Ukraine Association Agreement in 2014; reforming the water monitoring system based on environmental rationing with the identification of reference indicators; components of state monitoring of surface waters are the monitoring of biological, hydromorphological, chemical and physico-chemical parameters. The article also describes scientific hydrochemical schools: Institute of Hydrobiology of NAS of Ukraine; Taras Shevchenko National University of Kyiv; Ukrainian Hydrometeorological Institute of the State Emergency Service of Ukraine and NAS of Ukraine.

In Situ Assessment of Donghu Lake China Using Rare Minnow (Gobiocypris rarus).

In this work, rare minnow (Gobiocypris rarus) was applied as a sentinel organism and set in cages at control and test sampling sites in Donghu Lake for 4weeks in March, June, September, and December 2016 to assess the biological toxicity of in situ water. Sampling for active biomonitoring and physicochemical variables was performed weekly. The control was obtained from the outdoor pool of the Institute of Hydrobiology, China. Superoxide dismutase, lipoperoxidation, metallothioneins, acetylcholinesterase activity, and Vtg mRNA expression were determined as biomarkers during the field exposure period. Survival and growth also were monitored to evaluate the overall physiological condition of the fish. The seasonal changes of organic pollutants and trace metals (As, Hg, Cr, Cu, Zn, Cd, Pb) in surface water were determined. The integrated biomarker response (IBR) index was applied to summarize biomarker responses and correlate stress levels with concentrations of organic pollutants and trace metals in the surface water. Results indicated that complex pollution by persistent organic pollutants and heavy metals was present in Donghu Lake and that the in situ exposed organisms were stressed. Moreover, the complex pollution of Donghu Lake in summer and autumn was more serious than that in spring and winter. Active biomonitoring combined with IBR analysis enabled good discrimination among different exposure seasons. The proposed protocol with caged rare minnow revealed marked biological effects caused by the investigated Lake and a useful approach that can easily be extended to monitor water pollution.

Identification of coronavirus sequences in carp cDNA from Wuhan, China.

Severe acute respiratory syndrome (SARS)‐like coronavirus sequences were identified in two separate complementary DNA (cDNA) pools. The first pool was from a Carassius auratus (crusian carp) cell line and the second was from Ctenopharyngodon idella (grass carp) head kidney tissue. BLAST analysis suggests that these sequences belong to SARS‐like coronaviruses, and that they are not evolutionarily conserved in other species. Investigation of the submitting laboratories revealed that two laboratories from the Institute of Hydrobiology at the Chinese Academy of Sciences in Wuhan, China performed the research and submitted the cDNA libraries to GenBank. This institution is very close in proximity to the Wuhan South China Seafood Wholesale Market where SARS‐CoV‐2 first amplified in the human population. It is possible that these sequences are an artifact of the bioinformatics pipeline that was used. It is also possible that SARS‐like coronaviruses are a common environmental pathogen in the region that may be in aquatic habitats.

ОЧЕРК ИСТОРИИ ГИДРОХИМИИ ПОВЕРХНОСТНЫХ ВОД В УКРАИНЕ

The article presents an review of reserchs of the chemical composition of surface water in Ukraine from the beginning of systematic research in the first half of the twentieth century and to this day (1920-2020). Four characteristic chronological periods in the history of hydrochemical studies in Ukraine are identified. First period (1920s – 1950s) – the beginning of systematic hydrochemical studies of surface waters. Second period (1950s – 1970s) – the expansion of hydrochemical research to meet the needs of water and hydropower construction, the development of hydrochemistry of reservoirs. Third period (1970s – at the beginning of the 2000s) – development of integrated hydrochemical studies in the context of a growing anthropogenic load on water bodies; creation of a system of hydrochemical monitoring of water bodies within the framework of the national system of monitoring and environmental control. Fourth period (after the beginning of the 2000s) – reformatting of hydrochemical studies (monitoring systems) to the requirements of the European Union Water Framework Directive. The article also describes the scientific hydrochemical schools: Institute of Hydrobiology, NAS of Ukraine; Taras Shevchenko National University of Kyiv; Ukrainian Hydrometeorological Institute of the SES of Ukraine and NAS of Ukraine

«НАРИСИ ІСТОРІЇ ГІДРОХІМІЇ В УКРАЇНІ» (2020 р.) – ПЕРША МОНОГРАФІЯ ПРО ІСТОРІЮ ГІДРОХІМІЧНИХ ДОСЛІДЖЕНЬ В УКРАЇНІ

The article analyzes the monograph Essays on the History of Hydrochemistry in Ukraine (author Valentyn Khilchevskyi – professor at Taras Shevchenko National University of Kyiv), published in 2020.This is the first such monographic study. The monograph is devoted to the history of hydrochemical studies of surface waters in Ukraine. In the introductory part, an excursion on this issue was carried out in the XVIII-XIX centuries. The first part of the book is devoted to a review of hydrochemical studies of surface waters and their quality, which were carried out by Ukrainian scientists of the institutes of the National Academy of Sciences of Ukraine, industry institutions and institutions of higher education (1920-2020). As the author of the monograph notes, today scientific hydrochemical schools have been preserved at the Institute of Hydrobiology of the NAS of Ukraine (Kyiv), the Ukrainian Hydrometeorological Institute of the State Emergency Service of Ukraine and the NAS of Ukraine (Kyiv). They are engaged in hydrochemistry at the Ukrainian Institute of Environmental Problems (Kharkov). Among the higher educational institutions, it is worth noting the scientific hydrochemical school of the Taras Shevchenko National University of Kyiv, which has a prominent place in the development of the educational and methodological base, the creation of the first basic textbooks on hydrochemistry in Ukraine. They deal with individual issues of hydrochemistry at Odessa State Ecological University, Oles Honchar Dnipro National University, Yuriy Fedkovich Chernivtsi National University, Lesya Ukrainka Eastern European National University (Lutsk), National University of Water Management and Nature Management (Rivne). The second part describes the formation and history of the scientific hydrochemical school of the Taras Shevchenko National University of Kyiv (1970-2020), to which the author himself belongs. The information and facts presented in the monograph will be of extreme value from the standpoint of studying the history of hydrochemistry by young scientists, since today there is a reform in science and education, new concepts and terms are being introduced. For example, the nomenclature of the names of specialties is changing. So, the recruitment of graduate students of hydrologists-hydrochemists since 2016 is already carried out in specialty 103 “Earth Sciences” to the educational and scientific program “Hydrology”. And from 2021, it is planned to completely switch to a new form of defense of dissertations (in particular, the degree of candidate of sciences will be replaced by the degree of Doctor of Philosophy). The book will be useful to scientists and practitioners dealing with issues of hydrochemistry and water quality, teachers and students studying hydrochemical and hydroecological disciplines.

First person – Qiyu Wang

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Qiyu Wang is first author on ‘Poly-ubiquitylation of α-tubulin at K304 is required for flagellar disassembly in Chlamydomonas’, published in JCS. Qiyu is a PhD student in the lab of Kaiyao Huang in the Key Laboratory of Algal Biology, at the Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China, where he explores the role of post-translational modifications in cytoskeleton dynamics.

СОСТОЯНИЕ ЗАПАСОВ ПРОМЫСЛОВЫХ ГИДРОБИОНТОВ КИЕВСКОГО И КАНЕВСКОГО ВОДОХРАНИЛИЩ

The article analyzes the data of monitoring control catches in the Kiev and Kanev reservoirs, which are a part of the Dnieper cascade of hydroelectric stations. The analysis results allow to assess the current ecological and biological status of the populations of the main fish species in the conditions of anthropogenic impact. The analysis is based on the data of spring control catches carried out by the researchers of Institute of Hydrobiology of National Academy of Science of Ukraine in 2015. It has been stated that generally stocks of valuable commercial fish in the Kiev and Kanev reservoirs can be characterized as stable. Bream and silver bream populations of the Kanev reservoir are in good conditions in terms of reasonable exploitation of commercial core. Conditions for fattening and forming commercial core are estimated as favorable. There has been stated an increase of crucian population due to the low catches and few number of predatory fishes. Dominating commercial species in 2015 in the Kanev reservoir there were roach, bream, crucian, pike perch. There has been registered growing population of cat fish, dynamic recruit-stock of asp, and commercially abundant population of perch. To restore the commercial size of ide population it has been suggested to artificially stock the water reservoir with fish in terms of the state demand. The results of the conducted research help to predict the dynamics of fishing, to scientifically justify the ways of preserving the gene pool of natural fish populations, to take timely measures to limit the catch and further rationalization of the fishery use of the Kiev and Kanev reservoirs in order to develop the concept of stable operation of large fishery reservoirs

Manipulation of Pattern of Cell Differentiation in a hetR Mutant of Anabaena sp. PCC 7120 by Overexpressing hetZ Alone or with hetP

In the filamentous cyanobacterium, Anabaena sp. PCC 7120, single heterocysts differentiate at semi-regular intervals in response to nitrogen stepdown. HetR is a principal regulator of heterocyst differentiation, and hetP and hetZ are two genes that are regulated directly by HetR. In a hetR mutant generated from the IHB (Institute of Hydrobiology) substrain of PCC 7120, heterocyst formation can be restored by moderate expression of hetZ and hetP. The resulting heterocysts are located at terminal positions. We used a tandem promoter, PrbcLPpetE, to express hetZ and hetP strongly in the hetR mutant. Co-expression of hetZ and hetP enabled the hetR mutant to form multiple contiguous heterocysts at both terminal and intercalary positions. Expression of hetZ, alone resulted in terminally located heterocysts, whereas expression of hetP, alone produced enlarged cells in strings. In the absence of HetR, formation of heterocysts was insensitive to the peptide inhibitor, RGSGR.

Development of resources, technologies and services at the China Zebrafish Resource Center

With the rapid growth of the Chinese zebrafish community, there is an increasing demand for various types of zebrafish-related resources and technologies. The China Zebrafish Resource Center (CZRC, web: http://zfish.cn) was established at the Institute of Hydrobiology (IHB), Chinese Academy of Sciences (CAS) in 2012. Till now, CZRC has built the largest zebrafish aquaculture unit in China, organized a resource bank containing more than 1200 zebrafish lines and more than 10 000 frozen sperm samples, among which over 200 mutant and transgenic lines were generated by CZRC. CZRC has established several technical supporting platforms, such as the zebrafish husbandry and health control program of international standard, a high-efficient gene manipulation technology platform, and a stable and efficient sperm cryopreservation technology platform. The main task of CZRC is to provide different types of services to zebrafish investigators in China and worldwide, such as resource services (e.g. zebrafish lines), technical services (e.g. gene knockout) and transgenic services, consultancy services (e.g. zebrafish husbandry and health consultation), and conference services [e.g. holding regular technical training courses and biennale Chinese Zebrafish Principal Investigator Meeting (CZPM)]. After five years' development, CZRC is now recognized as one of the three major resource centers in the global zebrafish community.

Histological observation of the post-surgery healing of an incision in the Amur sturgeon Acipenser schrenckii

PDF HTML阅读 XML下载 导出引用 引用提醒 施氏鲟外科手术后切口愈合过程的组织学观察 DOI: 作者: 作者单位: 1. 中国科学院水生生物研究所, 湖北 武汉 430072;2. 中国科学院大学, 北京 100049;3. 中国水产科学研究院长江水产研究所, 湖北 武汉 430223;4. 华中农业大学水产学院, 湖北 武汉 430070 作者简介: 杨俊琳(1991-),男,硕士,研究方向为动物学.E-mail:yangjunlin19910517@163.com 通讯作者: 中图分类号: S965 基金项目: 国家973计划项目（2015B150702）. Histological observation of the post-surgery healing of an incision in the Amur sturgeon Acipenser schrenckii Author: Affiliation: 1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;4. Fisheries College, Huazhong Agricultural University, Wuhan 430070, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:通过肉眼观察和组织学方法对施氏鲟（）外科手术后不同时期的切口及周围组织进行观察，研究切口愈合过程，探讨施氏鲟组织修复能力，为术后管理提供数据支持。采用150 mg/L的MS-222对30尾2龄施氏鲟进行快速麻醉，于腹中线第3~5腹骨板处切开约5 cm的切口。采用间断缝合法对切口进行缝合，于术后4 d、7 d、14 d、28 d、42 d、56 d，通过肉眼观察和组织学方法观察切口愈合过程中的宏观和微观的变化。结果发现：术后4~7 d表皮沿切口边缘向腹腔内延伸，并与腹膜相连。切口边缘出现炎性渗出物，并有少量网状或束状的Ⅲ型胶原纤维生成。术后14~42 d切口显著愈合，红肿明显减少（<0.05），并高于周围与对照组。切口边缘出现大量的成纤维细胞聚集和新生血管，形成肉芽组织。42~56 d表皮完全愈合，与对照组无差异。肉芽组织中I型胶原含量显著增加（<0.05），成纤维细胞和血管明显减少，形成瘢痕组织。研究结果表明，施氏鲟具有较强的组织愈合能力，术后56 d切口完成愈合。14~42 d是切口愈合的主要时期，这一时期应作为术后管理关注的重点时期。相比于表皮细胞的快速迁移，真皮及皮下组织的愈合速度缓慢，是愈合过程中的限制因素。 Abstract:The Amur sturgeon () is one of the eight species of Acipenseriformes that occur in China. The range of the Amur sturgeon is mainly restricted to the middle and lower Amur River. Sometimes sturgeons enter the Ussuri River, but they rarely occur in the Sungari River. This species supports fisheries, but stocks are declining significantly because of overfishing. Developing artificial cultures could reduce market dependence on wild fish and would assist in conservation of the wild stock. Although Amur sturgeons are gonochoristic, they exhibit no apparent sexual dimorphism and have no indicators of their sex for their first 1-2 years of life. Currently, the most reliable method of determining sex and gonad maturation in Amur sturgeons at an early age is through a surgical incision and visual examination of the gonads. The eggs of artificial spawning are also commonly obtained via an abdominal incision or caesarian section. The incisions may not always heal properly; thereby, causing infections, inflammation, and ulcerations that lead to adhesions or in some cases to mortality. Thus, it is critical to close the incisions for the recovery of Amur sturgeons post-surgery. The goals of this research were to study the process of incision healing, through visual and histological evaluations of the surgical incision at different stages post-surgery, to explore the repair capability of Amur sturgeons, and to provide data for the establishment of post-surgery system management. A total of 30 2-year-old Amur sturgeons were anesthetized quickly by immersion into a tank containing a 150 mg/L solution of tricaine methanesulfonate (MS-222). Once the fish reached a surgical plane of anesthesia, a sterile surgical blade was used to make a 5 cm incision through the ventral middle skin and linea alba into the coelomic cavity. The incisions were closed with three simple interrupted stitches. Samples were taken at 4, 7, 14, 28, 42, and 56 days post-surgery. A study on the healing of the incisions was conducted, through visual assessments and histological observations. The results indicated that the epidermal cells migrated into the coelomic cavity along the edge of incision and had contact with the peritoneum at 4 to 7 days post-surgery. The inflammatory infiltrate and Type Ⅲ collagen appeared at the incision margin. The incisions healed, and redness and swelling indexes significantly decreased ( < 0.05) from 14 to 42 days post-surgery. The epidermis covered the incision, and there was an overlap between the epidermis on the front of both sides. The epidermal cell layers at the incision margin significantly increased and were greater than that around the incision and the control. A large number of fibroblasts gathered at the edge of the incision, and new capillaries were regenerated, forming granulation. From 42 to 56 days post surgery, the epidermis healed completely, exhibiting no differences from that of the control. In the granulation, type I collagen substantially increased, but fibroblasts and capillaries decreased and disappeared. A scar remained. These results indicated that the Amur sturgeon had a strong repair capability. It took 56 days to close the surgical incisions. The surgical incision healed mainly during the period from 14 to 42 days post-surgery. It took longer to complete healing of the dermis and hypodermis, compared with that of the epidermis. The healing of the dermis and hypodermis was constraint with the progress of incision closure. 参考文献 相似文献 引证文献

The effects of abscisic acid, salicylic acid and jasmonic acid on lipid accumulation in two freshwater Chlorella strains.

Sustainable renewable energy is being hotly debated globally because the continued use of finite fossil fuels is now widely recognized as being unsustainable. Microalgae potentially offer great opportunities for resolving this challenge. Abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA) are involved in regulating many physiological properties and have been widely used in higher plants. To test if phytohormones have an impact on accumulating lipid for microalgae, ABA, JA and SA were used to induce two Chlorella strains in the present study. The results showed 1.0 mg/L ABA, 10 mg/L SA, and 0.5 mg/L JA, led strain C. vulgaris ZF strain to produce a 45%, 42% and 49% lipid content that was 1.8-, 1.7- and 2.0-fold that of controls, respectively. For FACHB 31 (number 31 of the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Chinese Academy of Sciences), the addition of 1.0 mg/L ABA, 10 mg/L SA, and 0.5 mg/L, JA produced 33%, 30% and 38% lipid content, which was 1.8-, 1.6- and 2.1-fold that of controls, respectively. As for lipid productivity, 1.0 mg/L ABA increased the lipid productivity of C. vulgaris ZF strain and FACHB-31 by 123% and 44%; 10 mg/L SA enhanced lipid productivity by 100% and 33%; the best elicitor, 0.5 mg/L JA, augmented lipid productivity by 127% and 75% compared to that of controls, respectively. The results above suggest that the three phytohormones at physiological concentrations play crucial roles in inducing lipid accumulation in Chlorella.