Perbandingan Aktivitas Antibakteri Sediaan Gel dan Spray Ekstrak Etanol Biji Pepaya (Carica papaya L.)

Abstract

Introduction: Acne is a psychological problem that is most frequently experienced from puberty through maturity. The papaya plant (Carica papaya L.), which has antibacterial activities present in the papaya seeds. The bioactive substances found in papaya seeds, including flavonoids, tannins, saponins, proteins, carbohydrates, tocopherols, and carotenoids, are what provide seeds the antibacterial activities Objective: This research compares the effectiveness of papaya seed ethanol extract against Propionibacterium acnes bacteria in gel and spray dose forms. . Methods: This study is an experimental lab experiment that uses 96% ethanol as a solvent and the maceration method of extraction. Results: The concentration of the gel preparation in this study was 5% (F1)with resistance diameter 15.5, namely strong antibacterial activity, 10% (F2)with an inhibitory diameter of 16.5 mm, which has strong antibacterial activity, dan 15% (F3)with an inhibitory diameter of 15.3 mm, namely strong antibacterial activity, positive control with a diameter of 27.5 mm, namely very strong antibacterial activity. On preparationspray has an inhibition zone of 0 mm, positive control 24 mm and negative control 0 mm. The resulting extract was initially tested for phytochemicals using the tube method to guarantee the quality of the extract. Organoleptic testing, homogeneity, pH, spreadability, and adhesion were among the physical qualities of gel and spray preparations that were assessed.Clindamycin served as the positive control in an antibacterial activity test against Propionibacterium acnes utilizing the well diffusion method on Muller Hinton agar (MHA) media. According to the results of the phytochemical screening, the papaya seed ethanol extract included bioactive substances as alkaloids, flavonoids, saponins, and tannins. Physical property evaluation produced results that complied with the criteria for the organoleptic, homogeneity, pH, spreadability, and adhesion tests. Conclusion: Based on this research, it can be concluded that the inhibitory power of EEBP in gel preparations is better than in gel preparation spray because the results in the gel preparation category have a stronger average.