With the deepening of digital transformation, the medical service industry is facing huge innovation opportunities and challenges. This paper studies the "yae920.com" one-stop service model and discusses the impact of digital transformation on its service innovation and practice. The study first analyzed the overall impact of the digital transformation on the medical service industry, including the optimization of the service process, the improvement of the patient experience, and the improvement of the operational efficiency. Then, through the method of case study, it expounds how "yae920.com" innovates the one-stop service mode in the process of digital transformation, including the integration and optimization of functions such as online appointment registration, online consultation and consultation, electronic prescription circulation, online drug purchase and distribution and so on. The research also deeply explores the challenges and coping strategies encountered by "yae920.com" in the process of service model innovation, as well as the practical results achieved. The research results show that the digital transformation provides strong support for the innovation of the "yae920.com" one-stop service mode, and effectively improves the service efficiency and patient satisfaction. The research in this paper has certain reference value for promoting the digital transformation and service mode innovation of the medical service industry.