INHIBIT Logic Research Articles

Rhodamine B-Quinoline based schiff base as fluorescent ‘turn on’ sensor of Al3+, Cr3+, HSO4− and its cytotoxicity and cell imaging application on TPC-1 and HtH-7 cell lines

A designed Rhodamine-B-quinoline based Schiff base chemosensor (RhBQ) has been synthesized and characterized using various spectroscopic techniques. It shows ‘turn on’ fluorescence emission and also colorimetric sensitivity and selectivity towards trivalent metal ions (Al3+ and Cr3+) and anion (HSO4−) present in aqueous medium. Stoichiometry of both metal (Al3+ & Cr3+) - RhBQ complexes are found to be 1:1 using Job’s plot. A notable enhancement in emission intensity for RhBQ-Al3+ (∼341 fold), RhBQ-Cr3+ (∼292 fold), and RhBQ-HSO4− (∼136 fold) complexes compare to RhBQ are observed. Sensing mechanism i.e. opening of spirolactam ring of RhBQ has been confirmed through various spectroscopic techniques such as; 1H NMR, 13C NMR, ESI-MS, TRPL, FT-IR, and DFT based computational study. The limit of detection (LOD) for Al3+, Cr3+, and HSO4− are found to be 2.2 × 10−8 M, 2.12 × 10−8 M and 8.63 × 10−7 M respectively. An advanced level 3 input OR–INHIBIT logic gate are constructed using chelating ligand EDTA. For onsite detection, TLC paper strip spot of the probe, RhBQ is prepared and the detection of Al3+ in few antacid drug are presented. In addition, cell-imaging analysis of Al3+, Cr3+, and HSO4− ions in the Human papillary thyroid carcinoma cell line (TPC-1) & Human anaplastic thyroid cancer cell lines (HtH-7) are presented and shows promising concentration and time-dependent detection results.

Design and Synthesis of a Novel ICT Bichromophoric pH Sensing System Based on 1,8-Naphthalimide Fluorophores as a Two-Input Logic Gate and Its Antibacterial Evaluation.

The synthesis, sensor activity, and logic behavior of a novel 4-iminoamido-1,8-naphthalimide bichromophoric system based on a "fluorophore-receptor" architecture with ICT chemosensing properties is reported. The synthesized compound showed good colorimetric and fluorescence signaling properties as a function of pH and proved itself as a promising probe for the rapid detection of pH in an aqueous solution and base vapors in a solid state. The novel dyad is able to work as a two-input logic gate with chemical inputs H+ (Input 1) and HO- (Input 2) executing INHIBIT logic gate. The synthesized bichromophoric system and the corresponding intermediates demonstrated good antibacterial activity toward Gram (+) and Gram (-) bacteria when compared with the Gentamycin standard.

Valorised polypropylene waste based reversible sensor for copper ion detection in blood and water

Heavy metals and plastic pollutants are the two most disastrous challenges to the environment requiring immediate actions. In this work, a techno-commercially feasible approach to address both challenges is presented, where a waste polypropylene (PP) based reversible sensor is produced to selectively detect copper ions (Cu2+) in blood and water from different sources. The waste PP-based sensor was fabricated in the form of an emulsion-templated porous scaffold decorated with benzothiazolinium spiropyran (BTS), which produced a reddish colour upon exposure to Cu2+. The presence of Cu2+ was checked by naked eye, UV–Vis spectroscopy, and DC (Direct Current) probe station by measuring the current where the sensor's performance remained unaffected while analysing blood, water from different sources, and acidic or basic environment. The sensor exhibited 1.3 ppm as the limit of detection value in agreement with the WHO recommendations. The reversible nature of the sensor was determined by cyclic exposure of the sensor towards visible light turning it from coloured to colourless within 5 min and regenerated the sensor for the subsequent analysis. The reversibility of the sensor through exchange between Cu2+- Cu+ was confirmed by XPS analysis. A resettable and multi-readout INHIBIT logic gate was proposed for the sensor using Cu2+ and visible light as the inputs and colour change, reflectance band and current as the output. The cost-effective sensor enabled rapid detection of the presence of Cu2+ in both water and complex biological samples such as blood. While the approach developed in this study provides a unique opportunity to address the environmental burden of plastic waste management, it also allows for the possible valorization of plastics for use in enormous value-added applications.

Synthesis of Fluorophore Based Functional Material for Selective Detection of Al3+ Ion in Water and Decoding the AIEE Property of Its Hydrosol.

A designed aggregation-induced emission enhancement (AIEE) active fluorescence probe 2,3-Bis-[(2-hydroxy-napthalen-1-ylmethylene)-amino]-but-2-enedinitrile (L) was synthesized via one step condensation method. The probe shows swift sensitivity and selectivity toward Al3+over other relevant metal ions and also exhibits significant AIEE phenomena in methanol/water mixture. Significant enhancement of fluorescence intensity is triggered via chelation-enhanced fluorescence through complex (Al3+-L) formation. A 2:1 metal to ligand ratio is observed from Job's plot based on UV - Vis absorption titration and detection limit (LOD) is found as low as 31.14nM. Moreover, 1H NMR titrations and fluorescence reversibility by adding Al3+ and EDTA sequentially had been performed to establish the binding site of sensor complex (Al3+-L). Time-resolved photoluminescence, dynamic light scattering, optical microscopy, and on-site visualization studies have been performed to understand the AIEE mechanism of L in different volume percentage of water and methanol mixture. An INHIBIT molecular logic gate has been constructed utilizing the fluorescence behavior of the probe, L in presence of Al3+ and strong chelating ligand EDTA.

Integrating CRISPR-Cas12a with catalytic hairpin assembly as a logic gate biosensing platform for the detection of polychlorinated biphenyls in water samples

Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that cause harmful effects on environmental safety and human health. There is an urgent need to develop an intelligent method for PCBs sensing. In this work, we proposed a logic gate biosensing platform for simultaneous detection of multiple PCBs. 2,3′,5,5′-tetrachlorobiphenyl (PCB72) and 3,3′,4,4′-tetrachlorobiphenyl (PCB77) were used as the two inputs to construct biocomputing logic gates. We used 0 and 1 to encode the inputs and outputs. The aptamer was used to recognize the inputs and release the trigger DNA. A catalytic hairpin assembly (CHA) module is designed to convert and amplify each trigger DNA into multiple programmable DNA duplexes, which initiate the trans-cleavage activity of CRISPR/Cas12a for the signal output. The activated Cas12 cleaves the BHQ-Cy5 modified single-stranded DNA (ssDNA) to yield the fluorescence reporting signals. In the YES logic gate, PCB72 was used as the only input to carry out the logic operation. In the OR, AND, and INHIBIT logic gates, PCB72 and PCB77 were used as the two inputs. The output signals can be visualized by the naked eye under UV light transilluminators or quantified by a microplate reader. Our constructed biosensing platform possesses the merits of multiple combinations of inputs, intuitive digital output, and high flexibility and scalability, which holds great promise for the intelligent detection of different PCBs.

A naphthalenecarboxamide based fluorescent sensor for selective detection of Fe3+ and CN‾: Live cell imaging and INHIBIT logic gate operation

A new fluorescent chemosensor, N,N'-bis(1-hydroxynaphthalene-2-carboxamide)-2-aminobenzylamine (H4L) has been synthesized by a green ionic liquid-based method using tetrabutylammuniom bromide (TBAB) as an environmentally benign reaction medium, and utilized for detection of Fe3+ and CN‾ ions in DMSO-H2O (8:2 v/v) solution. The selective fluorescence response of this chemosensor to Fe3+ is excellent with a fluorescent detection limit (LOD) of 5.16 × 10-7 M. The Job’s plot and ESI-MS analysis indicated a stoichiometric binding ratio of 1:1 between the tri-anionic form of the chemosensor, HL3-, and Fe3+. H4L also displays rapid fluorescence “Turn-On” response to cyanide anion with high selectivity and sensitivity. The detection limit for CN‾ in solution is 2.65 × 10-7 M. Thiscell-permeable probe has also been utilized toimageintracellular Fe3+and CN‾ ions inHeLa cells. Furthermore, the “off–on-off” fluorescence behavior of H4L at 433 nm has been utilized in the fabrication of “INHIBIT” logic gate with Fe3+ and CN‾ as chemical inputs and fluorescence (I433 nm) as output.

Comparison of two pyrazole derived “turn on” fluorescent probes for the recognition of Ga3+

Two fluorescent probes (E)-N'-(2-hydroxybenzylidene)-1-(pyridin-2-yl)-5-(thiophen-2-yl)-1H-pyrazole-3-carbohydrazide (PTP) and (E)-N'-(4-(diethylamino)-2-hydroxybenzylidene)-1-(pyridin-2-yl)-5-(thiophen-2-yl)-1H-pyrazole-3-carbohydrazide (DPTP) were drafted and synthesized to detect Ga3+ through fluorescence turn on strategy. DPTP had a significant fluorescence enhancement compared with PTP after complexing with Ga3+ ions. The detection limit of DPTP (1.21 × 10−8 M) was lower than that of PTP (8.85 × 10−7 M). The Job's plot showed that both sensor PTP and DPTP bound to Ga3+ to form a 1:2 complex. In addition, DPTP could mimic INHIBIT logic function using Ga3+ and PPi being logic inputs and the fluorescence intensity at 466 nm as output signal. Furthermore, the application of DPTP to detect Ga3+ in actual water samples had also achieved satisfactory results and the test papers containing DPTP were fabricated successful for convenient detection of Ga3+.

Anthracene scaffold as highly selective chemosensor for Al3+ and its AIEE activity.

Fluorescent chemosensor, 3-(Anthracen-2-yliminomethyl)-benzene-1,2-diol (ANB) has been synthesized by one-step condensation of 2-aminoanthracene and 2,3-dihydroxybenzaldehyde and characterized using 1H-NMR, FT-IR and Mass spectroscopic techniques. The probe ANB was found to be an efficient 'turn-on' fluorescence chemosensor for the selective detection of Al3+ ion over other metal ions in an aqueous solution. The chemosensor exhibits ~ 27-fold enhancement of emission intensity in presence of Al3+ ion. Fluorescence quantum values for ANB and (Al3+-ANB)-complex are 0.004 and 0.097, respectively. In addition, the binding constant and the limit of detection were found to be 1.22 × 104M-1 and 0.391µM, respectively. The chemosensor ANB binds to Al3+ ions in 2:1 stoichiometric ratio which was supported by Job's plot, 1H-NMR titration and florescence titration. Fluorescence reversibility of the sensor complex was well established by adding EDTA in the same condition and a molecular INHIBIT logic gate was fabricated using this reversible nature of the sensor complex. Additionally, the chemosensor ANB shows a novel aggregation-induced enhanced emission phenomenon, where the aggregate hydrosol of ANB shows enhance emission intensity.

Selective colorimetric signaling of mercury (II) ions using a quinoline-based probe with INHIBIT logic gate behavior and test strip

Selective colorimetric signaling of mercury (II) ions using a quinoline-based probe with INHIBIT logic gate behavior and test strip

Julolidine-hydrazone based chemosensor for detection of Zn2+: Fluorescent in-situ formed Zn2+ complex discriminates PPi from ADP and ATP

In the present investigation, we have designed and synthesised Zn2+ sensitive Julolidine-hydrazone (JSB) based chemosensor, which crystallised in a monoclinic crystal system with P21/c space group. The bare JSB was nonemissive, but in the presence of Zn2+ ions in solution it showed emission, ascribed to the chelation enhanced emission process, which is also utilised to detect Zn2+ in water samples. Comparing the chromaticity coordinates deduced from the emission colors of the JSB-Zn2+ in solution, powder and hybrid polymer thin film, using CIE (Commission Internationale de I'Eclairage 1931) chromaticity diagram, it was found that compared to the emission of the solution, the emission of the powder was red shifted, while that of the thin film was blue shifted. Further, the sensing of Zn2+ showed reversibility in the presence of pyrophosphate (PPi), which allowed quantification of PPi. Interestingly, in addition to the detection of PPi using the in-situ formed JSB-Zn2+ complex, the process was selective and discriminated PPi from ADP and ATP. The detection of PPi was rationalized via a decomplexation reaction, and translated in the construction of INHIBIT logic gate. Additionally, the possible use of the JSB coated sensor paper for the on-site detection of Zn2+ and subsequent JSB-Zn2+ complex for PPi ions has been demonstrated. The experimental results showed good correlation with the theoretical calculations wherever possible.

Cinchona alkaloids - acid, anion-driven fluorescent INHIBIT logic gates with a receptor1-fluorophore-spacer-receptor2 format and PET and ICT mechanisms.

The fluorescent natural products, quinine, quinidine, cinchonine and cinchonidine are demonstrated as H+-enabled, halide-disabled (Cl-, Br- or I-) INHIBIT and INHIBIT-OR combinatorial logic gates in water. More fluorescent natural products with intrinsic logic properties await to be discovered.

Hybrid films composed of ethyl(hydroxyethyl)cellulose and silica xerogel functionalized with a fluorogenic chemosensor for the detection of mercury in water

Ethyl(hydroxyethyl)cellulose (EHEC) and a silica–based xerogel (SBX) were functionalized with a (18–crown–6)–styrylpyridine precursor (1) to obtain the modified polymers EHEC–1 and SBX–1, respectively. Films were obtained and the resulting materials were used as fluorogenic devices for the detection of Hg2+ in water. The films produced from EHEC–1 showed high water retention, making it difficult to apply as a reusable optical chemosensor. Since SBXs are recognized in the literature for their hydrophobicity, a hybrid film composed of EHEC and SBX–1 which did not show water retention was produced and characterized. This system showed rapid response time, outstanding selectivity compared to several other studied metal ions, and sensitivity for the detection of Hg2+ in water. The detection limit for this material using fluorescence technique was 2 ppb (∼10−8 mol L−1). The reversibility of the complex formed between EHEC-SBX–1 film and Hg2+ was demonstrated by the addition of cysteine to the medium. The result obtained also allowed the assembly of INHIBIT and IMPLICATION molecular logic gates, using Hg2+ and cysteine as inputs. The results described in this article have important significance in the development of novel reversible fluorogenic chemosensors and adsorbent materials for the effective removal of Hg2+ ions.

Nitrogen, sulfur, and phosphorus Co-doped carbon dots-based ratiometric chemosensor for highly selective sequential detection of Al3+ and Fe3+ ions in logic gate, cell imaging, and real sample analysis

Heteroatom-doped photoluminescent (PL) carbon dots (CDs) have recently gained attention as optical sensors due to their excellent tunable properties. In this work, we propose a one-pot hydrothermal synthesis of PL nitrogen (N), sulfur (S), and phosphorus (P) co-doped carbon dots (NSP-CDs) using glutathione and phosphoric acid (H3PO4) as precursors. The synthesized NSP-CDs were characterized using different spectroscopic and microscopic techniques, including ultraviolet–visible (UV–Vis) spectroscopy, fluorescence spectroscopy, Fourier-transform infrared (FTIR), X-ray powder diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) analysis. The NSP-CDs exhibited excellent PL properties with green emission at 492 nm upon excitation at 417 nm, a high quantum yield of 26.7%, and dependent emission behavior. The as-prepared NSP-CDs were spherical with a well-monodispersed average particle size of 5.2 nm. Moreover, NSP-CDs demonstrate high PL stability toward a wider pH, high salt ionic strength, and various solvents. Furthermore, the NSP-CDs showed a three-state “off–on–off” PL response upon the sequential addition of Al3+ and Fe3+ ions, with a low limit of detection (LOD) of 10.8 nM for Al3+ and 50.7 nM for Fe3+. The NSP-CD sensor can construct an INHIBIT logic gate with Al3+ and Fe3+ ions as the chemical inputs and emissions as the output mode. Owing to an excellent tunable PL property and biocompatibility, the NSP-CDs were applied for sensing Al3+ and Fe3+ ions as well as live cell imaging. Furthermore, NSP-CDs were designed as PL sensors for detecting Al3+ and Fe3+ ions in real water show their potential application.

Fluorescence 'Turn-on' Dual Sensor for Selective Detection of Cd2+ and H2AsO4- in Water.

A multi responsive fluorescent probe, N',2-bis(E-4-(diethylamino)-2-hydroxybenzylidene)hydrazine-1-carbothiohydrazideV(H2L) has been synthesized through one step condensation method. Probe, H2L shows 'turn-on' dual sensing properties towards Cd2+ and H2AsO4- at two distinct wavelength. The probe (H2L) is spectroscopically characterized and the chemo-sensing mechanism has been demonstrated through 1H NMR, absorption, steady state and time resolved emission study. The most promising advantage of the probe is its application in the one-pot detection of Cd2+ (λem = 462nm) and H2AsO4-(λem = 492nm) where intense emission appears at two different wavelengths and the observed limit of detection (LOD) of H2L towards Cd2+ and H2AsO4- are 2.67 × 10-8M and 5.14 × 10-6M respectively. Further the 'turn-on' emission property of H2L towards Cd2+ is applied to construct INHIBIT logic gate.

AIEE active sensors for fluorescence enhancement based detection of Ni2+ in living cells: Mechanofluorochromic and photochromic properties with reversible sensing of acid and base

Stimuli responsive sensors QI 1 and QI 2 were rationally developed which exhibited diverse features of mutable mechanofluorochromism, reversible photochromism, solvatochromism, aggregation induced emission enhancement (AIEE), and metal ion sensing. After observing the exceptional structural property relationship, sensors were applied for reversible colorimetric and fluorometric determination of Ni2+ with low detection limits of 12 and 17 nM, respectively. Fluorescence emission enhancement based Ni2+ sensing was induced by chelation enhanced fluorescence (CHEF) mechanism. CHEF is triggered by the inhibition of excited state intramolecular proton transfer (ESIPT) and –C=N isomerization. The proposed Ni2+ sensing mechanism was investigated through 1H NMR, FT-IR titration, theoretical studies, and Jobs plots. Further, the developed sensors successfully demonstrated the selective acid-base induced absorption/emission switching through reversible ring-opening/closing and keto–enol tautomerization, evidenced by 1H NMR titration experiments. Additionally, the sensitivity of the sensor QI 1 towards Ni2+ was effectively mimicked in live MCF-7 cells and industrial effluents. Furthermore, monitoring of Ni2+ ions was also accessed through inexpensive and portable sensors’ coated fluorescent films. Finally, an INHIBIT logic gate was fabricated imputing Ni2+ and EDTA as input signals to electronically scrutinize the targeted Ni2+.

Colorimetric probe for sequential chemosensing of mercury(II) and cyanide ions in aqueous media, based on a benzoxadiazole-pyrazolin-5-one glycoconjugate with INHIBIT logic gate response

A new and efficient water-soluble probe L for the sequential detection of Hg2+ and CN− ions has been developed by the condensation of benzoxadiazole and glycoconjugated pyrazoline-5-one. The resulting probe has a water solubility of 45 g/L at room temperature and interacts selectively with Hg2+ ions in water over other cations by changing its color from yellow to red and forming the [L/Hg2+: 2:1] complex. On the other hand, the formed complex [L/Hg2+: 2:1] reacts selectively with CN− anions in an aqueous solution. This reaction is accompanied by a blue shift in wavelength from 545 nm to 472 nm with a color change from red to yellow visible to the naked eye. These reversible and repeatable colorimetric responses of probe L towards Hg2+ and CN− ions can be represented as a molecular logic gate 'IMPLICATION', where Hg2+ and CN− ions are considered as inputs and the absorbance at 472 nm as output. The detection limit for Hg2+ and CN− ions was found to be 2.31 ppb and 4.34 ppb, respectively. From a practical point of view, probe L was used to prepare a test strip based on filter paper treated with L. The resulting test strip selectively changes color after the sequential addition of Hg2+ and CN− ions in water at pH7. These high performances in terms of selectivity and sensitivity allow the probe L to be used for the sequential detection of Hg2+ and CN− ions in aqueous media.

A multi-channel rhodamine-pyrazole based chemosensor for sensing pH, Cu2+, CN– and Ba2+ and its function as a digital comparator

A multi-channel rhodamine-pyrazole-based chemosensor (RhPzNH2) was prepared by a simple synthetic method. The photophysical characteristics of the compound were determined in various organic solvents. The new probe responded discriminately to acidic and basic pHs; in acidic media (pH < 3) it produced a yellow emission centered at 555 nm, while in basic media (pH > 10) its emission was blue with a maximum at 460 nm. Moreover, the new RhPzNH2 sensor responded significantly to Cu2+ by increasing the absorbance at 530 nm (limit of detection (LOD) 7×10−8 M). The stoichiometric ratio of Cu2+ ions and the probe in the complex formed was found to be 1:2, respectively. Possible structural arrangements of the ligand molecule and Cu2+ in the complex were studied by DFT computations and the most likely structural organization was proposed. Furthermore, the responses of the [(RhPzNH2)2-Cu]2+ complex were significant towards Ba2+ and CN–. The substitution of Cu2+ in the complex decreased remarkably the absorbance at 530 nm by 72 %. On the other hand, CN– increased both the absorbance at 530 nm and the fluorescence emission at 550 nm by sharing it in the coordination within the complex increased solubility of the complex, and blocked the energy transfer from the compound to Cu2+. Moreover, the RhPzNH2 mimicked three different INHIBIT logic gates that can be integrated to work as a molecular digital comparator.

An efficient 2-aminothiazolesalicylaldehyde fluorescent chemosensor for Fe2+ ion detection and a potential inhibitor of NUDT5 signaling hormone for breast cancer cell and molecular keypad lock application

A novel thiazole phenol conjugate, 2-aminothiazolesalicylaldehyde (receptor1) was designed and synthesized for the first time through a single step process via Schiff base condensation reaction. The formation of receptor1 was confirmed by FTIR, 13C NMR, and 1H NMR. The IR spectra confirmed the presence of the aldimine formation. It is further supported by the proton NMR, showing the disappearance of aldehyde peaks and the formation of a new imine peak. This is further corroborated by the 13C NMR. The receptor1 complexing with various metal ions were studied through fluorescence spectroscopy showed its selectivity toward Fe2+ ion following a reverse photoinduced electron transfer (PET) process compared to all other potentially competing ions. The receptor1 was applied as a sensor to sense Fe2+ ion in water samples. The detection limit for Fe2+ ion in drinking water was substantially lower (0.003 µM) than the EPA (environmental protection agency) recommendation (5.37 M). The capability of receptor1 in recovering Fe2+ ion in bore water, tap water, and drinking water was up to 99.5%. The receptor1 was also used as a chelating ligand (receptor1) in molecular docking and it was assessed as a potential inhibitor of NUDT5, a silence hormone signaling for breast cancer. The test compound (PDB: 5NWH) showed good affinity toward the target receptor1 with the binding energy of – 5.23 kcal mol−1. Furthermore, the receptor1 showed excellent reversibility property on adding EDTA solution. Due to the marvelous reversible property, a molecular-scale sequential information processing circuit is designed for the multi-task behavior such as ‘Writing-Reading-Erasing-Reading’ in the form of binary logic gate. The consecutive addition of Fe2+ ion and EDTA solution to receptor1 paves a way for the construction of INHIBIT logic gate. Additionally, the receptor1 showed the mimicking behavior of molecular keypad lock.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11696-022-02373-z.

A new 7-(diethylamino)coumarin and 4-(diethylamino)phenol appended unsymmetrical thiocarbohydrazone: Detection of moisture in organic solvent and sequential fluorimetric detection of Cu2+ ions and cysteine

A novel 7-(diethylamino)coumarin and 4-(diethylamino)phenol appended unsymmetrical thiocarbohydrazone (2) was designed and synthesized successfully. The probe 2 forms an interesting three dimensional hydrogen bonded network results in an interesting supramolecular network. The compound, 2 showed fluorometric and colorimetric response towards fluoride anion via deprotonation process due to the presence of phenolic –OH and –NH groups. The anionic species 2-F− was successfully employed for the detection of moisture in organic solvents through the reprotonation process. The moisture detection features of 2-F− were explored by using UV–vis and emission experiments in DMF solvent and the corresponding mechanism was effectively confirmed by 1H NMR spectral analysis. Moreover, the synthesized probe 2 exhibits highly selective and sensitive detection for Cu2+ ions. Furthermore, the 2-Cu2+ ensemble might be used as a highly selective sensor for Cys in the presence of other amino acids without any interference. Further, the potential reversible sensing feature of Cu2+ and Cys by 2 were recognized to develop the INHIBIT logic gate function and on-site detection of Cu2+ ions and Cys by a paper strip applications.

A novel dual naked eye colorimetric and fluorescent pH chemosensor and its ability to execute three INHIBIT based digital comparator

A novel trichromophoric molecular chemosensor Rh–NI-Hz was designed, synthesized and examined for pH detection. It exhibited naked eye observed colorimetric and fluorometric responses to lower and high pH values. The probe sensitivity towards an acidic environment was achieved through the rhodamine part of the molecule where the colour was changed from colourless to red and yellow fluorescence was emitted. On the other hand, in a basic environment, the colour was changed to yellow and a green fluorescence emission was produced. Moreover, the probe mimics a digital comparator by executing three INHIBIT logic gates using H+ and HO− as chemical inputs.