Inhibitory Power Research Articles

OBAT KUMUR EKSTRAK ETANOL KULIT BATANG KAYU MANIS (CINNAMOMUM VERUM J.PRESL) SEBAGAI PENGHAMBATAN PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTANS

Bad breath is caused by volatile sulfur compounds (VSCs) produced by the anaerobic bacteria Streptococcus mutans through the decomposition of food waste. This problem can be overcome with antibacterial mouthwash. Cinnamon contains essential oils, phenols, flavonoids, saponins and terpenoids, which have antibacterial properties. This study aimed to formulate a mouthwash from ethanol extract of cinnamon and evaluate its physical properties, stability, and inhibitory power against Streptococcus mutans using the Kirby-Bauer method. This research was experimental by making mouthwash using cinnamon extract with concentrations of 10% (FI), 12.5% ​​(FII), and 15% (FIII). The results of the research showed that the mouthwash preparations were brick red in color with different intensities, all preparations were homogeneous, had a pH according to standards except for FII and all preparations did not meet the required viscosity values. The stability test showed that the preparation was stable in storage except for the viscosity parameter FIII. The results of the antibacterial activity test showed that the inhibitory power of FI, FII, FIII against Streptococcus mutans bacteria was 7.33 mm, 8.50 mm, 9.33 mm respectively. The inhibitory power of FII and FIII was better than the positive control, namely total care-antibacterial mouthwash (8.33 mm).

Dobinin K Displays Antiplasmodial Activity through Disruption of Plasmodium falciparum Mitochondria and Generation of Reactive Oxygen Species.

Dobinin K is a novel eudesmane sesquiterpenoids compound isolated from the root of Dobinea delavayi and displays potential antiplasmodial activity in vivo. Here, we evaluate the antiplasmodial activity of dobinin K in vitro and study its acting mechanism. The antiplasmodial activity of dobinin K in vitro was evaluated by concentration-, time-dependent, and stage-specific parasite inhibition assay. The potential target of dobinin K on Plasmodium falciparum was predicted by transcriptome analysis. Apoptosis of P. falciparum was detected by Giemsa, Hoechst 33258, and TUNEL staining assay. The reactive oxygen species (ROS) level, oxygen consumption, and mitochondrial membrane potential of P. falciparum were assessed by DCFH-DA, R01, and JC-1 fluorescent dye, respectively. The effect of dobinin K on the mitochondrial electron transport chain (ETC) was investigated by enzyme activity analysis and the binding abilities of dobinin K with different enzymes were learned by molecular docking. Dobinin K inhibited the growth of P. falciparum in a concentration-, time-dependent, and stage-specific manner. The predicted mechanism of dobinin K was related to the redox system of P. falciparum. Dobinin K increased intracellular ROS levels of P. falciparum and induced their apoptosis. After dobinin K treatment, P. falciparum mitochondria lost their function, which was presented as decreased oxygen consumption and depolarization of the membrane potential. Among five dehydrogenases in P. falciparum ETC, dobinin K displayed the best inhibitory power on NDH2 activity. Our findings indicate that the antiplasmodial effect of dobinin K in vitro is mediated by the enhancement of the ROS level in P. falciparum and the disruption of its mitochondrial function.

Nitrite scavenging activities and reducing power capacities of ethanolic extracts of some fruits and vegetables

The use of fruits and vegetables for therapeutic purposes has been continuing for centuries. Now-a-days, fruits and vegetables are increasingly used as alternative medicines. The antioxidant activities of some fruits and vegetables used in the present study were determined by nitrite scavenging and reduction power experiments. Antioxidant capacity increased with increasing concentration. The highest nitrite scavenging activity and inhibitory power were observed in garlic extract. The least nitrite scavenging activity and reducing power were found in white grape and kiwi extracts. It was concluded that garlic extract which had the highest nitrite scavenging capacity and reducing power may be an appropriate additive in drugs and other pharmaceuticals intended for medical purposes. Bangladesh J. Bot. 53(3): 619-625, 2024 (September)

Uji Efektivitas Daya Hambat Ekstrak dan Perasan Lidah Buaya (Aloe barbadensis Miller) pada Bakteri Staphylococcus Aureus dengan Metode Difusi

Aloe vera is a plant that is rich in minerals, vitamins, and antibacterial properties. A study to analyze the antibacterial effectiveness of aloe vera in inhibiting the growth of Staphylococcus aureus bacteria isolated from acne pus. This study was an experimental laboratory study using the disc diffusion method, located at the Bacteriology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, from February to May 2024. There were 6 sample treatments with 4 replications, 4 treatments of extract and juice concentration variations, namely concentrations of 70%, 80%, 90%, 100%, positive control using penicillin antibiotics, and negative control using clean distilled water. The results of this study show that at aloe vera extract concentrations of 70%, 80%, 90%, and 100%, the average diameter of the inhibition zone was 9 mm, 10 mm, 11 mm, and 12 mm, respectively, while in aloe vera juice, these concentrations did not form an inhibition zone. So it was concluded that aloe vera extract is more effective than aloe vera juice, the most successful test material to slow the growth of Staphylococcus aureus bacteria in this study was aloe vera extract with a concentration of 100%. This study also conducted qualitative phytochemical testing on aloe vera extract which has antibacterial compounds, namely saponins, tannins, flavonoids, and terpenoids. The results of the study on the effectiveness of the inhibitory power of aloe vera extract and juice against Staphylococcus aureus bacteria using the disc diffusion method were analyzed using quantitative descriptive and presented in tabulation form in the form of the diameter of the inhibition zone formed.

INHIBITORY EFFECT OF BASE GEDE ETHANOL EXTRACT AGAINTS BACTERIAL GROWTH OF Escherichia coli

Foodborne disease is a condition in which food is contaminated by microorganisms or chemicals, causing disease. One of the microorganisms that most often causes this condition is Escherichia coli. There is an assumption which states that, base gede/base genep has antibacterial properties which inhibit the growth of bacteria in typical Balinese food such as lawar. Various studies have proven that the basic ingredients of base gede have antibacterial activity obtained from secondary metabolites. The purpose of this study was to determine the phytochemical compounds contained in the base gede and the inhibitory power of the base gede in inhibiting the growth of the Escherichia coli ATCC 25922. This study examined the content of secondary metabolites qualitatively and the experimental test was the true experimental posttest only control group design. The method used was the disk diffusion (Kirby Bauer) with variations in the concentration of ethanol extract base gede as many as four groups (25%, 50%, 75% and 100%) and the negative control group was 96% ethanol and the positive control group ciprofloxacin 5g. The results of the phytochemical test found that the base gede ethanol extract contained phenols, saponins, tannins, alkaloids, terpenoids, and flavonoids. Inhibition test results observed inhibition zones formed at concentrations of 50%, 75%, 100% and K+ with diameters respectively 5.65+/-0.65 mm ; 7.00+/-1.35mm ; 7.78+/-1.18 mm dan 34.52+/-1.49 mm. Kruskal wallis test showed a value of p = 0.001 (p <0.05) which showed that the concentration group had an effect on the inhibition of E.coli ATCC 25922. There was a significant difference between the 50% and 100% concentrations obtained from the Mann-Whitney analysis with value of p = 0.043 (p<0.05). Keywords : Inhibition test, base gede, Escherichia coli

Antibacterial Test of Endophytic Fungi of Red Ginger Rhizome (Zingiber officinale Rosc) Against Bacillus cereus

Busy activities make people prefer to consume ready-to-eat food which sometimes does not guarantee the level of cleanliness, so that cases of food poisoning often occur. Bacillus cereus is a bacteria that causes food poisoning which is usually found in rice. To overcome this problem, research was carried out to find new sources of medicine from endophytic fungi, namely fungi that live in plant tissue. The addition of clinical drugs derived from fungi has attracted interest in the discovery of new secondary metabolites derived from endophytic fungi. This research aims to (1) obtain endophytic fungal isolates isolated from red ginger rhizomes, (2) obtain endophytic fungal isolates that are antibacterial against Bacillus cereus. The method used is a laboratory experiment by randomizing and repeating the samples. The results showed that four isolates of endophytic fungi were successfully isolated from red ginger rhizomes with varying inhibitory abilities between isolates. The highest percentage of inhibitory power based on the in vitro dual culture assay was a cream colored isolate of 100 ± 0.0%.

In vitro anti-inflammatory activity of Malus × domestica var. Mela Rosa Marchigiana pulp callus extract contrasting high glucose conditions

The anti-inflammatory activity of the “Mela Rosa Marchigiana” (MRM) pulp callus ethanol Extract (MRME) was tested in different cellular models including (i) LPS-treated RAW 264.7; (ii) HUVEC exposed to short-term high Glucose (HG, 45 mM) or normal glucose (NG, 5 mM) concentrations; and (iii) HG (30 mN) and LPS-treated U937. To evaluate the anti-inflammatory effect of the extract, Nitric Oxide (NO) production was measured in RAW 264.6 cells, while IL-8, IL-1ß and MCP-1 expressions, along with modulation of some inflammation- or senescence-associated miRNAs (miR-21, miR-126, miR-17 and miR-217) were assessed in HUVECs and/or U937. MRME treatment reduced pro-inflammatory markers amount, suggesting a decreased generalised inflammatory response. Present findings indicate that MRME can contrast senescence- or HG-induced inflammation. Moreover, in HG- and LPS-induced inflammation, MRME reduced U937 monocyte activation by inhibiting mitochondrial respiration and inflammatory markers. Finally, the inhibitory potential of MRME on the digestive enzymes α-glucosidase, α-amylase and lipase activity was investigated. Our results support the idea that MRME has a positive effect on inhibition of endothelial/macrophage dysfunction under HG/senescence inflammatory conditions. Furthermore, the obtained results showed a modest inhibitory power of the extract (IC50 values: 2.98 ± 0.24, 1.77 ± 0.15, and 2.06 ± 0.31 mg/ml, respectively), which, however, could be of some help in decreasing the absorption of glucose and triglycerides.

Antibacterial activity of patiwala leaves (<em>Lantana camara</em>) ethanol extract against diabetic ulcers bacteria

Diabetic ulcers are serious complications of diabetes mellitus (DM) affecting several individuals around the world. These complications are caused by impaired blood circulation to peripheral tissue and are further exacerbated by infection due to chronic hyperglycemia, leading to tissue death. Several types of bacteria have been reported to cause infection in diabetic ulcers patients, including Staphylococcus aureus, Staphylococcus epidermis, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae, and E. coli. This indicates that there is a pressing need to develop herbal products to minimize complications. Therefore, this study aims to determine antibacterial activity of Patiwala leaves (Lantana camara) ethanol extract against bacteria that cause diabetic ulcers. The identification of bioactive compounds from the ethanol extract of patiwala leaves was carried out qualitatively and antibacterial activity using the Kirby Bauer diffusion method in triplicate. The results showed that Patiwala leaves ethanol extract with a concentration of 100 mg/mL had a strong inhibitory power against Staphylococcus aureus, Staphylococcus epidermis, and Pseudomonas aeruginosa, with values of 18 ± 0.00, 19 ± 0.00 and 18 ± 0.00 (p< 0.05), respectively. Klebsiella pneumonia and E. coli bacteria showed strong potential as inhibitors at a concentration of 200 mg/mL, with an average zone of inhibition of 19 ± 0.00 and 20 ± 0.00 (p < 0.05). Meanwhile, Proteus mirabilis and Proteus vulgaris had moderate inhibitory potential ranging from concentrations of 25-200 mg/mL, with an average of 15 ± 0.00 to 16 ± 2.00 (p < 0.05). Based on these results, Patiwala leaves ethanol extract has the potential to be developed as antibacterial in diabetic ulcers.

Effect of the Addition Red Beetroot Powder (Beta vulgaris L. Var. Rubra L) as a Feed Additive in Feed on Production Performance, Egg Yolk Cholesterol and Blood Profile of Laying Hens

The aim of this research was to evaluate the effect of addition red beetroot powder (Beta vulgaris L. Var. Rubra L) as a feed additive in feed on production performance, egg yolk cholesterol and blood profile of laying hens. The first stage of research included testing the inhibitory power of Escherichia coli, Salmonella sp, and Lactic Acid Bacteria (LAB). The materials used in the bacterial inhibition test were red beetroot pulp powder, red beetroot peel powder and red beetroot pulp and peel powder. The method used was in vitro research and the data was analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. The second stage of research is the field application of the results of the first stage research in vivo. The material used was 120 laying hens of the isa brown strain aged 30 weeks. The method used was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 6 replications, each using 5 chickens. Data were analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. Variables observed in the second stage of research included production performance, egg yolk cholesterol and blood profile of laying hens. The results of the first stage of research showed that the use of red beetroot plant powder had a very significant effect (T<0.01) on the diameter of the inhibition zone for Escherichia coli, Salmonella sp and Lactic Acid Bacteria (LAB). Red Beetroot Pulp and Peel Powder (RBPPP) has the best antibacterial power so it was applied in the second stage of research. The results of the second stage of research showed that the addition of RBPPP had a significant effect (T<0.05) on feed consumption, had a very real effect (T<0.01) on egg weight, HDP, egg mass, feed conversion, IOFC, hemoglobin., erythrocytes and hematocrit. However, there was no significant effect (T>0.05) on egg yolk cholesterol and leukocytes. The addition of RBPPP at the 1% level has the potential to reduce feed consumption and feed convertion ratio, increase egg weight, HDP and egg mass, even though the IOFC value is lower than the control treatment or without RBPPP. The addition of RBPPP at a level of 1.5 % has the potential to reduce egg yolk cholesterol levels compared to other treatments. The addition of RBPPP up to a level of 1 % is able to increase and maintain hemoglobin and hematocrit levels in normal conditions. Apart from that, the addition of RBPPP at levels of 0.5 % and 1.5 % is able to increase and maintain erythrocyte and leukocyte levels in normal conditions.

Daya Hambat Asap Cair Tongkol Jagung Terhadap Bakteri Kontaminan Pada Tuna Loin Segar Diasosiasikan Dengan Kadar Histamin Dan TVB-N

The objective of this study is to analyse the inhibitory power of liquid smoke derived from corn cobs (Zea mays L.) at different levels of distillation on bacterial contaminants in fresh tuna (Thunnus sp) loins, which are associated with histamine levels and TVB-N values. The treatment in this research was the level of liquid smoke distillation, namely 3 times distillation, 2 times distillation, and 1 time distillation. The parameters tested included the inhibitory power of liquid smoke against bacteria, histamine levels, and TVB-N values, which were tested at the 18th hour at room temperature. This research was designed using the Completely Randomized Design (CRD) method. Data analysis used ANOVA and further tested with BNT. The research results demonstrated that the level of liquid smoke distillation had a significant influence (p5%) on all test parameters. The histamine levels produced ranged from 23,68 ppm to 46,18 ppm, while the TVBN values were in the range of 7,77mgN/100g to 11,05mgN/100g. Both were still in accordance with the SNI for Fresh Tuna Loin (2018). The greater the degree of distillation of liquid smoke, the more pronounced the inhibitory effect on bacteria and the greater the potential to inhibit bacterial growth in tuna loin.

Characterization and Antioxidant Activity Assay of Essential Oil Parts of Rosella (Hibiscus sabdariffa L.)

Rosella (Hibiscus sabdariffa L.) is known as a plant whose calyx contain high levels of antioxidant bioactive compounds and good inhibitory power against free radicals. However, other parts of the rosella plant have not yet been utilized for their antioxidant content and activity. The aim of this research is to compare the profiles of essential oils of parts of the rosella plant (seeds, leaves and flower petals) and their antioxidant activity. This research method begins with the process of extracting essential oils using the water-steam distillation method. Furthermore, the profile of the essential oil is known from the results of yield calculations and characterization using the GC-MS instrument. The antioxidant activity was tested using the DPPH method. The yield of essential oils obtained was 0.0107% for seeds, 0.0087% for leaves and 0.0136% for flower petals. GC-MS characterization shows that the most abundant chemical components contained in the essential oils of seeds, leaves, and flower petals are fatty acid compounds (2-propanoic acid, butanoic acid, hexanoic acid) and esters (neopentyl isobutyrate). The results of the antioxidant activity test showed the IC50 value for each essential oil, namely, seeds: 30.15 μg/ml, leaves: 171.27 μg/ml and flower petals: 126.58 μg/ml. The compound characteristics obtained showed the best results for rosella seed essential oil, this was supported by the fairly high antioxidant activity test results IC50 <50 μg/ml. Keywords: Antioxidant activity, Characterization, Essential oils, IC50, Yield.

Formulation and Physical Quality Testing of Solid Soap From a Combination of Citronella Oil with Patchouli Leaf Oil Againts Staphylococcus aureus Bacteria

Bacteria on the skin can cause infections ranging from mild to quite serious. Bacteria that are commonly found on human skin are the pathogenic bacteria Staphylococcus aureus. The choice for making antibacterial solid soap based on active ingredients from patchouli oil and citronella oil is that this oil has inhibitory power in the growth of bacteria on the skin, one of which is Staphylococcus aureus. The aim of this research is to determine how large the inhibition zone is produced by the antibacterial solid soap formulation and to determine the characteristics and physical quality tests of the solid soap preparation combining citronella oil with patchouli leaf oil so that it is safe to use. The method used is a pure experimental method. Data collection was carried out for 4 weeks according to the testing time and then analyzed using the Graphad application. The results of the research on the inhibition zone test used 2 concentrations, namely 50% and 100%, each formulation had different average results, namely at F2 50% the average was 21 mm with a very strong category, at F2 100% the average was 19.5 mm with the strong category, at F3 50% we get an average of 16 mm in the strong category and at F3 100% we get an average of 18.6 mm in the strong category. In the tests that have been carried out, it was concluded that citronella oil and patchouli leaf oil can be made into antiseptic solid soap and can inhibit the growth of Staphylococcus aureus bacteria. The results of testing physical quality characteristics for 4 weeks have met the standard requirements for solid soap.

FORMULASI DAN EVALUASI FISIK SEDIAAN PASTA GIGI EKSTRAK DAUN BELUNTAS DENGAN VARIASI KONSENTRASI GLISERIN

Beluntas leaves (Pluchea indica) are a plant that has alkaloid, tannin and flavonoid compounds so it can be used as an antibacterial against streptococcus mutans which causes dental caries with an inhibitory power of 13.9 mm at a concentration of 5% (strong). Beluntas leaves can be developed into a toothpaste preparation. The aim of this research was to determine the formulation and physical evaluation of beluntas leaf extract toothpaste preparations with varying glycerin concentrations.Making beluntas leaf extract using the maceration method with 96% solvent ethanol ratio 1:10. Making toothpaste with varying glycerin concentrations, F1(5%), F2(7.5%), F3(10%). Physical evaluation of toothpaste preparations includes organoleptic, homogeneity, pH, high foam, spreadability and viscosity.Organoleptic tests of the three formula has a distinctive menthol odorhave the same odor, semi form solid and dark green color. The homogeneity test results F1= not homogeneous, F2= homogeneous, F3= homogeneous. Toothpaste pH test results F1 = 7.22 ± 0.10, F2 = 7.38 ± 0.12, F3 = 8.20 ± 0.35. The results of the toothpaste viscosity test were 9814.8 ± 0.55 cps, F2 = 9812.9 ± 0.59 cps, F3 =9811, 7±0.74 cps. The spreadability test results of toothpaste were F1=2.92±0.09 cm, F2=2.94±0.34 cm, F3=3.20±0.21 cm. Test results for toothpaste foam height F1 = 5.67 ± 0.67 mm, F2 = 5.67 ± 0.34 mm, F3 = 7.00 ± 0.33 mm. Based on the results of the One Way Anova test and the Kruskal Wallis test, it was concluded that variations in glycerin concentration had a significant effect on the spreadability, pH and viscosity tests.

Perbandingan Aktivitas Antibakteri Sediaan Gel dan Spray Ekstrak Etanol Biji Pepaya (Carica papaya L.)

Introduction: Acne is a psychological problem that is most frequently experienced from puberty through maturity. The papaya plant (Carica papaya L.), which has antibacterial activities present in the papaya seeds. The bioactive substances found in papaya seeds, including flavonoids, tannins, saponins, proteins, carbohydrates, tocopherols, and carotenoids, are what provide seeds the antibacterial activities Objective: This research compares the effectiveness of papaya seed ethanol extract against Propionibacterium acnes bacteria in gel and spray dose forms. . Methods: This study is an experimental lab experiment that uses 96% ethanol as a solvent and the maceration method of extraction. Results: The concentration of the gel preparation in this study was 5% (F1)with resistance diameter 15.5, namely strong antibacterial activity, 10% (F2)with an inhibitory diameter of 16.5 mm, which has strong antibacterial activity, dan 15% (F3)with an inhibitory diameter of 15.3 mm, namely strong antibacterial activity, positive control with a diameter of 27.5 mm, namely very strong antibacterial activity. On preparationspray has an inhibition zone of 0 mm, positive control 24 mm and negative control 0 mm. The resulting extract was initially tested for phytochemicals using the tube method to guarantee the quality of the extract. Organoleptic testing, homogeneity, pH, spreadability, and adhesion were among the physical qualities of gel and spray preparations that were assessed.Clindamycin served as the positive control in an antibacterial activity test against Propionibacterium acnes utilizing the well diffusion method on Muller Hinton agar (MHA) media. According to the results of the phytochemical screening, the papaya seed ethanol extract included bioactive substances as alkaloids, flavonoids, saponins, and tannins. Physical property evaluation produced results that complied with the criteria for the organoleptic, homogeneity, pH, spreadability, and adhesion tests. Conclusion: Based on this research, it can be concluded that the inhibitory power of EEBP in gel preparations is better than in gel preparation spray because the results in the gel preparation category have a stronger average.

Isolation and Identification of Lichen Photobionts Collected from Different Environments in North of Portugal and Evaluation of Bioactivities of Their Extracts.

Lichens are organisms constituted by a symbiotic relationship between a fungus (mycobiont) and a photoautotrophic partner (photobiont). Lichens produce several bioactive compounds; however, the biotechnological exploitation of this organism is hampered by its slow growth. To start studying the possibility of exploiting lichens as alternative sources of bioactive compounds, eighteen lichens were collected in the north of Portugal in order to isolate and study the bioactivity of their photobionts. It was possible to isolate and cultivate only eight photobionts. Three of them, LFR1, LFA2 and LCF3, belong to the Coelastrella genus, the other two (LFA1 and LCF1) belong to the Chlorella genus and for the remaining three photobionts, LFS1, LCA1 and LCR1, it was impossible to isolate their microalgae. These only grow in consortium with bacteria and/or cyanobacteria. All extracts showed antioxidant activity, mainly at a concentration of 10 mg.mL-1. LFS1, a consortium extract, showed the highest antioxidant power, as well as the highest concentration of phenolic compounds (5.16 ± 0.53 mg of gallic acid equivalents (GAE).g-1). The extracts under study did not show significant antibacterial activity against Escherichia coli, Listeria or Salmonella. The Coelastrella sp. and LFA1 extracts showed the highest hyaluronidase inhibition. The LFR1 extract at a concentration of 5 mg.mL-1 showed the highest anti-inflammatory activity (79.77 ± 7.66%). The extracts of Coelastrella sp. and LFA1 also showed greater antidiabetic activity, demonstrating the high inhibitory power of α-amylase and α-glucosidase. LFR1 at a concentration of 5 mg.mL-1, due to its selective cytotoxicity inhibiting the growth of cancer cells (Caco-2 cells), is a promising anticancer agent.

Nutrient content, phytochemical profile and anti-methanogen potential of Chaya (Cnidoscolus aconitifolius) leaf extracts

Abstract Chaya leaves (Cnidoscolus aconitifolius) contain many nutrients and phytochemicals expected to reduce ruminants’ methane emissions. This study aims to determine the nutritional quality, phytochemical content and potential inhibitory power of Chaya leaf extract against the growth of rumen fluid methanogen bacteria. The methods used to determine nutrient content were proximate and fibre analysis. The phytochemical content tested were total phenols, tannins and flavonoids. Anti-methanogen test through inhibitory power using liquid culture selective media for methanogens. The results showed that Chaya leaves are a source of fibre feed, which also contains high crude protein (23 - 29%). The highest total phenol and tannin content in ethanol extract of Chaya leaves is 8.62 mg/g and 31.34 ppm, respectively. In contrast, ethyl acetate extract’s highest total flavonoid content is 13.96 mg/g. The highest potential inhibitory power against methanogen bacteria was shown by ethanol extract (50 mg/mL), causing inhibition of 44.56%. This study concludes that Chaya leaves have the potential to be used as ruminant animal feed and as a suppressor of methanogen bacteria, and further tests are recommended for methane gas production in vitro.

Potensi Air Perasan Kunyit Putih, Kunyit Kuning Dan Kunyit Hitam Terhadap Bakteri Escherichia coli Penyebab Diare

Turmeric is a herbal plant that is widely used by the public (Curcuma domestica Val) which is proven to contain ingredients that can function as an antibacterial. The antibacterial properties of turmeric rhizomes are caused by its main chemical content, namely curcuminoids and essential oils. Other active substances in turmeric that can be used as antibacterials are flavonoids, alkaloids and tannins. This compound functions as an antibacterial.This research aims to explain the differences in the inhibitory power of White Turmeric, Yellow Turmeric and Black Turmeric juice against Escherichia coli bacteria. The research design used was quasi-experimental. The samples in this study were the juice of White Turmeric, Yellow Turmeric and Black Turmeric which was repeated 10 times for each treatment.Based on the research results, it is known that white turmeric rhizome juice has an average barrier potential of 33.7 mm against Escherichia coli bacteria. This is no different from the juice of Yellow Turmeric rhizomes which has an average barrier potential of 32.7 mm against Escherichia coli bacteria. Meanwhile, the juice from Black Turmeric has a smaller potential resistance compared to the juice from the rhizomes of White Turmeric and Yellow Turmeric, namely an average of 26.2 mm.

EFEKTIFITAS KOMBUCHA TEH BUNGA ROSELLA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI ESHERICHIA COLI DAN SHIGELLA DYSENTERIAE UNTUK MENCEGAH TERJADINYA DIARE

One probiotic product is kombucha tea. Kombucha tea can be combined with rosella tea. Some of the bacteria that attack the digestive tract are Escherichia Coli and Shigella dysenteriae which can cause diarrhea. It has been suggested that probiotics can be used to prevent diarrhea. This research aims to determine whether there are probiotic benefits of Rosella flower kombucha fermented for 7 days and 14 days to prevent diarrhea. Review of the growth inhibitory power of Escherichia Coli and Shigella dysenteriae bacteria. This research is in the form of Quasi-experimental. The population of this study was rosella flower kombucha. The sample for this research was rosella flower kombucha with 7 days of fermentation and 14 days of fermentation. The number of repetitions for each treatment was 16 replications, with total replications was 62 replications. The results of this research showed that the inhibitory power of rosella flower kombucha against Shigella dysenteriae bacteria with an average fermentation time of 7 days was 11.31 mm (strong), for 14 days of fermentation the average was 24.72 mm (very strong). Meanwhile, the inhibitory power of rosella flower kombucha against E. Coli bacteria with a fermentation time of 7 days averaged 11.09 mm (strong), for 14-day fermentation, the average was 20.34 mm (very strong). This research concluded that the inhibitory power of rosella flower kombucha fermented for 7 days was in the strong category and 14 days in the very strong category for both the growth of Shigella dysentriae and `E. coli bacteria.

Penilaian Efektivitas Metabolit Sekunder Trichoderma harzianum dalam Menghambat Pertumbuhan Colletotricum capsici dan Cercospora capsici : Kajian In Vitro

The cultivation of chili plants is often faced with obstacles such as disease attacks, especially anthracnose disease caused by the fungus Colletotricum capsici and leaf spot disease caused by the fungus Cercospora capsici. This study aimed to determine the effective concentration of T. harzianum secondary metabolites (MSTh) in inhibiting the growth of Colletotricum capsici and Cercospora capsici in vitro. The experiments used various MSTh concentrations: 0%, 10%, 20%, 30%, 40%, and 50%. The research results found that administration of MSTh could inhibit the growth of Colletotricum capsici and Cercospora capsici in vitro. Giving MSTh at a concentration of 10% can significantly inhibit the growth of Colletotricum capsici and Cercospora capsici compared to without MSTh. The effective concentration indicated by the percentage value of inhibitory power and colony weight reduction is an MSTh concentration of 40% with a growth inhibition of Colletotricum capsici of 89.49% and an MSTH concentration of 50% with a growth inhibition of Cercospora capsici of 57.78%.

Evaluation and Antibacterial Activity Test of Peel-off Mask Preparation from Combination of Pegagan Leaves (Centella asiatica (L) Urb.) and Charcoal Powder

Pegagan leaf (Centella asiatica (L) Urb.) is a plant that contains flavonoids, tannins, triterpenoids, and saponins that function as antibacterials. Pegagan leaf is combined with activated charcoal powder, which has the job of absorbing facial oil, so that it is formulated in the form of a peel off mask to produce a synergistic effect. This study aims to formulate, evaluate, and test the antibacterial effectiveness of the peel-off mask preparation of pegagan leaf extract at concentrations of 1.5%, 2%, and 2.5%. This study uses an experimental method by testing the parameters of the physical evaluation requirements and testing antibacterial activity with the disc method on the peel off mask preparation. The results were evaluated for 28 days, and the results of the organoleptic test were stable, homogeneity stable, pH (5.6–6.2), dry time (16–25 minutes), dispersion (6.1-6.4 cm), and irritation (2 respondents). The effectiveness test against the bacteria Propionibacterium acnes has inhibitory power of 12.3 mm in the strong category. So, it can be concluded that the peel off mask produced has stable physical characteristics and can be used as an antibacterial mask.