Innermost Row Research Articles

Mechanical properties of corrugated steel lap joint under combined compression and bending

The lap joint of a prefabricated corrugated steel structure is one of the most critical components to be considered during design. Full-scale mechanical tests under combined compression and bending (N–M) were conducted to investigate the effect of axial forces and bending moments on the mechanical behavior of lap joints. Numerical methods were used to examine the effects of different axial forces on the bending behavior of the joint. Analysis revealed that buckling of the corrugated steel plate lapped on the upper layer, located near the innermost row of bolt holes, was the failure mode of the lap joint. The yield of the bolt holes on the compression (crest) and tension (valley) sides was accelerated and postponed by the axial force, respectively. Additionally, three phases with linear, nonlinear, and linear characteristics were identified from the bending moment-rotational angle (M–θ) curves. The initial rotational stiffness of the joint was the highest and decreased gradually with increasing axial force. The residual rotational stiffness of the joint was minimal and was only minimally affected by the axial force. A greater axial force on the joint lowered the bending capacity. Compared with N = 0, when N = 1200 kN, the corresponding bending moment decreased by approximately 17.1% and 9.6% for θ = 0.02 and 0.1, respectively. An empirical formula for calculating the joint rotational stiffness (Kθ) was proposed based on the N–M–Kθ relationship. These findings provide a reference for the joint design of prefabricated corrugated steel structures.

Exploring the Slow Light Features of Lattice Shifted Twist Induced Photonic Crystal Waveguides with Ring Like Holes

In this paper, slow light features of an engineered photonic crystal waveguide are presented. The combined effect of lattice shift, lattice twist and introduction of ring-like holes (rings) in the innermost rows on the slow light features is studied. The rings are introduced in the first and second innermost rows of the waveguide. Slow light is characterized by calculating group index, second and fourth-order dispersions, and delay-bandwidth products. MIT Photonic Bands software is used for simulation. With rings in the innermost rows, a flat band of 17.71 nm is observed with a group index value of 12.72 and a flat band of 40.16 nm with a group index of 7.77. The normalized delay bandwidth product (NDBP) is observed as 0.1378 in the first structure and 0.1874 in the second structure with the proposed effects. These values are nearly 4.3 times higher in comparison with the NDBP value of the base structure (0.0436) and approximately 2 times higher than the engineered structure (0.0927). These structures are useful in slow light applications.

Effect of porosity and loading height on the performance of household LPG gas stoves

In the present work, the effect of porosity and loading height on the performance of the domestic gas burners, using LPG as fuel, is studied extensively through experimentation. Water Boiling Test (Version 4.2.3) as per the recommendations of International Organization for Standardisation International Workshop Agreement (ISO-IWA) is adopted to determine the thermal efficiency of the domestic gas stove. It is observed that, by increasing the number of holes in intermediate and innermost rows by about 96% and 73%, the pitch gets reduced by 58% and 65% respectively. Due to increased porosity, the thermal efficiency in hot and cold phase is increased by about 30% and in simmer phase the thermal efficiency is increased by about 26%. In addition to this, it is also observed that there exists an optimum height for keeping the container over the gas burner (loading height) up to which the thermal efficiency increases and beyond that, the thermal efficiency drops significantly. The optimum loading height for cold and hot phase is 14.1, and 14.6 mm respectively for the LPG cookstove used in present work (1–3 kW). The thermal efficiency at cold and hot phase, while running the gas stove with stand (11.25 mm) is reduced by about 18.5 and 16.5% respectively compared to the optimum loading height of 14.1 and 14.6 mm respectively.

Behavior of stud connections between concrete slabs and steel girders under transverse bending moment

The effect of transverse bending moment on stud connections is usually ignored in the design of steel–concrete composite bridges due to beam web flexibility. However, large transverse moment may arise near transverse stiffeners and diaphragms, because they constrain and stiffen the webs and cause tensile forces in stud connectors. Large web spacing and wide cantilevers increase these effects. This study experimentally and numerically investigates this behavior. Four groups of pull-out tests of a single stud connector under tension force are performed, and they provide insight into the behavior of stud connectors under direct tensile loading. Then four stud connections at interfaces near transverse stiffeners are tested to investigate their behavior under transverse bending moment. Numerical models of the stud connections are established to explore local behavior. The pull-out tests show that stud height greatly influences failure mode, tension strength and ultimate separation between the steel flange and the concrete slab. A tension–separation relationship is developed from the test results and used in subsequent numerical simulations. The stud connection tests suggest that longer studs can increase the bending moment and deformation capacities. Reduced longitudinal stud spacing significantly increases the stiffness, but may result in brittle failure of the concrete slab. Stud connectors should not be welded immediately over transverse stiffeners. Numerical simulations and test data compare well, and numerical results predict tensile forces in studs located near the steel web and in the region near stiffeners and other web restraint. The stud connectors in the two innermost rows on either side of a transverse stiffener provide the restraint to develop the tensile force in the studs caused by transverse bending moments. The force in a stud increases as its distance from the steel web increases and its distance from the transverse stiffener decreases. Shear–tension interaction should be checked for these connectors. Details of transverse stud arrangements with various stud heights are provided.

PM245 Effect of Obstructive Sleep Apnea Hypopnea Syndrome on Blood Pressure And C-Reactive Protein In Male Hypertension Patients

In this paper, the slow light properties of the polyatomic Photonic Crystal (PhC) which has multiple different air holes in each primitive cell are investigated. A slow light waveguide with “U-type” group index–frequency curve, which results in nearly constant group index over large bandwidth, is achieved using this new photonic crystal geometry based on the square lattice. Also, the radius and position of the innermost rows of small air holes have been modified to investigate the feasibility of controlling the dispersion relation by subtle structural modification. Numerical results demonstrate that decreasing the group velocity effectively and meanwhile maintaining a large Normalized Delay-Bandwidth Product (NDBP) can be achieved by only modifying the radius of the innermost rows of small air holes. Shifting the innermost rows of small air holes toward the waveguide core is highly beneficial to enlarge the slow light bandwidth, but it contributes nothing to the promotion of NDBP. Our results provide important theoretical basis for the potential application offered by the polyatomic photonic crystal in future optical networks.

A systematic analysis of hole size, hole-type and rows shifting on slow light characteristics of photonic crystal waveguides with ring-shaped holes

A photonic crystal waveguide embedded in silica is proposed and the effects of number of defect rows adjacent to the line-defect, number of rows shifted in transverse direction to the light propagation and the types of holes in these rows on slow light properties are investigated without changing the line-width by plane-wave expansion method. We observe that the structure with one row of ring-shaped holes exhibits better slow light properties than the structure with two and three innermost rows of ring-shaped holes when outer and inner radius of the holes are considered as free parameters. Shifting the second innermost rows of holes is found to be preferable than shifting the second innermost rows of rings. Besides, shifting the second and third innermost rows together does not make considerable enhancement on the slow light properties as shifting only the second innermost rows, no matter the shifting holes are ring-shaped holes or only holes.

Marginal Corneal Vascular Arcades

To determine the metrics of the marginal corneal vascular arcades (MCA). The MCA and filling pattern was investigated using indocyanine green dye angiography (ICGA) in the fellow eye of patients with treated unilateral keratitis. Images were acquired using a scanning laser ophthalmoscope. Five contiguous squares (100 pixels) were aligned beyond the inner row of vessels extending approximately 700 μm into the limbal region and spanning an arc length of approximately 4 mm of the peripheral cornea. Geometrical properties of the MCA were determined using programs written in a numerical computing environment. A total of 17 patients (24-88 years) were included. Filling of the inferior corneal quadrant occurred first, followed by superior, nasal, and temporal quadrants. Mean area of a vascular loop of the MCA was 11.87 × 10⁻³ mm² (SD: 10.44 × 10⁻³ mm²) skewed (2.20) toward smaller sizes. Mean circumference of a vascular loop was 422.5 μm (SD: 218.7 μm) with major and minor axes of 158.9 μm and 90.8 μm. There were five (SD: 1.8) branches per loop with a segment length of 89.5 um (SD 163.8 μm). Vessels were tortuous (mean 0.19, SD: 0.16) with a fractal number of 1.51 (0.12). There were significant differences between subjects in vessel loop area (P = 0.003) and number of branches (P = 0.002). Speed of flow was circumferential along the innermost row and measured at 0.22 mm/s in one subject. The MCA comprise a network of branched interlinked elliptical loops supporting circumferential blood flow in the corneal periphery. There was no definable change in vascular pattern extending into the limbal region.

Effects of planting design on planted seedlings and spontaneous vegetation 16 years after establishment of forest edges

In afforestation, studies of forest edges seldom span the effects of planting design and the self-designing capacities of spontaneous vegetation. This study revisited experimental forest edge sections 16 years after their establishment to assess the impact of five design elements on (1) horizontal and vertical edge structure, (2) growth of planted species and species groups, and (3) spontaneous establishment of woody vegetation. The design elements were: exposure to direct solar radiation; edge typology (shrub mixture, mixture of trees and shrubs, no edge planting); width of edge planting (5 or 10 m); species mixture principle (intimate mixtures or single species grouping); and planting row position from outermost to interior planting row (max. 6 rows). The results demonstrate that edge plantings support significantly more spontaneous woody vegetation than stand boundaries without such edge plantings. This result was consistent across the different design elements. Position across the planting rows from the outermost to innermost row was the most influential design element for the development of planted seedlings. Planted tree and shrub species of similar shade tolerance had contradicting development, where light-demanding pioneer tree species had obtained dominant positions while shade-tolerant shrub species had outgrown more light-demanding species. These results demonstrate that important successional processes can be activated by establishing the initial components and structures of forest edges. When designing such edges, species should preferably be allocated to different row cohorts reflecting their growth form and shade tolerance.

Optofluidic photonic crystal slow light coupler

A multifluid infiltrated input/output coupler for efficient light transmission from a slow light hole-type hexagonal photonic crystal waveguide (PCW) was designed based on the optofluidic technique. The structure of the coupler is similar to a regular PCW, with a little difference in the two innermost rows, which are filled with different fluids. This coupler allows light to experience a step-by-step change in its group velocity as it reaches and passes the central slow light PCW. Simulation results reveal a transmission improvement of more than 75% with the use of a three-fluid infiltrated coupler compared to a conventional PCW, in which the light is inserted directly to the slow light PCW, with a transmission efficiency of about 10%.

Localization of Papillofoveal Bundles in Primates

Axons in the fovea are precisely organized to ensure accurate vision. We investigated the morphologic characteristics and localization of nerve bundles in the optic nerve in primates. Macaque eyes were studied for conventional and immunostaining, and also marmoset eyes for carbocyanine dye tracing. Locally confined lesions associated with similar findings to human age-related macular degeneration (ARMD) were also evaluated. Axons of retinal ganglion cells formed fasciculi near their origin, and these fasciculi formed bundles thereafter. In the retinal nerve fiber layer, ascending bundles assembled stratification adding proximal bundle underneath successively. Bundles in the arcuate zone displayed a characteristic fine, parallel arrangement, whereas those in the outside zone intermingled with undefined reticular bundles as they approached the optic nerve head. Macular bundles remained in groups and were distributed in the temporal wedge of the optic nerve head. Orthograde and retrograde tracing revealed that these bundles formed confined groups of various sizes and, ultimately, a specific group of small bundles located in the innermost row, near the central vessels. In addition, these bundles showed evidence of focal degenerative deterioration in eyes with ARMD. Papillomacular bundles have a characteristic alignment and configuration. Foveal bundles that compose the confined group closest to the optic trunk (which we term papillofoveal bundles) appear to have functional significance with respect to the isolated lesions that accompany central vision loss or preservation.

Slow light engineering in polyatomic photonic crystal waveguides based on square lattice

In this paper, the slow light properties of the polyatomic Photonic Crystal (PhC) which has multiple different air holes in each primitive cell are investigated. A slow light waveguide with “U-type” group index–frequency curve, which results in nearly constant group index over large bandwidth, is achieved using this new photonic crystal geometry based on the square lattice. Also, the radius and position of the innermost rows of small air holes have been modified to investigate the feasibility of controlling the dispersion relation by subtle structural modification. Numerical results demonstrate that decreasing the group velocity effectively and meanwhile maintaining a large Normalized Delay-Bandwidth Product ( NDBP) can be achieved by only modifying the radius of the innermost rows of small air holes. Shifting the innermost rows of small air holes toward the waveguide core is highly beneficial to enlarge the slow light bandwidth, but it contributes nothing to the promotion of NDBP. Our results provide important theoretical basis for the potential application offered by the polyatomic photonic crystal in future optical networks.

Organogenesis in the budding process of the freshwater bryozoan Cristatella mucedo Cuvier, 1798 (bryozoa, phylactolaemata)

The phylogenetic position of bryozoans has been disputed for decades, and molecular phylogenetic analyzes have not unequivocally clarified their position within the Bilateria. As probably the most basal bryozoans, Phylactolaemata is the most promising taxon for large-scale phylogenetic comparisons. These comparisons require extending the morphological and developmental data by investigating different phylactolaemate species to identify basal characters and resolve in-group phylogeny. Accordingly, we analyzed the bud development and the organogenesis of the freshwater bryozoan Cristatella mucedo, with special focus on the formation of the digestive tract and differentiation of the coelomic compartments. Most parts of the digestive tract are formed as an outpocketing at the future anal side growing towards the mouth area. The ganglion is formed by an invagination between the anlagen of the mouth and anus. The lophophoral arms develop as paired lateral protrusions into the lumen of the bud and are temporarily connected by a median, thin bridge. All coelomic compartments are confluent during their development and also in the adult. The epistome coelom develops by fusion of two peritoneal infolds between the gut loop and overgrows the ganglion medially. The coelomic ring canal on the oral side develops by two lateral ingrowths and supplies the oral tentacles. On the forked canal, supplying the innermost row of tentacles above the epistome, a bladder-shaped swelling, probably with excretory function, is present in some adults. It remains difficult to draw comparisons to other phyla because only few studies have dealt with budding of potentially related taxa in more detail. Nonetheless, our results show that comparative organogenesis can contribute to phylactolaemate systematics and, when more data are available, possibly to that of other bryozoan classes and bilaterian phyla.

Delay-Time-Enhanced Flat-Band Photonic Crystal Waveguides with Capsule-Shaped Holes on Silicon Nanomembrane

A slow-group-velocity- and low-group-velocity-dispersion photonic crystal slab waveguide is designed by using capsule-shaped air holes in hexagonal lattice. The theoretical study shows that adjusting the aspect ratio of holes in the innermost rows can fine-tune the dispersion tail. The presented design can achieve nearly flat-band photonic crystal waveguides with group index of 21-36 over the normalized bandwidth (Deltaomega/ omega) of 1.38% - 0.4%. We also discuss the effects of the change in the holes' aspect ratio combined with a slight size adjustment. The group index in the range of 21-43 for normalized bandwidth of about 1.5% - 0.3% is obtained with the combination effect. The optimized designed exhibits a nearly constant group index of 21 over 22.7 nanometer bandwidth at lambda = 1.55 mum.

Neurturin, a member of the glial cell line‐derived neurotrophic factor family, affects the development of acetylcholinesterase‐positive cells in a three‐dimensional model system of retinogenesis

The glial cell line-derived neurotrophic factor (GDNF) family consists of the four ligands GDNF, neurturin (NRTN), artemin and persephin, which bind to the four co-receptors GDNF family receptor alpha1-4 and control through the activation of the receptor tyrosin kinase Ret several developmental processes. The purpose of this study was to analyse the expression and the influence of NRTN in the developing retina. We used retinospheres, a three-dimensional model system of the developing chicken retina. The expression of NRTN and the GDNF family receptor alpha 2 increased during development. Furthermore, expression was comparable in retinae and retinospheres. Analysis of signalling pathways influenced by NRTN in retinospheres showed activation of phosphatidylinositol-3 kinase and mitogen-activated protein kinase (MAPK). Activation of MAPK could be localised in cells of the innermost rows of the inner nuclear layer which were predominantly acetylcholinesterase-positive cells. Exogenous application of NRTN increased the amount of acetylcholinesterase-positive cells within the retinospheres at late culture stages. Additionally, we could show that Müller glia cells did not express the GFRalpha2 receptor and were probably not involved in NRTN signalling. Therefore, we conclude that NRTN directly participates in regulatory processes concerning the differentiation of acetylcholinesterase-positive cells in the chicken retina.

Dark‐rearing‐induced reduction of GABA and GAD and prevention of the effect by BDNF in the mouse retina

Gamma-aminobutyric acid (GABA) is an important retinal neurotransmitter. We studied the expression of GABA, glutamate decarboxylase 65 (GAD65) and GAD67 by immunocytochemistry and Western blot, in the retinas of control and dark-reared C57BL/6J black mice. This study asked three questions. First, is visual input necessary for the normal expression of GABA, GAD65 and GAD67? Second, can the retina recover from the effects of dark-rearing if returned to a normal light-dark cycle? Third, does BDNF prevent the influence of dark-rearing on the expression of GABA and GAD? At postnatal day 10 (P10), before eye opening, GABA immunoreactivity was present in the ganglion cell layer (GCL), in the innermost rows of the inner nuclear layer (INL) and throughout the inner plexiform layer (IPL) of control and dark-reared retinas. In P30 control retinas, GABA immunoreactivity showed similar patterns to those at P10. However, in P30 dark-reared retinas, the density of GABA-immunoreactive cells was lower in both the INL and GCL than in control retinas. In addition, visual deprivation retarded GABA immunoreactivity in the IPL. Western blot analysis showed corresponding differences in the levels of GAD65 but not of GAD67 expression between control and dark-rearing conditions. In our study, dark-rearing effects were reversed when the mice were put in normal cyclic light-dark conditions for 2 weeks. Moreover, dark-reared retinas treated with BDNF showed normal expression of both GABA and GAD65. Our data indicate that normal expression of GABA and GAD65 is dependent on visual input. Furthermore, the data suggest that BDNF controls this dependence.

Fine structure of the organ of attachment of the teleost, Garra gotyla gotyla (Ham)

We describe the morphology of the attachment organ (AO) of the teleost, Garra gotyla gotyla (Cyprinidae). It is located ventrally around the mouth opening and used by the species for attachment to submerged rocks in sub-Himalayan streams and rivers where it lives. The AO consists of three crescentic parts and a central callus part. Scanning electron microscopy (SEM) shows the former to possess numerous tubercles, each of which bears about 23–27 curved spines. Light microscopy shows the epidermis of the tuberculated parts to possess one type of cell arranged into 7–8 rows. Transmission electron microscopy (TEM) reveals these cells to contain abundant tonofilaments (hence called the filament cells). The epidermis of the callus part possesses the filament cells and additionally mucous cells, which are absent in the tuberculated parts. The superficial epidermis is apparently keratinized (thickness: 5–8 μm), and a part of the cells of the outer row is modified into spines. These cells show a thick plasma membrane envelope and possess mucous granules (diameter: 0.1–0.3 μm) and bundles of tonofilaments. The cells of the inner two to four rows possess similar organelles and additionally, prominent Golgi bodies and rough endoplasmic reticulum. Immunohistochemically, the cells of the outer row and the spines stain positively for cytokeratin. The cells of the innermost rows (five to eight) possess few tonofilaments and no mucous granules. It is evident that the filament cells of the mid- to upper epidermis are specialized for the production of mucous granules and tonofilaments, which is unique for the teleost epidermis concerned. It appears that the tuberculated parts with spines assist in anchorage and interlocking with the substratum, while the central callus part probably utilizes both suction and frictional mechanisms, and mucous secretion protects the spines from damage during anchorage and abrasion.

Coexistence of GAD-65 and GAD-67 with tyrosine hydroxylase and nitric oxide synthase in amacrine and interplexiform cells of the primate, Cebus apella.

The expression of glutamate decarboxylase forms, GAD-65 and GAD-67, in GABAergic cells was studied by immunocytochemistry in the retina of the New World monkey, Cebus apella. In the innermost rows of the inner nuclear layer (INL), somata that express GABA correspond to about 45% of the total number of cells in the central retina and about 25% on the periphery. Three subsets of GABAergic amacrine cells were identified along the horizontal meridian: about 5% express only GAD-65 and 40% GAD-67, and approximately 50% contain both forms of GAD. In the INL, GAD-65 immunoreactivity was detected in broad bands around strata 1, 3, and 5. GAD-67 immunoreactivity was observed throughout all strata. Somata that expressed GAD-67 exclusively stratified only in narrow bands around strata 2 and 4 of the INL and colocalized with beta2 and beta3 subunits of GABA-A receptor. Interplexiform and amacrine cells that express GABA also express tyrosine hydroxylase (TH) or nitric oxide synthase (NOS). GAD-67 colocalized with TH or NOS in presumed amacrine cells of the inner plexiform layer (IPL) and ganglion cell layer (GCL). GAD-65 was expressed in the TH- and NOS-immunoreactive interplexiform and amacrine cells, respectively. Different from what has been described in other mammals, TH and NOS were coexpressed in some neurons, indicating a partial overlap in retinal cell populations containing dopamine or nitric oxide in this primate.

Synaptic circuitry of neuropeptide-containing amacrine cells in the retina of the cane toad, Bufo marinus.

Synaptic connections of amacrine cells with substance P-like or neuropeptide Y-like immunoreactivity (SP-LI or NPY-LI) in the retina of the cane toad, Bufo marinus, were investigated using ultrastructural immunocytochemistry. The perikarya of SP-LI or NPY-LI amacrine cells were located in the innermost row of the inner nuclear layer. The synapses associated with SP-LI amacrine cells were distributed mainly in sublaminae 3 and 4 with about 10% in sublamina 1 of the inner plexiform layer. The synapses formed by NPY-LI amacrine cells were found in sublaminae 1, 2, and 4 with approximately equal frequency. Of a total of 175 SP-LI profiles, 56% were in presynaptic positions and 44% in postsynaptic positions. The synaptic inputs to SP-LI profiles predominantly derived from other unlabeled amacrine cell dendrites, and to a lesser extent, from bipolar cell terminals. The majority of synaptic outputs from SP-LI amacrine cell dendrites were directed onto unlabeled amacrine cell processes. The SP-LI profiles also made synapses onto bipolar cell terminals and formed synapses onto presumed ganglion cell dendrites. Of a total of 200 NPY-LI profiles, 48% were in presynaptic positions and 52% in postsynaptic positions. The profiles of NPY-LI amacrine cells mainly received their synaptic inputs from other unlabeled amacrine cell processes, and to a lesser extent, from bipolar cell terminals. The majority of NPY-LI amacrine cell profiles gave their synaptic outputs onto unlabeled amacrine cell dendrites, and others formed synapses onto presumed ganglion cell processes. These results suggest that these two populations of neuropeptide-containing amacrine cells in the Bufo retina are involved in different synaptic circuits.

Colocalization of glycine in substance P-amacrine cells of the larval tiger salamander retina.

The present study was performed as part of a systematic examination of glycine's coexistence with other classical transmitters and neuropeptides in neuronal populations of the larval tiger salamander retina. Substance P immunocytochemistry was combined with either glycine immunocytochemistry or autoradiography of glycine high-affinity uptake to examine whether tiger salamander substance P-amacrine cells express these glycine markers. Double-label analyses revealed two populations of substance P-amacrine cells that express glycine immunoreactivity and glycine high-affinity uptake. The large majority of double-labeled cells were situated in the innermost cell row of the inner nuclear layer, while a smaller number were located in the inner nuclear layer in the second cell row distal to the inner plexiform layer. Double-label immunocytochemistry revealed that these double-labeled cells accounted for 91.7% of substance P-immunoreactive amacrine cells. A slightly lower percentage (90.1%) of substance P-amacrine cells were found to exhibit a glycine high-affinity uptake mechanism. Substance P-amacrine cells that did not co-label for markers of glycine activity were situated in the innermost cell row of the inner nuclear layer. Substance P-immunoreactive displaced amacrine cells were not observed to co-label for either glycine immunoreactivity or glycine high-affinity uptake. The present study reveals that the large majority of substance P-amacrine cells in the larval tiger salamander retina co-express markers of glycine activity. This finding suggests a functional diversity in the population of tiger salamander substance P-amacrine cells relative to their coexisting relationship with a major inhibitory neurotransmitter.

Morphology and mosaics of VIP‐like immunoreactive neurons in the retina of the rhesus monkey

Vasoactive intestinal peptide (VIP) is a 28-amino acid peptide that has been demonstrated to reside in cells ( = VIP+ cells) of the retinae of various vertebrate species. In an attempt to study the morphology and distribution of VIP+ cells in the retina of the rhesus monkey in more detail, we subjected VIP+ cells observed in cryostat sections or wholemounts rhesus monkey retinae to a quantitative analysis. VIP+ cells were found to reside in the innermost row of the inner nuclear layer (INL) and in the ganglion cell layer (GCL) in similar numbers (estimate: 50 cells/mm2 at 6-10 mm eccentricity each) and only on rare occasions (12% of all VIP+ cells) in varying positions within the inner plexiform layer (IPL). Somata of VIP+ cells were circular and had a mean diameter of 9.1 microns. They gave rise to 1-3 main dendrites, which were usually oriented toward the IPL. Main dendrites ramified widely into thin fibers (dendritic field diameter less than = 1 mm), carrying varicose swellings. The fibers that contributed to one and the same plexus of VIP+ fibers preferred the middle third of the IPL, independent of the positions of the parent somata. A quantitative analysis of nearest-neighbour distances in the retinal wholemount preparation suggested that VIP+ cells in the GCL and in the INL might be distributed according to 2 independent mosaics. A comparison with Golgi-stained material leads to the tentative equation of VIP+ cells with the "spiny" A12 amacrine cell of Mariani ('90). Whereas the low density and large dendritic field size of VIP+ cells might suggest a more widespread function, the varicose dendritic morphology seems to be more compatible with functionally independent dendritic subunits mediating localized effects.