Mechanism Of Inhibition Research Articles

Berberine modulates ovarian cancer autophagy and glycolysis through the LINC01123/P65/MAPK10 signaling axis

BackgroundBerberine, a readily accessible natural compound known for its ease of synthesis and low toxicity, exhibits anti-tumor properties by modulating inflammatory responses. Recent studies have revealed that berberine can also treat malignant tumors by influencing tumor metabolic reprogramming, making it a potential candidate for metabolic therapy in ovarian cancer. MethodsThe anti-proliferative and anti-metastatic effects of berberine on ovarian cancer cells were investigated using CCK-8 assays, scratch assays, EDU proliferation assays, and assays related to glycolysis and autophagy. Differentially expressed lncRNAs in ovarian cancer were identified using data from the TCGA database. A specific lncRNA's role was delineated through RNA pulldown assays, silver staining, mass spectrometry analysis, CHIP assays, and immunoprecipitation experiments, focusing on its involvement in glycolysis and autophagy regulation in ovarian cancer. Additionally, the inhibitory mechanism of berberine on ovarian cancer cells was validated through cell thermal shift assays and cycloheximide protein degradation experiments to confirm its interaction with key targets. ResultsIn vitro experiments revealed that berberine reduces glycolysis and autophagy levels, leading to the inhibition of ovarian cancer cell proliferation and metastasis. Bioinformatics analysis of TCGA data identified LINC00123 as associated with poor prognosis in ovarian cancer. Experimental validation, including RNA pulldown assays, confirmed that the LINC00123/P65/MAPK10 signaling axis regulates glycolysis and autophagy in ovarian cancer. Furthermore, at the molecular level, berberine inhibits the interaction between LINC00123 and P65, thereby reducing P65 protein stability and impeding its transcriptional regulation of downstream MAPK10. These findings were further validated in animal models. ConclusionOur study highlights berberine's dual benefits of anti-inflammatory effects and inhibition of ovarian cancer proliferation and metastasis by modulating autophagy and glycolysis levels. Mechanistically, berberine targets the LINC00123/P65/MAPK10 signaling pathway to regulate glycolysis and autophagy in ovarian cancer. These insights not only expand the potential of berberine in ovarian cancer therapy but also provide new targets and therapeutic strategies for metabolic therapy in this cancer type.

Synthesis, structural characterization, thermal analysis and anticorrosion properties of novel AZO-based imidazo[1,2-a]pyridine derivatives: Experimental study and theoretical approaches

In this study, innovative AZO-based imidazo[1,2-a]pyridine derivatives were synthesized using simple and efficient synthetic approach enabling large-scale production. Thermal analysis highlights their suitability for potential applications requiring resistance to moderate temperatures. These compounds were investigated as corrosion inhibitors for MS in a 1 M HCl-medium. Corrosion inhibition studies, including weight loss, PotentioDynamic polarization and electrochemical impedance spectroscopy methods, revealed that AZOs exhibited maximum corrosion inhibition efficiencies reaching 96.13 % and 94.07 %, respectively, at a concentration of 10−3 M and a temperature of 298 K, with both compounds acting as mixed-type inhibitors. The study also delved into the influence of temperature and immersion time on inhibitory performance, revealing stable inhibition within the temperature range of 298–328 K. The calculated standard free energy of adsorption was −42.06 and −40.62 kJ/mol for AZO 1 and AZO 2, respectively, suggesting mixed-type adsorption, with adsorption behavior following the Langmuir isotherm. Morphological analysis through SEM and AFM, showed a reduction in surface roughness in the presence of the inhibitors, while water contact angle measurements indicated a significant increase in surface hydrophobicity. UV–visible and FT-IR analyses confirmed the interaction of AZOs with the MS surface, suggesting the formation of a protective layer composed of adsorbed AZO molecules. Furthermore, computational investigations employing DFT, Monte Carlo, and Molecular Dynamic simulation revealed the formation of coordinate bonds between the studied inhibitors and the metal surface, confirming adsorption on the metallic surface. These findings align well with experimental outcomes, and a possible corrosion inhibition mechanism process was discussed.

Biological Activity of Ethanol Extract of Terminalia chebula Dried Carp against Bacterial Wilt of Lycopersicum esculentum and its Mechanism of Inhibition

Biological Activity of Ethanol Extract of Terminalia chebula Dried Carp against Bacterial Wilt of Lycopersicum esculentum and its Mechanism of Inhibition

Unveiling the Complexity of cis-Regulation Mechanisms in Kinases: A Comprehensive Analysis.

Protein cis-regulatory elements (CREs) are regions that modulate the activity of a protein through intramolecular interactions. Kinases, pivotal enzymes in numerous biological processes, often undergo regulatory control via inhibitory interactions in cis. This study delves into the mechanisms of cis regulation in kinases mediated by CREs, employing a combined structural and sequence analysis. To accomplish this, we curated an extensive dataset of kinases featuring annotated CREs, organized into homolog families through multiple sequence alignments. Key molecular attributes, including disorder and secondary structure content, active and ATP-binding sites, post-translational modifications, and disease-associated mutations, were systematically mapped onto all sequences. Additionally, we explored the potential for conformational changes between active and inactive states. Finally, we explored the presence of these kinases within membraneless organelles and elucidated their functional roles therein. CREs display a continuum of structures, ranging from short disordered stretches to fully folded domains. The adaptability demonstrated by CREs in achieving the common goal of kinase inhibition spans from direct autoinhibitory interaction with the active site within the kinase domain, to CREs binding to an alternative site, inducing allosteric regulation revealing distinct types of inhibitory mechanisms, which we exemplify by archetypical representative systems. While this study provides a systematic approach to comprehend kinase CREs, further experimental investigations are imperative to unravel the complexity within distinct kinase families. The insights gleaned from this research lay the foundation for future studies aiming to decipher the molecular basis of kinase dysregulation, and explore potential therapeutic interventions.

High temperature long-lasting corrosion inhibition mechanism of sodium dodecyldiphenyl ether disulfonate on ADC12 aluminum alloy in oxalic acid solution

The high temperature corrosion inhibition mechanism of sodium dodecyl diphenyl ether disulfonate (MADS) on ADC12 aluminum alloy in oxalic acid solution at 80 °C was investigated using electrochemical measurements, surface characterization and theoretical computations. The results manifested that MADS still had excellent performance in high-temperature oxalic acid solution, and was a highly efficient and long-lasting corrosion inhibitor with an optimal corrosion inhibition efficiency (ƞ) of 90.90 %. With increasing soaking time, the ƞ increased to 98.70 %. MADS molecule adsorbed on the aluminum alloy surface in an L-shaped walking scooter configuration through physicochemical adsorption to form a dense, thin and stable hydrophobic film, inhibiting the formation of aluminum oxalate and Al2O3, and protecting it from corrosion.

Biogenic synthesis of AgNPs via polyherbal formulation: Mechanistic neutralization and toxicological impact on acetylcholinesterase from Bungarus sindanus venom.

This study aims to examine the biogenic production, characterization, and anti-acetylcholinesterase (AAChE) properties of polyherbal formulation PHF-extract-synthesized silver nanoparticles (PHF-AgNPs). The Elapidae snake Bungarus sindanus has extremely dangerous venom for humans and contains a high amount of AChE (acetylcholinesterase). Inhibiting AChE leads to acetylcholine buildup, affecting neurotransmission. The study tested silver nanoparticles as AChE inhibitors using kinetics. Their production was confirmed through ultraviolet (UV) spectrometry at 425 nm (SPR peak of 1.94), and stabilizing functional groups were identified via Fourier transform infrared spectroscopy (FT-IR). The average length of 20 nm was confirmed by analyzing the scanning electron microscopy (SEM) data. Energy-dispersive X-ray spectroscopy (EDX) identified silver as the primary component of PHF-AgNPs (26%). Statistical analysis showed that the activity of AChE in krait venom decreased by up to 45% and 37% at a given dose of ACh (0.5 mM) by PHF and AgNPs, respectively. Utilizing the Lineweaver-Burk plot for kinetic analysis, a competitive type of inhibition is found. RESEARCH HIGHLIGHTS: Successfully synthesized PHF-extract-induced silver nanoparticles (PHF-AgNPs) demonstrated through UV spectrometry and characterized as crystalline with an average size of 45 nm by X-ray diffraction. PHF-AgNPs effectively inhibited acetylcholinesterase (AChE), an enzyme critical in neurotransmission, reducing its activity in krait venom by up to 45% at certain concentrations. Kinetic analysis revealed that the inhibition mechanism of AChE by PHF-AgNPs is competitive, offering potential for therapeutic applications in neurologically related conditions.

Prevention and control of cardiac arrhythmic by using therapeutic foods: A review.

Arrhythmia occurs as a common heart vascular disease. Functional food is a rich source of natural compounds with significant pharmacological, The aim of this paper is to explore its effect on arrhythmia. By reviewing the literature and summarising the findings, we described the role of functional foods in the alleviation of cardiac arrhythmias from different perspectives. Our study shows that functional foods have anti-arrhythmic effects through modulation of ion channels, oxidative stress, and Calmodulin-dependent protein kinase II. We summarize the mechanism of arrhythmia inhibition by the active ingredients of medicinal diets in this review article, intending to provide research ideas for dietary therapy to regulate arrhythmia.

Synergistic effects of thermally reduced graphene oxide/zinc oxide composite material on microbial infection for wound healing applications

Infections originating from pathogenic microorganisms can significantly impede the natural wound-healing process. To address this obstacle, innovative bio-active nanomaterials have been developed to enhance antibacterial capabilities. This study focuses on the preparation of nanocomposites from thermally reduced graphene oxide and zinc oxide (TRGO/ZnO). The hydrothermal method was employed to synthesize these nanocomposites, and their physicochemical properties were comprehensively characterized using X-ray diffraction analysis (XRD), High-resolution transmission electron microscopy (HR-TEM), Fourier-transform infrared (FT-IR), Raman spectroscopy, UV-vis, and field-emission scanning electron microscopy (FE-SEM) techniques. Subsequently, the potential of TRGO/ZnO nanocomposites as bio-active materials against wound infection-causing bacteria, including Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, was evaluated. Furthermore, the investigated samples show disrupted bacterial biofilm formation. A reactive oxygen species (ROS) assay was conducted to investigate the mechanism of nanocomposite inhibition against bacteria and for further in-vivo determination of antimicrobial activity. The MTT assay was performed to ensure the safety and biocompatibility of nanocomposite. The results suggest that TRGO/ZnO nanocomposites have the potential to serve as effective bio-active nanomaterials for combating pathogenic microorganisms present in wounds.

Investigating the inhibition of xanthine oxidase by five catechins: Kinetic studies, spectroscopy, molecular docking, and dynamics simulations

Catechins compounds from tea have demonstrated significant inhibitory effects on xanthine oxidase (XOD). However, the precise inhibitory mechanisms of the main catechins on XOD remain to be fully elucidated. This study explored the inhibition mechanisms and binding characteristics of five catechins (GC, EGC, EC, EGCG, and ECG) on XOD through a combination of inhibition kinetics, multi-spectroscopy analysis, molecular docking, and dynamics simulations. Among the catechins, EGCG and ECG exhibited the most potent inhibitory activities against XOD. All five catechins were found to exhibit mixed inhibition, affecting the hydrophobic groups and secondary structure of XOD predominantly through hydrophobic interactions and hydrogen bonding. Molecular dynamics simulations revealed that a 3,4,5-trihydroxybenzoic acid moiety at C3 position significantly enhances the binding affinity of EGCG and ECG to XOD. Additionally, the decrease of β-sheet and random coil induced by EGCG and ECG was found to be crucial for enhancing inhibitory activity of XOD. In vitro cell experiments showed that EGCG and ECG significantly reduced high uric acid levels of BRL-3A cell. This study elucidates the inhibitory mechanisms of catechins on XOD, paving the way for their application as XOD inhibitors to combat hyperuricemia.

Exploring the anti-NSCLC mechanism of phillyrin targeting inhibition of theHSP90-AKT pathway.

Phillyrin (PHN), derived from the dried fruit of Forsythia suspensa (Thunb.) Vahl, is a kind of Chinese herbal medicine with the effect of clearing heat, and has been used in China for thousands of years in treating various tumors. However, the mechanism of its main components on non-small cell lung cancer (NSCLC) remains unclear. PHN is a distinct component extracted from Forsythia suspensa with promising anti-cancer activity against various tumor types. This study sought to elucidate the promising effects of PHN on NSCLC. Based on network pharmacology results, we identified potential PHN targets and pathways for NSCLC treatment. CCK-8 assay, wound healing assay, apoptosis assay, western blot, and in vivo experiments verified the inhibitory effect of PHN on NSCLC. Network pharmacology identified 160 potential PHN targets, 955 NSCLC-related targets, and 54 common targets, along with 132 pathways and 2 core genes. Biological experiments demonstrated that PHN significantly inhibited the growth and migration of A549 and LLC cells while promoting their apoptosis. Western blot analysis revealed down-regulation of AKT, HSP90AA1, and CDC37 expression, suggesting that PHN inhibits A549 and LLC cell proliferation by down-regulating the HSP90-AKT pathway. In vivo experiments confirmed that PHN significantly inhibited NSCLC growth with low toxicity. This study, using network pharmacology and biological experiments, verified the effectiveness of PHN against NSCLC through the HSP90-AKT pathway. These findings provide a foundation for further research and analysis.

The Impact of Amphimedon chloros Ethyl Acetate Extract on MDA-MB-231 Cell Lines' Expression of Bax, Cyclin D, Caspase 3, P21, C-myc, and Bcl2: Mechanism of inhibition of the Growth of Cancer Cells

The Impact of Amphimedon chloros Ethyl Acetate Extract on MDA-MB-231 Cell Lines' Expression of Bax, Cyclin D, Caspase 3, P21, C-myc, and Bcl2: Mechanism of inhibition of the Growth of Cancer Cells

Inhibitory effect of caffeic acid and dihydrocaffeic acid on the formation of advanced glycation end products: Mechanism analysis based on intermolecular interaction

The accumulation of advanced glycation end products (AGEs) generated from glycation reaction accelerates the development and deterioration of some diseases. Herein, the AGEs model of fructose-induced glycation of human serum albumin (HSA) was constructed and characterized. Both caffeic acid (CA) and dihydrocaffeic acid (DHCA) can inhibit the formation of AGEs, and the inhibitory effect of CA was more pronounced, as confirmed by fluorescence spectroscopy, gel electrophoresis, scanning electron microscopy, and carbonyl and thiol contents analysis. The binding of CA and DHCA to HSA was investigated by fluorescence spectroscopy and molecular docking. The primary inhibitory mechanism was the occupation of the glycation site LYS199 of HSA, and the more significant inhibitory effect of CA was related to its stronger binding affinity. Furthermore, the conformational changes caused by glycation and CA/DHCA binding were monitored using fluorescence, circular dichroism, and dynamic light scattering analysis. The results would help to elucidate the inhibitory mechanism of CA and DHCA on AGEs formation based on their different structures and inhibitory effects, and provide guidance for choosing natural inhibitors for diabetes.

Antifungal activity of 2-chloro-5-trifluoromethoxybenzeneboronic acid and inhibitory mechanisms on Geotrichum candidum from sour rot Xiaozhou mustard root tuber

Xiaozhou mustard (Brassica napiformis) root tuber, a traditional fermented vegetable, has a long history in Rongan County, Guangxi Province. However, the frequent occurrence of root tuber sour rot by Geotrichum candidum (G. candidum) has seriously reduced Xiaozhou mustard production and quality in recent years. The objective of the present study is to investigate the antifungal efficacy of 2-chloro-5-trifluoromethoxybenzeneboronic acid (Cl-F-BBA) against G. candidum and its possible mechanisms. The results revealed that a concentration of 0.25 mg/mL Cl-F-BBA completely halted mycelial growth and spore germination. Furthermore, a slightly lower concentration of 0.20 mg/mL was sufficient to compromise the integrity of the plasma membrane in mycelia and mitochondria, leading to a reduction in respiratory rate, activities of malate dehydrogenase (MDH), and succinate dehydrogenase (SDH), ATP content, and energy charge. This concentration also significantly disordered antioxidant metabolism, resulting in the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), and caused intracellular leakage in mycelia. In vivo experiments further demonstrated that Xiaozhou mustard root tubers treated with Cl-F-BBA exhibited markedly lower decay rates and lesion diameters compared to the control group. In summary, Cl-F-BBA presents a promising solution for controlling root tuber sour rot in Xiaozhou mustard caused by G. candidum.

Unlocking the power of Dryopteris filix mas extract: A green solution for corrosion inhibition in A210Gr carbon steel in acidified 3% wt. NaCl environment

This study investigates the corrosion inhibition effectiveness of Dryopteris filix-mas L. (DFM) extract for A210Gr carbon steel in a 3 % NaCl solution. The DFM ethanolic extract was successfully prepared and comprehensively characterized using a combination of spectroscopic and physicochemical techniques, including HPLC, FTIR, and NMR (1H and 13C). These analyses confirmed the presence of key phenolic compounds, with Quercetin-3-O-diglucoside (Q3ODG) being the major component, comprising 82.14 % peak area as identified by HPLC. The extract, obtained via an economical ethanol extraction method, exhibited a maximum inhibition efficiency up to 88 % at a concentration of 400 ppm, as determined through electrochemical impedance spectroscopy (EIS), open circuit potential (OCP), potentiodynamic polarization (PDP), and weight loss measurements. Temperature studies revealed a drop in efficiency to 70.14 % at 323 K, indicating a temperature-sensitive inhibition mechanism. Extended immersion times led to a slight decrease in efficiency due to partial desorption, though significant protection remained after 72 h. The primary corrosion inhibition mechanism was determined to be physisorption, following the Langmuir isotherm model, with a ΔGads0 value of − 24.02 kJ/mol, indicating a spontaneous adsorption process. Thermodynamic analysis revealed an increase in Ea from 22.11 kJ/mol (blank) to 56.61 kJ/mol (400 ppm), confirming the formation of a strong protective barrier. Surface characterization techniques, including FTIR, XRD, SEM, and AFM, further confirmed the presence of a protective film rich in polar organic compounds. Complementary Density Functional Theory (DFT) and Quantum Theory of Atoms in Molecules (QTAIM) studies provided detailed insights into the molecular interactions, highlighting the key role of hydrogen bonding and van der Waals forces in the adsorption of Q3ODG onto the steel surface. These findings underscore the potential of DFM extract as an effective and sustainable corrosion inhibitor, advancing the application of biocompounds in corrosion control and encouraging further exploration of their industrial applications and environmental benefits.

Discovery and evaluation of novel SHIP-1 inhibitors

Src Homology 2-containing Inositol 5′-Phosphatase-1 (SHIP-1), encoded by INPP5D, has been identified as an Alzheimer’s disease (AD) risk-associated gene through recent genetic and epigenetic studies. SHIP-1 confers AD risk by inhibiting the TREM2 cascade and reducing beneficial microglial cellular processes, including phagocytosis. While several small molecules have been reported to modulate SHIP-1 activity, their limited selectivity and efficacy in advanced models restricted their potential as therapeutic agents or probes for biological studies. Herein, we validated and implemented a high-throughput screening platform to explore new chemotypes that can modulate the phosphatase activity of SHIP-1. We screened 49,260 central nervous system (CNS)-penetrate compounds sourced from commercial vendors using the malachite green-based assay for anti-SHIP-1 activity. Through analysis, prioritization, and validation of the screening hits, we identified three novel types of scaffolds that inhibit the SHIP-1 phosphatase activity with IC50s as low as 46.6 µM. To improve the inhibitory activity of these promising hits, we carried out structure–activity relationship (SAR) studies, resulting in a lead molecule SP3-12 that inhibits SHIP-1 with an IC50 value of 6.1 μM. Kinetic analyses of SP3-12 revealed that its inhibition mechanism is competitive, with a Ki value of 3.2 µM for SHIP-1 and a 7-fold selectivity over SHIP-2. Furthermore, results from testing in a microglial phagocytosis/cell health high content assay indicated that SP3-12 could effectively activate phagocytosis in human microglial clone 3 (HMC3) cells, with an EC50 of 2.0 µM, without cytotoxicity in the dose range. Given its potency, selectivity, and cellular activity, SP3-12 emerges as a promising small molecule inhibitor with potential for investigating the biological functions of SHIP-1.

Insights into inhibitory action and interaction of bisdemethoxycurcumin on tyrosinase: Spectroscopic and docking analysis

Bisdemethoxycurcumin (BDMC), a demethoxy derivative of curcumin, has garnered interest for its potential anti-tyrosinase properties, which are crucial for applications in food preservation and cosmetic industries. Despite its recognized bioactive effects, the detailed inhibitory action and interaction of BDMC on tyrosinase and its application in anti-browning processes remain unclear. This study aims to dissect the molecular interactions of BDMC with tyrosinase, elucidate its inhibition mechanism, and assess its efficacy in preventing browning, thereby underpinning its potential as a natural anti-browning agent. Employing a battery of spectroscopic techniques, we characterized the interaction of BDMC with tyrosinase. The anti-browning properties of BDMC were evaluated on fresh-cut apples, and its effect on enzymatic activities related to browning was also investigated. The results indicate that BDMC interacts with tyrosinase in a reversible mixed-type inhibition manner with an IC50 value of 12.5 ± 0.2 μM. Surface Plasmon Resonance (SPR) results indicated that BDMC presented good binding affinity toward tyrosinase. Fluorescence quenching results showed that BDMC could quench the fluorescence of tyrosinase in a static process. Synchronous fluorescence, circular dichroism spectra, and three-dimensional fluorescence results indicated that interaction of BDMC against tyrosinase resulted in changes of tyrosinase on microenvironment and conformation, thus causing decrease of tyrosinase activity. Copper-chelating results presented that BDMC could chelate to copper with stoichiometric ratio of 1: 2. Molecular docking provided intricate details of how BDMC interacted with tyrosinase. Moreover, BDMC exhibited anti-browning capability on fresh cut apples, and could regulate PPO, POD, and APX activities. The comprehensive analysis proposed in this study consolidated the theoretical basis of BDMC as a tyrosinase inhibitor and supported its potential anti-browning application.

The fine structural changes of phenolic hydroxyl groups of cinnamic acid and its derivatives affects their inhibition mechanism and interaction with α-glucosidase

Cinnamic acid (CCA), p-coumaric acid (PCA), and caffeic acid (CA) are natural phenolic compounds and extensively utilized in the food industry. PCA and CA are both derivatives of CCA and share comparable molecular structures. In this study, the inhibitory mechanisms of CCA, PCA, and CA on α-glucosidase was investigated by employing inhibition kinetics and molecular docking analyses. Our results revealed that CCA (IC50 = 6.12 ± 0.056 mM) and CA (IC50 = 2.02 ± 0.083 mM) exhibited mixed-type inhibition, while PCA (IC50 = 3.82 ± 0.095 mM) exhibited non-competitive inhibition. The intermolecular energy values of CCA, PCA, and CA with α-glucosidase were determined to be −7.11, −7.94, and −8.14 kcal/mol, respectively, indicating their binding to α-glucosidase via hydrophobic interactions and hydrogen bonding, among which CA has the greatest binding ability. Fluorescence quenching results and circular dichroism (CD) spectra further confirmed the higher affinity of CA to α-glucosidase than that of CCA and PCA. These outcomes shed light on the importance of molecular structure differences in α-glucosidase inhibition and highlight the potential scope for developing low-glycemic-index functional foods.

Computational Insights Into the Mechanism of EGCG's Binding and Inhibition of the TDP-43 Aggregation.

Misfolding and aggregation of TAR DNA-binding protein, TDP-43, is linked to devastating proteinopathies such as ALS. Therefore, targeting TDP-43's aggregation is significant for therapeutics. Recently, green tea polyphenol, EGCG, was observed to promote non-toxic TDP-43 oligomer formation disallowing TDP-43 aggregation. Here, we investigated if the anti-aggregation effect of EGCG is mediated via EGCG's binding to TDP-43. In silico molecular docking and molecular dynamics (MD) simulation suggest a strong binding of EGCG with TDP-43's aggregation-prone C-terminal domain (CTD). Three replicas, each having 800 ns MD simulation of the EGCG-TDP-43-CTD complex, yielded a high negative binding free energy (ΔG) inferring a stable complex formation. Simulation snapshots show that EGCG forms close and long-lasting contacts with TDP-43's Phe-313 and Ala-341 residues, which were previously identified for monomer recruitment in CTD's aggregation. Notably, stable physical interactions between TDP-43 and EGCG were also detected invitro using TTC staining and isothermal titration calorimetry which revealed a high-affinity binding site of EGCG on TDP-43 (Kd, 7.8 μM; ΔG, -6.9 kcal/mol). Additionally, TDP-43 co-incubated with EGCG was non-cytotoxic when added to HEK293 cells. In summary, EGCG's binding to TDP-43 and blocking of residues important for aggregation can be a possible mechanism of its anti-aggregation effects on TDP-43.

Study on cooling efficiency and mechanism of lithium-ion battery thermal runaway inhibition by additives enhanced water mist based on boiling heat transfer theory

In recent years, fire and explosion accidents caused by thermal runaway (TR) of lithium-ion batteries (LIB) have occurred frequently, which has become one of the main obstacles restricting its development. Therefore, it is urgent to develop fire extinguishing agents with high cooling efficiency to inhibit the growth of TR. The water mist is an effective choice, but it shows poor performance with the rise in cell temperature under TR. In this study, two additives, KCl and the nonionic fluorocarbon surfactant FC-4430, were added to the water mist to improve the cooling efficiency. In addition, the inhibition effect exerted by the modified water mist during the whole process of lithium-ion battery TR was experimentally investigated. Furthermore, the influence of water mist with additives on the Leidenfrost effect in the high temperature range was deduced, and the reinforcement cooling mechanism was analyzed by heat transfer theory. The results show that KCl and FC-4430 can increase the initial cooling rate in the high temperature range by 48.6%–120%. The reinforcement mechanism is attributed to changing the medium's physical parameters with additives. On the one hand, the Leidenfrost point of the droplets on the cell surface is increased, which in turn extends the temperature range of the nucleate boiling stage, and on the other hand, the heat flux density and heat transfer of the water mist in the film boiling state are increased.

Preparation of zwitterionic polymer and its application for dual-functional inhibition in deepwater drilling

During deep-water drilling operations, due to the conditions with low temperature and high pressure in the wellbore, methane hydrate can be easily formed in the presence of methane and plug the wellbore. In addition, the deep-water sediments are dominated by clay-silty with weak cementation, so well instability may be occurred due to clay hydration. In order to inhibit hydrate formation and clay hydration in deepwater drilling, a zwitterionic polymer AADV was synthesized through quaternary copolymerization of AM/AMPS/DMDAAV/VAC. The hydrate inhibition performance of AADV was evaluated in a high-pressure reactor chamber, and the results showed that AADV can delay the hydrate formation time from 330 min in pure water to 2204 min, indicating an excellent inhibition effect of hydrate formation. Meanwhile, 1 % AADV can significantly reduce the linear swelling rate of the calcium-based bentonite to 3.69 % for 10 h and increase the rolling recovery rate to 94.7 %, suggesting an excellent inhibition effect on clay hydration. The mechanism of dual-functional inhibition of AADV was further analyzed. The zwitterionic group in AADV and the hydrophilic amide group can strongly adsorb the water molecules through a strong hydration effect and the hydrogen bonding effect, respectively, which contributes to destroy the water molecules cage structure, and thus to suppress the hydrate formation. Besides, AADV can effectively obstruct mass transfer of methane molecules by increasing the viscosity of the aqueous phase and absorbing on the hydrate surface, leading to hydrate formation inhibition. For the inhibition of clay hydration, the SEM and XRD results proved that AADC inhibits the clay hydration through the film-forming effect. In addition, the contact angle of Na-Bent/AADC composite reached to 55.68° when the polymer concentration increased to 2 %, suggesting that the hydrophobicity of Na-Bent surface can be enhanced after AADC treatment. AADV can wrap around clay particles and form hydrophobic film through adsorption and intercalation, preventing the intrusion of external fluids and inhibiting clay hydration.