Ingredients In Formulations Research Articles

Fatally impaired glucose digestion by propylene glycol in Aedes aegypti (Diptera: Culicidae) and co-formulation with terpenoids for enhancing attractive toxic sugar baits.

Propylene glycol (PG) demonstrates greater efficacy than other sugar polyols. However, the attributes it confers for toxicity and possible co-formulation with other ingredients are unknown. To evaluate this, α-glucosidase and glucose oxidase reactions were performed in Aedes aegypti (L.) (Diptera: Culicidae) to categorize if PG behaves similarly to prior studied sugar alcohols. A combination of no-choice and choice assays was used to determine effective ratios of PG and sucrose, competitiveness against a control of 10% sucrose, and whether mosquitoes recovered from PG consumption. The final trials included β-cyclodextrin encapsulated cinnamon leaf oil, clove stem oil, patchouli oil, garlic oil, cedarwood oil, and papaya seed oil formulated with 5% sucrose + 5% PG. PG functioned as a linear competitive inhibitor of α-glucosidase. The efficacy of PG was synergized by co-ingestion with equivalent ratios of sucrose. Unlike the high diuretic response to other sugar alcohols, PG resulted in diminished excretion regardless of being co-formulated with sucrose or terpenoids. PG is not especially competitive against unadulterated sugar meals but is likewise not clearly repellent. Although mosquitoes did not recover from ingestion of the glycol meals, there was no indication that mortality would continue to accumulate once the treatments were removed. Of the terpenoids tested, cinnamon and patchouli caused ~50% or less mortality; garlic, cedarwood, and clove caused 80-90% mortality; and papaya seed caused 100% mortality, exceeding all other test groups and the formulation blank. PG is a useful supporting ingredient in attractive toxic sugar bait formulations with flexibility in formulation.

An overview of the chemistry of condensation curing silicone sealants and adhesives

Condensation curing silicone sealants and adhesives are materials that are used to connect substrates to each other in various industries, especially where harsh conditions are limiting the use of organic materials. Chemistry of condensation curing silicones is reviewed in this publication through an historical perspective, which provides an insight into the challenges faced by the early formulators. The impact of the formulation ingredients can be very significant on the uncured, curing and/or cured properties of the material. Their main influence is described with a special focus on the main curing systems, i.e., acetoxy, alkoxy, oxime, amine, acetone, and amide. Cure characteristics are summarized and compared across their chemistries. Alkyl tin carboxylates and alkoxy titanium catalysts are the most used condensation catalysts. They are acting through a different mechanism, which is compared briefly. Typical features and principles about one-part and two-part silicones are described and related to their physicochemical properties. Finally, a brief review of the latest developments and trends is depicted.

Formulation, Evaluation, and Characterization of Indapamide Niosomes

Niosomes, also known as nonionic surfactant vesicles, are one of the many carriers used to carry drug molecules to their target sites. They can hold both hydrophilic and hydrophobic medicines. Indapamide is an angiotensin II type 1 receptor (AT1) antagonist medication primarily used to treat high blood pressure. Niosomes containing Indapamide were created utilizing the thin film hydration process with various cholesterol concentrations and nonionic surfactants (span 60). All noisome formulations were assessed for entrapment efficiency, drug content, reproducibility, vesicular diameter, shape and size distribution microphotography, FTIR analysis, and in vitro release experiments. The results indicate that in all of the created niosomal formulations, as the surfactant content increases, the entrapment efficiency also increases. The drug content ranged between 90.06±0.57 and 96.15±0.42, with a low standard deviation. Niosomes range in size from 0.280±0.098µm to 0.299±0.044µm and have a spherical shape. The IR spectrum analysis indicated no interaction between the medication and the formulation ingredients. Membrane diffusion cells were used to study the in vitro dissolution parameters. The results demonstrate that formulation F6 had a better-controlled release action than other formulations, with an 'n' value of 0.917, indicating that the medication was released using zero-order kinetics.

In vitro microrhizome induction in Acorus calamus L., a commercially important aromatic medicinal plant

Acorus calamus L., is a leading aromatic medicinal plant that produces pungent aromatic rhizomes that are valued worldwide as an important herbal medicine and is one of the main ingredients in several polyherbal formulations used for neurological and metabolic disorders. The present investigation aims to develop an efficient protocol for in vitro microrhizome induction in A. calamus for the large-scale production of disease-free planting material for commercial purposes. In vitro derived shoots were initiated on MS medium supplemented with different concentrations of sucrose alone (3–10 %) and different concentrations of sucrose (6 % and 7 %) with varying concentrations of BAP alone (0.5–2 mg/L) and combinations with IAA (0.5 and 1 mg/L) and NAA (0.5 and 1 mg/L) were carried out for the microrhizome induction experiments. In different concentrations of sucrose used, healthy and disease-free microshoots of A. calamus were obtained in MS media supplemented with 7 % sucrose followed by 6 % sucrose. The highest shoot length (24.79 ± 0.03 cm) and microrhizome size (4.98 ± 0.03 cm length and 399.60 ± 0.37 mg fresh weight) were obtained in MS solid medium supplemented with 1 mg/L BAP and 0.5 mg/L NAA with 7 % sucrose followed by the same hormone concentration with 6 % sucrose (shoot length-23.68 ± 0.03 cm, microrhizome length- 4.68 ± 0.03 cm and fresh weight of microrhizome-376.60 ± 0.57 mg). The developed protocol can be used for large-scale production of disease-free propagules without rooting and acclimatization and enhance the production of true-to-type planting material.

Polyglutamate: Unleashing the Versatility of a Biopolymer for Cosmetic Industry Applications

Polyglutamic acid (PGA), a biopolymer comprising repeating units of glutamic acid, has garnered significant attention owing to its versatile applications. In recent years, microbial production processes have emerged as promising methods for the large-scale synthesis of PGA, offering advantages such as sustainability, efficiency, and tailored molecular properties. Beyond its industrial applications, PGA exhibits unique properties that render it an attractive candidate for use in the cosmetic industry. The biocompatibility, water solubility, and film-forming characteristics of PGA make it an ideal ingredient for cosmetic formulations. This article explores the extensive potential cosmetic applications of PGA, highlighting its multifaceted role in skincare, haircare, and various beauty products. From moisturizing formulations to depigmentating agents and sunscreen products, PGA offers a wide array of benefits. Its ability to deeply hydrate the skin and hair makes it an ideal ingredient for moisturizers, conditioners, and hydrating masks. Moreover, PGA’s depigmentating properties contribute to the reduction in hyperpigmentation and uneven skin tone, enhancing the overall complexion. As the demand for sustainable and bio-derived cosmetic ingredients escalates, comprehending the microbial production and cosmetic benefits of PGA becomes crucial for driving innovation in the cosmetic sector.

Critical review of Swetha Chandana (Santalum album l.) in Nighantus and Caraka Samhita

Chandana also known as Indian sandalwood, scientifically known as Santalum album Linn, is a medium sized tree belongs to the family Santalaceae. It is one of the oldest and most valuable sources of natural smell and has significant medical and commercial value. Chandana is used as a single drug and ingredient of numerous formulations for the betterment of lifestyle and treatment. Although, numerous studies regarding Chandana there still lies scope for evaluation and analysis on this topic.

Bile acid/fatty acid integrated nanoemulsomes for nonalcoholic fatty liver targeted lovastatin delivery: stability, in-vitro, ex-vivo, and in-vivo analyses

ABSTRACT Background Controlled and targeted drug delivery to treat nonalcoholic fatty liver disease (NAFLD) can benefit from additive attributes of natural formulation ingredients incorporated into the drug delivery vehicles. Methods Lovastatin (LVN) loaded, bile acid (BA) and fatty acid (FA) integrated nanoemulsomes (NES) were formulated by thin layer hydration technique for synergistic and targeted delivery of LVN to treat NAFLD. Organic phase NES was comprised of stearic acid with garlic (GL) and ginger (GR) oils, separately. Ursodeoxycholic acid and linoleic acid were individually incorporated as targeting moieties. Results Stability studies over 90 days showed average NES particle size, surface charge, polydispersity index, and entrapment efficiency values of 270 ± 27.4 nm, −23.8 ± 3.5 mV, 0.2 ± 0.04 and 81.36 ± 3.4%, respectively. Spherical NES were observed under a transmission electron microscope. In-vitro LVN release depicted non-fickian release mechanisms from GL and GR oils-based NES. Ex-vivo permeation of BA/FA integrated NES through isolated rat intestines showed greater flux than non-integrated ones. Conclusion Liver histopathology of experimental rats together with in-vivo lipid profiles and liver function tests illustrated that these NES possess the clinical potential to be promising drug carriers for NAFLD.

Enhancing the Antioxidant, Anti-tyrosinase and Anti-elastase Activity of Pigmented Rice Extracts by Sonication, Heating, Enzyme Digestion and Fermentation

In the modern cosmetics industry, there exists a major trend for replacement of synthetic chemicals with plant-based ingredients in skincare formulation. This research aimed to assess the efficiency of different methods to extract bioactive compounds (as measured by total phenolic content; TPC and total flavonoid content; TFC) responsible for three important cosmeceutical properties (antioxidation, anti-tyrosinase, and anti-elastase) from selected rice grains. The grains of two varieties of purplish-black rice (Neaw Leum Pua; NLP) and black rice (Hom Nil; HN) were extracted with phosphate buffered saline (PBS) and then supplementary treated with sonication, heating, and proteolytic enzyme digestion or fermented with Aspergillus niger. The results showed that sonication slightly enhanced TPC and TFC, but significantly increased antioxidant capacity and anti-tyrosinase activity. The high temperature of 60℃ and 100℃ significantly increased antioxidant capacity based on FRAP assay but reduced that based on DPPH radical scavenging assay. Anti-tyrosinase activity was enhanced by high temperature but that of anti-elastase was inhibited. The addition of papain to the seed extracts slightly enhanced anti-elastase and anti-tyrosinase activity. Notably, fermented rice grains exhibited much greater yields of TPC and TFC, and higher biochemical activities than other methods. The fermented rice exhibited the highest TFC after 3 days; the highest TPC, antioxidant capacity, and tyrosinase inhibition after 6 days; and the highest elastase inhibition activity after 9 days. Therefore, fermentation was the most promising technique to enhance all three desirable properties for cosmetic ingredients when pigmented rice was used as the raw material.

Simultaneous geraniol and citronellol transesterification using Pseudomonas fluorescens lipase for the production of fragrance and flavour esters: A kinetic study

This study aims to develop, for the very first time, a kinetic model for the simultaneous transesterification of geraniol and citronellol using Pseudomonas fluorescens lipase to produce their respective esters. This allows us to test the feasibility of the bioprocess and comprehend the enzyme behaviour, fine-tune process efficiency, and predict process performance. Geranyl and citronellyl esters are key ingredients in fragrance and flavour formulations in the food sector, as well as bioactive compounds for pest and insect repellents in the agricultural sector. Unlocking a greener and sustainable production of these terpene esters directly from plant-derived essential oil is crucial to meet the 2050 net zero targets to produce bio-based chemicals. The simultaneous transesterification of geraniol and citronellol in a batch reactor successfully achieved conversions of 66 % for citronellol and 94 % for geraniol within 5 h. Key optimum conditions were 1:8 molar ratio (geraniol:vinyl acetate), an enzyme loading of 10 wt % of geraniol, temperature of 40 °C, and a stirring speed of 250 RPM. The results indicated that simultaneous transesterification followed a ternary complex mechanism with competitive inhibition by alcohol. Initial testing of rose geranium oil under optimised conditions yielded 32 % citronellol and 50 % geraniol conversion after 30 h. Overall, this study shows promise as a green pathway utilising enzyme catalysis to selectively produce esters from diverse terpene mixtures and can be extended to terpene alcohols derived from flower essential oils.

Mechanistic modeling of twin screw wet granulation for pharmaceutical formulations: Calibration, sensitivity analysis, and model-driven workflow

Wet granulation, a particle size enlargement process, can significantly enhance the critical quality attributes of powders and improve the ability to form tablets in pharmaceutical manufacturing. In this study, a mechanistic-based population balance model is applied to twin screw wet granulation. This model incorporated a recently developed breakage kernel specifically designed for twin screw granulation, along with nucleation, layering, and consolidation. Calibration and validation were performed on Hydrochlorothiazide and Acetaminophen formulations, which exhibit different particle size and wettability characteristics. Utilizing a compartmental experimental dataset, a comprehensive global sensitivity analysis identified critical inputs impacting quality attributes. The study revealed that the nucleation rate process model, effectively represented particle size distributions for both formulations. Adjustments to nucleation and breakage rate parameters, influenced by material properties and screw configuration, improved the model’s accuracy. A model-driven workflow was proposed, offering step-by-step guidelines and facilitating PBM model usage, providing essential details for future active pharmaceutical ingredient (API) formulations.

Potencial Antifúngico do Extrato de Arnica Brasileira (Lychnopora ericoides) e Alecrim (Rosmarinus officinalis) no Controle de Malassezia furfur capilar

This exploratory study aimed to assess the antifungal potential of Brazilian Arnica (Lychnopora ericoides) and Rosemary (Rosmarinus officinalis) extracts against the fungus Malassezia furfur, focusing on their possible application in combating capillary oiliness. Malassezia furfur is a fungus commonly associated with seborrheic dermatitis and other skin disorders, including excessive capillary oiliness. Brazilian Arnica and Rosemary extracts emerge as potential therapeutic agents due to their antifungal and oil-regulating properties. Brazilian Arnica and Rosemary were chosen for their phytotherapeutic properties and the antifungal potential previously observed in related research. Methods included obtaining the extracts through specific procedures, such as collecting and drying the aerial parts of Brazilian Arnica and macerating the Rosemary leaves. Certified strains of Malassezia furfur were used to determine the Minimum Inhibitory Concentration (MIC) of the extracts, demonstrating remarkable antifungal activity. The results indicated that Brazilian Arnica and Rosemary extracts exhibited properties that may contribute to controlling sebum production on the scalp. This dual benefit, combining antifungal activity against Malassezia furfur and the ability to control capillary oiliness, suggests that these extracts have potential as ingredients in hair formulations. These results highlight the importance of these extracts as multifunctional therapeutic agents in the treatment of scalp skin disorders. Further research is needed to fully explore their benefits and potential applications in dermatological clinical practice.

Effects of Artemisia dracunculus powder on dough rheology and quality properties as a novel ingredient in bread formulation.

Tarragon has a great potential to be a healthy functional food ingredient thanks to its rich antioxidant, phenolic compounds, and nutrient content. The possibility of enriching bread with tarragon was investigated. For this aim, tarragon powder was used at the rates of 0, 2, 4 and 6% instead of wheat flour. In this study, the effects of substitution on the rheological properties of bread dough and color, total phenolic content, antioxidant activity, texture, sensory, and Fourier transform infrared (FT-IR) analysis of bread samples were performed. The composition of tarragon powder showed significant protein (23.16%), crude fiber (7.4%), antioxidant (48.22 ± 0.11%), and total phenolic content (511.66 ± 1.56 mg GAE/100 g). Bread samples with increased fiber and protein content were obtained by adding tarragon powder to the bread formulation. The major differences in the FT-IR absorbance spectra for the bread samples were not observed. Additionally, tarragon powder significantly increased the antioxidative properties of breads (p < 0.05). Adding up to 4% tarragon powder to the bread formulation increased the sensory scores of the breads.

Evaluation of the technological and compositional features of pancakes fortified with Acheta domesticus

This work focused on the nutritional and technological evaluation of pancakes fortified with increasing Acheta domesticus powder (AP) levels. Scientific literature underscores AP's potential as an ingredient in bakery formulations due to its notable fiber and protein content. Thus, we formulated different pancake samples by replacing wheat flour (WF) with 10, 20, and 30% of AP, denoted as AP10, AF 20, and AP30, respectively, alongside a control sample (CP). We evaluated Water Absorption Capacity (WAC) and Water Solubility Index (WSI) on WF, AP, different WF and AP mixes, and batters' rheology. Lastly, we assessed the pancakes' physical, technological, and compositional parameters. The consistency index, flow index, and density increased from CP to AP30. A similar trend was observed for the textural parameter of hardness and chewiness, while cohesiveness slightly decreased. The pancake diameter and height were higher in fortified samples than in CP. A noticeable shift from brighter to darker color was observed from CP to AP30, with an increase in the a* (toward red) and b* parameters (toward blue). AP can represent a valuable ingredient for baked good fortification.

Multi-biomarker approach to evaluate the toxicity of chlorpyrifos (active ingredient and a commercial formulation) on different stages of Biomphalaria straminea

Biomphalaria straminea is a freshwater gastropod native to South America and used in toxicological assessments. Our aim was to estimate 48 h-LC50 and sub-chronic effects after the exposure to low concentrations of chlorpyrifos as commercial formulation (CF) and active ingredient (AI) on B. straminea adult, embryos and juveniles. Concentrations between 1 and 5000 μg L−1 were chosen for acute exposures and 0.1 and 1 μg L−1 for the sub-chronic one. After 14 days biochemical parameters, viability and sub-populations of hemocytes, reproductive parameters, embryotoxicity and offspring' survival were studied. Egg masses laid between day 12 and 14 were separated to continue the exposure and the embryos were examined daily. Offspring' survival and morphological changes were registered for 14 days after hatching. 48 h-LC50, NOEC and LOEC were similar between CF and AI, however the CF caused more sub-lethal effects. CF but not the AI decreased carboxylesterases, catalase and the proportion of hyalinocytes with respect to the total hemocytes, and increased superoxide dismutase and the % of granulocytes with pseudopods. Also CF caused embryotoxicity probably due to the increase of embryos' membrane permeability. Acetylcholinesterase, superoxide dismutase, hemocytes sub-populations, the time and rate of hatching and juveniles' survival were the most sensitive biomarkers. We emphasize the importance of the assessment of a battery of biomarkers as a useful tool for toxicity studies including reproduction parameters and immunological responses. Also, we highlight the relevance of incorporating the evaluation of formulations in order to not underestimate the effects of pesticides on the environment.

Diflubenzuron Mass Concentration Determination Method in Pesticides Using High-Performance Liquid Chromatography.

Pesticides are actively used in agriculture to protect crops from pests, and accurate determination of the concentration of their active ingredient is critical to ensuring their efficacy and safety. The research aims to develop a methodology that will enable the accurate determination of the mass concentration of diflubenzuron, a widely used active ingredient in pesticide formulations. This is achieved through the use of high-performance liquid chromatography (HPLC). The proposed method for determining the mass concentration of diflubenzuron using HPLC was developed through a series of experiments and optimisation steps. The sample preparation procedure was optimised to extract the maximum amount of diflubenzuron from the pesticide formulation. The chromatography conditions were optimised to separate diflubenzuron from other components in the sample matrix. A calibration approach was established for accurate quantification of diflubenzuron in the sample based on peak area measurements. The developed method can be widely used in agriculture, where accurate determination of diflubenzuron concentrations in pesticide formulations is critical for proper application and regulatory compliance. Both regulatory organizations and pesticide manufacturers can use it for batch-release testing and quality control to evaluate the efficacy and safety of pesticide products. In addition, the method can serve as a valuable tool for researchers and analysts working with pesticide residues in crops and environmental monitoring studies. The optimised sample preparation, chromatography conditions, and calibration approach provide reliable and reproducible results.

A systematic review on exploration of therapeutic potential of Aparajita (Clitoria Ternatea Linn)

Aparajita (Clitoria ternatea Linn), is an herb widely distributed in the humid, lowland tropics of Africa, Asia, and Central America. Aparajita is known for its katu, tikta, kashaya rasa, and vishaghna (which alleviate toxins) properties. It is used in the treatment of various diseases and is a major ingredient in various anti-poisonous formulations. Pharmacologically, it has actions like insecticidal, cholinergic, antioxytocic, antihistaminic, analgesic, diuretic, purgative, laxative, and abortifacient. A systematic review is attempted based on preclinical studies to prepare a strong platform for the clinical utilisation of Aparajita as a main drug. The research papers reporting the study of Clitoria ternatea Linn in animals (In vivo and in vitro) were searched for systematic review. The literature search was performed using keywords like 'preclinical studies on Aparajita,' 'animal studies on Aparajita,' and 'pharmacological studies on Aparajita,' in combination with various effects in animals, in the following databases: PubMed/Medline, Scopus, Science Direct, Google Scholar, AYUSH Research Portal, and DHARA for studies published up to December 2021. Many preclinical studies reported that Clitoria ternatea Linn has diuretic, nootropic, anti-asthmatic, anti-inflammatory, analgesic, antipyretic, antidiabetic, antilipidemic, antioxidant, and wound healing properties. In the contemporary view, several pharmacological studies and preclinical studies prove the effectiveness of Clitoria ternatea Linn in various diseases. However, very few clinical studies of Clitoria ternatea Linn were done. Further clinical research should be conducted to establish the therapeutic potential of the drug Aparajita.

New drugs for small animals in 2023

In 2023, 2 novel pharmaceutical agents for small animals were released on the German market: the structural but non-functional analog of the inhibitory neurotransmitter gamma-aminobutyric acid pregabalin with an anxiolytic active component and the dopamine agonist ropinirole in form of eye drops to induce vomiting. Two established active veterinary pharmaceutical ingredients became available for additional species: The phosphorus compound butafosfan was additionally approved for horses, dogs and cats and the mineral sodium chloride as an isotonic sodium chloride solution was also approved for rabbits and guinea pigs. In addition, for small animals, there were new releases of an agent (dexamethasone) in a novel pharmaceutical formulation, one drug with a new route of administration (hydrocortisone aceponate), one drug with a new content of the active ingredient (firocoxib) as well as one veterinary drug with a new combination of active ingredients in a novel pharmaceutical formulation (gentamicin+mometasone+posaconazole). Furthermore, one combination of active ingredients (diprophylline+heptaminol) is available on the market for small animals again.

Effect of Dhupana (medicated fumigation) on the microbial concentration in the indoor air in Kumaragara (pediatric OPD and IPD): an experimental study

Abstract Introduction: Bioaerosols causing infectious diseases are a major concern for the environmental safety in hospital indoors. Chemical fumigation used for controlling infectious bioaerosols leads to several health hazards such as allergic reactions, hyperbilirubinemia, carcinogenicity, and toxicity. Corrosion of metal surfaces, bleaching of fabrics, risk of accidental consumption, high cost, unpleasant odor, flammability, and formation of toxic gases are other concerns. In Ayurveda, Dhupana (medicated fumigation) is advocated for controlling invisible agents capable of disease transmission. It can be a safer alternative to chemical fumigation. However, many ingredients of classical Dhupana preparations are rare or impractical to use; hence, a novel “Shodashanga Dhupa” (16-ingredient fumigation preparation) was formulated using easily accessible ingredients of classical Dhupana formulations. Materials and Methods: The antimicrobial and antifungal activities of Shodashanga Dhupa were ascertained by enumerating colony-forming units (CFUs) in the passive air samples obtained from hospital rooms before and after 1 h of Dhupana. These samples were collected on suitable agar media, and the settle plate method was carried out. Results: There was improvement in the Index of Microbial Air Contamination and a statistically significant reduction in bacterial CFUs in the pediatric outpatient department (P = 0.000218) and in the Panchakarma therapy room (P = 0.000218). There was a statistically significant reduction in fungal CFUs (P = 0.0137) in the pediatric outpatient department and in the Panchakarma therapy room (P = 0.00109) after 1 h of Dhupana. Conclusion: The study suggests that fumigation with common herbs and spices is effective in decreasing bacterial and fungal bioaerosol concentration in the indoor air of hospital rooms, and it can be used as an economical, hassle-free, and safe method of disinfection.

High-Value Compounds in Papaya By-Products (Carica papaya L. var. Formosa and Aliança): Potential Sustainable Use and Exploitation.

Food waste is a global and growing problem that is gaining traction due to its environmental, ethical, social, and economic repercussions. Between 2022 and 2027, the worldwide papaya market is expected to have a huge increase, meaning a growth in organic waste, including peels and seeds. Thus, this study evaluated the potential use of peels and seeds of two mature papaya fruits as a source of bioactive compounds, converting these by-products into value-added products. Proximate analysis (AOAC methods), mineral content (ICP-MS), free sugars (HPLC-ELSD), fatty acid composition (GC-FID), vitamin E profile (HPLC-DAD-FLD), and antioxidant activity (DPPH and FRAP assays) were evaluated. Both by-products showed high total protein (20-27%), and dietary fiber (32-38%) contents. Papaya peels presented a high ash content (14-16%), indicating a potential application as a mineral source. 14 fatty acids were detected, with α-linolenic acid (30%) as the most abundant in the peels and oleic acid (74%) in the seeds. Both by-products showed high antioxidant activity. Papaya by-products display great potential for industrial recovery and application, such as formulation of new functional food ingredients.

An ecotoxicological assessment of a strigolactone mimic used as the active ingredient in a plant biostimulant formulation

A risk assessment on the aquatic toxicity of the plant biostimulant strigolactone mimic (2-(4-methyl-5-oxo-2,5-dihydro-furan-2-yloxy)-benzo[de]isoquinoline-1,3-dione (SL-6) was performed using a suite of standardised bioassays representing different trophic groups and acute and chronic endpoints. In freshwater, three trophic groups of algae, crustacea and fish were used. Whilst in seawater, algae (unicellular and macroalgae), Crustacea and Mollusca were employed. In addition, the genotoxicity of SL-6 was determined with the comet assessment performed on unicellular marine algae, oysters, and fish embryos. This was the first time ecotoxicity tests have been performed on SL-6. In freshwater, the lowest LOEC was measured in the unicellular algae at 0.31 mg/L SL-6. Although, similar LOEC values were found for embryo malformations and impacts on hatching rate in zebrafish (LOEC 0.31–0.33 mg/L). Consistent malformations of pericardial and yolk sac oedemas were identified in the zebrafish embryos at 0.31 mg/L. In marine species, the lowest LOEC was found for both Tisbe battagliai mortality and microalgae growth at an SL-6 concentration of 1.0 mg/L. Significant genotoxicity was observed above control levels at 0.0031 mg/L SL-6 in the unicellular algae and 0.001 mg/L SL-6 in the oyster and zebrafish larvae. When applying the simple risk assessment, based on the lowest NOECs and appropriate assessment factors, the calculated predicted no effect concentration (PNEC), for the ecotoxicity and the genotoxicity tests were 1.0 µg/L and 0.01 µg/L respectively.