Histological observation of the post-surgery healing of an incision in the Amur sturgeon Acipenser schrenckii

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 施氏鲟外科手术后切口愈合过程的组织学观察 DOI: 作者: 作者单位: 1. 中国科学院水生生物研究所, 湖北 武汉 430072;2. 中国科学院大学, 北京 100049;3. 中国水产科学研究院长江水产研究所, 湖北 武汉 430223;4. 华中农业大学水产学院, 湖北 武汉 430070 作者简介: 杨俊琳(1991-),男,硕士,研究方向为动物学.E-mail:yangjunlin19910517@163.com 通讯作者: 中图分类号: S965 基金项目: 国家973计划项目（2015B150702）. Histological observation of the post-surgery healing of an incision in the Amur sturgeon Acipenser schrenckii Author: Affiliation: 1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;4. Fisheries College, Huazhong Agricultural University, Wuhan 430070, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:通过肉眼观察和组织学方法对施氏鲟（）外科手术后不同时期的切口及周围组织进行观察，研究切口愈合过程，探讨施氏鲟组织修复能力，为术后管理提供数据支持。采用150 mg/L的MS-222对30尾2龄施氏鲟进行快速麻醉，于腹中线第3~5腹骨板处切开约5 cm的切口。采用间断缝合法对切口进行缝合，于术后4 d、7 d、14 d、28 d、42 d、56 d，通过肉眼观察和组织学方法观察切口愈合过程中的宏观和微观的变化。结果发现：术后4~7 d表皮沿切口边缘向腹腔内延伸，并与腹膜相连。切口边缘出现炎性渗出物，并有少量网状或束状的Ⅲ型胶原纤维生成。术后14~42 d切口显著愈合，红肿明显减少（<0.05），并高于周围与对照组。切口边缘出现大量的成纤维细胞聚集和新生血管，形成肉芽组织。42~56 d表皮完全愈合，与对照组无差异。肉芽组织中I型胶原含量显著增加（<0.05），成纤维细胞和血管明显减少，形成瘢痕组织。研究结果表明，施氏鲟具有较强的组织愈合能力，术后56 d切口完成愈合。14~42 d是切口愈合的主要时期，这一时期应作为术后管理关注的重点时期。相比于表皮细胞的快速迁移，真皮及皮下组织的愈合速度缓慢，是愈合过程中的限制因素。 Abstract:The Amur sturgeon () is one of the eight species of Acipenseriformes that occur in China. The range of the Amur sturgeon is mainly restricted to the middle and lower Amur River. Sometimes sturgeons enter the Ussuri River, but they rarely occur in the Sungari River. This species supports fisheries, but stocks are declining significantly because of overfishing. Developing artificial cultures could reduce market dependence on wild fish and would assist in conservation of the wild stock. Although Amur sturgeons are gonochoristic, they exhibit no apparent sexual dimorphism and have no indicators of their sex for their first 1-2 years of life. Currently, the most reliable method of determining sex and gonad maturation in Amur sturgeons at an early age is through a surgical incision and visual examination of the gonads. The eggs of artificial spawning are also commonly obtained via an abdominal incision or caesarian section. The incisions may not always heal properly; thereby, causing infections, inflammation, and ulcerations that lead to adhesions or in some cases to mortality. Thus, it is critical to close the incisions for the recovery of Amur sturgeons post-surgery. The goals of this research were to study the process of incision healing, through visual and histological evaluations of the surgical incision at different stages post-surgery, to explore the repair capability of Amur sturgeons, and to provide data for the establishment of post-surgery system management. A total of 30 2-year-old Amur sturgeons were anesthetized quickly by immersion into a tank containing a 150 mg/L solution of tricaine methanesulfonate (MS-222). Once the fish reached a surgical plane of anesthesia, a sterile surgical blade was used to make a 5 cm incision through the ventral middle skin and linea alba into the coelomic cavity. The incisions were closed with three simple interrupted stitches. Samples were taken at 4, 7, 14, 28, 42, and 56 days post-surgery. A study on the healing of the incisions was conducted, through visual assessments and histological observations. The results indicated that the epidermal cells migrated into the coelomic cavity along the edge of incision and had contact with the peritoneum at 4 to 7 days post-surgery. The inflammatory infiltrate and Type Ⅲ collagen appeared at the incision margin. The incisions healed, and redness and swelling indexes significantly decreased ( < 0.05) from 14 to 42 days post-surgery. The epidermis covered the incision, and there was an overlap between the epidermis on the front of both sides. The epidermal cell layers at the incision margin significantly increased and were greater than that around the incision and the control. A large number of fibroblasts gathered at the edge of the incision, and new capillaries were regenerated, forming granulation. From 42 to 56 days post surgery, the epidermis healed completely, exhibiting no differences from that of the control. In the granulation, type I collagen substantially increased, but fibroblasts and capillaries decreased and disappeared. A scar remained. These results indicated that the Amur sturgeon had a strong repair capability. It took 56 days to close the surgical incisions. The surgical incision healed mainly during the period from 14 to 42 days post-surgery. It took longer to complete healing of the dermis and hypodermis, compared with that of the epidermis. The healing of the dermis and hypodermis was constraint with the progress of incision closure. 参考文献 相似文献 引证文献